大鼠肝部分切除后血清可促进培养结肠癌细胞的生长。

K P de Jong, M A Brouwers, M L van Veen, M Brinker, E G de Vries, T Daemen, G L Scherphof, M J Slooff
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引用次数: 12

摘要

荷瘤大鼠随机分为70%肝部分切除组和假手术组。在第1、3、14天,取门脉血清和全身血清,在5%、10%、20%或50%血清的存在下培养结肠癌细胞。检测肿瘤细胞的增殖和表皮生长因子受体(EGFr)的表达。肝切除术后用门脉血清培养的肿瘤细胞增殖率比假手术后高25-40%,但术后第1天和第14天用血清培养的肿瘤细胞增殖率不高。术后第14天血清培养的CC 531细胞,全身性肝切除血清比假全身性血清培养的CC 531细胞增殖率高30%。这些影响不是由EGFr mRNA和蛋白质水平的变化介导的,因为所使用的结肠癌细胞在三种检测方法中都没有显示出EGFr活性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Serum obtained from rats after partial hepatectomy enhances growth of cultured colon carcinoma cells.

Tumour-bearing rats were randomized to a 70% partial hepatectomy or a sham operation. At days 1, 3 or 14, portal and systemic serum was obtained and colon carcinoma cells were cultured in the presence of 5, 10, 20 or 50% serum. Proliferation and epidermal growth factor receptor (EGFr) expression was measured in tumour cells. Proliferation was 25-40% higher in tumour cells cultured with portal serum after hepatectomy than after sham operation when using serum obtained at day 3, but not days 1 and 14 after operation. In cultures with serum obtained at day 14 after operation CC 531 cells showed a 30% higher proliferation rate with systemic hepatectomy serum than CC 531 cells with sham systemic serum. These effects were not mediated by a change in EGFr mRNA and protein levels as the used colon carcinoma cells did not reveal EGFr activity by any of the three detection methods used.

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