International Journal of Molecular Sciences最新文献_第2页

Bacillus subtilis and Trichoderma harzianum Reshape Rhizosphere Microbiome and Reprogram Root Transcriptome to Promote Mungbean Growth Under Continuous-Cropping Conditions. 枯草芽孢杆菌和哈茨木霉重塑根际微生物组和重编程根转录组促进绿豆连作条件下的生长

IF 4.9 2区生物学

International Journal of Molecular Sciences Pub Date : 2026-04-21 DOI: 10.3390/ijms27083699

Xinyue Liu, Yuting Chen, Xintong Zhou, Yating Xiao, Xingxing Yuan, Nana Su, Chen Chen, Qiang Yan, Xin Chen

{"title":"Bacillus subtilis and Trichoderma harzianum Reshape Rhizosphere Microbiome and Reprogram Root Transcriptome to Promote Mungbean Growth Under Continuous-Cropping Conditions.","authors":"Xinyue Liu, Yuting Chen, Xintong Zhou, Yating Xiao, Xingxing Yuan, Nana Su, Chen Chen, Qiang Yan, Xin Chen","doi":"10.3390/ijms27083699","DOIUrl":"10.3390/ijms27083699","url":null,"abstract":"Mungbean (Vigna radiata) is an important cash crop, yet the production is significantly compromised by continuous cropping. Beneficial microbial inoculation offers a promising strategy to alleviate the stresses through rhizosphere modulation and host physiological reprogramming. This study evaluated the efficacy of two biological control agents, Bacillus subtilis (B. subtilis) and Trichoderma harzianum (T. harzianum), in promoting mungbean growth under continuous-cropping conditions. Both individual applications of B. subtilis and T. harzianum significantly improved plant biomass, root system architecture, and yield. Combined metagenomic and transcriptomic analyses were conducted to unravel the underlying mechanisms. According to metagenomic analysis, both B. subtilis and T. harzianum were responsible for significant changes in beta diversity without significantly affecting the alpha diversity of the rhizosphere microbial community. T. harzianum recruited Chitinophagaceae unclassified, Abditibacterium, Hydrogenophilaceae unclassified, Methylophilaceae unclassified, and Chimaeribacter, while Bs recruited Candidatus Saccharibacteria unclassified. Transcriptomic analysis indicated that T. harzianum induced more extensive transcriptional reprogramming than B. subtilis. The enrichment analysis revealed both shared and distinct responses triggered by the two treatments. These findings suggest that B. subtilis and T. harzianum alleviate continuous-cropping stress through distinct yet complementary mechanisms involving rhizosphere microbiome modulation and mungbean transcriptional reprogramming. This study provides a sustainable strategy for legume cultivation.","PeriodicalId":14156,"journal":{"name":"International Journal of Molecular Sciences","volume":"27 8","pages":""},"PeriodicalIF":4.9,"publicationDate":"2026-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13115694/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147814830","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biodegradable Polymer-Coated Surgical Sutures for Controlled and Sustained Release of Sirolimus, Tacrolimus, and Paclitaxel. 可生物降解聚合物涂层手术缝合线用于控制和持续释放西罗莫司，他克莫司和紫杉醇。

IF 4.9 2区生物学

International Journal of Molecular Sciences Pub Date : 2026-04-21 DOI: 10.3390/ijms27083695

