International Microbiology最新文献

{"title":"Differences of microbial growth and biofilm formation among periprosthetic joint infection-causing species: an animal study.","authors":"Mehmet Batu Ertan, Mehmet Yağız Ayduğan, Ebru Evren, İrem İnanç, Esra Erdemli, Bülent Erdemli","doi":"10.1007/s10123-024-00629-0","DOIUrl":"10.1007/s10123-024-00629-0","url":null,"abstract":"<p><strong>Purpose: </strong>The most frequently used surgical procedures for periprosthetic joint infections (PJIs) are debridement, antibiotics, and implant retention (DAIR), as well as single- or two-stage revision arthroplasty. The choice of surgery is made depending on the full maturation of the biofilm layer. The purpose of this study was to evaluate the biofilm formation and microbial growth using common PJI-causing agents and compare its development on the implant surface.</p><p><strong>Methods: </strong>The in vivo study was performed using 40 Sprague-Dawley rats divided into five groups (n = 8/group): Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa, Candida albicans, and control. Six standard titanium alloy discs were placed into the subcutaneous air pouches of the interscapular areas of the rats. After the inoculation of microorganisms, disc and soft tissue cultures were collected at 2-week intervals for 6 weeks, and the microbial load and the microscopic appearance of the biofilm were compared.</p><p><strong>Results: </strong>The disc samples from the S. aureus group had the highest infection load at all time points; however, in soft tissue samples, this was only observed at week 4 and 6. Electron microscopic images showed no distinctive differences in the biofilm structures between the groups.</p><p><strong>Conclusion: </strong>S. aureus microbial burden was significantly higher in implant cultures at week 2 compared to other PJI-causing agents examined. These results may explain the higher failure rate seen if the DAIR procedure was performed at < 3-4 weeks after the PJI symptom onset and support the observation that DAIR may not be effective against PJIs caused by S. aureus.</p>","PeriodicalId":14318,"journal":{"name":"International Microbiology","volume":" ","pages":"1483-1493"},"PeriodicalIF":2.3,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143005037","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Epidemiology, risk factors, and clinical characteristics of amoebic liver abscess: a systematic review and meta-analysis from India.","authors":"Syed Muhammad Sarosh Ghalib, Faizeaab Hashmi, Umra Fatima Zuberi, Sana Aqeel","doi":"10.1007/s10123-025-00645-8","DOIUrl":"10.1007/s10123-025-00645-8","url":null,"abstract":"<p><p>Amoebiasis and its extraintestinal manifestation amoebic liver abscess (ALA) is an endemic disease in tropical and subtropical countries. However, no meta-analysis on the prevalence, associated risk factors, and clinical markers has been performed. This study aims to bridge this research gap. Based on our inclusion criteria, 18 studies were selected for analysis. Using the \"meta\" package in RStudio, forest plots, regression analysis, and funnel plots were used to represent the proportional meta-analysis, significance of the correlation, and quality and publication bias of the included studies, respectively. A high prevalence of ALA (67%, p = 1.762 × 10^-15) was noted in cases of liver abscess. ALA was significantly more prevalent in males (86%, p = 2.25 × 10^-8), individuals with poor socioeconomic conditions (75%, p = 0.02496), history of alcohol consumption (63%, p = 1.421 × 10^-8), and diabetic comorbidity (35%, p = 0.01169). There was a significant correlation with abdominal pain (91%, p = 1.286 × 10^-12), infected right lobe (81%, p = 0.002), fever (73%, p = 2.567 × 10^-12), hepatomegaly (69%, p = 1.223 × 10^-5), anemia (69%, p = 1.223 × 10^-5), and jaundice (11%, p = 0005258) in the affected individuals. We conclude that the above-stated risk factors and clinical markers are significantly associated with ALA and suggest that medical professionals in endemic regions must attribute liver abscess to be etiologically amoebic and correlate the disease manifestation with the risk factors and clinical markers. Further, research is needed to better understand disease manifestation.</p>","PeriodicalId":14318,"journal":{"name":"International Microbiology","volume":" ","pages":"1669-1685"},"PeriodicalIF":2.3,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143467939","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impacts of different salt concentrations on the morphology, physiology, and antioxidant properties of the rice-field cyanobacterium Nostochopsis lobatus HKAR-21.","authors":"Ashish P Singh, Amit Gupta, Jyoti Jaiswal, Prashant R Singh, Rajeshwar P Sinha","doi":"10.1007/s10123-025-00638-7","DOIUrl":"10.1007/s10123-025-00638-7","url":null,"abstract":"<p><p>The physiological and biochemical responses of organisms, including cyanobacteria, are greatly influenced by environmental change. Salinity significantly affects global rice output, and cyanobacteria are major contributors to rice productivity. Therefore, in our study, we explore the