International Journal of Experimental Pathology最新文献_第10页

NLRC5 enhances autophagy via inactivation of AKT/mTOR pathway and ameliorates cardiac hypertrophy NLRC5通过AKT/mTOR通路失活增强自噬，改善心肌肥厚

IF 3 4区医学

International Journal of Experimental Pathology Pub Date : 2021-11-21 DOI: 10.1111/iep.12427

Bayinsilema Ba, Abudoukelimu Mayila, Yankai Guo, Jie Xu, Shifeng Xing, GuiQiu Cao

{"title":"NLRC5 enhances autophagy via inactivation of AKT/mTOR pathway and ameliorates cardiac hypertrophy","authors":"Bayinsilema Ba, Abudoukelimu Mayila, Yankai Guo, Jie Xu, Shifeng Xing, GuiQiu Cao","doi":"10.1111/iep.12427","DOIUrl":"10.1111/iep.12427","url":null,"abstract":"The aim of this study was to investigate the effect of nucleotide-binding oligomerization domain (NOD)-like receptor family CARD domain containing 5 (NLRC5) in cardiac hypertrophy, and to explore the mechanism implicated in this effect Cardiac hypertrophy was induced in neonatal rat cardiac myocytes using 1 μM of angiotensin II (Ang II) for 12, 24 and 48 h. Overexpression of NLRC5 was induced in H9C2 cells, and the NLRC5 + Ang II–treated cells were exposed to SC9 and 3‑methyladenine (3MA). An immunofluorescence assay was used for α-actinin staining, and quantitative real-time reverse transcriptase-polymerase chain reaction (qRT-PCR) was performed for NLRC5, atrial natriuretic peptide (ANP) and brain natriuretic peptide (BNP) determination. Western blot analysis was applied to measure the levels of NLRC5, microtubule-associated protein 1A/1B-light chain 3 type I (LC3I), LC3II, sequestosome 1 (p62), protein kinase B (AKT), phosphorylated Akt (pAKT), mammalian target of rapamycin (mTOR) and phosphorylated mTOR (pmTOR). The level of NLRC5 was significantly decreased after Ang II treatment in cardiomyocytes, but the levels of ANP and BNP were increased. Overexpression of NLRC5 reduced the cell size, downregulated the levels of ANP and BNP, increased LC3II / LC3I, but decreased p62 in Ang II–induced cardiomyocyte hypertrophy. In addition, the results from Western blot showed that overexpression of NLRC5 distinctly decreased the ratios of pAKT/AKT and pmTOR/mTOR in cardiomyocyte hypertrophy. SC79 and 3MA significantly downregulated the ratio of LC3I/LC3II but increased the level of p62 in NLRC5 + Ang II–treated cells. These results provide a possible novel therapeutic strategy for cardiac hypertrophy that might be useful in a clinical setting.","PeriodicalId":14157,"journal":{"name":"International Journal of Experimental Pathology","volume":"103 1","pages":"23-30"},"PeriodicalIF":3.0,"publicationDate":"2021-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8781646/pdf/IEP-103-23.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10594531","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Optogenetic control of cancer cell survival in ChR2-transfected HeLa cells chr2转染的HeLa细胞中癌细胞存活的光遗传学控制

IF 3 4区医学

International Journal of Experimental Pathology Pub Date : 2021-11-17 DOI: 10.1111/iep.12426

Claudio Córdova, Carlo Lozano, Belén Rodríguez, Ivanny Marchant, Rodrigo Zúñiga, Paola Ochova, Pablo Olivero, Wilfredo Alejandro González-Arriagada

{"title":"Optogenetic control of cancer cell survival in ChR2-transfected HeLa cells","authors":"Claudio Córdova, Carlo Lozano, Belén Rodríguez, Ivanny Marchant, Rodrigo Zúñiga, Paola Ochova, Pablo Olivero, Wilfredo Alejandro González-Arriagada","doi":"10.1111/iep.12426","DOIUrl":"10.1111/iep.12426","url":null,"abstract":"Optogenetics is a molecular biological technique involving transfection of cells with photosensitive proteins and the subsequent study of their biological effects. The aim of this study was to evaluate the effect of blue light on the survival of HeLa cells, transfected with channelrhodopsin-2 (ChR2). HeLa wild-type cells were transfected with a plasmid that contained the gene for ChR2. Transfection and channel function were evaluated by real-time polymerase chain reaction (RT-PCR), fluorescence imaging using green fluorescent protein (GFP) and flow cytometry for intracellular calcium changes using a Fura Red probe. We developed a platform for optogenetic stimulation for use within the cell culture incubator. Different stimulation procedures using blue light (467 nm) were applied for up to 24 h. Cell survival was determined by flow cytometry using propidium iodide and rhodamine probes. Change in cell survival showed a statistically significant (p < 0.05) inverse association with the frequency and time of application of the light stimulus. This change seemed to be associated with the ChR2 cis-trans-isomerization cycle. Cell death was associated with high concentrations of calcium in the cytoplasm and stimulation intervals less than the period of isomerization. It is possible to transfect HeLa cells with ChR2 and control their survival under blue light stimulation. We suggest that this practice should be considered in the future development of optogenetic systems in biological or biomedical research.","PeriodicalId":14157,"journal":{"name":"International Journal of Experimental Pathology","volume":"102 6","pages":"242-248"},"PeriodicalIF":3.0,"publicationDate":"2021-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39744305","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Immunohistochemical and immunofluorescence expression profile of lymphatic endothelial cell markers in oral cancer 口腔癌淋巴内皮细胞标志物的免疫组织化学和免疫荧光表达谱