Joanna Chałupka, Karolina Piecyk, Karol Kurpiejewski, Adam Sikora

{"title":"Biodegradable Polymer-Coated Surgical Sutures for Controlled and Sustained Release of Sirolimus, Tacrolimus, and Paclitaxel.","authors":"Joanna Chałupka, Karolina Piecyk, Karol Kurpiejewski, Adam Sikora","doi":"10.3390/ijms27083695","DOIUrl":"10.3390/ijms27083695","url":null,"abstract":"Biodegradable polymer-coated surgical sutures represent a promising strategy for localized drug delivery to prevent post-surgical complications, such as restenosis, inflammation, and excessive tissue proliferation. In this study, bioactive coatings based on poly(L-lactic acid) (PLA), polycaprolactone (PCL), chitosan, and their binary blends were developed and applied to PLA-based surgical sutures for controlled release of sirolimus, tacrolimus, and paclitaxel. A total of 36 coated suture formulations were prepared using solvent-based deposition techniques and systematically evaluated. In vitro drug release studies conducted under physiological conditions (PBS, 37 °C) over a 12-week period demonstrated sustained and formulation-dependent release profiles. Cumulative drug release varied significantly depending on polymer composition, ranging from 17.53% to 90.93% for sirolimus, 70.93% to 98.50% for tacrolimus, and 34.62% to 67.65% for paclitaxel. PLA-based coatings generally exhibited faster release kinetics, whereas PCL-containing formulations showed slower, more sustained release. Binary polymer blends enabled fine-tuning of release profiles, demonstrating tunable drug delivery performance. All coatings maintained structural integrity during handling and simulated suturing conditions. These findings confirm that polymer composition plays a critical role in controlling drug release kinetics and demonstrate the feasibility of biodegradable polymer-coated sutures as a versatile platform for sustained, localized drug delivery in surgical and vascular applications.","PeriodicalId":14156,"journal":{"name":"International Journal of Molecular Sciences","volume":"27 8","pages":""},"PeriodicalIF":4.9,"publicationDate":"2026-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13115729/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147814955","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cerebrospinal Fluid Sediments as a Novel Tool for Potential Biomarkers of Neurodegenerative Diseases. 脑脊液沉积物作为神经退行性疾病潜在生物标志物的新工具。

IF 4.9 2区生物学

International Journal of Molecular Sciences Pub Date : 2026-04-21 DOI: 10.3390/ijms27083692

Raquel Alsina, Marta Riba, Marina Sartorio, Clara Romera, Berta Vilaplana, Eva Prats, Laura Molina-Porcel, Jaume Del Valle, Carme Pelegrí, Jordi Vilaplana

{"title":"Cerebrospinal Fluid Sediments as a Novel Tool for Potential Biomarkers of Neurodegenerative Diseases.","authors":"Raquel Alsina, Marta Riba, Marina Sartorio, Clara Romera, Berta Vilaplana, Eva Prats, Laura Molina-Porcel, Jaume Del Valle, Carme Pelegrí, Jordi Vilaplana","doi":"10.3390/ijms27083692","DOIUrl":"10.3390/ijms27083692","url":null,"abstract":"Cerebrospinal fluid (CSF) biomarkers for neurodegenerative diseases have been extensively studied over the years. However, CSF samples are routinely centrifuged, and the resulting sediment or pellet is typically discarded to remove cellular debris and high-density particles. This standard practice raises a critical question: Could these discarded sediments harbour potential biomarkers? The aim of the present study is to demonstrate that CSF sediments contain specific brain-derived components and thus to substantiate the possible presence of biomarkers within these sediments. To this end, we analysed post-mortem CSF samples of one patient with neuropathologically confirmed Alzheimer's disease (AD) and one patient with confirmed progressive supranuclear palsy (PSP). CSF pellets were studied using transmission and scanning electron microscopy techniques (TEM and SEM, respectively), along with compositional analysis through SEM combined with energy-dispersive X-ray spectroscopy (SEM-EDX), as well as immunofluorescence and histochemical analyses on semithin pellet sections. We observed that, among others, CSF pellets contain brain-derived structures such as wasteosomes and psammoma bodies. Furthermore, we also found disease-relevant proteins, including tau and Aβ42 in the AD sediment and tau in the PSP sediment. Although further studies are required, the study of CSF pellets could open new avenues for biomarker discovery in neurodegenerative diseases.","PeriodicalId":14156,"journal":{"name":"International Journal of Molecular Sciences","volume":"27 8","pages":""},"PeriodicalIF":4.9,"publicationDate":"2026-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13116889/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147815021","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integrated Bioinformatics Analysis and In Vitro Evidence Support HSP90AA1 as a Candidate Target of Camellia petelotii (Merr.) Sealy in Pulmonary Arterial Hypertension. 综合生物信息学分析及体外证据支持HSP90AA1作为油茶候选靶点的研究肺动脉高压。