effects of varying concentrations of three (i.e., MgSO₄, CaCl₂, K₂HPO₄) agriculturally important salts on the biomass, growth, and biochemical status of the rice-field cyanobacterium Nostochopsis lobatus HKAR-21. A significant decrease in the photopigment activity, growth, and effective quantum yield of photosystem II (PSII) (F_v/F_m) was observed in the cells treated with 0 μM K₂HPO₄ and 0 μM MgSO₄. There was minimum effect on the growth and photopigment in samples treated without CaCl₂ (0 μM CaCl₂). Phycobiliproteins (PBPs) and carbohydrate content were largely regulated by MgSO₄. However, K₂HPO₄ had an optimum effect on all three macromolecular components. Brightfield imaging revealed that each salt had a characteristic role in the morphology of the cells. Additionally, in vitro lipid peroxidation with in vivo reactive oxygen species (ROS) and lipid peroxidation were examined with the help of DCFH-DA and Nile red fluorescence, respectively. The antioxidant potential of the carotenoid was checked by using DPPH and ABTS assays. Our study conclusively highlights that the increasing salt concentration will have a negative impact on the cyanobacterium growth which in turn will affect the productivity of rice paddy fields which is one of the most important crops on which more than a billion people are dependent.</p>","PeriodicalId":14318,"journal":{"name":"International Microbiology","volume":" ","pages":"1617-1633"},"PeriodicalIF":2.3,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143425453","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unveiling the potential of bioslurry and biogenic ZnO nanoparticles formulation as significant bionanofertilizer by ameliorating rhizospheric microbiome of Vigna radiata.","authors":"Abhinav Singh, Ritika Chauhan, Ram Prasad, Amay A Agrawal, Pankaj Sah, Arti Goel","doi":"10.1007/s10123-025-00649-4","DOIUrl":"10.1007/s10123-025-00649-4","url":null,"abstract":"<p><p>Advancements in nanotechnology, particularly the use of bionanofertilizers, show promise for sustainable agriculture by enhancing soil health and reducing reliance on conventional fertilizers. This study explored the impact of a bioslurry and biogenic zinc oxide (ZnO) nanoparticle formulation on microbial diversity in the rhizosphere of Vigna radiata (mung bean) using 16S rRNA sequencing. High-quality reads from both untreated and treated soil samples revealed a dominance of Archaea, though its proportion was reduced in the treated sample (66% in untreated, 58% in treated). The treated soil showed an increased abundance of beneficial bacterial phyla, including Acidobacteria (+ 6%), Actinobacteria (+ 2%), and Firmicutes (+ 2%). Notably, Acidobacteria-6 and Chloroacidobacteria, essential for nutrient cycling, were enriched in treated soil. Alpha diversity (Chao1 and Shannon indices) was lower in treated samples, indicating selective enhancement of beneficial microbes. Functional analyses like Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt) and Statistical Analysis of Taxonomic and Functional Profiles (STAMP) analysis highlighted increased pathways related to motility, chemotaxis, and metabolic processes in the treated soil. These findings suggest that ZnO NPs and bioslurry treatment at 250 ppm improves soil microbial composition and functional attributes, supporting its potential as a bionanofertilizer for soil health restoration and enhanced plant growth.</p>","PeriodicalId":14318,"journal":{"name":"International Microbiology","volume":" ","pages":"1725-1737"},"PeriodicalIF":2.3,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143541292","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of the mechanism of tripropylamine-enhanced lycopene accumulation in Blakeslea trispora through transcriptome.","authors":"Aizhong Han, Yue Liu, Ziang Gao, Jinghan Wang, Tianxiang Zhang, Ruigang Zhang, Gongzhan Sun, Yanlong Wang","doi":"10.1007/s10123-025-00681-4","DOIUrl":"10.1007/s10123-025-00681-4","url":null,"abstract":"<p><p>Lycopene is a carotenoid with strong antioxidant properties. Treatment with tripropylamine (TPA) greatly increases lycopene production by Blakeslea trispora, but the mechanism of this effect remains unclear. In this study, a reference-free transcriptome was used to analyze changes in gene expression in B. trispora after treatment with TPA. Downregulated genes were mostly involved in primary and secondary metabolic pathways, whereas upregulated genes were mostly related to autophagy and nucleotide excision repair. In addition, after TPA treatment, the mycelium swelling and the number of spores decreased. Our study suggests that TPA causes DNA damage, which upregulates the expression of nucleotide excision repair genes Xpb, Xpg, Csb, Polr2, and genome stability regulator gene Rmi1, as well as downregulation of Lsm2 and Dars2 and upregulation of G10, which are related to mRNA spliceosomes, leaving the cell in a state of starvation and triggering lysosomal-, peroxisomal-, and vacuole-autophagy. The autophagosome recycles material from spores, reduces spore production, and induces mycelial swelling, which, in turn, increases lycopene production and storage.