Silencing long non-coding RNA linc00689 suppresses the growth and invasion of osteosarcoma cells by targeting miR-129-5p/NUSAP1

IF 1.8 4区医学 Q3 PATHOLOGY

International Journal of Experimental Pathology Pub Date : 2025-01-31 DOI:10.1111/iep.12524

Ling Zhang, Jingtao Song, Xin Xu, Donghong Sun, Huiting Huang, Yang Chen, Tao Zhang

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引用次数: 0

Abstract

Long non-coding RNAs (lncRNAs) have been reported to play a critical role in the progression and metastasis of osteosarcoma. Recently, long intergenic non-protein coding RNA 689 (linc00689) has been shown to be involved in glioma. However, the precise role of linc00689 in osteosarcoma is unknown. In this study, our data demonstrated that silencing linc00689 by siRNA markedly suppressed the proliferation, migration, invasion and epithelial-mesenchymal transition (EMT) of MG63 and SAOS-2 cells. Bioinformatics analysis and dual-luciferase reporter assay revealed that linc00689 could bind to miR-129-5p. Moreover, NUSAP1 was a target of miR-129-5p and positively regulated by linc00689. Further, NUSAP1 overexpression enhanced MG63 cell behaviour and abolished the inhibitory effects of linc00689 knockdown on the proliferation, migration, invasion and EMT of MG63 cells. In conclusion, linc00689 exerts an oncogenic role in the progression of osteosarcoma, which works via the miR-129-5p/NUSAP1 axis.

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沉默长链非编码RNA linc00689通过靶向miR-129-5p/NUSAP1抑制骨肉瘤细胞的生长和侵袭。

据报道，长链非编码rna （lncRNAs）在骨肉瘤的进展和转移中起着关键作用。最近，长基因间非蛋白编码RNA 689 （linc00689）被证实与胶质瘤有关。然而，linc00689在骨肉瘤中的确切作用尚不清楚。在本研究中，我们的数据表明，用siRNA沉默linc00689可显著抑制MG63和SAOS-2细胞的增殖、迁移、侵袭和上皮-间质转化（EMT）。生物信息学分析和双荧光素酶报告基因实验显示，linc00689可以结合miR-129-5p。此外，NUSAP1是miR-129-5p的靶点，并受到linc00689的正调控。此外，NUSAP1过表达增强了MG63细胞的行为，消除了linc00689敲低对MG63细胞增殖、迁移、侵袭和EMT的抑制作用。总之，linc00689通过miR-129-5p/NUSAP1轴在骨肉瘤的进展中发挥致癌作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

International Journal of Experimental Pathology 医学-病理学

CiteScore

4.50

自引率

3.30%

发文量

审稿时长

>12 weeks

期刊介绍： Experimental Pathology encompasses the use of multidisciplinary scientific techniques to investigate the pathogenesis and progression of pathologic processes. The International Journal of Experimental Pathology - IJEP - publishes papers which afford new and imaginative insights into the basic mechanisms underlying human disease, including in vitro work, animal models, and clinical research. Aiming to report on work that addresses the common theme of mechanism at a cellular and molecular level, IJEP publishes both original experimental investigations and review articles. Recent themes for review series have covered topics as diverse as "Viruses and Cancer", "Granulomatous Diseases", "Stem cells" and "Cardiovascular Pathology".