IF 4.9 2区生物学

International Journal of Molecular Sciences Pub Date : 2026-04-21 DOI: 10.3390/ijms27083687

Xinying Chen, Lipeng Zhou, Chenghao Zhu, Zhirong Sun

{"title":"Integrated Bioinformatics Analysis and In Vitro Evidence Support HSP90AA1 as a Candidate Target of Camellia petelotii (Merr.) Sealy in Pulmonary Arterial Hypertension.","authors":"Xinying Chen, Lipeng Zhou, Chenghao Zhu, Zhirong Sun","doi":"10.3390/ijms27083687","DOIUrl":"10.3390/ijms27083687","url":null,"abstract":"Pulmonary arterial hypertension (PAH) is a severe and progressive cardiopulmonary disorder with limited treatment options. Camellia petelotii (Merr.) Sealy (CP) contains multiple flavonoids and other phytochemicals, but its active compounds and molecular mechanisms in PAH remain unclear. Active compounds of CP were screened by comprehensive literature mining and absorption, distribution, metabolism, and excretion (ADME) evaluation. PAH-related hub targets were identified from transcriptomic data using weighted gene co-expression network analysis (WGCNA), machine learning, and external validation. Functional enrichment, immune infiltration, and single-cell RNA-sequencing analyses were performed to characterize their biological roles and cellular localization. Molecular docking and molecular dynamics simulations assessed compound-target interactions. The effects of CP were further evaluated in hypoxia-induced rat pulmonary artery smooth muscle cells (RPASMCs). Five core bioactive compounds were identified, among which luteolin and quercetin were prioritized for further analysis. HSP90AA1 and ROCK2 were screened as hub targets. Bioinformatic analyses suggested that these targets were mainly associated with the \"Lipid and atherosclerosis\" pathway, metabolic reprogramming, and modulation of the immune microenvironment. Single-cell analysis showed broad expression of HSP90AA1 and enrichment of ROCK2 in fibroblasts and endothelial cells. Molecular docking and molecular dynamics simulations supported stable binding of luteolin to HSP90AA1. In vitro, CP extract inhibited hypoxia-induced hyperproliferation of RPASMCs and reduced HSP90AA1 protein expression. HSP90AA1 may represent a candidate molecular mediator of CP in PAH, and CP inhibited hypoxia-induced RPASMC proliferation in association with downregulation of HSP90AA1.","PeriodicalId":14156,"journal":{"name":"International Journal of Molecular Sciences","volume":"27 8","pages":""},"PeriodicalIF":4.9,"publicationDate":"2026-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13116764/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147815146","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of ANT2 as a Druggable Target for Endocrine-Resistant ERα-Positive Breast Cancer. 内分泌耐药er α阳性乳腺癌中ANT2可药物靶点的鉴定

IF 4.9 2区生物学

International Journal of Molecular Sciences Pub Date : 2026-04-21 DOI: 10.3390/ijms27083704

Erika Iguchi, Motoki Watanabe, Kaito Kobayashi, Shogen Boku, Wataru Nishio, Chikage Kato, Midori Morita, Koichi Sakaguchi, Michihiro Mutoh, Tomoshi Kameda, Yasuto Naoi

