International Journal of Experimental Pathology最新文献_第5页

Arnica montana L. associated with microcurrent accelerates the dermis reorganisation of skin lesions 与微电流相关的山金车加速皮肤病变的真皮层重组

IF 3 4区医学

International Journal of Experimental Pathology Pub Date : 2023-02-08 DOI: 10.1111/iep.12469

Cresle Andrei Zacarias, Rosimere Farias de Mendonça Florenziano, Thiago Antonio Moretti de Andrade, Andrea Aparecida de Aro, Maria Esméria Corezola do Amaral, Gláucia Maria Tech dos Santos, Marcelo Augusto Marretto Esquisatto

{"title":"Arnica montana L. associated with microcurrent accelerates the dermis reorganisation of skin lesions","authors":"Cresle Andrei Zacarias, Rosimere Farias de Mendonça Florenziano, Thiago Antonio Moretti de Andrade, Andrea Aparecida de Aro, Maria Esméria Corezola do Amaral, Gláucia Maria Tech dos Santos, Marcelo Augusto Marretto Esquisatto","doi":"10.1111/iep.12469","DOIUrl":"10.1111/iep.12469","url":null,"abstract":"The aim of this study was to test the effect of electrical stimulation in association with topical Arnica montana gel on organisational changes in the dermis during tissue repair. An experimental rat incisional skin lesion was used for the study. This involved making an incisional lesion on the dorsum of the animals using a scalpel. Ninety-six animals were used divided into the following groups: control (C), microcurrent (MC); topical treatment with Arnica montana gel (ARN); the ARN + microcurrent (ARN + MC). Treatments were administered daily, and injured tissue samples were collected and processed on Days 2, 6 and 10 for dermis analyses. Myeloperoxidase levels were greater in control than in treatment groups on Days 2 and 6. F4/80 expression was similar among all treatment groups and greater than that in control on Day 2. On Day 6, the expression of vascular endothelial growth factor was higher in the MC group than that in other groups, whereas transforming growth factor-β expression increased in the MC and ARN + MC groups on Day 10. The expression of matrix metalloproteinase-2 was higher in the ARN + MC group when compared with other groups on Day 10. Expression levels of collagen I were increased in the ARN and ARN + MC groups when compared with control and MC groups on Day 6, while expression of collagen III was enhanced in MC, ARN, and ARN + MC groups when compared with the control. The protocol combining microcurrent with topical application of ARN reduces the inflammatory process, increases myofibroblasts proliferation and decreases the presence of macrophages in the dermis during skin repair in rats.","PeriodicalId":14157,"journal":{"name":"International Journal of Experimental Pathology","volume":"104 2","pages":"81-95"},"PeriodicalIF":3.0,"publicationDate":"2023-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/iep.12469","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9118991","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The frequency of dysplastic branching crypts in colorectal polypoid tubular adenomas 结直肠息肉样管状腺瘤中分支隐窝发育不良的发生率

IF 3 4区医学

International Journal of Experimental Pathology Pub Date : 2023-02-03 DOI: 10.1111/iep.12466

Carlos A. Rubio, Michael Vieth, Corinna Lang-Schwarz

{"title":"The frequency of dysplastic branching crypts in colorectal polypoid tubular adenomas","authors":"Carlos A. Rubio, Michael Vieth, Corinna Lang-Schwarz","doi":"10.1111/iep.12466","DOIUrl":"10.1111/iep.12466","url":null,"abstract":"Dysplastic crypt branching (DCB) was recently found in ulcerative colitis-associated dysplasia. The aim was to assess the frequency and the branching phenotype of DCB in polypoid colorectal tubular adenomas (TA). A total of 3956 DCB were found in the 139 TA: 98% were in asymmetric branching (DCAB) and the remaining 2% in symmetric branching (DCSB). A linear correlation was found between DCB frequency and the increasing digital size in TA (p < .05). Using a digital ruler, adenomas were divided into small TA (<5 mm) and larger TA (≥5 mm). The difference between the frequency of DCB in small TA (n = 75) vs. larger TA (n = 64), was significant (p < .05). DCB frequency was not influenced by age, gender or TA localization. In the normal colorectal mucosa (≈2 m2), only occasional CSB is found and no CAB. And yet, multiple DCB (mean 16.7 DCB), mostly DCAB, was found in small TA, occupying <5 mm of the mucosal area. In larger TA, as many as 42.1 DCB (mean), mostly DCAB, occurred in merely 7.8 mm (mean) of the colon mucosa. Thus it is suggested that DCB is a standard histologic element of TA. The natural expansion of the adenomatous tissue in larger TA appears to be follow on from newly produced, mostly DCAB, by DCSB and by the accumulation of their dysplastic offspring's progenies. The findings strongly suggest that DCB is a central microstructure in the histological events unfolding in polypoid colorectal TA.","PeriodicalId":14157,"journal":{"name":"International Journal of Experimental Pathology","volume":"104 3","pages":"100-106"},"PeriodicalIF":3.0,"publicationDate":"2023-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/2c/47/IEP-104-100.PMC10182366.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9472258","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

