International Journal of Experimental Pathology最新文献_第8页

MicroRNA-23a-3p promotes macrophage M1 polarization and aggravates lipopolysaccharide-induced acute lung injury by regulating PLK1/STAT1/STAT3 signalling MicroRNA-23a-3p通过调节PLK1/STAT1/STAT3信号传导促进巨噬细胞M1极化并加重脂多糖诱导的急性肺损伤。

IF 3 4区医学

International Journal of Experimental Pathology Pub Date : 2022-06-23 DOI: 10.1111/iep.12445

Tao Jiang, Li Sun, Jun Zhu, Ning Li, Haibo Gu, Ying Zhang, Miaomiao Li, Jiayao Xu

引用次数: 4

Pre-analytical processing protocol of breast biopsies affects multigene panel results 乳腺活检的前分析处理方案影响多基因小组结果

IF 3 4区医学

International Journal of Experimental Pathology Pub Date : 2022-05-15 DOI: 10.1111/iep.12444

Vanessa Reinaldo Lima, Beatriz da Costa Aguiar Alves, Fernando Luiz Affonso Fonseca, Debora Krutman Zveibil, Auro del Giglio

{"title":"Pre-analytical processing protocol of breast biopsies affects multigene panel results","authors":"Vanessa Reinaldo Lima, Beatriz da Costa Aguiar Alves, Fernando Luiz Affonso Fonseca, Debora Krutman Zveibil, Auro del Giglio","doi":"10.1111/iep.12444","DOIUrl":"10.1111/iep.12444","url":null,"abstract":"The creation of multigene panels for prognostic and predictive purposes allows a more accurate indication of adjuvant chemotherapy for patients with breast cancer. In a previous study, we reproduced a multigene panel of 21 genes based on the commercial Oncotype-DX method. We submitted 183 embedded specimens obtained from breast surgery on patients with locoregional disease (stages I to III) between 2005 and 2010 performed at the Hospitals of the Medical School of the ABC Foundation. When we analysed the correlations between the score of the multigene panel and the progression-free interval (PFI) in all patients, we did not find a statistically significant association. However, when we selected only the 71 samples that had amplification of at least eight non-housekeeping genes, we observed that those with scores above the 75th percentile had a significantly lower PFI (p = .0054). Samples processed with nonbuffered formaldehyde were associated with a worse quality of extracted RNA (p = .004) and a significantly higher multigene panel score (p = .021). We conclude that variations in the pre-analytical processing of specimens destined for multigene panel amplification can significantly affect the results, with a potential impact on clinical management.","PeriodicalId":14157,"journal":{"name":"International Journal of Experimental Pathology","volume":"103 3","pages":"112-120"},"PeriodicalIF":3.0,"publicationDate":"2022-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9556869","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expression of airway smooth muscle contractile proteins in children with acute interstitial pneumonia 急性间质性肺炎患儿气道平滑肌收缩蛋白的表达。

IF 3 4区医学

International Journal of Experimental Pathology Pub Date : 2022-05-08 DOI: 10.1111/iep.12443

Fang Cheng, Tao Lu, Yicheng Wang, Didi Yuan, Zehong Wei, Yongguo Li, Jianbo Li, Renkuan Tang

{"title":"Expression of airway smooth muscle contractile proteins in children with acute interstitial pneumonia","authors":"Fang Cheng, Tao Lu, Yicheng Wang, Didi Yuan, Zehong Wei, Yongguo Li, Jianbo Li, Renkuan Tang","doi":"10.1111/iep.12443","DOIUrl":"10.1111/iep.12443","url":null,"abstract":"The purpose of the present study was to investigate the expression of α-SMA and SM22α in airway smooth muscle (ASM) of bronchioles from children younger than 14 years who died of acute interstitial pneumonia (AIP). This is based upon the hypothesis that as contractile marker proteins α-SMA and SM22α can serve as an index of the overcontractile phenotype of ASM that is seen in AIP. Lung tissue samples of children were obtained from autopsies and divided into the AIP group (55.9% male and 44.1% female, between 0.4 and 132 months old, n = 34) and the control group (60% male and 40% female, between 2 and 156 months old, n = 10). We recorded the post-mortem interval (PMI), height, clinical symptoms and abdominal fat thickness (AFT) of each case. Haematoxylin-and-eosin-stained sections were used to examine the luminal area and observe the morphological changes in the bronchioles. Immunohistochemistry and Masson's trichrome staining were used to detect the expression of contractile marker proteins and the degree of pulmonary fibrosis respectively. Compared with the control group, the luminal areas of bronchioles in the AIP group were smaller (p < .001). The expression differences in α-SMA and SM22α between the two groups were statistically significant (p = .01 and p = .02 respectively). Also, there was no significant correlation of the contractile marker proteins expression with PMI, height, clinical symptoms and AFT. The collagen deposition difference in lung between the two groups was not statistically significant (p = .224). These findings suggest that enhancement of ASM contractile function appears to be involved in the death mechanism of children with AIP, which affords more insights into the understanding of AIP.","PeriodicalId":14157,"journal":{"name":"International Journal of Experimental Pathology","volume":"103 5","pages":"190-197"},"PeriodicalIF":3.0,"publicationDate":"2022-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41141076","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparison of topical sucralfate with dexpanthenol in rat wound model 外用硫糖钠与葡聚糖醇在大鼠创伤模型中的比较