{"title":"Identification of ANT2 as a Druggable Target for Endocrine-Resistant ERα-Positive Breast Cancer.","authors":"Erika Iguchi, Motoki Watanabe, Kaito Kobayashi, Shogen Boku, Wataru Nishio, Chikage Kato, Midori Morita, Koichi Sakaguchi, Michihiro Mutoh, Tomoshi Kameda, Yasuto Naoi","doi":"10.3390/ijms27083704","DOIUrl":"10.3390/ijms27083704","url":null,"abstract":"Endocrine therapy is the mainstay for estrogen receptor (ER) α-positive breast cancer (BC), yet many patients display acquired resistance. We then screened natural compounds using human ERα-positive BC cells and identified perillyl alcohol (POH), a monoterpene from perilla, that reduces ERα protein levels. Chemoproteome analysis using POH-immobilized nanomagnetic beads revealed adenine nucleotide translocase 2 (ANT2), a mitochondrial inner membrane protein, as a direct target of POH. Molecular dynamics (MD) simulations predicted POH binding to the central pore of ANT2, which functions in ATP transport. ANT2 depletion reduced ERα levels, and public datasets indicate that high ANT2 expression correlates with poor prognosis in ERα-positive BC. POH also inhibited the growth of Tamoxifen- and Fulvestrant-resistant BC cells. RNA sequencing showed that fatty acid elongation-related genes were upregulated in Fulvestrant-resistant cells but downregulated by ANT2 depletion. Both ANT2 depletion and POH treatment led to the accumulation of intracellular lipid droplets in Fulvestrant-resistant cells, consistent with impaired fatty acid elongation. Finally, in silico screening using MD simulations identified venetoclax and nystatin as potential ANT2 pore binders. Both compounds reduced ERα levels in ERα-positive BC cells and increased lipid droplet formation in Fulvestrant-resistant cells. These findings highlight ANT2 as a druggable target against endocrine-resistant BC.","PeriodicalId":14156,"journal":{"name":"International Journal of Molecular Sciences","volume":"27 8","pages":""},"PeriodicalIF":4.9,"publicationDate":"2026-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13116172/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147814969","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Therapeutic Efficacy of Rapamycin in an Experimental Mouse Model of Corneal Alkali Burn. 雷帕霉素对实验性小鼠角膜碱烧伤模型的治疗作用。

IF 4.9 2区生物学

International Journal of Molecular Sciences Pub Date : 2026-04-21 DOI: 10.3390/ijms27083688

Basanta Bhujel, Hun Lee, Ho Seok Chung, Jae Yong Kim

{"title":"Therapeutic Efficacy of Rapamycin in an Experimental Mouse Model of Corneal Alkali Burn.","authors":"Basanta Bhujel, Hun Lee, Ho Seok Chung, Jae Yong Kim","doi":"10.3390/ijms27083688","DOIUrl":"10.3390/ijms27083688","url":null,"abstract":"Corneal alkali burn induces severe inflammation and tissue damage, leading to loss of corneal transparency and vision impairment. In this study, we evaluated the therapeutic potential of rapamycin (RAPA) compared with cyclosporine A (CsA) in a mouse model of corneal alkali burn, focusing on nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB)-mediated inflammatory signaling and its impact on corneal wound healing and repair. Notably, RAPA robustly suppressed NF-κB activation, reduced infiltration of F4/80 macrophages and MPO neutrophils, and downregulated pro-inflammatory cytokines, including TNF-α, IL-1β, and IL-6. RAPA also markedly inhibited corneal neovascularization, as evidenced by decreased VEGF expression, reduced CD31 vessel formation, and suppression of Ang-2. RAPA substantially inhibited pathological fibrotic remodeling by reducing TGF-β1 expression, attenuating myofibroblast activation (α-SMA), decreasing collagen III deposition, and modulating matrix remodeling through suppression of MMP-9. Crucially, RAPA preserved epithelial barrier integrity by maintaining occludin expression, supported proper epithelial differentiation through sustained expression of CK12, and enhanced mucin layer stability by increasing MUC1 expression. It also restored tear production, reduced apoptotic cell death (TUNEL), and decreased dysregulated epithelial proliferation (Ki67). In conclusion, RAPA showed superior efficacy compared with CsA, primarily by enhancing corneal wound healing and facilitating structural and functional outcomes in the burned cornea. These findings underscore RAPA as a promising therapeutic candidate for ocular surface repair and vision restoration in extensive corneal injury.","PeriodicalId":14156,"journal":{"name":"International Journal of Molecular Sciences","volume":"27 8","pages":""},"PeriodicalIF":4.9,"publicationDate":"2026-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13115588/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147815121","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analysis of Phosphate Transporters in Peritoneal Cells and Tissues and Their Transport Kinetics In Vitro. 腹膜细胞和组织中磷酸转运蛋白的分析及其体外转运动力学。

IF 4.9 2区生物学

International Journal of Molecular Sciences Pub Date : 2026-04-21 DOI: 10.3390/ijms27083683

