International journal of ophthalmology最新文献

{"title":"Optimized <i>Eleutherine bulbosa</i> Urb. bulb extract on the inhibition of 3D retinoblastoma spheroids cultured in type I murine collagen.","authors":"Ammar Akram Kamarudin, Nor Hafiza Sayuti, Mohamad Zulhafiz Shafiq Zulhilmi Cheng, Norazalina Saad, Nur Hanisah Azmi, Norhaizan Mohd Esa","doi":"10.18240/ijo.2025.05.05","DOIUrl":"10.18240/ijo.2025.05.05","url":null,"abstract":"<p><strong>Aim: </strong>To investigate the efficacy of <i>Eleutherine bulbosa</i> (Mill.) Urb. bulb extract (EBE) on the 3D human retinoblastoma cancer cells (WERI-Rb-1) spheroids and explore its apoptotic mechanism.</p><p><strong>Methods: </strong>The 3D WERI-Rb-1 and human retinal pigmented epithelium cells (ARPE-19) spheroids were developed using type 1 murine collagen that was excised from the rat tail tendon and cultured <i>via</i> hanging drop and embedded techniques. The cytotoxic activity was examined by Alamar blue assay meanwhile, the morphological characteristics were assessed by 4',6-diamidino-2-phenylindole (DAPI) and scanning electron microscopy (SEM). The mRNA and protein expressions of apoptotic and antioxidant signal transduction pathways were explored to ascertain its molecular mechanisms. The statistical analysis was carried out using GraphPad Prism.</p><p><strong>Results: </strong>The Alamar blue assay portrayed higher half maximal inhibitory concentration (IC₅₀) values of EBE and cisplatin on 3D WERI-Rb-1 model as compared to the previous study on 2D model. The results of DAPI and SEM illustrated apoptotic features upon treatment with EBE and cisplatin in a dose-dependent manner on 3D WERI-Rb-1 model. The mRNA and protein levels of apoptotic and antioxidant-related pathways were significantly affected by EBE and cisplatin, respectively (<i>P</i><0.05). The regulation of gene and protein expressions of 3D WERI-Rb-1 spheroids differed from the 2D study, suggesting that the tumor microenvironment of extracellular matrix (ECM) collagen matrix hindered the EBE treatment efficacy, leading to apoptotic evasion.</p><p><strong>Conclusion: </strong>A significant inhibition effect of EBE is observed on the 3D WERI-Rb-1 spheroids. The presence of ECM causes an increase in cytotoxic resistance upon treatment with EBE and cisplatin.</p>","PeriodicalId":14312,"journal":{"name":"International journal of ophthalmology","volume":"18 5","pages":"802-812"},"PeriodicalIF":1.9,"publicationDate":"2025-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12043305/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144093505","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Early assessment of visual outcomes and corneal stability in eyes with a pre-planned residual stromal thickness of 280 to 300 µm following small incision lenticule extraction.","authors":"Yin Liu, Jian-Min Shang, Cheng Hu, Xiao Chen, Wen-Shan Jiang, Jia Huang","doi":"10.18240/ijo.2025.05.16","DOIUrl":"10.18240/ijo.2025.05.16","url":null,"abstract":"<p><strong>Aim: </strong>To assess early visual outcomes and corneal stability following small incision lenticule extraction (SMILE) in eyes with a pre-planned residual stromal thickness (RST) ranging from 280 to 300 µm.</p><p><strong>Methods: </strong>This retrospective study was designed to evaluate 82 eyes from 82 patients, all of whom had a pre-planned RST of 280 to 300 µm and normal corneal topography prior to undergoing SMILE surgery. The mean preoperative spherical equivalent (SE) was -4.82±1.30 D. A standard follow-up protocol was conducted between 1 to 6mo postoperatively. Visual outcomes were recorded using uncorrected visual acuity (UCVA) and subjective refraction. The curvature of the anterior and posterior corneal surfaces, as well as the posterior elevation at the thinnest point (PTE) were derived from the Pentacam system.</p><p><strong>Results: </strong>At the final follow-up, the efficacy index was 1.14±0.15, the safety index was 1.20±0.13. The mean preoperative UDVA was 0.78±0.16 logMAR, which improved significantly to -0.07±0.06 logMAR postoperatively (<i>P</i><0.001). The preoperative mean SE was -4.82±1.30 D, which decreased to -0.14±0.30 D by the last visit. The curvature of the anterior cornea at the flat meridian (AK1) were 42.62±1.02 D preoperatively, 38.56±1.37 D and 38.59±1.39 D at 1 and 6mo after operation, respectively. Corresponding measurements at the steep meridian (AK2) were 43.55±1.14 D preoperatively, 39.18±1.46 D and 39.22±1.50 D at 1 and 6mo after operation, respectively. Both AK1 and AK2 remained stable at 1 and 6-mo postoperative intervals (<i>P</i>=0.126 and 0.082, respectively). There were no observed changes in the curvature of the posterior cornea at the flat meridian or at the steep meridian, or the PTE before and after surgery.