IF 3 4区医学

International Journal of Experimental Pathology Pub Date : 2021-11-17 DOI: 10.1111/iep.12411

Kullasit Chutipongpisit, V. Praveen Parachuru, Lara T. Friedlander, Haizal M. Hussaini, Alison M. Rich

{"title":"Immunohistochemical and immunofluorescence expression profile of lymphatic endothelial cell markers in oral cancer","authors":"Kullasit Chutipongpisit, V. Praveen Parachuru, Lara T. Friedlander, Haizal M. Hussaini, Alison M. Rich","doi":"10.1111/iep.12411","DOIUrl":"10.1111/iep.12411","url":null,"abstract":"Lymphangiogenesis makes an important contribution to the tumour microenvironment (TME), but little is known about this in oral squamous cell carcinoma (OSCC). Archival formalin-fixed paraffin-embedded specimens (28 OSCC, 10 inflamed and 6 normal oral mucosa controls) were processed using immunohistochemistry (IHC) with antibodies against lymphatic markers D2-40 (podoplanin), LYVE-1, VEGFR3 and Prox1. After the endothelial cells had been highlighted by the various markers for lymphatic endothelium, the positive stained cells and vessels were identified and counted in a systematic manner to determine microvessel density. Double-labelling immunofluorescence (DLIF) was used to investigate the specificity of D2-40 and LYVE-1 to lymphatic endothelial cells (LECs) as opposed to blood ECs. There was higher D2-40 and Prox1 lymphatic vessel density (P = .001) in the OSCC group when compared with both control groups. Some malignant keratinocytes expressed lymphatic markers, as did a much smaller number of epithelial cells in the control groups. DLIF showed that no vessels co-expressed D2-40/CD34 or LYVE/CD34. Some D2/40+ LVs were LYVE−. D2-40 was the most specific LEC marker in OSCC tissues. These results establish that the OSCC TME contains significantly more lymphatic vessels expressing D2-40 and Prox1 than the control groups, which may play a role in facilitating lymphatic invasion and metastases.","PeriodicalId":14157,"journal":{"name":"International Journal of Experimental Pathology","volume":"102 6","pages":"268-278"},"PeriodicalIF":3.0,"publicationDate":"2021-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39901968","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correlation between aryl hydrocarbon receptor and IL-17+ and Foxp3+ T-cell infiltration in bladder cancer 膀胱癌中芳烃受体与IL-17+、Foxp3+ t细胞浸润的关系

IF 3 4区医学

International Journal of Experimental Pathology Pub Date : 2021-11-11 DOI: 10.1111/iep.12392

Soheila Fattahi, Monireh Karimi, Mahdi Ghatreh-Samani, Fatemeh Taheri, Hedayatollah Shirzad, Faramarz Mohammad Alibeigi, Maryam Anjomshoa, Nader Bagheri

