International Journal of Molecular Sciences最新文献

{"title":"Identification of Hub Genes and Key Pathways Associated with Sepsis Progression Using Weighted Gene Co-Expression Network Analysis and Machine Learning.","authors":"Qinghui Sun, Hai-Li Zhang, Yichao Wang, Hao Xiu, Yufei Lu, Na He, Li Yin","doi":"10.3390/ijms26094433","DOIUrl":"10.3390/ijms26094433","url":null,"abstract":"<p><p>Sepsis is a life-threatening condition driven by dysregulated immune responses, resulting in organ dysfunction and high mortality rates. Identifying key genes and pathways involved in sepsis progression is crucial for improving diagnostic and therapeutic strategies. This study analyzed transcriptomic data from 49 samples (37 septic patients across days 0, 1, and 8, and 12 healthy controls) using weighted gene co-expression network analysis (WGCNA) and multi-algorithm feature selection approaches. Differential expression analysis, pathway enrichment, and network analyses were employed to uncover potential biomarkers and molecular mechanisms. WGCNA identified modules such as MEbrown4 and MEblack, which strongly correlated with sepsis progression (r > 0.7, <i>p</i> < 0.01). Differential expression analysis highlighted up-regulated genes like CD177 and down-regulated genes like LOC440311. KEGG analysis revealed significant pathways including neuroactive ligand-receptor interaction, PI3K-Akt signaling, and MAPK signaling. Gene ontology analysis showed involvement in immune-related processes such as complement activation and antigen binding. Protein-protein interaction network analysis identified hub genes such as TNFSF10, IGLL5, BCL2L1, and SNCA. Feature selection methods (random forest, LASSO regression, SVM-RFE) consistently identified top predictors like TMCC2, TNFSF10, and PLVAP. Receiver operating characteristic (ROC) analysis demonstrated high predictive accuracy for sepsis progression, with AUC values of 0.973 (TMCC2), 0.969 (TNFSF10), and 0.897 (PLVAP). Correlation analysis linked key genes such as TNFSF10, GUCD1, and PLVAP to pathways involved in immune response, cell death, and inflammation. This integrative transcriptomic analysis identifies critical gene modules, pathways, and biomarkers associated with sepsis progression. Key genes like TNFSF10, TMCC2, and PLVAP genes show strong diagnostic potential, providing novel insights into sepsis pathogenesis and offering promising targets for future therapeutic interventions. Among these, TNFSF10 and PLVAP are known to encode secreted proteins, suggesting their potential as circulating biomarkers. This enhances their translational relevance in clinical diagnostics.</p>","PeriodicalId":14156,"journal":{"name":"International Journal of Molecular Sciences","volume":"26 9","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12072417/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143965904","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Effect of Reduced Dietary Protein on Adipose Tissue in Local Krškopolje Pigs.","authors":"Klavdija Poklukar, Marjeta Čandek-Potokar, Milka Vrecl, Jana Brankovič, Matjaž Uršič, Martin Škrlep","doi":"10.3390/ijms26094440","DOIUrl":"10.3390/ijms26094440","url":null,"abstract":"<p><p>The Slovenian autochthonous breed, Krškopolje pig, is known for high fatness and better adaptability to different environmental conditions and feed resources. However, the metabolic processes underlying these adaptations, especially in response to different diets, have not yet been studied. A deeper understanding of these mechanisms could provide valuable insights into the breed's adaptability to different environmental conditions. Therefore, the main objective of this study was to evaluate the effect of a low-protein (LP) diet on adipose tissue in Krškopolje pigs reared in either organic outdoor (n = 2 × 12) or conventional indoor (n = 2 × 14) systems. In the outdoor system, the LP diet had no effect on adipocyte size compared to the control (high-protein) diet, while it increased lipogenic enzyme activities and monounsaturated fatty acid content, and decreased polyunsaturated fatty acid content (<i>p</i> < 0.05). RNA sequencing revealed the upregulation of 28 genes and the downregulation of 37 genes. The upregulated genes were mainly involved in lipid metabolism (<i>ACLY</i>, <i>FASN</i>, <i>ACACA</i>, <i>MOGAT2</i>), oxidative stress, and mitochondrial function. In the indoor system, pigs on the LP diet had smaller adipocytes (<i>p</i> < 0.05), whereas no differences were detected in the lipogenic enzyme activities or fatty acid composition (<i>p</i> > 0.10). RNA sequencing revealed 30 upregulated and 28 downregulated genes. In the indoor system, heat shock proteins (<i>HSP70.2</i>, <i>HSPA6</i>) were upregulated in pigs on the LP diet, while genes involved in the innate immune system (<i>MSR1</i>, <i>TREM2</i>, <i>CSF3R</i>) were downregulated. To conclude, the present study showed that LP diet affected adipose tissue metabolism and gene expression in Krškopolje pigs, with different transcriptomic responses observed in outdoor and indoor rearing conditions.