MIMT1 and LINC01550 are uncharted lncRNAs down-regulated in colorectal cancer MIMT1和LINC01550是在结直肠癌中下调的未知lncrna

IF 3 4区医学

International Journal of Experimental Pathology Pub Date : 2023-02-02 DOI: 10.1111/iep.12467

Faramarz Vejdandoust, Rahmaneh Moosavi, Nasrin Fattahi Dolatabadi, Atefeh Zamani, Hossein Tabatabaeian

{"title":"MIMT1 and LINC01550 are uncharted lncRNAs down-regulated in colorectal cancer","authors":"Faramarz Vejdandoust, Rahmaneh Moosavi, Nasrin Fattahi Dolatabadi, Atefeh Zamani, Hossein Tabatabaeian","doi":"10.1111/iep.12467","DOIUrl":"10.1111/iep.12467","url":null,"abstract":"Incomplete knowledge of the molecular basis of colorectal cancer, with subsequent limitations in early diagnosis and effective treatment, has contributed to this form of malignancy becoming the second most common cause of cancer-related death worldwide. With the advances in high-throughput profiling techniques and the availability of public data sets such as The Cancer Genome Atlas Program (TCGA), a broad range of coding transcripts have been profiled and their underlying modes of action have been mapped. However, there is still a huge gap in our understanding of noncoding RNA dysregulation. To this end, we used a bioinformatics approach to shortlist and evaluate yet-to be-profiled long noncoding RNAs (lncRNAs) in colorectal cancer. We analysed the TCGA RNA-seq data and followed this by validating the expression patterns using a qPCR technique. Analysing in-house clinical samples, the real-time PCR method revealed that the shortlisted lncRNAs, that is MER1 Repeat Containing Imprinted Transcript 1 (MIMT1) and Non-Protein Coding RNA 1550 (LINC01550), were down-regulated in colorectal cancer tumours compared with the paired adjacent normal tissues. Mechanistically, the in silico results suggest that LINC01550 could form a complex competitive endogenous RNA (ceRNA) network leading to the subsequent regulation of colorectal cancer-related genes, such as CUGBP Elav-Like Family Member (CELF2), Polypyrimidine Tract Binding Protein 1 (PTBP1) and ELAV Like RNA Binding Protein 1 (ELAV1). The findings of this work indicate that MIMT1 and LINC01550 could be novel tumour suppressor genes that can be studied further to assess their roles in regulating the cancer signalling pathway(s).","PeriodicalId":14157,"journal":{"name":"International Journal of Experimental Pathology","volume":"104 3","pages":"107-116"},"PeriodicalIF":3.0,"publicationDate":"2023-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/iep.12467","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9484036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Apoptosis versus necrosis in tubal ectopic pregnancies following Methotrexate 甲氨蝶呤治疗后输卵管异位妊娠的细胞凋亡与坏死

IF 3 4区医学

International Journal of Experimental Pathology Pub Date : 2023-01-24 DOI: 10.1111/iep.12465