{"title":"Correlation between aryl hydrocarbon receptor and IL-17+ and Foxp3+ T-cell infiltration in bladder cancer","authors":"Soheila Fattahi, Monireh Karimi, Mahdi Ghatreh-Samani, Fatemeh Taheri, Hedayatollah Shirzad, Faramarz Mohammad Alibeigi, Maryam Anjomshoa, Nader Bagheri","doi":"10.1111/iep.12392","DOIUrl":"10.1111/iep.12392","url":null,"abstract":"Bladder cancer (BC) is one of the most prevalent cancers around the world and, if not treated well, has high morbidity and mortality. Many studies have indicated that there may be various roles for the aryl hydrocarbon receptor (AHR) in the immune system. The aim of this study was to determine the frequency of Foxp3+ regulatory T (Treg) and T helper 17 cells (Th17) in BC tissue in comparison with controls and determine the relationship between AHR, Foxp3+ Treg and Th17 cells in BC. A total of 40 patients with BC were enrolled in this study. The control group was selected from non-tumoural parts of bladder tissues from the patients who have undergone cystoscopy. The percentage of regulatory T cells (Foxp3+/CD4+) and Th17 (IL-17+/CD4+), as well as AHR+ cells in BC tissues and controls, were determined by immunohistochemistry. The results of this study showed that the number of Foxp3+ Treg and Th17 is significantly higher in bladder tumour tissues in comparison with non-tumoural tissues. Also, the percentage of AHR+ lymphocytes and AHR+ cells was increased significantly in bladder tumour tissues rather than non-tumoural tissues. This study also found a relation between AHR and Foxp3+/CD4+ T lymphocytes ratio cells in BC. The percentage of Foxp3+ Tregs and AHR+ cells were significantly correlated with the grade and stage of BC. An increase in the percentage of Foxp3+ Treg and Th17 cells may play an important role in tumour immunity; and determining the relationship between AHR and differentiation of Th17/Foxp3+Treg in BC can lead to a potential cancer therapeutic possibility.","PeriodicalId":14157,"journal":{"name":"International Journal of Experimental Pathology","volume":"102 6","pages":"249-259"},"PeriodicalIF":3.0,"publicationDate":"2021-11-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39611609","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

British Society for Matrix Biology Spring 2021 Meeting: “Inflammation, Fibrosis, Resolution and the Matrix” 英国基质生物学学会2021年春季会议:“炎症，纤维化，分辨率和基质”

IF 3 4区医学

International Journal of Experimental Pathology Pub Date : 2021-11-09 DOI: 10.1111/iep.12409

引用次数: 0

The intensity of the foreign body response to polyether-polyurethane implant in diabetic mice is strain-dependent 糖尿病小鼠对聚醚-聚氨酯植入物的异体反应强度呈品系依赖性。

IF 3 4区医学

International Journal of Experimental Pathology Pub Date : 2021-11-07 DOI: 10.1111/iep.12397

Simone A. de Almeida, Laura A. A. Orellano, Luciana X. Pereira, Celso T. R. Viana, Silvia P. Andrade, Paula P. Campos, Mônica A. N. D. Ferreira

{"title":"The intensity of the foreign body response to polyether-polyurethane implant in diabetic mice is strain-dependent","authors":"Simone A. de Almeida, Laura A. A. Orellano, Luciana X. Pereira, Celso T. R. Viana, Silvia P. Andrade, Paula P. Campos, Mônica A. N. D. Ferreira","doi":"10.1111/iep.12397","DOIUrl":"10.1111/iep.12397","url":null,"abstract":"A number of genetic factors have been linked to the development of diabetes, a condition that often requires implantable devices such as glucose sensors. In normoglycaemic individuals, this procedure induces a foreign body reaction (FBR) that is detrimental to bioimplant functionality. However, the influence of the genetic background on this reaction in diabetes has not been investigated. We examined the components of FBR (capsule thickness, collagen deposition, mast cell and foreign body giant cell number) in subcutaneous implants of polyether polyurethane (SIPP) in streptozotocin (STZ)-induced diabetes in Swiss, C57BL/6 and Balb/c mice. The fasting blood glucose levels before STZ injections were 133.5 ± 5.1 mg/dL, after the treatment increased 68.4% in Swiss mice, 62.4% in C57BL/6 and 30.9% in Balb/c mice. All FBR features were higher in implants of Swiss and C57BL/6 mice compared with those in implants of Balb/c. Likewise, the apoptotic index was higher in implants of diabetic Swiss and C57BL/6 mice whose glycaemic levels were the highest. Our findings show an association between the severity of hyperglycaemic levels and the intensity of the FBR to SIPP. These important strain-related differences in susceptibility to diabetes and the intensity of the FBR must be considered in management using implantable devices in diabetic individuals.","PeriodicalId":14157,"journal":{"name":"International Journal of Experimental Pathology","volume":"102 4-5","pages":"182-191"},"PeriodicalIF":3.0,"publicationDate":"2021-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39599309","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Soy diet induces intestinal inflammation in adult Zebrafish: Role of OTX and P53 family 大豆饮食诱导成年斑马鱼肠道炎症:OTX和P53家族的作用

IF 3 4区医学

International Journal of Experimental Pathology Pub Date : 2021-11-01 DOI: 10.1111/iep.12420