</p>","PeriodicalId":14156,"journal":{"name":"International Journal of Molecular Sciences","volume":"26 9","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12072434/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143965986","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolomics Combined with Photosynthetic Analysis Reveals Potential Mechanisms of Phenolic Compound Accumulation in <i>Lonicera japonica</i> Induced by Nitrate Nitrogen Supply.","authors":"Yiwen Cao, Yating Yang, Zhengwei Tan, Xihan Feng, Zhiyao Tian, Tianheng Liu, Yonghui Pan, Min Wang, Xiaoyu Su, Huizhen Liang, Shiwei Guo","doi":"10.3390/ijms26094464","DOIUrl":"10.3390/ijms26094464","url":null,"abstract":"<p><p>Mineral nutrition is of vital importance in plant growth and secondary metabolites accumulation, and thereby in the nutritional value of plants. In <i>Lonicera japonica</i>, a preference to nitrate (NO₃^--N) in comparison to ammonium (NH₄⁺-N) was found in our previous study, which can be revealed from the rapid growth rate of <i>L. japonica</i> under NO₃^--N. This study assessed whether a preference for nitrogen sources could invoke metabolic reprogramming and interrelationships between factors. NO₃^--fed plants exhibited substantial enhancement of carbon stimulation, which was strongly and positively correlated with mesophyll conductance. As a result, the elevated carbon flux by NO₃^- supplement was shuttled to phenolic metabolites synthesis, including flavones and caffeoylquinic acids compounds. Notably, the stimulation was triggered by changes in the NO₃^- and C/N ratio and was mediated by the induction of several enzymes in the phenylpropanoid pathway. On the contrary, NH₄⁺ plants showed an increment in the content of nitrogen, carbohydrates, and amino acids (mainly a strong increase in citrulline and theanine). Within secondary metabolism, NH₄⁺ may involve active lignin metabolism, showing a dramatic increment in hydroxy-ferulic acid and lignin content. This work provides significant insights regarding the mechanisms of <i>L. japonica</i> in response to diverse nitrogen regimes and effective strategies of nitrogen fertilizer input for <i>L. japonica</i>.</p>","PeriodicalId":14156,"journal":{"name":"International Journal of Molecular Sciences","volume":"26 9","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12073090/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143996696","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Non-Thermal Plasma Attenuates TNF-α-Induced Endothelial Inflammation via ROS Modulation and NF-κB Inhibition.","authors":"Joo-Hak Kim, Seonhee Kim, Shuyu Piao, Minsoo Kim, Dae-Woong Kim, Byeong Hwa Jeon, Sang-Ha Oh, Cuk-Seong Kim","doi":"10.3390/ijms26094449","DOIUrl":"10.3390/ijms26094449","url":null,"abstract":"<p><p>Non-thermal plasma (NTP) has emerged as a promising therapeutic tool due to its anti-inflammatory properties; however, its molecular effects on vascular endothelial inflammation remain unclear. This study investigated the effects of NTP on tumor necrosis factor-alpha (TNF-α)-induced inflammation in human umbilical vein endothelial cells (HUVECs). NTP treatment significantly reduced intracellular reactive oxygen species (ROS) levels and downregulated the expression of adhesion molecules such as intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1), which are key markers of endothelial activation. In addition, NTP suppressed mRNA expression of pro-inflammatory cytokines, including TNF-α, interleukin-1 beta (IL-1β), and interleukin-6 (IL-6). Mechanistically, NTP inhibited the nuclear translocation of phosphorylated NF-κB p65, indicating attenuation of NF-κB signaling. These results demonstrate that NTP modulates inflammatory responses in endothelial cells by attenuating ROS generation and suppressing NF-κB-mediated signaling. Our findings suggest that NTP may serve as a potential therapeutic strategy for treating vascular inflammation and related pathologies.