International Journal of Experimental Pathology最新文献

{"title":"Correction to ‘The Energy Density of Laser Light Differentially Modulates the Skin Morphological Reorganization in a Murine Model of Healing by Secondary Intention’","authors":"","doi":"10.1111/iep.70006","DOIUrl":"https://doi.org/10.1111/iep.70006","url":null,"abstract":"<p>Novaes RD, Gonçalves RV, Cupertino MC, Araújo BM, Rezende RM, Santos EC, Leite JPV, Matta SLP. The energy density of laser light differentially modulates the skin morphological reorganization in a murine model of healing by secondary intention. <i>Int J Exp Pathol</i> 2014;95(2):138–146. doi:10.1111/iep.12063</p><p>We apologize for this error.</p>","PeriodicalId":14157,"journal":{"name":"International Journal of Experimental Pathology","volume":"106 6","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/iep.70006","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145317524","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Roles of long non-coding RNAs in oesophageal cancer pathogenesis","authors":"Nan Jiang,&nbsp;Beibei Hu,&nbsp;Xin Shu,&nbsp;Gen Li,&nbsp;Jinguang Wang,&nbsp;Changsheng Lv","doi":"10.1111/iep.70003","DOIUrl":"https://doi.org/10.1111/iep.70003","url":null,"abstract":"<p>With high morbidity and mortality internationally, oesophageal cancer is one of the most common diseases of the digestive system. The most frequent approaches to the treatment of oesophageal cancer are a combination of chemotherapy and radiation therapy. Inevitably, some individuals with oesophageal cancer fail to respond favourably to these treatments because of therapeutic resistance, with eventual unfavourable outcomes. Thus, it would be helpful if patients with oesophageal cancer learned more about the pathophysiology of the disease. Discovering early-detection biomarkers could help to identify prognostic markers. Long non-coding RNAs (lncRNAs) are endogenous RNAs with more than 200 nucleotides that do not code for proteins, and these have been recognised increasingly as being important in cancer. A few of the lncRNAs have been shown to be associated with oesophageal cancer. When target gene expression is inhibited or promoted, lncRNAs can play a role in cell growth, apoptosis and metastasis, among other functions in biology, and are aberrantly produced in oesophageal cancer. Using epithelial–mesenchymal transition (EMT), which is implicated in the occurrence, development and diagnosis of oesophageal cancer, lncRNAs may regulate the natural history of the disease. Thus in this article we have reviewed the biological and molecular mechanisms of lncRNAs, including their role as suppressors of tumours as well as in malignancy, in relationship to oesophageal cancer. Furthermore, we have extrapolated from this analysis some potential A lncRNA candidates for oesophageal cancer detection, prediction and treatment. Lastly, we debate both the potential and the difficulties about how lncRNAs may influence oesophageal cancer.</p>","PeriodicalId":14157,"journal":{"name":"International Journal of Experimental Pathology","volume":"106 5","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/iep.70003","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144923669","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Porcine respiratory disease complex induces pulmonary fibrosis related to the aberrant sphingolipid metabolism","authors":"Xiangfang Tang,&nbsp;Gaokai Li,&nbsp;Lijun Shi,&nbsp;Tao Liu,&nbsp;Zhiyong Si,&nbsp;Guangbo Li,&nbsp;Weiquan Yu,&nbsp;Tao Zhang,&nbsp;Zhenwen Zhao,&nbsp;Xinghui Zhao,&nbsp;Zhanzhong Zhao","doi":"10.1111/iep.70005","DOIUrl":"https://doi.org/10.1111/iep.70005","url":null,"abstract":"<p>Porcine respiratory disease complex (PRDC) is a common syndrome in the modern swine industry worldwide, and its pathogenesis remains unclear to date. Our study aimed to investigate PRDC-induced pulmonary fibrosis and sphingolipid metabolism, and their relationship. Mouse and cell line (A549 and 3D4/21) models exposed to bleomycin and/or transforming growth factor-β1 (TGF-β1) were developed. Histopathological and immunohistochemical staining, colorimetry, lipidomics analysis and pharmacologic intervention assays were used to analyse lung fibrosis and sphingolipid profiles. PRDC was validated by the presence of alveolar epithelial cell (AEC) injury and hyperplasia, inflammatory infiltrates, asymmetric macrophage polarization and mast cell phenotypic changes, TGF-β1 and fibroblast growth factor 2 (FGF-2) overproduction, extensive collagen deposition, foci of fibroblast/myofibroblast with stress fibres (α-SMA, γ-SMA and γ2 actin), cell interaction with increasing frequency, proliferation, apoptosis and autophagy dysregulation, and mucin 6 release—all of which are characteristics