International Journal of Experimental Pathology最新文献

{"title":"Circular RNAs hsa_circ_0001438 and hsa_circ_0000417 are downregulated and upregulated, respectively, in hepatocellular carcinoma","authors":"Sachiko Imanishi,&nbsp;Shoko Nagata,&nbsp;Toshitsugu Fujita,&nbsp;Hodaka Fujii","doi":"10.1111/iep.12457","DOIUrl":"10.1111/iep.12457","url":null,"abstract":"<p>Hepatocellular carcinoma (HCC) is the most predominant type of liver cancer and is frequently fatal. Alpha-fetoprotein, alpha-fetoprotein-L3, and protein induced by vitamin K absence or antagonist-II are used as biomarkers to diagnose HCC. However, these biomarkers are not highly specific, especially for early-stage HCC diagnosis; therefore, more specific biomarkers are needed. Recently, circular RNA (circRNA) biomarkers have been used to diagnose several intractable diseases. In this study, we sought to identify circRNA biomarkers for the specific diagnosis of HCC. To this end, we compared the expression levels of circRNAs in primary HCC and normal tissues using publicly available RNA-seq data. Our analysis revealed that the expression levels of eight circRNAs were altered in primary HCC tissues compared with normal tissues. To confirm our findings, we examined the expression levels of selected circRNAs in HCC cell lines and normal hepatocytes. The expression level of hsa_circ_0001438, a circRNA that was downregulated in primary HCC, was lower in poorly and well-differentiated HCC cell lines than in normal hepatocytes. By contrast, the expression level of hsa_circ_0000417, which was increased in primary HCC, was strongly upregulated in a well-differentiated HCC cell line compared with normal hepatocytes. Thus, hsa_circ_0001438 and hsa_circ_0000417 might be potential biomarkers for the specific diagnosis of HCC. The experimental strategy described here, using publicly available RNA-seq data, is a useful and cost-effective method of identifying circRNA biomarkers.</p>","PeriodicalId":14157,"journal":{"name":"International Journal of Experimental Pathology","volume":"103 6","pages":"245-251"},"PeriodicalIF":3.0,"publicationDate":"2022-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33493509","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Adenosquamous Carcinoma of Ascending Colon: Where an Unseen is Seen","authors":"Prachi, H. Aiyer","doi":"10.33696/pathology.3.031","DOIUrl":"https://doi.org/10.33696/pathology.3.031","url":null,"abstract":"The frequency of adenosquamous carcinoma of the colon is <0.1% [1-3]. The first case of colorectal adenosquamous carcinoma was reported by Herxheimer in 1907 and was described as a tumor with both adenocarcinoma and squamous cell carcinoma components. These tumors have a greater potential to metastasize as compared to conventional adenocarcinoma of the colon. Adenosquamous carcinoma, located in the ascending colon is rare and has a poor prognosis. Thus, early detection of adenosquamous carcinoma is important and treatment of choice is surgical resection followed by adjuvant chemotherapy. An adenosquamous carcinoma in the lower gastrointestinal tract is a rare malignancy with a frequency of 0.020.06% [4].","PeriodicalId":14157,"journal":{"name":"International Journal of Experimental Pathology","volume":"104 1","pages":""},"PeriodicalIF":3.0,"publicationDate":"2022-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80682796","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Proliferative Changes of Renal Afferent Arteriolar Walls and Renin-Angiotensin-Aldosterone System Inhibitors","authors":"","doi":"10.33696/pathology.3.035","DOIUrl":"https://doi.org/10.33696/pathology.3.035","url":null,"abstract":"","PeriodicalId":14157,"journal":{"name":"International Journal of Experimental Pathology","volume":"210 4","pages":""},"PeriodicalIF":3.0,"publicationDate":"2022-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72416654","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Can Artificial Intelligence Help?","authors":"","doi":"10.33696/pathology.3.033","DOIUrl":"https://doi.org/10.33696/pathology.3.033","url":null,"abstract":"","PeriodicalId":14157,"journal":{"name":"International Journal of Experimental Pathology","volume":"5 1","pages":""},"PeriodicalIF":3.0,"publicationDate":"2022-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75671217","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Newborns’ Hearing Health Associated with Infectious Diseases in Primary Healthcare Infectious Diseases and Neonatal Hearing Screening","authors":"","doi":"10.33696/pathology.3.034","DOIUrl":"https://doi.org/10.33696/pathology.3.034","url":null,"abstract":"","PeriodicalId":14157,"journal":{"name":"International Journal of Experimental Pathology","volume":"22 1","pages":""},"PeriodicalIF":3.0,"publicationDate":"2022-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72684147","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cancer