</p>","PeriodicalId":14156,"journal":{"name":"International Journal of Molecular Sciences","volume":"26 9","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12073020/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143994439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic Etiology of Permanent Congenital Hypothyroidism in Korean Patients: A Whole-Exome Sequencing Study.","authors":"Jungmin Ahn, Hwalrim Jeong","doi":"10.3390/ijms26094465","DOIUrl":"10.3390/ijms26094465","url":null,"abstract":"<p><p>Congenital hypothyroidism (CH) is among the most common endocrine disorders in neonates. Genetic testing is essential for elucidating the underlying etiology, especially in cases of permanent CH. We enrolled 32 patients diagnosed with permanent CH from the Pediatric Endocrinology Clinics at Jeju National University Hospital and Soonchunhyang University Cheonan Hospital. Whole-exome sequencing (WES) was performed on genomic DNA extracted from buccal swabs. Variants were classified according to guidelines established by the American College of Medical Genetics and Genomics (ACMG) and the Association for Molecular Pathology (AMP). WES identified 21 distinct genetic variants in 20 of the 32 patients (62.5%), spanning 6 CH-related genes: <i>DUOX2</i>, <i>DUOXA2</i>, <i>TPO</i>, <i>PAX8</i>, <i>TG</i>, and <i>TSHR</i>. Of these, 12 variants classified as pathogenic or likely pathogenic were detected in 15 patients (50%). When classified by inheritance patterns, nine patients had either homozygous (n = 1) or compound heterozygous (n = 8) variants, four patients exhibited oligogenic variants, and two patients carried a single heterozygous variant with pathogenicity. The most frequently affected gene was <i>DUOX2</i>, with pathogenic or likely pathogenic variants found in six patients. Notably, none of the six patients with thyroid agenesis or ectopic thyroid glands harbored detectable pathogenic variants. Our findings underscore the critical role of genetic analysis in determining the etiology of permanent CH. Whole-exome sequencing demonstrated a high prevalence of pathogenic variants, particularly in <i>DUOX2,</i> in Korean patients with CH. These data enhance our understanding of the genetic architecture of CH and have important implications for personalized treatment and genetic counseling.</p>","PeriodicalId":14156,"journal":{"name":"International Journal of Molecular Sciences","volume":"26 9","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12072708/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144007542","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Apigenin and Luteolin on <i>Myzus persicae</i> (Hemiptera: Aphididae) Probing Behavior.","authors":"Anna Wróblewska-Kurdyk, Bożena Kordan, Jan Bocianowski, Katarzyna Stec, Beata Gabryś","doi":"10.3390/ijms26094452","DOIUrl":"10.3390/ijms26094452","url":null,"abstract":"<p><p>Apigenin and luteolin are products of the phenylpropanoid pathway, where apigenin serves as a substrate for the synthesis of luteolin. Apigenin and luteolin are highly bioactive flavones; therefore, in search of prospective biopesticides, the receptiveness of the polyphagous green peach aphid <i>Myzus persicae</i> (Sulzer) (Hemiptera: Aphididae) to apigenin and luteolin was studied. The flavones were applied as 0.1% ethanolic solutions to the host plant leaf surface, and aphid probing and feeding activities were monitored using the Electrical Penetration Graph (EPG) technique. The structural difference between apigenin and luteolin, which was the number of hydroxyl groups in the molecule, had an impact on the activity of these flavones. On apigenin-treated plants, the duration of the first probe was three times as short as on the control and five times as short as on the luteolin-treated plants; the duration of the time to the first ingestion phase within the successful probe was shorter than on the control and luteolin-treated plants; the mean duration of xylem sap ingestion bouts and the proportion of xylem phase in all probing activities were the highest; and the duration of salivation before phloem sap ingestion was the longest. Aphids' response to luteolin-treated plants was less distinct as compared to apigenin-treated plants.