International Journal of Molecular Sciences最新文献

RETRACTED: Azizi et al. Cellulose Nanocrystals/ZnO as a Bifunctional Reinforcing Nanocomposite for Poly(vinyl alcohol)/Chitosan Blend Films: Fabrication, Characterization and Properties. Int. J. Mol. Sci. 2014, 15, 11040-11053.

IF 5.6 2区生物学

International Journal of Molecular Sciences Pub Date : 2025-03-12 DOI: 10.3390/ijms26062525

Susan Azizi, Mansor B Ahmad, Nor Azowa Ibrahim, Mohd Zobir Hussein, Farideh Namvar

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Spatio-Temporal Characterization of Cellular Senescence Hallmarks in Experimental Ischemic Stroke.

IF 5.6 2区生物学

International Journal of Molecular Sciences Pub Date : 2025-03-06 DOI: 10.3390/ijms26052364

Júlia Baixauli-Martín, Maria Consuelo Burguete, Mikahela A López-Morales, María Castelló-Ruiz, Alicia Aliena-Valero, Teresa Jover-Mengual, Dianoush Falahatgaroshibi, Germán Torregrosa, Juan B Salom

{"title":"Spatio-Temporal Characterization of Cellular Senescence Hallmarks in Experimental Ischemic Stroke.","authors":"Júlia Baixauli-Martín, Maria Consuelo Burguete, Mikahela A López-Morales, María Castelló-Ruiz, Alicia Aliena-Valero, Teresa Jover-Mengual, Dianoush Falahatgaroshibi, Germán Torregrosa, Juan B Salom","doi":"10.3390/ijms26052364","DOIUrl":"10.3390/ijms26052364","url":null,"abstract":"In recent years, evidence of the existence of cellular senescence in the central nervous system has accumulated. In ischemic stroke, cellular senescence has been suggested as an unidentified pathophysiological mechanism, prompting research into the neuroprotective potential of senolytic drugs. This study aims to provide spatio-temporal evidence of the existence of brain senescence following ischemic stroke and to elucidate the involved pathways and cell types. We focused on the most established markers of senescence: cell cycle arrest (p16, p21); lysosomal activity (senescence-associated β-galactosidase [SA-β-gal]); the senescence-associated secretory phenotype ([SASP]; Interleukin-6 [IL-6], Interleukin-1β [IL-1β], Tumor necrosis factor [TNF]); and DNA/nuclear damage (Checkpoint kinase 1 [Chk1], Checkpoint kinase 2 [Chk2], Lamin B1 [LB1]). Male Wistar rats underwent 60 min of transient middle cerebral artery occlusion, followed by 24 h and 3, 7, and 14 days of recovery. Our results show significant increases in p16 expression, particularly in neurons and microglia/macrophages; SA-β-gal accumulation in the infarcted tissue; significant increases in SASP markers as early as 24 h after reperfusion; and significant changes in Chk1, Chk2, and LB1 at 14 days. Overall, our findings lend support to the existence of senescence after ischemic stroke in neurons and microglia/macrophages. However, there is still room to gain further insight into the role of senescence in the pathophysiology of ischemic stroke and in the implementation of successful senolytic therapy.","PeriodicalId":14156,"journal":{"name":"International Journal of Molecular Sciences","volume":"26 5","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11900039/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143615444","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transcriptome Analysis of Onobrychis viciifolia During Seed Germination Reveals GA₃-Inducible Genes Associated with Phenylpropanoid and Hormone Pathways.

IF 5.6 2区生物学

International Journal of Molecular Sciences Pub Date : 2025-03-06 DOI: 10.3390/ijms26052335