Stem Cells, Together with Mesenchymal Stem Cells and Other Cooperative Cells, Govern the Initiation and Development of Cancer","authors":"","doi":"10.33696/pathology.3.032","DOIUrl":"https://doi.org/10.33696/pathology.3.032","url":null,"abstract":"","PeriodicalId":14157,"journal":{"name":"International Journal of Experimental Pathology","volume":"194 1","pages":""},"PeriodicalIF":3.0,"publicationDate":"2022-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74478874","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Morphophysiological alterations in fruit-eating bats after oral exposure to deltamethrin","authors":"Jerusa Maria Oliveira,&nbsp;Suellen Silva Condessa,&nbsp;Ana Luiza Fonseca Destro,&nbsp;Graziela Domingues Almeida Lima,&nbsp;Marli do Carmo Cupertino,&nbsp;Silvia Almeida Cardoso,&nbsp;Mariella Bontempo Freitas,&nbsp;Leandro Licursi de Oliveira","doi":"10.1111/iep.12455","DOIUrl":"10.1111/iep.12455","url":null,"abstract":"<p>Deltamethrin (DTM) is a synthetic pyrethroid widely used in the cultivation and management of several crops due to its insecticidal action. Application to crops of pyrethroids such as DTM can result in the exposure of water and fruit consumed by fruit bats having a high pyrethroid content which may be harmful. Therefore the objective of this study was to evaluate the effects of short-term oral exposure of the fruit-eating bats (<i>Artibeus lituratus</i>) to two concentrations of DTM (0.02 and 0.04 mg/kg of papaya) on histopathology of the intestine, liver and kidney. The intestine of the animals exposed to both concentrations showed inflammatory infiltrate, degeneration, necrosis and goblet cell hyperplasia as the most frequent pathologies. Besides, the acid mucins showed an increase in the frequency of non-viable cells. The liver showed hepatocyte vacuolizatio and nuclear enlargement, as well as inflammatory infiltrate and steatosis. The kidneys of the exposed animals showed and inflammatory infiltrate, benign nephrosclerosis, vacuolization and necrosis. Also, DTM reduced nitric oxide synthesis, decreased glomerular diameter and increased glycogen percentage in the proximal tubules. Our results suggest that acute exposure to DTM at low concentrations has the potential to induce pronounced histopathological changes in vital organs, such as intestine, liver and kidney of fruit-eating bats.</p>","PeriodicalId":14157,"journal":{"name":"International Journal of Experimental Pathology","volume":"103 5","pages":"219-230"},"PeriodicalIF":3.0,"publicationDate":"2022-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40345756","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of the Relationship between Capillaroscopic Symptoms and the Severity of Systemic Lupus Erythematous","authors":"F. Shirani, Leila Barahimi","doi":"10.33696/pathology.3.037","DOIUrl":"https://doi.org/10.33696/pathology.3.037","url":null,"abstract":"Objectives: Use of imaging tools can detect some specific pathological changes associated with systemic lupus erythematous (SLE). This study aimed to investigate the relationship between capillaroscopic symptoms and the severity of SLE.\u0000\u0000Methods: This was a cross-sectional study carried out on 56 patients with SLE undergoing capillaroscopy referred to Rasool-e Akram hospital in Tehran in 2018. Capillaroscopy findings were assessed according to demographic characteristics and duration of disease.\u0000\u0000Results: All patients had at least one positive finding related to capillaroscopy. Regarding capillaroscopic findings, abnormal microvascular structure in 37.5%, decreased vascular density in 78.6%, enlarged cap loop in 32.1%, microhemorrhage in 16.1% and neoangiogenesis in 25.0% were observed. The results revealed higher vascular density loss in women and higher neoangiogenesis in affected men and higher rate of abnormal microvascular structure at older ages and microhemorrhage at younger ages. Direct relationship was also found between duration of disease and microhemorrhage.\u0000\u0000Conclusion: Almost all patients with SLE undergoing capillaroscopy had at least one pathophysiological change in the capillary bed. The most common pathophysiological change was decreased vascular density, abnormal microvascular structure and capillary loop enlargement.","PeriodicalId":14157,"journal":{"name":"International Journal of Experimental Pathology","volume":"113 1","pages":""},"PeriodicalIF":3.0,"publicationDate":"2022-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87914659","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quantitative assessment of changes in skeletal muscle injury by computer-aided analysis based on two-dimensional ultrasonography combined with contrast-enhanced ultrasonography and estimated by a modified semi-quantitative scoring system: An experimental study in a contusion