of pulmonary fibrosis. Based on the sphingolipidomics and pharmacologic interventions data—the dysregulated sphingolipids, including sphingomyelin (SM), ceramide (Cer), sphingosine-1-phosphate (S1P) and cerebroside (Cb), possibly due to serine palmitoyltransferase (SPT; SPTLC1), ceramide synthase (CerS; CerS2, CerS4), sphingomyelin synthase (SMS; SMS1), neutral sphingomyelinase (NSMase), acid sphingomyelinase (ASMase; SMPDL3B) and sphingosine kinase (SphK; SphK1, SphK2), were found to be closely related to pulmonary fibrosis. Furthermore, d18:1 24:1 SM and 18:1 S1P may be conserved biomarkers and tiamulin fumarate (TF) changes have anti-fibrotic activity. Overall, PRDC induces pulmonary fibrosis, related to the aberrant sphingolipid metabolism, where conserved sphingolipid biomarkers and anti-fibrotic candidates have been found.</p>","PeriodicalId":14157,"journal":{"name":"International Journal of Experimental Pathology","volume":"106 5","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144918669","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular epidemiology of Epstein–Barr virus in gastric cancer patients compared to healthy control group in Shiraz, Iran","authors":"Fatemeh Hosseini Tabatabaie,&nbsp;Sahel Faraji,&nbsp;Mohammad Ansari,&nbsp;Farahnaz Farahani,&nbsp;Zahra Vafapour,&nbsp;Haniyeh Abuei,&nbsp;Seyed Mohammad Ali Hashemi,&nbsp;Mansoureh Shokripour,&nbsp;Navid Omidifar,&nbsp;Ali Farhadi,&nbsp;Jamal Sarvari","doi":"10.1111/iep.70004","DOIUrl":"https://doi.org/10.1111/iep.70004","url":null,"abstract":"<p>Gastric cancer (GC) is the third leading cause of cancer-related mortality worldwide. The Epstein–Barr virus (EBV) may play a role in certain cases of GC. Therefore, this study aimed to investigate the prevalence of EBV in the gastric tissue of both gastric cancer patients and non-cancer patients in Shiraz, Iran. In this cross-sectional study, 159 formalin-fixed paraffin-embedded (FFPE) tissues from gastric cancer patients and 137 from non-cancer patients were assessed. All samples were assessed using PCR on the β-globin gene. The nested PCR method was used to investigate the presence of the EBNA-1-EBV gene. The results were analysed using chi-squared statistical tests. The mean age of the GC group and the control group was 62.08 ± 13.45 and 63.97 ± 9.13. In the GC and control groups, 33.33% (53/159) and 35.04% (48/137) were female. The results showed that in the cancer group, 1.88% (3/159) of tissue samples were positive for EBV, while this statistic was 22.62% (31/137) for non-cancer samples (<i>p</i> &lt; .0001). All EBV-positive cancer patients were female with a mean age of 64.66 ± 7.37 while in the control group, 20.83% (10/48) of females and 23.59% (21/89) of males were positive for EBV, which was not statistically significant (<i>p</i> = .832). The low frequency of EBV infection in GC tissue might indicate the ‘hit and run’ mechanism of EBV in GC carcinogenesis. Additionally, the abundance of B lymphocytes in the inflamed samples of the healthy control group might influence the high frequency of EBV in this group. More investigations are recommended to verify these results.</p>","PeriodicalId":14157,"journal":{"name":"International Journal of Experimental Pathology","volume":"106 5","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144767541","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The role of ketone bodies in oxidized LDL-induced cell proliferation and lipid accumulation of macrophages","authors":"Akira Sato,&nbsp;Hina Nemoto,&nbsp;Ayano Yabuki,&nbsp;Genta Sato,&nbsp;Yuta Ogawa,&nbsp;Makoto Ohira","doi":"10.1111/iep.70002","DOIUrl":"https://doi.org/10.1111/iep.70002","url":null,"abstract":"<p>Ketone bodies (KBs), which include β-hydroxybutyric acid (β-HB) and acetoacetic acid (AcAc), play critical roles in organismal energy homeostasis; however, their effects on atherosclerosis remain unknown. In this study, we investigated the role of β-HB and AcAc on proliferation and lipid accumulation in macrophages by the uptake of oxidized LDL (Ox-LDL), causing the formation of atherosclerotic plaques, using mouse macrophage J774A.1 cells. Both β-HB and AcAc reduced cell proliferation, and AcAc increased lipid accumulation in Ox-LDL-treated J774A.1 cells. Western blotting showed that Ox-LDL decreased the protein expression of two KB-specific receptors, GPR41 and GPR43, both of which are known as potent modulators of inflammation, but had negligible effects on that of the β-HB-specific GPR109A in the cells. These results suggest that Ox-LDL may induce inflammatory responses by decreasing the protein expression of GPR41 and GPR43 in macrophages, and that AcAc, but not β-HB, may exacerbate Ox-LDL-caused atherosclerosis.