Yanyan Luo, Kun Wang, Jiao Cheng, Lili Nan

{"title":"Transcriptome Analysis of Onobrychis viciifolia During Seed Germination Reveals GA3-Inducible Genes Associated with Phenylpropanoid and Hormone Pathways.","authors":"Yanyan Luo, Kun Wang, Jiao Cheng, Lili Nan","doi":"10.3390/ijms26052335","DOIUrl":"10.3390/ijms26052335","url":null,"abstract":"Sainfoin (Onobrychis viciifolia) is a type of leguminous plant with high feeding value. It contains a high concentration of tannins at all growth stages, which can precipitate soluble proteins and form a large number of persistent foams in the rumen, so that ruminant livestock will not develop dilatation disease during green feeding and grazing. The germination rate of O. viciifolia seeds is very low under natural conditions. The preliminary experiment showed that 600 mg/L GA3 treatment significantly improved the germination rate and seed vitality of sainfoin seeds. In comparison to CK, GA3 significantly decreased the relative content of endogenous inhibitors, with the most notable reduction observed in 4-nitroso-N-phenyl-benzenamine. Therefore, we selected the dry seed stage (GZ), imbibition stage (XZ), split stage (LK), and radicle emergence stage (MF) of four different germination stages treated with GA3 for transcriptome analysis. RNA-seq identified 1392, 2534 and 4284 differentially expressed genes (DEGs) in GZ vs. XZ, XZ vs. LK, and LK vs. MF, respectively. During seed germination, DEGs are mainly enriched in hormone signaling and phenylalanine biosynthesis pathways, and up-down-regulation of these DEGs may alter hormone and secondary metabolite levels to promote germination. The results of weighted gene co-expression network construction (WGCNA) also indicate that plant hormone signal transduction and phenylpropanoid biosynthesis play a dominant role in GA3-induced seed germination. In conclusion, the combined analysis of transcriptomic and physiological indicators provided new insights into seed germination and a theoretical basis for further study of candidate genes.","PeriodicalId":14156,"journal":{"name":"International Journal of Molecular Sciences","volume":"26 5","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11900539/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143615476","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Reduced Expression of SATB2 in Colorectal Cancer and Its Association with Demographic and Clinicopathological Parameters.

IF 5.6 2区生物学

International Journal of Molecular Sciences Pub Date : 2025-03-06 DOI: 10.3390/ijms26052374

Anna Ewa Kowalczyk, Agnieszka Śliwińska-Jewsiewicka, Bartłomiej Emil Kraziński, Aleksandra Piotrowska, Jędrzej Grzegrzółka, Janusz Godlewski, Piotr Dzięgiel, Zbigniew Kmieć

{"title":"Reduced Expression of SATB2 in Colorectal Cancer and Its Association with Demographic and Clinicopathological Parameters.","authors":"Anna Ewa Kowalczyk, Agnieszka Śliwińska-Jewsiewicka, Bartłomiej Emil Kraziński, Aleksandra Piotrowska, Jędrzej Grzegrzółka, Janusz Godlewski, Piotr Dzięgiel, Zbigniew Kmieć","doi":"10.3390/ijms26052374","DOIUrl":"10.3390/ijms26052374","url":null,"abstract":"Special AT-rich sequence-binding protein 2 (SATB2), as a nuclear matrix-associated protein and transcription factor engaged in chromatin remodeling and the regulation of gene expression, plays an important role in growth and development processes. SATB2 has been shown to have tissue-specific expression, also related to some cancers, including colorectal cancer (CRC). The aim of this study was to compare SATB2 gene expression in tumor and matched non-involved colorectal tissues obtained from CRC patients, and to investigate its association with clinicopathological and demographic parameters, as well as patients' overall survival. SATB2 mRNA levels in the tested tissues were assessed by quantitative polymerase chain reaction, while SATB2 protein expression was determined by immunohistochemistry. We found that the average levels of both SATB2 mRNA and protein were significantly lower in tumor specimens than in matched non-involved colon tissues. Moreover, SATB2 immunoreactivity was associated with patients' sex, tumor localization, and grade of differentiation. Lower immunoreactivity of SATB2 protein was noted in high-grade tumors, in women, and in tumors located in the cecum, ascending, and transverse colon. However, the results of the present study did not show an association between SATB2 expression levels and patients' overall survival. Our findings indicate the involvement of impaired SATB2 expression, significantly reduced in high-grading tumors, in the pathogenesis of CRC, while its sex- and localization-specificity should be further elucidated.","PeriodicalId":14156,"journal":{"name":"International Journal of Molecular Sciences","volume":"26 5","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11901120/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143614961","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Resveratrol Upregulates Antioxidant Factors Expression and Downmodulates Interferon-Inducible Antiviral Factors in Aging.