</p>","PeriodicalId":14318,"journal":{"name":"International Microbiology","volume":" ","pages":"2115-2127"},"PeriodicalIF":2.3,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144191810","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microbial consortium involving Pseudomonas and Bacillus: strain selection and the effect of co-cultivation on biocontrol activity against phytopathogens and the composition of metabolic extracts.","authors":"João Gabriel Dumont Negrelli, Maira Rafaela de Britto Rafael, Vitor Dib Gazola, Marcus Cesar Bochi Reis Dos Santos, Eduardo Jorge Pilau, Andressa Domingos Polli, Halison Correia Golias, Tiago Tognolli de Almeida, Julio Cesar Polonio","doi":"10.1007/s10123-025-00668-1","DOIUrl":"10.1007/s10123-025-00668-1","url":null,"abstract":"<p><p>Agricultural sustainability is vital to meet the growing global demand for food; therefore, the search for more sustainable options to replace traditional chemical products has gained attention due to their benefits. The sector has applied innovative microbial consortium approaches as a niche for exploring new bioproducts and metabolic pathways through microbial interactions. Thus, this study sought to select two endophytic bacterial strains with biocontrol activity to study their metabolic interactions in culture. For this, co-cultivation and axenic culture assays were carried out to evaluate the inhibition of Corynespora cassiicola, Sclerotinia sclerotiorum, Moniliophthora perniciosa, and Colletotrichum truncatum. After the production of antiphytopathogenic compound tests, two strains were selected: P. putida MG36 and B. amyloliquefaciens SS14. These bacteria were cultivated under three distinct conditions: axenic cultivation of SS14, axenic cultivation of MG36, and co-cultivation. The metabolites were extracted and analyzed by liquid chromatography coupled with mass spectrometry (UHPLC-MS/MS). The results showed that both strains exhibited significant antifungal activity. B. amyloliquefaciens SS14 demonstrated 65% inhibition against C. truncatum, while P. putida MG36 showed 58% inhibition against S. sclerotiorum. Analysis of the chemical profiles revealed the presence of exclusive and shared metabolites, such as iturin A4 (antifungal lipopeptide) and macrolactin A (bioactive polyketide), under different culture conditions. In conclusion, P. putida MG36 and B. amyloliquefaciens SS14 show promise as biocontrol agents against phytopathogens, contributing to more sustainable agricultural practices.</p>","PeriodicalId":14318,"journal":{"name":"International Microbiology","volume":" ","pages":"1935-1947"},"PeriodicalIF":2.3,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144018426","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Saline-alkali resilience: the role of Trametes NF1 in promoting alfalfa growth and salinity tolerance.","authors":"Hao Zou, Ziyue Shi, Jie Liu, Jianan Wang, Ping Li","doi":"10.1007/s10123-025-00680-5","DOIUrl":"10.1007/s10123-025-00680-5","url":null,"abstract":"<p><p>Soil salinization poses a significant threat to agricultural productivity, particularly affecting forage legumes such as alfalfa. Here, we reported the isolation and functional characterization of a novel salt-tolerant fungal strain, Trametes NF1, from a saline-alkali wetland in Tianjin, China, and demonstrated its potential to enhance alfalfa (Medicago sativa L.) growth and salinity tolerance. The strain was identified as belonging to the genus Trametes (phylum Basidiomycota) based on internal transcribed spacer (ITS) gene sequencing. Seed germination and seedling assays revealed that NF1 culture significantly improved alfalfa growth traits, including germination rate, root and bud lengths, stem diameter, leaf number, and fresh weight, with coefficients of determination (R²) ranging from 0.28 to 0.61 (p < 0.01, regression analyses). At a 5% culture concentration, seed germination increased by 34%, and fresh weight by 61%, compared to the control. Biochemical analyses further indicated that NF1 application enhanced peroxidase activity by 201% and soluble protein content by 76%, while reducing malondialdehyde, soluble sugar, proline levels, along with the activity of superoxide dismutase, ascorbate peroxidase, and catalase, suggesting modulations of the antioxidant defense system and osmotic regulation. This study presents the first report of a Trametes strain from saline-alkali soil that promotes alfalfa salt tolerance, providing a novel microbial resource for sustainable management of salinized agroecosystems.