model","authors":"Jiaqi Zhao,&nbsp;Hejing Huang,&nbsp;Qi Xu,&nbsp;Qian Pan,&nbsp;Jia Guo","doi":"10.1111/iep.12447","DOIUrl":"10.1111/iep.12447","url":null,"abstract":"<p>The aim of this study was to investigate the potential application of computer-aided analysis in the quantitative assessment of changes in skeletal muscle injury in the rabbit contusion model. Forty healthy rabbits were randomly divided into control (<i>n</i> = 5) and contusion (<i>n</i> = 35) groups. Rabbits in the contusion group were used to construct a muscle contusion model induced by a hammer hitting the right gastrocnemius, while the muscles of rabbits in the control group were non-injured. Two-dimensional ultrasound (2D US) and contrast-enhanced ultrasonography (CEUS) were performed on the rabbits that had received skeletal muscle contusion injury at 1 h, and 1, 3, 7, 14, 21 and 28 days after injury. Afterwards, a multiscale blob feature (MBF) method was used to extract the textural features from the 2D US, and the muscle injuries were quantitatively evaluated. The eight textural parameters of skeletal muscle analysed by MBF at 1 h, and 1, 3 and 7 days post-injury were found to be significantly higher in the contusion group than in the control group (<i>p</i> &lt; .05). On Day 14, the textural parameters (e.g., greyscale mean [Mean], greyscale standard deviation [SDev], number of blobs, average size of blobs, homogeneity of distribution, periodicity of distribution [POD] and irregularity) were also evidently higher in the contusion group than in the control group (<i>p</i> &lt; .05). On Day 28, Mean, SDev and POD in the contusion group were markedly higher (<i>p</i> &lt; .05). After that, the microcirculation in the injured areas increased from Day 7 to Day 21 after injury, but decreased on Day 28 after injury. Thus the quantitative assessment of changes in skeletal muscle injury (SMI) using computer-aided analysis allowed us to describe the geometric features of injured muscle fibres and the microperfusion changes estimated by the modified semi-quantitative scoring system. This provides a scientific basis for the development of a novel approach for the evaluation of SMI and rehabilitation process.</p>","PeriodicalId":14157,"journal":{"name":"International Journal of Experimental Pathology","volume":"103 5","pages":"208-218"},"PeriodicalIF":3.0,"publicationDate":"2022-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40397945","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Histomorphometric and oxidative evaluation of the offspring's testis from type 2 diabetic female rats treated with metformin and pentoxifylline","authors":"Jessica Santana de Oliveira,&nbsp;Alluanan Adelson do Nascimento Silva,&nbsp;Fernanda Carolina Ribeiro Dias,&nbsp;Elizabeth Lopes de Oliveira,&nbsp;Emanuel Felipe de Oliveira Filho,&nbsp;Pierre Castro Soares,&nbsp;Catarina Michelle de Oliveira Ferreira,&nbsp;Valdemiro Amaro da Silva Junior","doi":"10.1111/iep.12446","DOIUrl":"10.1111/iep.12446","url":null,"abstract":"<p>Type 2 diabetes mellitus (T2D) during pregnancy is characterized by high levels of reactive oxygen species and pro-inflammatory factors in the placenta. Once these reactive species reach the foetus, they trigger physiological adaptations that allow the foetus to survive, but programme the organism to develop metabolic disorders in adulthood. The male reproductive system is highly susceptible to foetal programming. This study aimed to investigate the effects of intrauterine exposure to T2D on testicular histomorphometry and redox homeostasis of adult rats and evaluate the effects of maternal treatment with metformin and pentoxifylline. Female rats were induced to T2D, then treated with metformin and pentoxifylline, or co-treated with both drugs. The females were mated, the male offspring were sacrificed on postnatal day 90, and the testicles were collected for analysis. Metformin protected the tubular compartment, with the maintenance of the Sertoli cell population and daily sperm production. Pentoxifylline attenuated the effects of diabetes on Leydig cells, in addition to stimulating testosterone production and lowering lipid peroxidation. Intrauterine exposure to T2D results in important testicular alterations that compromise gonadal function, and the co-treatment with metformin and pentoxifylline may represent a promising therapy that attenuates these effects by combining the positive influences in both the tubular and interstitial compartments of the testicular parenchyma.</p>","PeriodicalId":14157,"journal":{"name":"International Journal of Experimental Pathology","volume":"103 5","pages":"174-189"},"PeriodicalIF":3.0,"publicationDate":"2022-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9482357/pdf/IEP-103-174.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40239099","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}