Yaron Gil, Asia Zubkov, Jacques Balayla, Aviad Cohen, Ishai Levin

{"title":"Apoptosis versus necrosis in tubal ectopic pregnancies following Methotrexate","authors":"Yaron Gil, Asia Zubkov, Jacques Balayla, Aviad Cohen, Ishai Levin","doi":"10.1111/iep.12465","DOIUrl":"10.1111/iep.12465","url":null,"abstract":"Methotrexate administration for the treatment of tubal ectopic pregnancies has been shown to cause tubal mass enlargement. Our hypothesis was that, by administrating Methotrexate, a local necrotic reaction occurs, leading to hematoma formation and eventually fallopian tube rupture. Salpingectomy specimens were collected, analysed and divided into three equal groups: patients who received Methotrexate but who ultimately failed medical treatment, patients who had a viable ectopic pregnancy and patients with a self-resolving ectopic pregnancy that were operated due to other medical indications. The specimens were dyed using the Cleaved Caspase-3 (Asp175) Rabbit mA. Specimens were divided into three equal groups and analysed. The patients in self-resolving ectopic pregnancy group were older and had more pregnancies. Rates of apoptosis were found to be less than 1% per slide. Necrosis was not evident in any of the pathological specimens. It seems Methotrexate administration does not lead to a significant tubal necrotic reaction. Further studies are required.","PeriodicalId":14157,"journal":{"name":"International Journal of Experimental Pathology","volume":"104 2","pages":"76-80"},"PeriodicalIF":3.0,"publicationDate":"2023-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/iep.12465","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9472086","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synergistic activation of genes promoting invasiveness by dual deprivation in oxygen and nutrients 通过氧气和营养双重剥夺促进侵袭性的基因协同激活

IF 3 4区医学

International Journal of Experimental Pathology Pub Date : 2023-01-24 DOI: 10.1111/iep.12464

Charly Jehanno, Yann Le Page, Gilles Flouriot, Pascale Le Goff, Denis Michel

{"title":"Synergistic activation of genes promoting invasiveness by dual deprivation in oxygen and nutrients","authors":"Charly Jehanno, Yann Le Page, Gilles Flouriot, Pascale Le Goff, Denis Michel","doi":"10.1111/iep.12464","DOIUrl":"10.1111/iep.12464","url":null,"abstract":"By depriving cancer cells of blood supplies of oxygen and nutrients, anti-angiogenic therapy is aimed at simultaneously asphyxiating and starving the cells. But in spite of its apparent logic, this strategy is generally counterproductive over the long term as the treatment seems to elicit malignancy. Since a defect of blood supply is expected to deprive tumours simultaneously of oxygen and nutrients naturally, we examine here these two deprivations, alone or in combination, on the phenotype and signalling pathways of moderately aggressive MCF7 cancer cells. Each deprivation induces some aspects of the aggressive and migratory phenotypes through activating several pathways, including HIF1-alpha as expected, but also SRF/MRTFA and TCF4/beta-catenin. Strikingly, the dual deprivation has strong cooperative effects on the upregulation of genes increasing the metastatic potential, such as four and a half LIM domains 2 (FHL2) and HIF1A-AS2 lncRNA, which have response elements for both pathways. Using anti-angiogenic agents as monotherapy is therefore questionable as it may give falsely promising short-term tumour regression, but could ultimately exacerbate aggressive phenotypes.","PeriodicalId":14157,"journal":{"name":"International Journal of Experimental Pathology","volume":"104 2","pages":"64-75"},"PeriodicalIF":3.0,"publicationDate":"2023-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/iep.12464","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9125897","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

KLF13 overexpression protects sepsis-induced myocardial injury and LPS-induced inflammation and apoptosis KLF13过表达可保护脓毒症诱导的心肌损伤和lps诱导的炎症和细胞凋亡

IF 3 4区医学

International Journal of Experimental Pathology Pub Date : 2022-12-30 DOI: 10.1111/iep.12459

