International Journal of Experimental Pathology最新文献_第4页

A prognostic model based on regulatory T-cell-related genes in gastric cancer: Systematic construction and validation 基于调节性t细胞相关基因的胃癌预后模型:系统构建与验证

IF 3 4区医学

International Journal of Experimental Pathology Pub Date : 2023-06-23 DOI: 10.1111/iep.12487

Qin Tong, Yingjie Ling

{"title":"A prognostic model based on regulatory T-cell-related genes in gastric cancer: Systematic construction and validation","authors":"Qin Tong, Yingjie Ling","doi":"10.1111/iep.12487","DOIUrl":"10.1111/iep.12487","url":null,"abstract":"Human gastrointestinal tumours have been shown to contain massive numbers of tumour infiltrating regulatory T cells (Tregs), the presence of which are closely related to tumour immunity. This study was designed to develop new Treg-related prognostic biomarkers to monitor the prognosis of patients with gastric cancer (GC). Treg-related prognostic genes were screened from Treg-related differentially expressed genes in GC patients by using Cox regression analysis, based on which a prognostic model was constructed. Then, combined with RiskScore, survival curve, survival status assessment and ROC analysis, these genes were used to verify the accuracy of the model, whose independent prognostic ability was also evaluated. Six Treg-related prognostic genes (CHRDL1, APOC3, NPTX1, TREML4, MCEMP1, GH2) in GC were identified, and a 6-gene Treg-related prognostic model was constructed. Survival analysis revealed that patients had a higher survival rate in the low-risk group. Combining clinicopathological features, we performed univariate and multivariate regression analyses, with results establishing that the RiskScore was an independent prognostic factor. Predicted 1-, 3- and 5-year survival rates of GC patients had a good fit with the actual survival rates according to nomogram results. In addition patients in the low-risk group had higher tumour mutational burden (TMB) values. Gene Set Enrichment Analysis (GSEA) demonstrated that genes in the high-risk group were significantly enriched in pathways related to immune inflammation, tumour proliferation and migration. In general, we constructed a 6-gene Treg-associated GC prognostic model with good prediction accuracy, where RiskScore could act as an independent prognostic factor. This model is expected to provide a reference for clinicians to estimate the prognosis of GC patients.","PeriodicalId":14157,"journal":{"name":"International Journal of Experimental Pathology","volume":"104 5","pages":"226-236"},"PeriodicalIF":3.0,"publicationDate":"2023-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/iep.12487","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10256903","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

RUNX1 promotes liver fibrosis progression through regulating TGF-β signalling RUNX1通过调节TGF-β信号传导促进肝纤维化进展

IF 3 4区医学

International Journal of Experimental Pathology Pub Date : 2023-04-17 DOI: 10.1111/iep.12474

Zhaoyang Guo, Xinxin Liu, Shulei Zhao, Fengkai Sun, Wanhua Ren, Mingze Ma

{"title":"RUNX1 promotes liver fibrosis progression through regulating TGF-β signalling","authors":"Zhaoyang Guo, Xinxin Liu, Shulei Zhao, Fengkai Sun, Wanhua Ren, Mingze Ma","doi":"10.1111/iep.12474","DOIUrl":"10.1111/iep.12474","url":null,"abstract":"Liver fibrosis is caused by chronic liver injury. There are limited treatments for it, and the pathogenesis is unclear. Therefore, there is an urgent need to explore the pathogenesis of liver fibrosis, and to try to identify new potential therapeutic targets. For this study we used the carbon tetrachloride abdominal injection induced liver fibrosis animal model in mice. Primary hepatic stellate cell isolation was performed by a density-gradient separation method, and this was followed by immunofluorescence stain analyses. Signal pathway analysis was performed by dual-luciferase reporter assay and western blotting. Our results showed that RUNX1 was upregulated in cirrhotic liver tissues compared with normal liver tissues. Besides, overexpression of RUNX1 caused more severe liver fibrosis lesions than control group under CCl4-induced conditions. Moreover, α-SMA expression in the RUNX1 overexpression group was significantly higher than in the control group. Interestingly, we found that RUNX1 could promote the activation of TGF-β/Smads in a dual-luciferase reporter assay. Thus we demonstrated that RUNX1 could be considered as a new regulator of hepatic fibrosis by activating TGF-β/Smads signalling. Based on this, we concluded that RUNX1 may be developed as a new therapeutic target in the treatment of liver fibrosis in the future. In addition, this study also provides a new insight about the aetiology of liver fibrosis.","PeriodicalId":14157,"journal":{"name":"International Journal of Experimental Pathology","volume":"104 4","pages":"188-198"},"PeriodicalIF":3.0,"publicationDate":"2023-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/iep.12474","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10271542","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Abnormal expression and role of MicroRNA-214-3p/SLC8A1 in neonatal Hypoxic-Ischaemic encephalopathy MicroRNA-214-3p/SLC8A1在新生儿缺氧缺血性脑病中的异常表达及其作用