</p><p><strong>Conclusion: </strong>SMILE represents a safe and effective procedure for the correction of myopia and astigmatism in eyes featuring a pre-planned RST ranging from 280 to 300 µm accompanied by normal corneal topography, on the premise of strict control of surgical indications.</p>","PeriodicalId":14312,"journal":{"name":"International journal of ophthalmology","volume":"18 5","pages":"897-903"},"PeriodicalIF":1.9,"publicationDate":"2025-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12043307/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144093762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Posterior chamber phakic intraocular lens adjustment-causes and complications: a retrospective cohort study.","authors":"Ghufran Alarfaj, Halah Bin Helayel, Majed AlSubaie, Jumana Hariri, Fatima Alzaher, Omar Khan, Mohanna Al-Jindan, Ahmed AlHabash, Naif M Sulaimani","doi":"10.18240/ijo.2025.05.14","DOIUrl":"10.18240/ijo.2025.05.14","url":null,"abstract":"<p><strong>Aim: </strong>To evaluate the visual outcomes of implantable collamer lenses (ICLs) and identify the possible risk factors for ICL axis misalignment, and consequently, repositioning, explanting, or exchanging at a specialized eye hospital in Saudi Arabia.</p><p><strong>Methods: </strong>The medical records of 813 eyes with different refractive errors corrected with ICL implantation were identified and included in this single-arm retrospective cohort study. The following data were collected: demographic characteristics, primary diagnosis, preoperative refraction, anterior chamber depth (ACD), white-to-white (WTW) measurement, endothelial cell density (ECD), and axial length. Patients' satisfaction and complaints, and their postoperative refraction, vault depth, and axis alignment with the preoperative target, were reviewed during the postoperative period. Collectively, these data were correlated with symptomatic axis rotation and the need for repositioning, explantation, or exchange due to high or low ICL vaults.</p><p><strong>Results: </strong>Of 813 eyes, 27 (3.32%), 13 (1.59%), and 11 (1.35%) required ICL repositioning, ICL explantation only without exchange, and ICL explantation with the placement of a new ICL, respectively. The mean follow-up period was 37.5mo. The main cause of explanation or exchange was incorrect WTW measurement in seven (29.17%) eyes, followed by high vault in four (16.56%) eyes. ICL repositioning was required in 27 (3.32%) eyes with considerable rotation. Only 2 (0.24%) eyes developed cataracts that required ICL removal, and retinal complications were reported in 7 (0.86%) eyes. Long-term glaucoma and corneal decompensation were not observed in this cohort.</p><p><strong>Conclusion: </strong>With a high safety profile and reversibility, ICL implantation is a good alternative to corneal-based refractive surgery in eyes unsuitable for laser vision correction. The rate of secondary procedures in our study was 6.26%. Old age is a risk factor for secondary surgical interventions in the repositioning group, whereas abnormal vault and toric ICL rotation in the explantation group necessitated subsequent surgical procedures. Overall, ICL implantation demonstrates a good efficacy index and safety profile in patients with diverse refractive errors.</p>","PeriodicalId":14312,"journal":{"name":"International journal of ophthalmology","volume":"18 5","pages":"883-888"},"PeriodicalIF":1.9,"publicationDate":"2025-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12043295/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144093596","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tracing global progress: two decades of age-related macular degeneration research.","authors":"Li-Yun Yuan, Liang-Pin Li, Xia Hua, Xiao-Yong Yuan","doi":"10.18240/ijo.2025.05.20","DOIUrl":"10.18240/ijo.2025.05.20","url":null,"abstract":"<p><strong>Aim: </strong>To conduct a comprehensive bibliometric analysis of age-related macular degeneration (AMD) research from 2002 to 2022, identifying key contributing countries, institutions, authors, journals, and research hotspots to inform future research directions.</p><p><strong>Methods: </strong>Publications related to AMD were retrieved from the Web of Science Core Collection (WoSCC) database for the period January 1, 2002, to December 31, 2022. The search was limited to English-language articles and reviews. Bibliometric analysis was performed using Microsoft Excel 2021 for data management and annual publication analysis. Visualization and network analyses were conducted using VOSviewer, CiteSpace, and the Bibliometrix package in R. Collaboration networks among countries, institutions, authors, and journals were mapped. Keywords were analyzed for co-occurrence to identify research hotspots. Metrics such as H-index, total link strength (TLS), and citation counts were used to assess impact.