Ni Zeng, Zaijin Jian, Wenxin Zhu, Junmei Xu, Yongmei Fan, Feng Xiao

{"title":"KLF13 overexpression protects sepsis-induced myocardial injury and LPS-induced inflammation and apoptosis","authors":"Ni Zeng, Zaijin Jian, Wenxin Zhu, Junmei Xu, Yongmei Fan, Feng Xiao","doi":"10.1111/iep.12459","DOIUrl":"10.1111/iep.12459","url":null,"abstract":"Sepsis remains a worldwide public health problem. This study aims to explore the role and mechanism of transcriptional factors (TFs) in sepsis-induced myocardial injury. Firstly, TF KLF13 was selected to explore its role in sepsis-induced myocardial injury. The caecal ligation and puncture (CLP) -induced sepsis mouse model was established and the septic mice were examined using standard histopathological methods. KLF13 expression was detected in the septic mouse heart and was also seen in a lipoploysaccharide (LPS) -induced cellular inflammation model. To explore this further both pro-apoptotic cleaved-caspase3/caspase3 and Bax levels and anti-apoptotic Bcl2 levels were examined, also in both models, In addition inflammatory cytokine (IL-1β, TNF-α, IL-8 and MCP-1) production and IκB-α protein level and p65 phosphorylation were examined in both septic mice and LPS-induced cells. Thus three parameters - cardiomyocyte apoptosis, inflammatory response and NF-κB pathway activation were evaluated under similar conditions. The septic mice showed significant oedema, disordered myofilament arrangement and degradation and necrosis to varying degrees in the myocardial cells. KLF13 was downregulated in both the septic mouse heart and the LPS-induced cellular inflammation model. Furthermore, both models showed abnormally increased cardiomyocyte apoptosis (increased cleaved-caspase3/caspase and Bax protein levels and decreased Bcl2 level), elevated inflammation (increased production of inflammatory cytokines) and the activated NF-κB pathway (increased p65 phosphorylation and decreased IκB-α protein level). KLF13 overexpression notably ameliorated sepsis-induced myocardial injury in vivo and in vitro. KLF13 overexpression protected against sepsis-induced myocardial injury and LPS-induced cellular inflammation and apoptosis via inhibiting the inflammatory pathways (especially NF-κB signalling) and cardiomyocyte apoptosis.","PeriodicalId":14157,"journal":{"name":"International Journal of Experimental Pathology","volume":"104 1","pages":"23-32"},"PeriodicalIF":3.0,"publicationDate":"2022-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/iep.12459","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10726587","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Expression of autocrine motility factor receptor (AMFR) in human breast and lung invasive micropapillary carcinomas 自分泌运动因子受体(AMFR)在乳腺和肺浸润性微乳头状癌中的表达

IF 3 4区医学

International Journal of Experimental Pathology Pub Date : 2022-12-28 DOI: 10.1111/iep.12462

Jing Xu, Hongfei Ma, Qi Wang, Hui Zhang

{"title":"Expression of autocrine motility factor receptor (AMFR) in human breast and lung invasive micropapillary carcinomas","authors":"Jing Xu, Hongfei Ma, Qi Wang, Hui Zhang","doi":"10.1111/iep.12462","DOIUrl":"10.1111/iep.12462","url":null,"abstract":"The aim of this study was to evaluate the clinicopathological significance of autocrine motility factor receptor (AMFR) expression in a variety of human invasive micropapillary carcinomas (IMPC). AMFR expression was compared in 111 samples of a variety of human IMPCs which had intrinsic non-micropapillary components and with 26 cases of control pulmonary adenocarcinoma (CPA, carcinoma without an IMPC component) by immunohistochemistry (IHC). In the 137 cases analysed, AMFR expression was significantly elevated in the IMPC components compared to the non-IMPC components (p = .005) and normal tissues (p < .001). AMFR expression was also higher in the IMPC samples compared to their intrinsic non-IMPC components (p = .0234). Between the 69 cases of lung IMPC and 26 cases of CPA, AMFR expression was notably higher in the IMPC components than in the CPA components (p = .0455). However, there was no significant difference between the non-IMPC components in the lung and the CPA components (p = .4584). Moreover, in breast cancer, elevated AMFR expression was not significantly correlated with mixed type or pure type IMPC (p = .5969) or with age, gender, T stage, or lymph node metastasis (LNM). Between IMPC and CPA of the lung, there was no statistical significance in age, T stage, and LNM, where AMFR expression was higher in IMPC (p = .0071). Thus this study demonstrated that AMFR was overexpressed in a variety of human IMPC components compared with non-micropapillary components. This suggests that AMFR expression is a potential new prognostic indicator for different types of human IMPC, which might thus be a new therapeutic target.","PeriodicalId":14157,"journal":{"name":"International Journal of Experimental Pathology","volume":"104 1","pages":"43-51"},"PeriodicalIF":3.0,"publicationDate":"2022-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/iep.12462","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9280721","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Silencing of RhoC induces macrophage M1 polarization to inhibit migration and invasion in colon cancer via regulating the PTEN/FOXO1 pathway RhoC沉默诱导巨噬细胞M1极化，通过调控PTEN/FOXO1通路抑制结肠癌的迁移和侵袭