IF 3 4区医学

International Journal of Experimental Pathology Pub Date : 2023-04-09 DOI: 10.1111/iep.12475

Liu Yang, Li Zhang, Jing Zhu, Yuqian Wang, Ning Zou, Zhengjuan Liu, Yingjie Wang

引用次数: 1

High density of FOXP3 predicts better prognosis in germinal and non-germinal centre diffuse large B-cell lymphoma FOXP3的高密度预示着生发和非生发中心弥漫性大b细胞淋巴瘤更好的预后

IF 3 4区医学

International Journal of Experimental Pathology Pub Date : 2023-03-28 DOI: 10.1111/iep.12477

Asmaa M. Ahmed, Sally S. Abdel-Hakeem, Maged A. F. Amine, Etemad H. Yassin, Fatma A. M. Badary

引用次数: 0

Clinicopathological features and immunophenotype of Silva pattern system in endocervical adenocarcinoma 宫颈内腺癌Silva型系统的临床病理特征及免疫表型分析

IF 3 4区医学

International Journal of Experimental Pathology Pub Date : 2023-03-16 DOI: 10.1111/iep.12470

Chao Zeng, Jin-ke Wu, Xiaofang Lu

{"title":"Clinicopathological features and immunophenotype of Silva pattern system in endocervical adenocarcinoma","authors":"Chao Zeng, Jin-ke Wu, Xiaofang Lu","doi":"10.1111/iep.12470","DOIUrl":"10.1111/iep.12470","url":null,"abstract":"The aim of this study was to investigate the correlation between Silva pattern system and clinicopathological features of endocervical adenocarcinoma. Moreover, it was to find molecular markers helpful for Silva classification, and thus we also explored the expression levels of invasion, adhesion and proliferation biomarkers in cases of Silva non-invasive and invasive types. The survival based on Silva pattern system was analysed by Kaplan–Meier survival analysis, Log-rank test and a COX risk proportionality model. Sixty samples were chosen to detect the MMP-2, MMP-9, u-PA, E-cadherin, β-catenin, EGF, TGF-α, HDGF, c-Met and RGN expression by immunohistochemistry. Multivariate analysis showed that pattern A/pattern B/pattern C Silva pattern system provided independent risk factors for prognosis. Our results found the levels of MMP-2, MMP-9 and u-PA were significantly higher in endocervical adenocarcinoma with destructive growth than in the nondestructive group. The levels of E-cadherin and β-catenin were significantly lower in endocervical adenocarcinoma with destructive growth than in the nondestructive group. The levels of EGF, TGF-α and HDGF were significantly higher in endocervical adenocarcinoma with destructive growth than in the nondestructive group. Compared with ‘non-invasive/invasive Silva pattern’, this study suggests ‘pattern A/pattern B/pattern C Silva pattern’ could be a better criteria for predicting the prognosis. Furthermore, the dual-marker combination of ‘MMP-2 and u-PA’ and ‘E-cadherin and β-catenin’ is very important in the diagnosis of Silva pattern classification.","PeriodicalId":14157,"journal":{"name":"International Journal of Experimental Pathology","volume":"104 3","pages":"140-150"},"PeriodicalIF":3.0,"publicationDate":"2023-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/iep.12470","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9474506","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