</p>","PeriodicalId":14157,"journal":{"name":"International Journal of Experimental Pathology","volume":"106 4","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144179071","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exercise combined with corticoid/omega-3 therapy positively affected skeletal and cardiac muscles in middle aged mdx mice","authors":"Paula Andrea Saenz Suarez,&nbsp;Marcos Maciel Junior,&nbsp;Samara Camaçari de Carvalho,&nbsp;Humberto Santo Neto,&nbsp;Elaine Minatel,&nbsp;Maria Julia Marques","doi":"10.1111/iep.70000","DOIUrl":"https://doi.org/10.1111/iep.70000","url":null,"abstract":"<p>Exercise has an important impact on skeletal muscle quality, emerging as an adjuvant therapy to ameliorate muscle inflammation and fibrosis in Duchenne muscular dystrophy (DMD). The aim of the present study was to investigate the benefit of exercise alone or in association with corticoid and omega-3 therapy in the middle aged mdx mouse model of DMD, Mdx mice (12 months of age) performed treadmill exercise (12.4 m/min, for 15 min, twice a week) for 4 weeks. Exercised mdx received deflazacort (1.2 mg/kg; gavage) alone or combined with omega-3 (300 mg/kg; gavage). Sedentary mdx, C57BL/10 and exercised mdx received mineral oil and served as control. At the endpoint (14 months of age), muscle function, respiratory function, electrocardiography (ECG), blood markers of myonecrosis (creatine kinase, CK; alanine aminotransferase, ALT; and aspartate aminotransferase, AST), muscle biomarkers of inflammation and fibrosis (western blot), area of fibrosis and histopathology of tibialis anterior, biceps brachii and diaphragm muscles were evaluated. Exercise and exercise-associated therapies improved behavioural activity (open field), muscle function (grip strength, four limb hanging test, hanging wire test and rotarod), VO₂ consumption, VCO₂ production and ECG. Functional benefits correlated with reduced myonecrosis (decreased CK, ALT, AST) and fibrosis. The muscles studied showed a reduction in inflammation biomarkers (NF-κB, IL-6 and TNF-α) and fibrosis (TGF-β and fibronectin) and an increase in calsequestrin (calcium buffering protein) and PGC1-α (muscle integrity marker). In conclusion, exercise alone or associated with corticosteroid/omega-3 therapy benefited limb, diaphragm and cardiac dystrophic muscles in middle-aged mdx mice.</p>","PeriodicalId":14157,"journal":{"name":"International Journal of Experimental Pathology","volume":"106 3","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143892968","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Role of the tendon circadian clock in tendinopathy and implications for therapeutics","authors":"Ask Møbjerg,&nbsp;Sara Dietz Pedersen,&nbsp;Michael Kjaer,&nbsp;Ching-Yan Chloé Yeung","doi":"10.1111/iep.70001","DOIUrl":"https://doi.org/10.1111/iep.70001","url":null,"abstract":"<p>Tendinopathy or tendon overuse injury is a major clinical problem for individuals and has a significant socio-economic cost. Its pathophysiology is not yet fully understood and involves multiple factors, including mechanical, cellular and molecular factors. The circadian rhythm has been shown to be a major regulator of extracellular matrix (ECM) homeostasis in several connective tissues, including tendon. Very recently, the human patellar tendon has been established as a peripheral clock tissue that exhibits dampening with chronic tendinopathy, and this has important translational and clinical relevance. This review summarises what is currently known about the role of the tendon circadian clock in collagen and tendon ECM homeostasis and proposes a role for circadian clock disruption in tendinopathy. A better understanding of the mechanisms that regulate tendon clock synchronisation could guide the development of new therapeutic strategies for managing tendon overuse injuries.</p>","PeriodicalId":14157,"journal":{"name":"International Journal of Experimental Pathology","volume":"106 3","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/iep.70001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143892969","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Extracellular matrix: Dystroglycan interactions—Roles for the dystrophin-associated glycoprotein complex in skeletal tissue dynamics","authors":"Mark Hopkinson,&nbsp;Andrew A. Pitsillides","doi":"10.1111/iep.12525","DOIUrl":"https://doi.org/10.1111/iep.12525","url":null,"abstract":"<p>Contributions made by the dystrophin-associated glycoprotein complex (DGC) to cell–cell and cell-extracellular matrix (ECM) interactions are vital in development, homeostasis and pathobiology. This review explores how DGC functions may extend to skeletal pathophysiology by appraising the known roles of its major ECM ligands, and likely associated DGC signalling pathways, in