</p>","PeriodicalId":14318,"journal":{"name":"International Microbiology","volume":" ","pages":"2151-2160"},"PeriodicalIF":2.3,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144215784","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Screening, isolation, and process optimization of a bioemulsifier-producing Acinetobacter Y-1.","authors":"Hui Wei, Fu-Rong Yao, Ou-Ying Li, Shuo Guo, Jun Li, He-Jing Yan, Feng-Mei Zhu, Jie-Fang Zhou","doi":"10.1007/s10123-025-00664-5","DOIUrl":"10.1007/s10123-025-00664-5","url":null,"abstract":"<p><p>Optimizing the production of bioemulsifiers is critical for sustainable industrial applications. In this study, 27 strains were isolated from activated sludge, and strain Y-1 was identified as the most promising candidate based on emulsification index (EI) and emulsification rating. Strain Y-1 exhibited excellent emulsifying and dispersing activities toward both liquid paraffin and crude oil, with an EI of 65%. Morphological characterization, physiological and biochemical tests, and 16S rDNA sequencing identified Y-1 as a member of the genus Acinetobacter. Fermentation conditions were optimized using single-factor experiments and response surface methodology, and the optimal conditions were determined to be a carbon-to-nitrogen (C/N) ratio of 1:1, inoculum concentration of 2%, and incubation temperature of 34 °C. Under these conditions, the bioemulsifier yield of Acinetobacter Y-1 reached 6.9 g/L, representing a 4.14-fold increase compared to unoptimized conditions. The bioemulsifier produced by Acinetobacter Y-1 effectively emulsified a variety of hydrocarbon substrates, including liquid paraffin, diesel, xylene, olive oil, cutting oil, cold rolling oil, n-hexane, and benzene. Furthermore, it retained stable emulsification activity under extreme conditions, including a wide temperature range (4-80 °C), high salinity (5-30%), and broad pH values (1-14). These findings highlight Acinetobacter Y-1 as a promising strain for the production of high-activity, stable bioemulsifiers with great potential for industrial applications.</p>","PeriodicalId":14318,"journal":{"name":"International Microbiology","volume":" ","pages":"2007-2027"},"PeriodicalIF":2.3,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144078007","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genomic characterization of a novel Pseudomonas aeruginosa bacteriophage representing the newly proposed genus Angoravirus: in vitro antimicrobial and antibiofilm activity.","authors":"Sezin Unlu, Aylin Uskudar Guclu","doi":"10.1007/s10123-025-00669-0","DOIUrl":"10.1007/s10123-025-00669-0","url":null,"abstract":"<p><p>Multi-drug-resistant (MDR) Pseudomonas aeruginosa is an important pathogen that poses a critical threat due to its metallo-beta-lactamase (MBL)-mediated carbapenem resistance and biofilm-forming ability, making bacterial treatment very complicated and requiring alternative strategies. Bacteriophages are promising alternatives; however, the discovery of novel phages targeting MDR strains remains urgent. In this study, Pseudomonas phage Baskent_P4_1, a novel virulent siphovirus that infects clinical MDR P. aeruginosa isolates, was isolated from wastewater and characterized comprehensively. Its efficacy was tested against biofilm-forming, MDR isolates with MBL activity by spot test and efficiency of plating (EOP). Biological characterization showed that phage Baskent_P4_1 is stable at pH 4-10 and temperatures up to 50 °C, while its stability decreases  >60 °C temperature. It has a short latent period of 10 min and a high burst size of 253 phages per cell. The phage lysed 40% of the MDR P. aeruginosa isolates tested, including strong biofilm producers. In vitro assays showed significant biofilm inhibition (48.8% reduction at 10⁹ PFU/mL) and degradation of pre-formed biofilms. Transmission electron microscopy (TEM) revealed an icosahedral head (70 nm) and a long non-contractile tail (150 nm). Whole genome sequencing by Illumina demonstrated a linear dsDNA genome of 41.947 bp (62.8% GC content) with 53 predicted coding sequences. No virulence factors, antibiotic resistance genes, or tRNAs were detected, thus ensuring therapeutic safety. Along with phylogenetic and vConTACT2 analysis, these results suggested that phage Baskent_P4_1 belongs to a new genus, which was proposed here as the genus Angoravirus with three other species. Genomic analysis identified hydrolases (ORF 13/14) and 7-deazaguanine modification enzymes (ORF 46/47) that may contribute to host lysis and evasion of bacterial defenses. These findings highlight Baskent_P4_1's potential as a therapeutic candidate against MDR P. aeruginosa infections. The study underscores the importance of expanding phage diversity libraries and provides a framework for characterizing novel phages to combat antimicrobial resistance.</p>","PeriodicalId":14318,"journal":{"name":"International Microbiology","volume":" ","pages":"1949-1960"},"PeriodicalIF":2.3,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144012022","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction to: Epidemiological and molecular study of Providencia rettgeri outbreak at a university hospital during the COVID‑19 reference center.","authors":"Julia Da Silva Pimenta, Gerusa Luciana Gomes Magalhães, Joao Gabriel Material Soncini, Nilton Lincopan, Eliana Carolina Vespero","doi":"10.1007/s10123-025-00648-5","DOIUrl":"10.1007/s10123-025-00648-5","url":null,"abstract":"","PeriodicalId":14318,"journal":{"name":"International Microbiology","volume":" ","pages":"2271"},"PeriodicalIF":2.3,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143624656","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}