Giovanni Micheloni, Marta Carnovali, Giorgia Millefanti, Manuel Rizzetto, Vittoria Moretti, Giuseppe Montalbano, Francesco Acquati, Cristina Giaroni, Roberto Valli, Lucy Costantino, Fulvio Ferrara, Giuseppe Banfi, Massimo Mariotti, Giovanni Porta

{"title":"Soy diet induces intestinal inflammation in adult Zebrafish: Role of OTX and P53 family","authors":"Giovanni Micheloni, Marta Carnovali, Giorgia Millefanti, Manuel Rizzetto, Vittoria Moretti, Giuseppe Montalbano, Francesco Acquati, Cristina Giaroni, Roberto Valli, Lucy Costantino, Fulvio Ferrara, Giuseppe Banfi, Massimo Mariotti, Giovanni Porta","doi":"10.1111/iep.12420","DOIUrl":"10.1111/iep.12420","url":null,"abstract":"Inflammatory bowel diseases (IBDs) are a group of inflammatory conditions of the colon and small intestine, including Crohn's disease and ulcerative colitis. Since Danio rerio is a promising animal model to study gut function, we developed a soy-dependent model of intestinal inflammation in adult zebrafish. The soya bean meal diet was given for 4 weeks and induced an inflammatory process, as demonstrated by morphological changes together with an increased percentage of neutrophils infiltrating the intestinal wall, which developed between the second and fourth week of treatment. Pro-inflammatory genes such as interleukin-1beta, interleukin-8 and tumour necrosis factor alpha were upregulated in the second week and anti-inflammatory genes such as transforming growth factor beta and interleukin-10. Interestingly, an additional expression peak was found for interleukin-8 at the fourth week. Neuronal genes, OTX1 and OTX2, were significantly upregulated in the first two weeks, compatible with the development of the changes in the gut wall. As for the genes of the p53 family such as p53, DNp63 and p73, a statistically significant increase was observed after two weeks of treatment compared with controls. Interestingly, DNp63 and p73 were shown an additional peak after four weeks. Our data demonstrate that soya bean meal diet negatively influences intestinal morphology and immunological function in adult zebrafish showing the features of acute inflammation. Data observed at the fourth week of treatment may suggest initiation of chronic inflammation. Adult zebrafish may represent a promising model to better understand the mechanisms of food-dependent intestinal inflammation.","PeriodicalId":14157,"journal":{"name":"International Journal of Experimental Pathology","volume":"103 1","pages":"13-22"},"PeriodicalIF":3.0,"publicationDate":"2021-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/1d/c9/IEP-103-13.PMC8781668.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39835752","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

miR-381-3p attenuates doxorubicin resistance in human anaplastic thyroid carcinoma via targeting homeobox A9 miR-381-3p通过靶向同源盒A9减弱人间变性甲状腺癌的阿霉素耐药性

IF 3 4区医学

International Journal of Experimental Pathology Pub Date : 2021-10-31 DOI: 10.1111/iep.12401

Yan Zhang, Ke Li, Weili Wang, Jingjing Han

{"title":"miR-381-3p attenuates doxorubicin resistance in human anaplastic thyroid carcinoma via targeting homeobox A9","authors":"Yan Zhang, Ke Li, Weili Wang, Jingjing Han","doi":"10.1111/iep.12401","DOIUrl":"10.1111/iep.12401","url":null,"abstract":"Abnormal microRNA (miR) expression has frequently been reported to be implicated in cancer-related drug resistance. Herein, we planned to investigate whether miR-381-3p contributes to doxorubicin (DOX) resistance in anaplastic thyroid carcinoma (ATC). DOX-resistant ATC tissues and cell lines were prepared to detect miR-381-3p and homeobox A9 (HOXA9) expression. CCK8, transwell and TUNEL assays were performed to evaluate cell proliferation, migration and invasion, and apoptosis in in vitro experiments. HOXA9 expression is intensively expressed in ATC tissues compared with benign thyroid tissues. Compared with parental ATC cell lines, HOXA9 protein expression is significantly up-regulated in DOX-resistant SW1736 and CAL62 cells. The knockdown of HOXA9 leads to growth inhibition and apoptosis of DOX-resistant SW1736 and CAL62 cells. Our results also indicate a significant decrease in miR-381-3p expression levels in DOX-resistant ATC tissues and cell lines. miR-381-3p may function as a tumour suppressor to impede proliferation, migration and invasion and induce apoptosis of DOX-resistant SW1736 and CAL62 cells by inhibiting HOXA9 protein expression. Our results present a novel signalling axis miR-381-3p/HOXA9 that mediates DOX resistance in ATC. miR-381-3p and HOXA9 may be promising molecular targets for preventing ATC progression and drug resistance.","PeriodicalId":14157,"journal":{"name":"International Journal of Experimental Pathology","volume":"102 4-5","pages":"209-217"},"PeriodicalIF":3.0,"publicationDate":"2021-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39682070","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Evidence for age-related contributions of DNA damage and epigenetics in brain tumorigenesis 脑肿瘤发生中DNA损伤和表观遗传学年龄相关贡献的证据