IF 3 4区医学

International Journal of Experimental Pathology Pub Date : 2022-04-20 DOI: 10.1111/iep.12441

Eda Yildizhan, Burak Veli Ulger, Murat Akkus, Dilara Akinci, Omer Basol

{"title":"Comparison of topical sucralfate with dexpanthenol in rat wound model","authors":"Eda Yildizhan, Burak Veli Ulger, Murat Akkus, Dilara Akinci, Omer Basol","doi":"10.1111/iep.12441","DOIUrl":"10.1111/iep.12441","url":null,"abstract":"Wound healing is a dynamic process initiated in response to injury. There are many factors that have detrimental effects on the wound healing process. Numerous studies have been conducted for improving wound healing processes. Dexpanthenol is widely used to accelerate wound healing. Sucralfate is used for the treatment of peptic ulcers. We aimed to compare the efficacy of topical Dexpanthenol and Sucralfate in an experimental wound model in rats via histopathological examinations and immune histochemical determinations, as well, to evaluate their effects on EGF levels. Three different groups were formed: the Control Group, the Dexpanthenol Group and the Sucralfate Group. Full-thickness skin wounds were created on the back of each rat and isotonic saline was applied to the wounds of the rats in the control group, Bepanthol® cream was applied in Dexpanthenol Group and 10% Sucralfate cream was applied in Sucralfate Group, once a day. On the 7th, 14th and 21st days the wounds were measured and seven rats from each group were sacrificed and the wounds were excised for histopathological examination. Sucralfate increased wound healing rates by increasing neovascularization, fibroblast activation, reepithelialization and collagen density, as well as dexpanthenol. Our study revealed that the dexpanthenol and sucralfate groups were better than the control group in terms of their effects on wound healing, however there was no statistically significant difference among these two groups. Sucralfate improves EGF expression in skin wounds and has positive results on skin wound healing comparable to dexpanthenol.","PeriodicalId":14157,"journal":{"name":"International Journal of Experimental Pathology","volume":"103 4","pages":"164-170"},"PeriodicalIF":3.0,"publicationDate":"2022-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9910390","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Correlations between histological characterizations and methylation statuses of tumour suppressor genes in Wilms' tumours Wilms肿瘤中肿瘤抑制基因的组织学特征与甲基化状态之间的相关性

IF 3 4区医学

International Journal of Experimental Pathology Pub Date : 2022-04-18 DOI: 10.1111/iep.12442

Yen-Chein Lai, Meng-Yao Lu, Wen-Chung Wang, Tai-Cheng Hou, Chen-Yun Kuo

{"title":"Correlations between histological characterizations and methylation statuses of tumour suppressor genes in Wilms' tumours","authors":"Yen-Chein Lai, Meng-Yao Lu, Wen-Chung Wang, Tai-Cheng Hou, Chen-Yun Kuo","doi":"10.1111/iep.12442","DOIUrl":"10.1111/iep.12442","url":null,"abstract":"Wilms' tumour is a solid tumour that frequently occurs in children. Genetic changes in WT1 and epigenetic aberrations that affect imprinted control region 1 in WT2 loci are implicated in its aetiology. Moreover, tumour suppressor genes are frequently silenced by methylation in this tumour. In the present study, we analysed the methylation statuses of promoter regions of 24 tumour suppressor genes using a methylation-specific multiplex ligation-dependent probe amplification (MS-MLPA)-based approach in 6 Wilms' tumours. Methylation of RASSF1 was specific to all 6 Wilms' tumours and was not observed in normal tissues. Moreover, methylated HIC1 was identified in stromal-type Wilms' tumours and methylated BRCA1 was identified in epithelial-type Wilms' tumours. Unmethylated CASP8, RARB, MLH1_167, APC and CDKN2A were found only in blastemal predominant-type Wilms' tumour. Our results indicated that methylation of RASSF1 may be a vital event in the tumorigenesis of Wilms' tumour, which informs its clinical and therapeutic management. In addition, mixed-type Wilms' tumours may be classified according to epithelial, stromal and blastemal components via MS-MLPA-based approach.","PeriodicalId":14157,"journal":{"name":"International Journal of Experimental Pathology","volume":"103 3","pages":"121-128"},"PeriodicalIF":3.0,"publicationDate":"2022-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9548330","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

