Neuroprotective Effects of Khaya Anthotheca (Welw.) C.DC (Meliaceae) Decoction on Neurodegeneration Induced by Estrogen Depletion in Rats

IF 1.8 4区医学 Q3 PATHOLOGY

International Journal of Experimental Pathology Pub Date : 2021-12-29 DOI:10.33696/pathology.2.029

Zemo Gamo Franklin, Djiogue Séfirin, S. Faustin, P. Anatole, Babiker Ali, Yousif, Awounfack Charline Florence, Djikem Tadah Rudig Nikanor, Njamen Dieudonne

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引用次数: 1

Abstract

Background: Recently, we reported estrogen-like and anxiolytic effects of the decoction of Khaya anthotheca in ovariectomized rats. Rationale: The purpose of the present study was to assess the putative neuroprotective properties of the plant in ovariectomized rats. Methods: Thirty female Wistar rats were ovariectomized, while 6 were used as sham. After 14 days of endogenous hormonal decline, animals were randomly divided into six groups (n=6) and administered with distilled water, K. anthotheca decoction (125, 250 and 500 mg/kg doses) or estradiol valerate (1 mg/kg) for 28 days. Phytochemical analysis and antioxidant potential of the decoction were determined. Levels of oxidative stress biomarkers were determined in brain homogenates, while histopathological analysis was performed on brain sections, and expressions of neuroinflammation markers determined. Results: Polyphenols were detected in K. anthotheca, and the ferric reducing antioxidant power (FRAP) assay revealed antioxidant properties (635.50 ± 0.58 mg eq quercetin/g of dried decoction). Treatment with K. anthotheca decoction reduced MDA and increased GSH levels in brain homogenates (p<0.01). As estradiol valerate, the decoction, prevented neurodegeneration observed in cortices and hippocampi of untreated ovariectomized animals. Conclusions: Our results suggested that K. anthotheca is endowed with neuroprotective effects and warrant further studies, including other models of neurodegeneration and dementia.

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卡亚花茶的神经保护作用丁香汤对雌激素缺失大鼠神经退行性变的影响

背景:最近，我们报道了Khaya花茶汤对去卵巢大鼠的雌激素样和抗焦虑作用。理由:本研究的目的是评估该植物对去卵巢大鼠的神经保护作用。方法:切除雌性Wistar大鼠30只，假手术6只。内源性激素下降14 d后，随机分为6组(n=6)，分别给予蒸馏水、花茶汤剂(剂量为125、250和500 mg/kg)或戊酸雌二醇(剂量为1 mg/kg)治疗28 d。对其进行植物化学分析和抗氧化活性测定。在脑匀浆中测定氧化应激生物标志物的水平，同时在脑切片上进行组织病理学分析，并测定神经炎症标志物的表达。结果:花茶中检测到多酚类物质，铁还原抗氧化能力(FRAP)测定显示抗氧化性能(槲皮素635.50±0.58 mg /g)。花茶汤剂降低了大鼠脑匀浆MDA水平，提高了GSH水平(p<0.01)。由于戊酸雌二醇，该煎剂可预防未经治疗的卵巢切除动物的皮质和海马神经退行性变。结论:我们的研究结果表明花茶具有神经保护作用，值得进一步研究，包括其他神经变性和痴呆模型。

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来源期刊

International Journal of Experimental Pathology 医学-病理学

CiteScore

4.50

自引率

3.30%

发文量

审稿时长

>12 weeks

期刊介绍： Experimental Pathology encompasses the use of multidisciplinary scientific techniques to investigate the pathogenesis and progression of pathologic processes. The International Journal of Experimental Pathology - IJEP - publishes papers which afford new and imaginative insights into the basic mechanisms underlying human disease, including in vitro work, animal models, and clinical research. Aiming to report on work that addresses the common theme of mechanism at a cellular and molecular level, IJEP publishes both original experimental investigations and review articles. Recent themes for review series have covered topics as diverse as "Viruses and Cancer", "Granulomatous Diseases", "Stem cells" and "Cardiovascular Pathology".