IF 3 4区医学

International Journal of Experimental Pathology Pub Date : 2022-12-28 DOI: 10.1111/iep.12460

Bin Yang, Lihua Wang, Zhiying Tian

{"title":"Silencing of RhoC induces macrophage M1 polarization to inhibit migration and invasion in colon cancer via regulating the PTEN/FOXO1 pathway","authors":"Bin Yang, Lihua Wang, Zhiying Tian","doi":"10.1111/iep.12460","DOIUrl":"10.1111/iep.12460","url":null,"abstract":"Ras homologue family member C (RhoC) is an oncogene in diverse types of human cancers, whereas its regulatory mechanisms involving macrophage polarization is rarely investigated. This study is designed to explore the regulatory role of RhoC in colon cancer and the underlying molecular mechanisms involving macrophage polarization. We detected RhoC expression by quantitative real-time polymerase chain reaction (qRT-PCR) and western blot, and analysed the biological function of RhoC knockdown in CC cells by the MTT, wound healing and transwell assay. Macrophage polarization-associated markers, genes associated with migration, phosphatase and tensin homologue (PTEN) and forkhead box O (FOXO) were determined by qRT-PCR and western blot. The xenograft tumour mouse model was used to assess the role of RhoC in vivo. RhoC is highly expressed in CC cells. The cell viability, invasion and migration abilities of CC cells were reduced by knockdown of RhoC. RhoC knockdown promoted M1 polarization, inhibited M2 polarization and decreased levels of genes associated with migration (matrix metalloproteinase-2 and matrix metalloproteinase-9). Silencing of RhoC inhibited tumour growth and expression of genes associated with migration in the xenografted model. In addition, silencing of RhoC promoted PTEN/FOXO1 expression, and PTEN inhibitor (SF1670) reversed the inhibitory effects of RhoC silencing. We demonstrated that silencing of RhoC reduced CC cells invasion and migration, and tumour growth by suppressing M2 macrophage polarization via regulating the PTEN/FOXO1 pathway.","PeriodicalId":14157,"journal":{"name":"International Journal of Experimental Pathology","volume":"104 1","pages":"33-42"},"PeriodicalIF":3.0,"publicationDate":"2022-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/iep.12460","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9280723","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Cilostazol attenuates oxidative stress and apoptosis in the quadriceps muscle of the dystrophic mouse experimental model 西洛他唑可减轻营养不良小鼠四头肌氧化应激和细胞凋亡

IF 3 4区医学

International Journal of Experimental Pathology Pub Date : 2022-12-24 DOI: 10.1111/iep.12461

Túlio de Almeida Hermes, Rafael Dias Mâncio, Daniela Sayuri Mizobutti, Aline Barbosa Macedo, Larissa Akemi Kido, Valéria Helena Alves Cagnon Quitete, Elaine Minatel