lncRNA MIAT targets miR-411-5p/STAT3/PD-L1 axis mediating hepatocellular carcinoma immune response lncRNA MIAT靶向miR-411-5p/STAT3/PD-L1轴介导肝癌免疫应答

IF 3 4区医学

International Journal of Experimental Pathology Pub Date : 2022-04-15 DOI: 10.1111/iep.12440

Xiaoxia Zhang, Banglun Pan, Jiacheng Qiu, Xiaoling Ke, Shuling Shen, Xiaoqian Wang, Nanhong Tang

{"title":"lncRNA MIAT targets miR-411-5p/STAT3/PD-L1 axis mediating hepatocellular carcinoma immune response","authors":"Xiaoxia Zhang, Banglun Pan, Jiacheng Qiu, Xiaoling Ke, Shuling Shen, Xiaoqian Wang, Nanhong Tang","doi":"10.1111/iep.12440","DOIUrl":"10.1111/iep.12440","url":null,"abstract":"Emerging evidences have shown that long noncoding RNA (lncRNA) plays an important role in the immune escape of cancer cells. Our previous study has demonstrated that lncRNA MIAT is associated with the immune infiltration of hepatocellular carcinoma (HCC). However, the underlying mechanism of MIAT regulating the PD-L1-mediated immune escape of HCC is poorly understood. Quantitative real-time PCR (qRT-PCR) was used to detect the expression of MIAT and PD-L1 mRNA in HCC. The relationship between MIAT, miR-411-5p, STAT3 and PD-L1 was explored by dual-luciferase reporter assay, cytotoxicity assay, Western blot and RNA immunoprecipitation (RIP). In addition, the xenograft model was established to determine the effect of MIAT on PD-L1 expression in vivo. We found that MIAT and PD-L1 were significantly upregulated in HCC tissues and the expression of PD-L1 was regulated by MIAT. The knockdown of MIAT enhanced the cytotoxicity of T cells on HCC cells. MIAT negatively regulated miR-411-5p expression, upregulated STAT3 and ultimately increased PD-L1 expression from the transcription level. The inhibition of miR-411-5p reversed STAT3 and PD-L1 expression inhibited by MIAT knockdown in HCC cells. This study suggests a novel lncRNA-mediated mechanism for HCC cells to evade the immune response; MIAT/miR-411-5p/STAT3/PD-L1 may be a novel therapeutic target for HCC.","PeriodicalId":14157,"journal":{"name":"International Journal of Experimental Pathology","volume":"103 3","pages":"102-111"},"PeriodicalIF":3.0,"publicationDate":"2022-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9559826","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

High level of FHL2 exacerbates the outcome of non-small cell lung cancer (NSCLC) patients and the malignant phenotype in NSCLC cells 高水平的FHL2加剧了非小细胞肺癌(NSCLC)患者的预后和NSCLC细胞的恶性表型

IF 3 4区医学

International Journal of Experimental Pathology Pub Date : 2022-04-02 DOI: 10.1111/iep.12436