British Society for Matrix Biology Autumn Meeting 2022: “Matrix in Development” 英国基质生物学学会2022年秋季会议:“发展中的基质”

IF 3 4区医学

International Journal of Experimental Pathology Pub Date : 2023-03-12 DOI: 10.1111/iep.12471

引用次数: 0

Wnt signalling in the articular cartilage: A matter of balance 关节软骨中的Wnt信号:一个平衡问题

IF 3 4区医学

International Journal of Experimental Pathology Pub Date : 2023-02-26 DOI: 10.1111/iep.12472

Amandeep Kaur Gill, Peter J. McCormick, David Sochart, Giovanna Nalesso

引用次数: 1

MIMT1 and LINC01550 are uncharted lncRNAs down-regulated in colorectal cancer MIMT1和LINC01550是在结直肠癌中下调的未知lncrna

IF 3 4区医学

International Journal of Experimental Pathology Pub Date : 2023-02-02 DOI: 10.1111/iep.12467

Faramarz Vejdandoust, Rahmaneh Moosavi, Nasrin Fattahi Dolatabadi, Atefeh Zamani, Hossein Tabatabaeian

{"title":"MIMT1 and LINC01550 are uncharted lncRNAs down-regulated in colorectal cancer","authors":"Faramarz Vejdandoust, Rahmaneh Moosavi, Nasrin Fattahi Dolatabadi, Atefeh Zamani, Hossein Tabatabaeian","doi":"10.1111/iep.12467","DOIUrl":"10.1111/iep.12467","url":null,"abstract":"Incomplete knowledge of the molecular basis of colorectal cancer, with subsequent limitations in early diagnosis and effective treatment, has contributed to this form of malignancy becoming the second most common cause of cancer-related death worldwide. With the advances in high-throughput profiling techniques and the availability of public data sets such as The Cancer Genome Atlas Program (TCGA), a broad range of coding transcripts have been profiled and their underlying modes of action have been mapped. However, there is still a huge gap in our understanding of noncoding RNA dysregulation. To this end, we used a bioinformatics approach to shortlist and evaluate yet-to be-profiled long noncoding RNAs (lncRNAs) in colorectal cancer. We analysed the TCGA RNA-seq data and followed this by validating the expression patterns using a qPCR technique. Analysing in-house clinical samples, the real-time PCR method revealed that the shortlisted lncRNAs, that is MER1 Repeat Containing Imprinted Transcript 1 (MIMT1) and Non-Protein Coding RNA 1550 (LINC01550), were down-regulated in colorectal cancer tumours compared with the paired adjacent normal tissues. Mechanistically, the in silico results suggest that LINC01550 could form a complex competitive endogenous RNA (ceRNA) network leading to the subsequent regulation of colorectal cancer-related genes, such as CUGBP Elav-Like Family Member (CELF2), Polypyrimidine Tract Binding Protein 1 (PTBP1) and ELAV Like RNA Binding Protein 1 (ELAV1). The findings of this work indicate that MIMT1 and LINC01550 could be novel tumour suppressor genes that can be studied further to assess their roles in regulating the cancer signalling pathway(s).","PeriodicalId":14157,"journal":{"name":"International Journal of Experimental Pathology","volume":"104 3","pages":"107-116"},"PeriodicalIF":3.0,"publicationDate":"2023-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/iep.12467","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9484036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synergistic activation of genes promoting invasiveness by dual deprivation in oxygen and nutrients 通过氧气和营养双重剥夺促进侵袭性的基因协同激活

IF 3 4区医学

International Journal of Experimental Pathology Pub Date : 2023-01-24 DOI: 10.1111/iep.12464