</p><p><strong>Results: </strong>A total of 16 715 publications were analyzed, showing a consistent increase in AMD research output over the past 20y, peaking at 1445 publications in 2021. The United States was the leading contributor with 31.8% of total publications, followed by China and the United Kingdom. The University of Melbourne emerged as the most productive institution with the highest TLS, indicating strong international collaborations. Professor Frank G. Holz was identified as the most influential author based on H-index and publication count. <i>Investigative Ophthalmology & Visual Science</i> was the most prolific journal and had the highest citation impact. Keyword co-occurrence analysis revealed four main research clusters: pathogenesis, therapy, epidemiology, and diagnosis. Emerging research hotspots included anti-vascular endothelial growth factor (VEGF) therapies, optical coherence tomography angiography, and artificial intelligence (AI) applications in diagnosis.</p><p><strong>Conclusion: </strong>The bibliometric analysis highlights significant growth and collaborative efforts in AMD research globally. Key contributors have advanced understanding in pathogenesis, therapeutic strategies, epidemiology, and diagnostic technologies. Future research should focus on interdisciplinary collaborations, novel therapeutic targets, personalized medicine, and technological innovations such as AI to effectively address the challenges posed by AMD.</p>","PeriodicalId":14312,"journal":{"name":"International journal of ophthalmology","volume":"18 5","pages":"925-936"},"PeriodicalIF":1.9,"publicationDate":"2025-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12043290/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144093608","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nintedanib regulates miR-23b-3p/TGFBR2 axis and competitively binds to TGFBR2 protein, inhibiting EMT process in human pterygium cells.","authors":"Ke-Ke Zhang, Meng Li, Yan-Hong Liao, Xiao-Tian Liu, Yong-Bo Bao, Yan Gong","doi":"10.18240/ijo.2025.05.03","DOIUrl":"10.18240/ijo.2025.05.03","url":null,"abstract":"<p><strong>Aim: </strong>To investigate the effects of nintedanib on epithelial-mesenchymal transition (EMT) in cells derived from pterygium, aiming to explore its potential as a pharmacological intervention for pterygium treatment.</p><p><strong>Methods: </strong>Primary human pterygium epithelial cells (hPEC) and human conjunctival epithelial (hCJE) cells were isolated from patients, cultured, and characterized. The impact of nintedanib on transforming growth factor beta (TGF-β)-induced EMT was assessed by examining the expression of EMT markers such as vimentin and E-cadherin. Additionally, the modulation of the miR-23b-3p/transforming growth factor beta receptor 2 (TGFBR2)/Smad2 pathway by nintedanib was investigated to elucidate its potential antifibrotic mechanism.</p><p><strong>Results: </strong>The expression of miR-23b-3p gene in hCJE cells was significantly higher than that in hPEC cells. Nintedanib effectively mitigated TGF-β-induced EMT in cells derived from pterygium, as evidenced by the downregulation of vimentin and upregulation of E-cadherin. When the nintedanib concentration exceeded 1 µmol/L, it significantly suppressed the proliferation of hPEC cells and significantly inhibited the migration distance of hPEC cells within 48h (<i>P</i><0.01). The immunoprecipitation experiment showed that nintedanib modulated the TGFBR2 protein's response to TGF-β independently of miR-23b-3p. Both nintedanib and transfection with miR-23b-3p mimic significantly inhibited the expression levels of phosphorylated Smad2, snail homolog 1 (Drosophila, SNAIL), and SNAI2 (also known as SLUG, snail family transcriptional repressor 2) proteins.</p><p><strong>Conclusion: </strong>Nintedanib is found to modulate the miR-23b-3p/TGFBR2/Smad2 pathway, suggesting a novel antifibrotic mechanism. These findings collectively highlight nintedanib's therapeutic potential in managing pterygium, marking a significant step toward non-surgical treatment options. Nintedanib may offer a targeted pharmacological treatment that could complement or reduce the need for surgical interventions.