{"title":"Cilostazol attenuates oxidative stress and apoptosis in the quadriceps muscle of the dystrophic mouse experimental model","authors":"Túlio de Almeida Hermes, Rafael Dias Mâncio, Daniela Sayuri Mizobutti, Aline Barbosa Macedo, Larissa Akemi Kido, Valéria Helena Alves Cagnon Quitete, Elaine Minatel","doi":"10.1111/iep.12461","DOIUrl":"10.1111/iep.12461","url":null,"abstract":"Duchenne muscular dystrophy (DMD) is the most severe and frequent form of muscular dystrophy. The mdx mouse is one of the most widely used experimental models to understand aspects of the biology of dystrophic skeletal muscles and the mechanisms of DMD. Oxidative stress and apoptosis are present in early stages of the disease in mdx mice. The high production of reactive oxygen species (ROS) causes activation of apoptotic death regulatory proteins due to DNA damage and breakdown of nuclear and mitochondrial membranes. The quadriceps (QUA) muscle of the mdx mouse is a good tool to study oxidative events. Previous studies have demonstrated that cilostazol exerts an anti-oxidant effect by decreasing the production of reactive oxygen species (ROS). The present study aimed to evaluate the ability of cilostazol to modulate oxidative stress and apoptosis in the QUA muscle of mdx mice. Fourteen-day-old mdx mice received cilostazol or saline for 14 days. C57BL/10 mice were used as a control. In the QUA muscle of mdx mice, cilostazol treatment decreased ROS production (−74%), the number of lipofuscin granules (−47%), lipid peroxidation (−11%), and the number of apoptotic cells (−66%). Thus cilostazol showed anti-oxidant and anti-apoptotic action in the QUA muscle of mdx mice.","PeriodicalId":14157,"journal":{"name":"International Journal of Experimental Pathology","volume":"104 1","pages":"13-22"},"PeriodicalIF":3.0,"publicationDate":"2022-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/iep.12461","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10736136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Protection of dystrophic muscle cells using Idebenone correlates with the interplay between calcium, oxidative stress and inflammation 利用依地苯酮保护营养不良的肌肉细胞与钙、氧化应激和炎症之间的相互作用有关

IF 3 4区医学

International Journal of Experimental Pathology Pub Date : 2022-12-24 DOI: 10.1111/iep.12463

Amanda Harduim Valduga, Daniela Sayuri Mizobuti, Fernanda dos Santos Rapucci Moraes, Rafael Dias Mâncio, Luis Henrique Rapucci Moraes, Túlio de Almeida Hermes, Aline Barbosa Macedo, Elaine Minatel

{"title":"Protection of dystrophic muscle cells using Idebenone correlates with the interplay between calcium, oxidative stress and inflammation","authors":"Amanda Harduim Valduga, Daniela Sayuri Mizobuti, Fernanda dos Santos Rapucci Moraes, Rafael Dias Mâncio, Luis Henrique Rapucci Moraes, Túlio de Almeida Hermes, Aline Barbosa Macedo, Elaine Minatel","doi":"10.1111/iep.12463","DOIUrl":"10.1111/iep.12463","url":null,"abstract":"There is strong cross-talk between abnormal intracellular calcium concentration, high levels of reactive oxygen species (ROS) and an exacerbated inflammatory process in the dystrophic muscles of mdx mice, the experimental model of Duchenne muscular dystrophy (DMD). In this study, we investigated effects of Idebenone, a potent anti-oxidant, on oxidative stress markers, the anti-oxidant defence system, intracellular calcium concentrations and the inflammatory process in primary dystrophic muscle cells from mdx mice. Dystrophic muscle cells were treated with Idebenone (0.05 μM) for 24 h. The untreated mdx muscle cells were used as controls. The MTT assay showed that Idebenone did not have a cytotoxic effect on the dystrophic muscle cells. The Idebenone treatment was able to reduce the levels of oxidative stress markers, such as H2O2 and 4-HNE, as well as decreasing intracellular calcium influx in the dystrophic muscle cells. Regarding Idebenone effects on the anti-oxidant defence system, an up-regulation of catalase levels, glutathione reductase (GR), glutathione peroxidase (GPx) and superoxide dismutase (SOD) activity was observed in the dystrophic muscle cells. In addition, the Idebenone treatment was also associated with reduction in inflammatory molecules, such as nuclear factor kappa-B (NF-κB) and tumour necrosis factor (TNF) in mdx muscle cells. These outcomes supported the use of Idebenone as a protective agent against oxidative stress and related signalling mechanisms involved in dystrophinopathies, such as DMD.","PeriodicalId":14157,"journal":{"name":"International Journal of Experimental Pathology","volume":"104 1","pages":"4-12"},"PeriodicalIF":3.0,"publicationDate":"2022-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/iep.12463","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10736137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3