IF 5.6 2区生物学

International Journal of Molecular Sciences Pub Date : 2025-03-06 DOI: 10.3390/ijms26052345

Iara Grigoletto Fernandes, Luana de M Oliveira, Milena M de Souza Andrade, Ricardo W Alberca, Júlia Cataldo Lima, Emanuella Sarmento Alho de Sousa, Anna Julia Pietrobon, Nátalli Zanete Pereira, Anna Cláudia Calvielli Castelo Branco, Alberto José da Silva Duarte, Maria Notomi Sato

{"title":"Resveratrol Upregulates Antioxidant Factors Expression and Downmodulates Interferon-Inducible Antiviral Factors in Aging.","authors":"Iara Grigoletto Fernandes, Luana de M Oliveira, Milena M de Souza Andrade, Ricardo W Alberca, Júlia Cataldo Lima, Emanuella Sarmento Alho de Sousa, Anna Julia Pietrobon, Nátalli Zanete Pereira, Anna Cláudia Calvielli Castelo Branco, Alberto José da Silva Duarte, Maria Notomi Sato","doi":"10.3390/ijms26052345","DOIUrl":"10.3390/ijms26052345","url":null,"abstract":"Immunosenescence, a process with a dysfunctional immune response that may favor infection is associated with an increase in inflammatory responses mediated by proinflammatory cytokines, characteristic of inflammaging. Aging and immunosenescence have a relationship relating to oxidative stress and inflammaging. Therefore, natural antioxidant compounds could be candidates for the control of the oxidative process. Our purpose was to evaluate the effect of resveratrol (Resv) on the antioxidant, antiviral, and anti-inflammatory responses induced by toll-like receptors (TLRs) 3, 4, and 7/8 agonists stimulation on peripheral blood mononuclear cells (PBMCs) of elderly and healthy female individuals (63-82 years old) and young and healthy female individuals (21-31 years old). Our data show that Resv may upregulate antioxidant factor expression, such as catalase (CAT) and SIRT1, in response to TLR4 and TLR7/8 agonists, similarly in both young and aged groups. Moreover, the Resv anti-inflammatory effect was detected by inhibiting IL-1β, TNF-α, and IL-10 secretion levels, as well as by the chemokines CCL2 and CCL5, induced by TLR4 and TLR7/8 stimulation. Curiously, Resv decreased antiviral genes, such as MxA, STING, and IRF7 expression, possibly by reducing the inflammatory effects of interferon-induced genes. Taken together, our results demonstrate the ability of Resv to stimulate antioxidant factors, leading to a downmodulation of the inflammatory response induced by innate immune stimulation. These findings point out Resv as a strategy to control the upregulation of inflammatory response, even in elderly individuals.","PeriodicalId":14156,"journal":{"name":"International Journal of Molecular Sciences","volume":"26 5","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11900160/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143615074","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Managing a Burning Face: Clinical Manifestations and Therapeutic Approaches for Neurogenic Rosacea.

IF 5.6 2区生物学

International Journal of Molecular Sciences Pub Date : 2025-03-06 DOI: 10.3390/ijms26052366

Gabriel Aedo, Marco Chahuán, Elsa Gatica, Isabel Herrera, Luis Felipe Parada, Alvaro Seguel, Nigel P Murray, Sócrates Aedo, Diego Aragón-Caqueo

{"title":"Managing a Burning Face: Clinical Manifestations and Therapeutic Approaches for Neurogenic Rosacea.","authors":"Gabriel Aedo, Marco Chahuán, Elsa Gatica, Isabel Herrera, Luis Felipe Parada, Alvaro Seguel, Nigel P Murray, Sócrates Aedo, Diego Aragón-Caqueo","doi":"10.3390/ijms26052366","DOIUrl":"10.3390/ijms26052366","url":null,"abstract":"Rosacea is a common chronic inflammatory condition primarily affecting middle-aged women. It presents with flushing, erythema, telangiectasia, papules, pustules, phymatous changes, and ocular involvement. Although typically grouped into four subtypes-erythematotelangiectatic, papulopustular, ocular, and phymatous-overlapping features often favor a phenotypic diagnostic approach. Neurogenic rosacea (NR) has emerged as a distinct subgroup featuring distinguishing features such as peripheral facial erythema, severe burning and stinging sensations, and resistance to standard rosacea therapies. Recent insights into the pathophysiology of NR propose neural dysregulation as the main driver of the condition. Specifically, the activation of TRP channels at cutaneous sensory nerve endings in the dermis triggers the release of vasoactive peptides, driving neuroinflammation and resulting in burning and stinging. Additionally, there is a marked association with neuropsychiatric comorbidities, which would further mediate the pathogenesis of the condition. In line with this pathophysiological model, NR often fails to respond to conventional rosacea treatments. Instead, patients benefit more from antidepressants and neuroleptic agents that help modulate neuronal activity and alleviate symptoms. This review explores and summarizes the scientific evidence regarding the new insights on disease pathogenesis, clinical manifestations, and proposed treatments for NR.","PeriodicalId":14156,"journal":{"name":"International Journal of Molecular Sciences","volume":"26 5","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11901027/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143615222","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impact of Tick-Borne Orthoflaviviruses Infection on Compact Human Brain Endothelial Barrier.