</p>","PeriodicalId":14312,"journal":{"name":"International journal of ophthalmology","volume":"18 5","pages":"779-791"},"PeriodicalIF":1.9,"publicationDate":"2025-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12043287/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144093491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An improved technique to treat persistent extensive Descemet's membrane detachment following cataract surgery: a case report.","authors":"Ru-Xin Gao, Rong Zhang, Yan Wang, Ying-Feng Hu, Xiang-Yu Ye","doi":"10.18240/ijo.2025.05.23","DOIUrl":"10.18240/ijo.2025.05.23","url":null,"abstract":"","PeriodicalId":14312,"journal":{"name":"International journal of ophthalmology","volume":"18 5","pages":"958-961"},"PeriodicalIF":1.9,"publicationDate":"2025-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12043309/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144093699","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Guidelines for preoperative visual function and imaging examination standards in vitreoretinal surgery (2025).","authors":"Yi Shao, Jian Ma, Zhao-Yang Wang","doi":"10.18240/ijo.2025.05.06","DOIUrl":"10.18240/ijo.2025.05.06","url":null,"abstract":"<p><p>This guideline seeks to thoroughly investigate the standardized operational procedures for visual function and imaging examinations prior to vitreoretinal surgery. Preoperative assessments can greatly assist clinicians in determining surgical indications, assessing patient conditions, and offering valuable assistance in formulating surgical strategies and predicting outcomes. Developed by a collaborative team of experts from the Ophthalmic Imaging and Intelligent Medicine Branch of the Chinese Medical Education Association, in conjunction with the Ophthalmic Imaging and Intelligent Medicine Branch of the Chinese Medical Education Association, these guidelines have been formulated through extensive research and evaluation, incorporating the latest technological advancements and studies on a global and domestic scale in vitreoretinal surgery. After extensive deliberations and incorporation of up-to-date clinical data, these guidelines have been developed to assist in standardizing preoperative examinations for vitreoretinal surgery. The overarching goals include improving medical quality, maximizing resource allocation, offering decision-making assistance, and safeguarding patient rights. This document provides a comprehensive analysis of preoperative assessments for vitreoretinal procedures, covering principles, methodologies, and precautions related to a range of diagnostic techniques including ultra-wide-angle fundus imaging, fluorescein angiography, indocyanine green angiography, ophthalmic B-ultrasound examinations, ultrasound biomicroscopy, optical coherence tomography, optical coherence tomography angiography, orbital CT scan, orbital MRI, and ophthalmic electrophysiology tests such as electroretinograms, visually evoked potentials, and visual field testing.</p>","PeriodicalId":14312,"journal":{"name":"International journal of ophthalmology","volume":"18 5","pages":"813-831"},"PeriodicalIF":1.9,"publicationDate":"2025-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12043299/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144093765","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Interventions in functional epiphora-a systematic review.","authors":"Evelyn Li Min Tai, Allan Lu Lee, Yee Cheng Kueh, Baharudin Abdullah, Bernard Chang","doi":"10.18240/ijo.2025.05.21","DOIUrl":"10.18240/ijo.2025.05.21","url":null,"abstract":"<p><strong>Aim: </strong>To review the success rates and complications of interventions for functional epiphora in adults.</p><p><strong>Methods: </strong>A systematic review of English-language articles from the electronic databases PubMed, SCOPUS, and Google Scholar. The primary outcome was subjective resolution or improvement of epiphora symptoms. Secondary outcomes were treatment-related adverse events. Subjects above 18 years of age who underwent surgical or non-surgical treatment for functional epiphora (exhibited symptoms of epiphora with a patent lacrimal system) were included. Articles were excluded if they were 1) case reports; 2) abstract only studies; 3) published in a language other than English. Data extraction was performed independently by two authors. The Effective Public Health Practice Project checklist was used for quality assessment of the included studies.</p><p><strong>Results: </strong>A total of 762 articles were identified; 28 met the study criteria. Most studies employed silicone tube intubation alone or as an adjuvant procedure to dacryocystorhinostomy (DCR). Other interventions included lacrimal probing, balloon dacryoplasty, lateral tarsal strip and botulinum toxin A. DCR had the highest success rate, as well as the longest mean follow-up time. Complications were minor, transient, and mostly stent-related.