Zhiwei Du, Maria Bartosova Medvid, Iva Marinovic, Sotirios G Zarogiannis, Claus Peter Schmitt

{"title":"Analysis of Phosphate Transporters in Peritoneal Cells and Tissues and Their Transport Kinetics In Vitro.","authors":"Zhiwei Du, Maria Bartosova Medvid, Iva Marinovic, Sotirios G Zarogiannis, Claus Peter Schmitt","doi":"10.3390/ijms27083683","DOIUrl":"10.3390/ijms27083683","url":null,"abstract":"Peritoneal dialysis (PD) is limited by insufficient phosphate removal, leading to adverse cardiovascular outcomes in patients with chronic kidney disease. To advance the understanding of the molecular mechanisms of peritoneal phosphate transport, RNAseq data of phosphate transporters in four PD-relevant cell lines were analyzed. The expression and localization of the respective proteins were validated by immunostaining in these cells. The transcriptomics of omental arterioles from children on PD were analyzed. In vitro Transwell models of an immortalized mesothelial cell line (MeT-5A) and human umbilical vein endothelial cells (HUVECs) and respective co-cultures were established, enabling quantification of phosphate transport across mesothelial and endothelial monolayers. Sodium phosphonoformate tribasic hexahydrate (PFA) and Tenapanor were used to inhibit transcellular and paracellular transport pathways. Cell viability and integrity markers were measured over the experimental periods. SLC20A1 and SLC20A2 were expressed across all studied cell types, while SLC34A2 and SLC34A3 were mesothelial cell-specific. Omental arterioles of children on low-glucose-degradation-product (GDP) PD showed higher SLC20A1 expression vs. stage 5 chronic kidney disease (CKD5) and healthy controls. Permeability for phosphate was lower across MeT-5A compared with HUVEC monolayers and was not further reduced in co-culture. Inhibitors reduced both transcellular and paracellular transport to 75% in MeT-5A and 65% in co-cultures, while no effects were observed in HUVEC alone, suggesting the mesothelial cell layer as a significant barrier for phosphate transport. Our studies provide first analyses combining findings on molecular phosphate transporters in peritoneal cells and arterioles and introducing a Transwell model for quantitative studies of phosphate kinetics.","PeriodicalId":14156,"journal":{"name":"International Journal of Molecular Sciences","volume":"27 8","pages":""},"PeriodicalIF":4.9,"publicationDate":"2026-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13115869/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147814738","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biomarkers and Endothelial Damage in Obesity: An Insight into the Pharmacological Modulation. 肥胖的生物标志物和内皮损伤：药理学调节的见解。

IF 4.9 2区生物学

International Journal of Molecular Sciences Pub Date : 2026-04-21 DOI: 10.3390/ijms27083694

Arturo Yonatan Bojórquez-González, Eduardo Gómez-Sánchez, Daniel Osmar Suarez-Rico, Alberto Beltrán-Ramírez, Luis Ricardo Balleza-Alejandri, Luis Daniel López-Murillo, Ernesto Javier Ramírez-Lizardo, Jesús Jonathan García-Galindo

{"title":"Biomarkers and Endothelial Damage in Obesity: An Insight into the Pharmacological Modulation.","authors":"Arturo Yonatan Bojórquez-González, Eduardo Gómez-Sánchez, Daniel Osmar Suarez-Rico, Alberto Beltrán-Ramírez, Luis Ricardo Balleza-Alejandri, Luis Daniel López-Murillo, Ernesto Javier Ramírez-Lizardo, Jesús Jonathan García-Galindo","doi":"10.3390/ijms27083694","DOIUrl":"10.3390/ijms27083694","url":null,"abstract":"Obesity drives chronic low-grade inflammation and endothelial dysfunction, key contributors to subclinical atherosclerosis. This review focuses on the netrin 1/UNC5B axis and its role in promoting macrophage retention within adipose tissue and atherosclerotic plaques, thereby perpetuating local inflammation and vascular injury. Complementary inflammatory markers-including IL 6, hsCRP, and IL 15-are discussed as indicators of systemic inflammatory burden, whereas endocan and ICAM 1 are briefly addressed as markers of endothelial activation. Among emerging pharmacological strategies, glucagon-like peptide-1 receptor agonists (GLP 1RAs) and sodium-glucose cotransporter 2 inhibitors (SGLT2is) show the most consistent evidence for improving these biomarkers and reducing endothelial damage, with GLP 1RAs demonstrating direct effects on carotid intima-media thickness. Integrating biomarker profiling with obesity phenotypes may improve early risk stratification and support more precise management of subclinical atherosclerosis.","PeriodicalId":14156,"journal":{"name":"International Journal of Molecular Sciences","volume":"27 8","pages":""},"PeriodicalIF":4.9,"publicationDate":"2026-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13116177/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147814997","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Glomerulus-Specific Inhomogeneity of the Basal Activity Map in the Olfactory Bulb. 嗅球基础活动图的肾小球特异性不均匀性。