IF 5.6 2区生物学

International Journal of Molecular Sciences Pub Date : 2025-03-06 DOI: 10.3390/ijms26052342

Felix Schweitzer, Tamás Letoha, Albert Osterhaus, Chittappen Kandiyil Prajeeth

{"title":"Impact of Tick-Borne Orthoflaviviruses Infection on Compact Human Brain Endothelial Barrier.","authors":"Felix Schweitzer, Tamás Letoha, Albert Osterhaus, Chittappen Kandiyil Prajeeth","doi":"10.3390/ijms26052342","DOIUrl":"10.3390/ijms26052342","url":null,"abstract":"Tick-borne encephalitis remains a significant burden on human health in the endemic areas in Central Europe and Eastern Asia. The causative agent, tick-borne encephalitis virus (TBEV), is a neurotropic virus belonging to the genus of Orthoflavivirus. After TBEV enters the central nervous system (CNS), it mainly targets neurons, causing encephalitis and leading to life-long disabilities, coma and, in rare cases, death. The neuroinvasive mechanisms of orthoflaviviruses are poorly understood. Here we investigate the mechanism of TBEV neuroinvasion, hypothesizing that TBEV influences blood-brain barrier (BBB) properties and uses transcellular routes to cross the endothelial barrier and enter the CNS. To test this hypothesis, we employed an in vitro transwell system consisting of endothelial cell monolayers cultured on insert membranes and studied the barrier properties following inoculation to tick-borne orthoflaviviruses. It was shown that TBEV and closely related but naturally attenuated Langat virus (LGTV) crossed the intact endothelial cell monolayer without altering its barrier properties. Interestingly, transendothelial migration of TBEV was significantly affected when two cellular surface antigens, the laminin-binding protein and vimentin, were blocked with specific antibodies. Taken together, these results indicate that orthoflaviviruses use non-destructive transcellular routes through endothelial cells to establish infection within the CNS.","PeriodicalId":14156,"journal":{"name":"International Journal of Molecular Sciences","volume":"26 5","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11901142/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143615282","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Heterogeneity of the Immunological and Pathogenic Profiles in Patients Hospitalize Early Versus Late During an Acute Vital Illness as Shown in Native SARS-CoV-2 Infection.

IF 5.6 2区生物学

International Journal of Molecular Sciences Pub Date : 2025-03-06 DOI: 10.3390/ijms26052349

Krzysztof Laudanski, Ahmed Sayed Ahmed, Mohamed A Mahmoud, Mohamed Antar, Hossam Gad

{"title":"Heterogeneity of the Immunological and Pathogenic Profiles in Patients Hospitalize Early Versus Late During an Acute Vital Illness as Shown in Native SARS-CoV-2 Infection.","authors":"Krzysztof Laudanski, Ahmed Sayed Ahmed, Mohamed A Mahmoud, Mohamed Antar, Hossam Gad","doi":"10.3390/ijms26052349","DOIUrl":"10.3390/ijms26052349","url":null,"abstract":"The immune system's response to an invading pathogen is the critical determinant in recovery from illness. Here, we hypothesize that the immune response will swiftly follow classical activation and a resolution trajectory in patients with the rapid evolution of symptoms if challenged by a viral pathogen for the first time. Alternatively, a dysregulated response will be signified by a protracted clinical trajectory. Consequently, we enrolled 106 patients during the first wave of COVID-19 and collected their blood within 24 h, 48 h, 7 days, and over 28 days from symptoms onset. The pathogenic burden was measured via serum levels of the S-spike protein and specific immunoglobulin titers against the S and N proteins of SARS-CoV-2. The nonspecific immunological response was gauged using interleukin 6, leukocytosis, and C-reactive protein. Coagulation status was assessed. Several serum biomarkers were used as surrogates of clinical outcomes. We identified four clusters depending on the onset of symptoms (immediate [A], 6 days [B], 12 days [C], and over 21 days [D]). High variability in the S-spike protein in cluster A was present. The corresponding immunoglobulin titer was random. Only procalcitonin differentiated clusters in terms of markers of nonspecific inflammation. Coagulation markers were not significantly different between clusters. Serum surrogates on cardiomyopathy and neuronal pathology exhibited significant variability. Implementation of ECMO or noninvasive ventilation was more prominent in cluster C and D. Interestingly, SOFA or APACHE II scores were not different between nominal (A and B) versus dysregulated clusters (C and D).","PeriodicalId":14156,"journal":{"name":"International Journal of Molecular Sciences","volume":"26 5","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11900162/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143614965","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effectiveness of Extracellular Vesicle Application in Skin Aging Treatment and Regeneration: Do We Have Enough Evidence from Clinical Trials?