</p><p><strong>Conclusion: </strong>This updated systematic review on the success rates of interventions for functional epiphora in adults proposes the following management algorithm. Dacryocystography (DCG) should be performed in all patients with functional epiphora. If DCG is abnormal, we advocate DCR. If DCG is normal, proceed with dacryoscintigraphy (DSG). We perform DCR for post-sac delay on DSG and lateral tarsal strip for pre-sac delay. Botulinum toxin A is an off-label, short-term treatment option in those with normal DSG.</p>","PeriodicalId":14312,"journal":{"name":"International journal of ophthalmology","volume":"18 5","pages":"937-950"},"PeriodicalIF":1.9,"publicationDate":"2025-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12043291/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144093392","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Application of smartphone ophthalmoscope in ophthalmic clinical practice and teaching.","authors":"Jia-Ning Qiu, Jing Luo, Man-Yun Xie","doi":"10.18240/ijo.2025.05.22","DOIUrl":"10.18240/ijo.2025.05.22","url":null,"abstract":"<p><p>The rapid development and increasing popularity of smartphones have led to their gradual integration as essential aids in medical examinations and teaching tools. The smartphone ophthalmoscope (SO) is one of them. Specifically, SO is comprised of a smartphone and an attachment. The smartphone serves as a versatile device for recording and transmitting information, while the attachment functions as a tool for phone fixation as well as focus adjustment and providing light resources, albeit with slight variations across different devices. Presently, the convenience, universality, and transferability of SO have greatly expanded its potential in fields of clinical practice and ocular teaching. The review provides a concise overview of the contemporary SO devices, elucidates the diseases amenable to assessment <i>via</i> SO, and outlines the various applications of SO in clinical practice and teaching.</p>","PeriodicalId":14312,"journal":{"name":"International journal of ophthalmology","volume":"18 5","pages":"951-957"},"PeriodicalIF":1.9,"publicationDate":"2025-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12043301/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144093724","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prognostic prediction model for Chinese uveal melanoma patients based on matrix metalloproteinase-2 and -28 expression levels in the tumor.","authors":"Yu-Ning Chen, Jing-Ying Xiu, Han-Qing Zhao, Jing-Ting Luo, Qiong Yang, Yang Li, Wen-Bin Wei","doi":"10.18240/ijo.2025.05.02","DOIUrl":"10.18240/ijo.2025.05.02","url":null,"abstract":"<p><strong>Aim: </strong>To explore the relationship between matrix metalloproteinases (MMPs) expression levels in the tumor and the prognosis of uveal melanoma (UM) and to construct prognostic prediction models.</p><p><strong>Methods: </strong>Transcriptome sequencing data from 17 normal choroid tissues and 53 UM tumor tissues were collected. Based on the differential gene expression levels and their function, MMPs family was selected for establishing risk-score system and prognostic prediction model with machine learning. Tumor microenvironment (TME) analysis was also applied for the impact of immune cell infiltration on prognosis of the disease.</p><p><strong>Results: </strong>Eight MMPs were significantly different expression levels between normal and the tumor tissues. MMP-2 and MMP-28 were selected to construct a risk-score system and divided patients accordingly into high- and low-risk groups. The prediction model based on the risk-score achieved an accuracy of approximately 80% at 1-, 3-, and 5-year after diagnosis. Besides, a Nomogram prognostic prediction model which based on risk-score and pathological type (independent prognostic factors after Cox regression analysis) demonstrated good consistency between the predicted outcomes at 1-, 3-, and 5-year after diagnosis and the actual prognosis of patients. TME analysis revealed that the high-risk group exhibited higher immune and stromal scores and increased infiltration of tumor-associated macrophages (TAMs) and regulatory T cells compared to the low-risk group.</p><p><strong>Conclusion: </strong>Based on MMP-2 and MMP-28 expression levels, our prediction model demonstrates accurate long-term prognosis prediction for UM patients. The aggregation of TAMs and regulatory T cells in the TME of UM may be associated with an unfavorable prognosis.</p>","PeriodicalId":14312,"journal":{"name":"International journal of ophthalmology","volume":"18 5","pages":"765-778"},"PeriodicalIF":1.9,"publicationDate":"2025-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12043293/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144093600","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}