</p>","PeriodicalId":14156,"journal":{"name":"International Journal of Molecular Sciences","volume":"26 9","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12072685/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144008377","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"P-Type Pentatricopeptide Repeat Proteins YS1 and YS2 Function in Splicing of <i>petB</i> Intron to Maintain Chloroplast Homeostasis During Rice Seedling Development.","authors":"Hui Sun, Yanshen Nie, Li Yu, Xiaohong Yue, Xin Hou, Jie Zhao","doi":"10.3390/ijms26094459","DOIUrl":"10.3390/ijms26094459","url":null,"abstract":"<p><p>Regulating chloroplast gene expression is crucial for maintaining chloroplast function and plant development. Pentatricopeptide repeat (PPR) proteins form a vast protein family that regulates organelle genes and has multiple functions during plant development. Here, we found that two P-type PPR proteins, YS1 (yellow-green seedling 1) and YS2, jointly regulated seedling development in rice. The loss of <i>YS1</i> and <i>YS2</i> exhibited the collapsed chloroplast thylakoids and decreased photosynthetic activity, leading to the yellowing and death of rice seedlings. YS1 and YS2 could directly bind to the transcript of the <i>psbH-petB</i> intergenic region to facilitate the splicing of <i>petB</i> intron, thereby affecting the splicing efficiency of <i>petD</i>, which is located downstream of <i>petB</i> in the five-cistronic transcription unit <i>psbB-psbT-psbH-petB-petD</i>. The mutations in <i>YS1</i> and <i>YS2</i> led to decreased mature transcripts of <i>petB</i> and <i>petD</i> after splicing, significantly reducing the protein levels of PetB and PetD. This further led to deficiencies in the cytochrome b6/f and photosystem I complexes of the electron transport chain (ETC), ultimately resulting in decreased ETC-produced NADPH and reduced contents of carbohydrates in <i>ys</i> mutants. Moreover, transcriptome sequencing analysis revealed that YS1 and YS2 were vital for chloroplast organization and carbohydrate metabolism, as well as chloroplast RNA processing. In previous studies, the mechanism of <i>petB</i> intron splicing in the five-cistronic transcription unit <i>psbB-psbT-psbH-petB-petD</i> of rice is unclear. Our study revealed that the two highly conserved proteins YS1 and YS2 were functionally redundant and played critical roles in photosynthesis and seedling development through their involvement in <i>petB</i> intron splicing to maintain chloroplast homeostasis in rice. This work broadened the perspective on PPR-mediated chloroplast development and laid a foundation for exploring the biofunctions of duplicated genes in higher plants.</p>","PeriodicalId":14156,"journal":{"name":"International Journal of Molecular Sciences","volume":"26 9","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12072890/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143970219","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptomic Plasticity of Human Alveolar Macrophages Revealed by Single-Cell RNA Sequencing Following Drug Exposure: Implications for Therapeutic Development.","authors":"Penny L Groves, Levi Hockey, Brendan J O'Sullivan, Lai-Ying Zhang, Zherui Xiong, Quan H Nguyen, Maxine E Tan, Viviana P Lutzky, Rohan A Davis, Daniel C Chambers, Simon H Apte","doi":"10.3390/ijms26094439","DOIUrl":"10.3390/ijms26094439","url":null,"abstract":"<p><p>Alveolar macrophages (AM) must perform three seemingly opposing roles including homeostasis, driving inflammation, and facilitating tissue repair. Whilst there is now consensus (supported by a large body of human single cell RNA sequencing (scRNA-seq) data) that the cell subsets that perform these tasks can readily be found based on their transcriptome, their ontogeny has remained unclear. Moreover, there is agreement that in all types of pulmonary fibrosis (PF) there is an expanded population of profibrotic AM that may aberrantly drive PF. From a therapeutic viewpoint, there is great appeal in the notion that the transcriptional program in different AM subsets is not fixed but remains plastic and amenable to pharmacological reprogramming. Accordingly, this study addresses this question by performing scRNA-seq on human AM following treatment with drugs or perturbagens including pioglitazone, trametinib, nintedanib, lipopolysaccharide and the natural compound endiandrin A. Each treatment induced a unique global transcriptional change, driving the cells towards distinct subsets, further supported by trajectory analysis, confirming a high level of plasticity. Confirmatory experiments using qPCR demonstrated that single exposure to a compound induced a relatively stable transcriptome, whereas serial exposure to a different compound allowed the cells to be reprogrammed yet again to a different phenotype. These findings add new insight into the biology of AM and support the development of novel therapies to treat PF.