IF 5.6 2区生物学

International Journal of Molecular Sciences Pub Date : 2025-03-06 DOI: 10.3390/ijms26052354

Anna Domaszewska-Szostek, Marta Krzyżanowska, Agnieszka Polak, Monika Puzianowska-Kuźnicka

{"title":"Effectiveness of Extracellular Vesicle Application in Skin Aging Treatment and Regeneration: Do We Have Enough Evidence from Clinical Trials?","authors":"Anna Domaszewska-Szostek, Marta Krzyżanowska, Agnieszka Polak, Monika Puzianowska-Kuźnicka","doi":"10.3390/ijms26052354","DOIUrl":"10.3390/ijms26052354","url":null,"abstract":"In recent years, there has been a dynamic development in therapies utilizing extracellular vesicles (EVs) including exosomes. Therefore, we have conducted an analysis of the scientific literature to verify the current state of knowledge about these therapies. A total of 12 clinical studies were analyzed, covering the use of EVs in treating skin aging, acne scars, alopecia, and wound healing. The results indicate that EVs and exosomes hold potential in regenerative skin therapies, offering innovative and non-invasive therapeutic approaches. At the same time, significant challenges related to the standardization of their production and the lack of large-scale randomized studies were identified. Thus, we also evaluated the investigated clinical trials in regard to the MISEV (Minimal Information for Studies of Extracellular Vesicles) criteria. This review provides a comprehensive overview of the contemporary applications of EVs in skin therapy and regenerative medicine, highlighting directions for further research.","PeriodicalId":14156,"journal":{"name":"International Journal of Molecular Sciences","volume":"26 5","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11899913/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143614987","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Differentially Expressed Genes in Rat Brain Regions with Different Degrees of Ischemic Damage.

IF 5.6 2区生物学

International Journal of Molecular Sciences Pub Date : 2025-03-06 DOI: 10.3390/ijms26052347

Ivan B Filippenkov, Yana Yu Shpetko, Vasily V Stavchansky, Alina E Denisova, Vadim V Yuzhakov, Natalia K Fomina, Leonid V Gubsky, Svetlana A Limborska, Lyudmila V Dergunova

{"title":"Differentially Expressed Genes in Rat Brain Regions with Different Degrees of Ischemic Damage.","authors":"Ivan B Filippenkov, Yana Yu Shpetko, Vasily V Stavchansky, Alina E Denisova, Vadim V Yuzhakov, Natalia K Fomina, Leonid V Gubsky, Svetlana A Limborska, Lyudmila V Dergunova","doi":"10.3390/ijms26052347","DOIUrl":"10.3390/ijms26052347","url":null,"abstract":"Ischemic stroke is a multifactorial disease that leads to brain tissue damage and severe neurological deficit. Transient middle cerebral artery occlusion (tMCAO) models are actively used for the molecular, genetic study of stroke. Previously, using high-throughput RNA sequencing (RNA-Seq), we revealed 3774 differentially expressed genes (DEGs) in the penumbra-associated region of the frontal cortex (FC) of rats 24 h after applying the tMCAO model. Here, we studied the gene expression pattern in the striatum that contained an ischemic focus. Striatum samples were obtained from the same rats from which we previously obtained FC samples. Therefore, we compared DEG profiles between two rat brain tissues 24 h after tMCAO. Tissues were selected based on magnetic resonance imaging (MRI) and histological examination (HE) data. As a result, 4409 DEGs were identified 24 h after tMCAO in striatum. Among them, 2609 DEGs were overlapped in the striatum and FC, whereas more than one thousand DEGs were specific for each studied tissue. Furthermore, 54 DEGs exhibited opposite changes at the mRNA level in the two brain tissues after tMCAO. Thus, the spatial regulation of the ischemic process in the ipsilateral hemisphere of rat brain at the transcriptome level was revealed. We believe that the targeted adjustment of the genome responses identified can be the key for the induction of regeneration processes in brain cells after stroke.","PeriodicalId":14156,"journal":{"name":"International Journal of Molecular Sciences","volume":"26 5","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11900510/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143614844","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0