Charly Jehanno, Yann Le Page, Gilles Flouriot, Pascale Le Goff, Denis Michel

{"title":"Synergistic activation of genes promoting invasiveness by dual deprivation in oxygen and nutrients","authors":"Charly Jehanno, Yann Le Page, Gilles Flouriot, Pascale Le Goff, Denis Michel","doi":"10.1111/iep.12464","DOIUrl":"10.1111/iep.12464","url":null,"abstract":"By depriving cancer cells of blood supplies of oxygen and nutrients, anti-angiogenic therapy is aimed at simultaneously asphyxiating and starving the cells. But in spite of its apparent logic, this strategy is generally counterproductive over the long term as the treatment seems to elicit malignancy. Since a defect of blood supply is expected to deprive tumours simultaneously of oxygen and nutrients naturally, we examine here these two deprivations, alone or in combination, on the phenotype and signalling pathways of moderately aggressive MCF7 cancer cells. Each deprivation induces some aspects of the aggressive and migratory phenotypes through activating several pathways, including HIF1-alpha as expected, but also SRF/MRTFA and TCF4/beta-catenin. Strikingly, the dual deprivation has strong cooperative effects on the upregulation of genes increasing the metastatic potential, such as four and a half LIM domains 2 (FHL2) and HIF1A-AS2 lncRNA, which have response elements for both pathways. Using anti-angiogenic agents as monotherapy is therefore questionable as it may give falsely promising short-term tumour regression, but could ultimately exacerbate aggressive phenotypes.","PeriodicalId":14157,"journal":{"name":"International Journal of Experimental Pathology","volume":"104 2","pages":"64-75"},"PeriodicalIF":3.0,"publicationDate":"2023-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/iep.12464","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9125897","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

KLF13 overexpression protects sepsis-induced myocardial injury and LPS-induced inflammation and apoptosis KLF13过表达可保护脓毒症诱导的心肌损伤和lps诱导的炎症和细胞凋亡

IF 3 4区医学

International Journal of Experimental Pathology Pub Date : 2022-12-30 DOI: 10.1111/iep.12459

Ni Zeng, Zaijin Jian, Wenxin Zhu, Junmei Xu, Yongmei Fan, Feng Xiao

{"title":"KLF13 overexpression protects sepsis-induced myocardial injury and LPS-induced inflammation and apoptosis","authors":"Ni Zeng, Zaijin Jian, Wenxin Zhu, Junmei Xu, Yongmei Fan, Feng Xiao","doi":"10.1111/iep.12459","DOIUrl":"10.1111/iep.12459","url":null,"abstract":"Sepsis remains a worldwide public health problem. This study aims to explore the role and mechanism of transcriptional factors (TFs) in sepsis-induced myocardial injury. Firstly, TF KLF13 was selected to explore its role in sepsis-induced myocardial injury. The caecal ligation and puncture (CLP) -induced sepsis mouse model was established and the septic mice were examined using standard histopathological methods. KLF13 expression was detected in the septic mouse heart and was also seen in a lipoploysaccharide (LPS) -induced cellular inflammation model. To explore this further both pro-apoptotic cleaved-caspase3/caspase3 and Bax levels and anti-apoptotic Bcl2 levels were examined, also in both models, In addition inflammatory cytokine (IL-1β, TNF-α, IL-8 and MCP-1) production and IκB-α protein level and p65 phosphorylation were examined in both septic mice and LPS-induced cells. Thus three parameters - cardiomyocyte apoptosis, inflammatory response and NF-κB pathway activation were evaluated under similar conditions. The septic mice showed significant oedema, disordered myofilament arrangement and degradation and necrosis to varying degrees in the myocardial cells. KLF13 was downregulated in both the septic mouse heart and the LPS-induced cellular inflammation model. Furthermore, both models showed abnormally increased cardiomyocyte apoptosis (increased cleaved-caspase3/caspase and Bax protein levels and decreased Bcl2 level), elevated inflammation (increased production of inflammatory cytokines) and the activated NF-κB pathway (increased p65 phosphorylation and decreased IκB-α protein level). KLF13 overexpression notably ameliorated sepsis-induced myocardial injury in vivo and in vitro. KLF13 overexpression protected against sepsis-induced myocardial injury and LPS-induced cellular inflammation and apoptosis via inhibiting the inflammatory pathways (especially NF-κB signalling) and cardiomyocyte apoptosis.","PeriodicalId":14157,"journal":{"name":"International Journal of Experimental Pathology","volume":"104 1","pages":"23-32"},"PeriodicalIF":3.0,"publicationDate":"2022-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/iep.12459","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10726587","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1