Na Li, Ling Xu, Ji Zhang, Yongyu Liu

{"title":"High level of FHL2 exacerbates the outcome of non-small cell lung cancer (NSCLC) patients and the malignant phenotype in NSCLC cells","authors":"Na Li, Ling Xu, Ji Zhang, Yongyu Liu","doi":"10.1111/iep.12436","DOIUrl":"10.1111/iep.12436","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 Non-small cell lung cancer (NSCLC) is a malignant tumour with high mortality. FHL2 has been identified as a biomarker of lung cancer. This research explored the effects of FHL2 expression on NSCLC. NSCLC-associated data sets were collected from the assistant for clinical bioinformatics and TCGA databases respectively. The association between FHL2 and clinical characteristics, the prognostic significance of FHL2 and the influences of various variables on NSCLC were determined by Pearson's chi-squared test, the Kaplan–Meier curve and the Cox regression model respectively. FHL2 level was altered by cell transfection and was measured by qRT-PCR. Tumour xenograft formation was completed by inoculating sh-FHL2/pcDNA-FHL2 transfected cells into BALB/c nude mice. Protein expression was assessed by western blot. Cell apoptosis, proliferation and epithelial - mesenchymal transition (EMT) characteristics were evaluated employing TUNEL, BrdU+ and microscopic observation respectively. The expression of Ki67 and N-cadherin was assessed by immunohistochemistry. The results showed that FHL2 was highly expressed in NSCLC tissues. Patients with high FHL2 expression experienced lower overall survival probability. FHL2 knockdown promoted apoptosis, but inhibited EMT of A549 and NCI-H460 cells, which was verified by the increased ratios of cleaved caspase 9/caspase 9 and cleaved caspase 3/caspase 3, as well as augmented E-cadherin and reduced N-cadherin. In an in vivo assay FHL2 knockdown decreased tumour volume and weight, repressed EMT, but enhanced apoptosis. FHL2 upregulation showed the opposite effects of FHL2 knockdown. Furthermore, FHL2 upregulation facilitated cell proliferation both in in vitro and in vivo assays. These outcomes indicated that high level of FHL2 facilitated tumorigenesis, as well as the proliferation and EMT of NSCLC cells.\u0000 </section>\u0000 </div>","PeriodicalId":14157,"journal":{"name":"International Journal of Experimental Pathology","volume":"103 3","pages":"90-101"},"PeriodicalIF":3.0,"publicationDate":"2022-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9556385","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Transient atrial inflammation in a murine model of Coxsackievirus B3‐induced myocarditis 柯萨奇病毒B3诱导的心肌炎小鼠模型中一过性心房炎症

IF 3 4区医学

International Journal of Experimental Pathology Pub Date : 2022-04-01 DOI: 10.1111/iep.12438

Ling-Fei Wu, M. D. Fiet, Daan R Raaijmakers, L. Woudstra, A. V. van Rossum, H. Niessen, P. Krijnen

{"title":"Transient atrial inflammation in a murine model of Coxsackievirus B3‐induced myocarditis","authors":"Ling-Fei Wu, M. D. Fiet, Daan R Raaijmakers, L. Woudstra, A. V. van Rossum, H. Niessen, P. Krijnen","doi":"10.1111/iep.12438","DOIUrl":"https://doi.org/10.1111/iep.12438","url":null,"abstract":"Atrial dysfunction is a relatively common complication of acute myocarditis, although its pathophysiology is unclear. There is limited information on myocarditis‐associated histological changes in the atria and how they develop in time. The aim of this study therefore was to investigate inflammation, fibrosis and viral genome in the atria in time after mild CVB3‐induced viral myocarditis (VM) in mice. C3H mice (n = 68) were infected with 105 PFU of Coxsackievirus B3 (CVB3) and were compared with uninfected mice (n = 10). Atrial tissue was obtained at days 4, 7, 10, 21, 35 or 49 post‐infection. Cellular infiltration of CD45+ lymphocytes, MAC3+ macrophages, Ly6G+ neutrophils and mast cells was quantified by (immuno)histochemical staining. The CVB3 RNA was determined by in situ hybridization, and fibrosis was evaluated by elastic van Gieson (EvG) staining. In the atria of VM mice, the numbers of lymphocytes on days 4 and 7 (p < .05) and days 10 (p < .01); macrophages on days 7 (p < .01) and 10 (p < .05); neutrophils on days 4 (p < .05); and mast cells on days 4 and 7 (p < .05) increased significantly compared with control mice and decreased thereafter to basal levels. No cardiomyocyte death was observed, and the CVB3 genome was detected in only one infected mouse on Day 4 post‐infection. No significant changes in the amount of atrial fibrosis were found between VM and control mice. A temporary increase in inflammation is induced in the atria in the acute phase of CVB3‐induced mild VM, which may facilitate the development of atrial arrhythmia and contractile dysfunction.","PeriodicalId":14157,"journal":{"name":"International Journal of Experimental Pathology","volume":"111 1","pages":"149 - 155"},"PeriodicalIF":3.0,"publicationDate":"2022-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77596743","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Transient atrial inflammation in a murine model of Coxsackievirus B3-induced myocarditis 柯萨奇病毒B3诱导的心肌炎小鼠模型中的短暂性心房炎症

IF 3 4区医学

International Journal of Experimental Pathology Pub Date : 2022-04-01 DOI: 10.1111/iep.12438

Linghe Wu, Mitchell D. Fiet, Daan R. Raaijmakers, Linde Woudstra, Albert C. van Rossum, Hans W. M. Niessen, Paul A. J. Krijnen