IF 4.9 2区生物学

International Journal of Molecular Sciences Pub Date : 2026-04-21 DOI: 10.3390/ijms27083684

Stefan Fink, Natalie Fomin-Thunemann, Farzin Kamari, Yury Kovalchuk, Olga Garaschuk

引用次数: 0

miR-136-5p Preferentially Suppresses Cancer Stem-like Cells in Pancreatic Cancer. miR-136-5p在胰腺癌中优先抑制癌症干细胞样细胞。

IF 4.9 2区生物学

International Journal of Molecular Sciences Pub Date : 2026-04-21 DOI: 10.3390/ijms27083686

Hiroyuki Yamamoto, Yuhki Yokoyama, Shihori Kouda, Ruijia Yang, Yingjue Zhang, Jiaqi Wang, Yoshihiro Morimoto, Tsuyoshi Hata, Akira Inoue, Daisuke Okuzaki, Naotsugu Haraguchi, Hidekazu Takahashi, Satoshi Shibata, Hirofumi Yamamoto, Masaki Mori

{"title":"miR-136-5p Preferentially Suppresses Cancer Stem-like Cells in Pancreatic Cancer.","authors":"Hiroyuki Yamamoto, Yuhki Yokoyama, Shihori Kouda, Ruijia Yang, Yingjue Zhang, Jiaqi Wang, Yoshihiro Morimoto, Tsuyoshi Hata, Akira Inoue, Daisuke Okuzaki, Naotsugu Haraguchi, Hidekazu Takahashi, Satoshi Shibata, Hirofumi Yamamoto, Masaki Mori","doi":"10.3390/ijms27083686","DOIUrl":"10.3390/ijms27083686","url":null,"abstract":"In pancreatic cancer, cancer stem-like cells (CSCs) contribute to tumor initiation, reduced drug sensitivity, and recurrence. Limited strategies are currently available to target this cell population. Here we used a proteasome-low CSC enrichment system to identify microRNAs that negatively regulate CSC-like properties. From PANC-1 cells expressing a ZsGreen-ODC degron reporter, a proteasome-low population was isolated through sequential fluorescence-activated cell sorting of ZsGreen-positive cells. Molecular and functional analyses confirmed the CSC-like phenotype of this cell population. Integrated in silico analysis was used to select 31 microRNAs predicted to target CSC-related molecules, which were then evaluated by in vitro viability-based screening to identify candidates that selectively suppressed the viability of CSC-like cells, relative to non-CSCs. Moreover, comprehensive miRNA expression profiling revealed that miR-136-5p was downregulated in the CSC-like population and was therefore selected for further analysis. Mechanistically, miR-136-5p directly targets the 3' untranslated region of DCLK1 and reduces its expression, with a greater reduction in the short isoform. Finally, in a CSC-derived xenograft mouse model, systemic delivery of miR-136-5p using super carbonate apatite nanoparticles significantly suppressed tumor growth. Taken together, these findings suggest that miR-136-5p restoration may provide a therapeutic approach for targeting CSC-driven tumor growth in pancreatic cancer.","PeriodicalId":14156,"journal":{"name":"International Journal of Molecular Sciences","volume":"27 8","pages":""},"PeriodicalIF":4.9,"publicationDate":"2026-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13116679/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147815090","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0