regulating cartilage and bone cell behaviour and emergent skeletal phenotypes. These considerations will be contextualised by highlighting the potential of studies into the role of the DGC in isolated chondrocytes, osteoblasts and osteoclasts, and by fuller deliberation of skeletal phenotypes that may emerge in very young mice lacking vital, yet diverse core elements of the DGC. Our review points to roles for individual DGC components—including the glycosylation of dystroglycan itself—beyond the establishment of membrane stability which clearly accounts for severe muscle phenotypes in muscular dystrophy. It implies that the short stature, low bone mineral density, poor bone health and greater fracture risk in these patients, which has been attributed due to primary deficiencies in muscle-evoked skeletal loading, may instead arise due to primary roles for the DGC in controlling skeletal tissue (re)modelling.</p>","PeriodicalId":14157,"journal":{"name":"International Journal of Experimental Pathology","volume":"106 2","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/iep.12525","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143370092","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"KLF9 aggravates the cardiomyocyte hypertrophy in hypertrophic obstructive cardiomyopathy through the lncRNA UCA1/p27 axis","authors":"Dayou Ding,&nbsp;Guangrong Zhao","doi":"10.1111/iep.12526","DOIUrl":"https://doi.org/10.1111/iep.12526","url":null,"abstract":"<p>Cardiac hypertrophy refers to an abnormal increase in the thickness of the heart muscle. Our study explores the role of Krüppel-like factor 9 (KLF9) in hypertrophic obstructive cardiomyopathy (HOCM)-induced cardiomyocyte hypertrophy, providing new targets for the treatment of HOCM. Cardiomyocytes were treated with isoproterenol (ISO). The levels of natriuretic peptide B (BNP)/natriuretic peptide A (ANP)/KLF9/long non-coding RNA urothelial carcinoma-associated 1 (lncRNA UCA1)/p27 were measured. Cell surface area and protein/DNA ratio were tested. The binding between KLF9 and the lncRNA UCA1 promoter and between zeste homologue 2 (EZH2) and lncRNA UCA1 was verified. The enrichment of histone H3 lysine 27 tri-methylation (H3K27me3) and EZH2 on the p27 promoter was analysed. ISO treatment increased KLF9 and lncRNA UCA1 expression and decreased p27 expression in cardiomyocytes. KLF9 knockdown inhibited ISO-induced cardiomyocyte hypertrophy, reduced ANP and BNP expression, and alleviated cardiomyocyte damage. KLF9 activated lncRNA UCA1 expression. LncRNA UCA1 recruited EZH2 to the p27 promoter region, increasing the enrichment of H3K27me3, thereby epigenetically suppressing p27 expression. LncRNA UCA1 overexpression or p27 downregulation reduced the protective effect of KLF9 downregulation on cardiomyocyte hypertrophy. In conclusion, KLF9 activates lncRNA UCA1 expression, and lncRNA UCA1 epigenetically suppresses p27 expression, thereby exacerbating cardiomyocyte hypertrophy in HOCM.</p>","PeriodicalId":14157,"journal":{"name":"International Journal of Experimental Pathology","volume":"106 2","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143248643","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Silencing long non-coding RNA linc00689 suppresses the growth and invasion of osteosarcoma cells by targeting miR-129-5p/NUSAP1","authors":"Ling Zhang,&nbsp;Jingtao Song,&nbsp;Xin Xu,&nbsp;Donghong Sun,&nbsp;Huiting Huang,&nbsp;Yang Chen,&nbsp;Tao Zhang","doi":"10.1111/iep.12524","DOIUrl":"10.1111/iep.12524","url":null,"abstract":"<p>Long non-coding RNAs (lncRNAs) have been reported to play a critical role in the progression and metastasis of osteosarcoma. Recently, long intergenic non-protein coding RNA 689 (linc00689) has been shown to be involved in glioma. However, the precise role of linc00689 in osteosarcoma is unknown. In this study, our data demonstrated that silencing linc00689 by siRNA markedly suppressed the proliferation, migration, invasion and epithelial-mesenchymal transition (EMT) of MG63 and SAOS-2 cells. Bioinformatics analysis and dual-luciferase reporter assay revealed that linc00689 could bind to miR-129-5p. Moreover, NUSAP1 was a target of miR-129-5p and positively regulated by linc00689. Further, NUSAP1 overexpression enhanced MG63 cell behaviour and abolished the inhibitory effects of linc00689 knockdown on the proliferation, migration, invasion and EMT of MG63 cells. In conclusion, linc00689 exerts an oncogenic role in the progression of osteosarcoma, which works via the miR-129-5p/NUSAP1 axis.</p>","PeriodicalId":14157,"journal":{"name":"International Journal of Experimental Pathology","volume":"106 2","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143074483","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}