{"title":"Transient atrial inflammation in a murine model of Coxsackievirus B3-induced myocarditis","authors":"Linghe Wu, Mitchell D. Fiet, Daan R. Raaijmakers, Linde Woudstra, Albert C. van Rossum, Hans W. M. Niessen, Paul A. J. Krijnen","doi":"10.1111/iep.12438","DOIUrl":"https://doi.org/10.1111/iep.12438","url":null,"abstract":"Atrial dysfunction is a relatively common complication of acute myocarditis, although its pathophysiology is unclear. There is limited information on myocarditis-associated histological changes in the atria and how they develop in time. The aim of this study therefore was to investigate inflammation, fibrosis and viral genome in the atria in time after mild CVB3-induced viral myocarditis (VM) in mice. C3H mice (n = 68) were infected with 105 PFU of Coxsackievirus B3 (CVB3) and were compared with uninfected mice (n = 10). Atrial tissue was obtained at days 4, 7, 10, 21, 35 or 49 post-infection. Cellular infiltration of CD45+ lymphocytes, MAC3+ macrophages, Ly6G+ neutrophils and mast cells was quantified by (immuno)histochemical staining. The CVB3 RNA was determined by in situ hybridization, and fibrosis was evaluated by elastic van Gieson (EvG) staining. In the atria of VM mice, the numbers of lymphocytes on days 4 and 7 (p < .05) and days 10 (p < .01); macrophages on days 7 (p < .01) and 10 (p < .05); neutrophils on days 4 (p < .05); and mast cells on days 4 and 7 (p < .05) increased significantly compared with control mice and decreased thereafter to basal levels. No cardiomyocyte death was observed, and the CVB3 genome was detected in only one infected mouse on Day 4 post-infection. No significant changes in the amount of atrial fibrosis were found between VM and control mice. A temporary increase in inflammation is induced in the atria in the acute phase of CVB3-induced mild VM, which may facilitate the development of atrial arrhythmia and contractile dysfunction.","PeriodicalId":14157,"journal":{"name":"International Journal of Experimental Pathology","volume":"103 4","pages":"149-155"},"PeriodicalIF":3.0,"publicationDate":"2022-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/iep.12438","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71912333","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Inflammatory response and immunohistochemical characterization of experimental calcium silicate-based perforation repair material 实验性硅酸钙基穿孔修复材料的炎症反应和免疫组织化学特征

IF 3 4区医学

International Journal of Experimental Pathology Pub Date : 2022-04-01 DOI: 10.1111/iep.12439

Hend Okasha, Ashraf M. Abu-Seida, Ahmed A. Hashem, Salma H. El Ashry, Mohamed M. Nagy

{"title":"Inflammatory response and immunohistochemical characterization of experimental calcium silicate-based perforation repair material","authors":"Hend Okasha, Ashraf M. Abu-Seida, Ahmed A. Hashem, Salma H. El Ashry, Mohamed M. Nagy","doi":"10.1111/iep.12439","DOIUrl":"10.1111/iep.12439","url":null,"abstract":"This study compares the immunohistochemical reaction of a new experimental tricalcium silicate perforation repair material to mineral trioxide aggregate (MTA) and Biodentine. A total of 162 mature premolar teeth from 12 dogs were divided into three experimental groups (n = 54 teeth each) according to the evaluation period: 1, 2 and 3 months. Each group was further divided into two equal subgroups (n = 27 teeth each) according to the time of repair: immediate repair and delayed repair. Each subgroup was subdivided according to the material used into three experimental subdivisions (n = 8 teeth each): MTA, Biodentine (Septodont) and experimental material, and two control subdivisions: positive control (n = 2 teeth) and negative control (one tooth). Under general anaesthesia, access cavity was done. Cleaning and shaping were performed using ProTaper universal rotary instruments. The canals were obturated using cold lateral compaction technique with Gutta percha and Adseal sealer. Furcation perforations were created then randomly sealed using the three materials either immediately or after one month (delayed repair). Inflammatory cell count and immunohistochemical analysis of osteopontin-positive area fraction were digitally analysed using the ImageJ software. Delayed furcal perforation repair showed significantly higher inflammatory cell count than immediate repair. No significant difference in inflammatory cell count and immunohistochemical analysis was detected between the three tested materials. The immunohistochemical analysis revealed the highest immunopositive area fraction in the 3-month evaluation period. The experimental tricalcium silicate cement performed similarly to Biodentine and MTA regarding the osteopontin expression during perforation repair, suggesting it is a suitable alternative with favourable handling characters.","PeriodicalId":14157,"journal":{"name":"International Journal of Experimental Pathology","volume":"103 4","pages":"156-163"},"PeriodicalIF":3.0,"publicationDate":"2022-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9916164","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1