</p>","PeriodicalId":14156,"journal":{"name":"International Journal of Molecular Sciences","volume":"26 9","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12072627/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144000380","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unraveling the Paradox of Vitamin D Status in Primary Hyperparathyroidism: An Incidental Finding or an Unexpected Consequence?","authors":"Oriana-Eliana Pelineagră, Ioana Golu, Melania Balaș, Daniela Amzăr, Iulia Plotuna, Oana Popa, Mihaela Vlad","doi":"10.3390/ijms26094434","DOIUrl":"10.3390/ijms26094434","url":null,"abstract":"<p><p>Suboptimal vitamin D status is commonly observed in primary hyperparathyroidism but is rarely considered in management decisions. The present study aimed to bring additional insights on vitamin D status in primary hyperparathyroidism patients, particularly those presenting with the normocalcemic phenotype. A retrospective study was conducted on 53 confirmed primary hyperparathyroidism patients, stratified into hypercalcemic and normocalcemic groups, hospitalized at the \"Pius Brînzeu\" Emergency Clinical Country Hospital in Timișoara, Romania. Patients presenting with the normocalcemic phenotype had similar target-organ involvement compared to their counterparts. In this subgroup, 25 hydroxyvitamin D showed an inverse correlation with serum calcium (<i>p</i> = 0.048), and regression analysis identified iPTH and 25OH vitamin D as significant predictors of calcium levels (<i>p</i> < 0.0001; R2 = 0.571). Adenoma volume showed a significant negative correlation with 25OH vitamin D levels (<i>p</i> = 0.021; r = -0.61) but was later found as insignificant after confounder analysis. Postoperative measurements of 25OH vitamin D levels confirmed increasing levels after parathyroidectomy. Our findings highlight a complex relationship between PTH and vitamin D in primary hyperparathyroidism, especially in the often-underdiagnosed normocalcemic phenotype. The inverse correlation between vitamin D and calcium suggests altered homeostasis, rather than true deficiency.</p>","PeriodicalId":14156,"journal":{"name":"International Journal of Molecular Sciences","volume":"26 9","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12072446/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143994475","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Precise Electromagnetic Modulation of the Cell Cycle and Its Applications in Cancer Therapy.","authors":"Keni Shi, Xiqing Peng, Ting Xu, Ziqi Lin, Mingyu Sun, Yiran Li, Qingyi Xian, Tingting Xiao, Siyuan Chen, Ying Xie, Ruihan Zhang, Jincheng Zeng, Bingzhe Xu","doi":"10.3390/ijms26094445","DOIUrl":"10.3390/ijms26094445","url":null,"abstract":"<p><p>Precise modulation of the cell cycle via electromagnetic (EM) control presents a groundbreaking approach for cancer therapy, especially in the development of personalized treatment strategies. EM fields can precisely regulate key cellular homeostatic mechanisms such as proliferation, apoptosis, and repair by finely tuning parameters like frequency, intensity, and duration. This review summarizes the mechanisms through which EM fields influence cancer cell dynamics, highlighting recent developments in high-throughput electromagnetic modulation platforms that facilitate precise cell cycle regulation. Additionally, the integration of electromagnetic modulation with emerging technologies such as artificial intelligence, immunotherapy, and nanotechnology is explored, collectively enhancing targeting precision, immune activation, and therapeutic efficacy. A systematic analysis of existing clinical studies indicates that EM modulation technology significantly overcomes key challenges such as tumor heterogeneity, microenvironment complexity, and treatment-related adverse effects. This review summarizes the prospects of electromagnetic modulation in clinical translation and future research directions, emphasizing its critical potential as a core element in individualized and multimodal cancer treatment strategies.</p>","PeriodicalId":14156,"journal":{"name":"International Journal of Molecular Sciences","volume":"26 9","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12072891/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143999545","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}