IF 3 4区医学

International Journal of Experimental Pathology Pub Date : 2021-10-30 DOI: 10.1111/iep.12402

Adrian Tira, Lela Buckingham

{"title":"Evidence for age-related contributions of DNA damage and epigenetics in brain tumorigenesis","authors":"Adrian Tira, Lela Buckingham","doi":"10.1111/iep.12402","DOIUrl":"10.1111/iep.12402","url":null,"abstract":"Glioblastoma (GBM) is a highly malignant primary brain tumour displaying rapid cell proliferation and infiltration. GBM primarily occurs at older age; however, younger populations have also been affected. In GBM and other cancers, genetic and epigenetic alterations promote tumorigenesis causing increased cell proliferation and invasiveness. This investigation explored epigenetic events as contributing factors, especially in gliomas that arise in patients aged 40-60 years. Furthermore, DNA damage in tumours with respect to age was assessed. Archival fixed tissues from 88 cases of glioblastoma and adjacent non-malignant tissues were tested. Global methylation and DNA damage were measured using ELISA detection of 5-methyl cytosine and 8-hydroxy guanine, respectively. IDH mutations and CDKN2 promoter hypermethylation were analysed by pyrosequencing. Tumour tissue was hypomethylated compared with non-malignant tissue (P = .001), and there was a trend towards increased methylation with increasing age. There was a significant increase in DNA damage in patients older than forty years compared with those aged forty years or younger (P = .035). CDKN2 promoter methylation levels followed the age trends of global methylation in this patient group. Patients younger than 60 had more frequently mutated IDH (P = .004). Conclusions: The data support the potential of epigenetic factors in promoting tumorigenesis in younger patients, while increased DNA damage contributes to tumorigenesis in the older patients.","PeriodicalId":14157,"journal":{"name":"International Journal of Experimental Pathology","volume":"102 6","pages":"232-241"},"PeriodicalIF":3.0,"publicationDate":"2021-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39575892","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

miR-942-5p prevents sepsis-induced acute lung injury via targeting TRIM37 miR-942-5p通过靶向TRIM37阻止脓毒症诱导的急性肺损伤

IF 3 4区医学

International Journal of Experimental Pathology Pub Date : 2021-10-30 DOI: 10.1111/iep.12413

Qiang Lu, Dinggao Zhang, Hui liu, Hao Xu

{"title":"miR-942-5p prevents sepsis-induced acute lung injury via targeting TRIM37","authors":"Qiang Lu, Dinggao Zhang, Hui liu, Hao Xu","doi":"10.1111/iep.12413","DOIUrl":"10.1111/iep.12413","url":null,"abstract":"MicroRNAs (miRNAs) have been demonstrated to play pivotal roles in the pathogenesis of sepsis-induced acute lung injury (ALI). In this work, we aimed to clarify the potential role and the underlying mechanism of miR-942-5p in a lipopolysaccharide (LPS)-induced A549 cell injury model. The cell injury was evaluated by CCK-8 assay, flow cytometry and enzyme-linked immunosorbent assay (ELISA). The expression levels of miR-942-5p and tripartite motif-containing protein 37 (TRIM37) were measured by real-time PCR and Western blot, and their association was then validated by bioinformatics, luciferase reporter assay and RNA pull-down assay. We found that the expression of miR-942-5p was decreased in LPS-treated A549 cells. Furthermore, LPS treatment suppressed A549 cell viability, promoted apoptosis and increased the levels of inflammatory cytokines. Conversely, overexpression of miR-942-5p increased cell viability, reduced apoptosis and alleviated inflammatory cytokine secretion in the presence of LPS. Moreover, miR-942-5p directly targeted TRIM37 by binding to the 3′-UTR of TRIM37 mRNA. Upregulation of TRIM37 effectively reversed the anti-apoptotic and anti-inflammatory effects of miR-942-5p in LPS-induced A549 cells. Our findings suggested that miR-942-5p protected against LPS-induced cell injury through inhibiting apoptosis and inflammation in A549 cells by negatively regulating TRIM37.","PeriodicalId":14157,"journal":{"name":"International Journal of Experimental Pathology","volume":"102 4-5","pages":"192-199"},"PeriodicalIF":3.0,"publicationDate":"2021-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39828339","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9