International Journal of Molecular Sciences最新文献_第3页

The Sequence [RRKLPVGRS] Is a Nuclear Localization Signal for Importin 8 Binding (NLS8): A Chemical Biology and Bioinformatics Study.

IF 5.6 2区生物学

International Journal of Molecular Sciences Pub Date : 2025-03-20 DOI: 10.3390/ijms26062814

Athanasios A Panagiotopoulos, Konstantina Kalyvianaki, Aikaterini Angelidaki, Dimitris Dellis, Christos A Panagiotidis, Marilena Kampa, Elias Castanas

{"title":"The Sequence [RRKLPVGRS] Is a Nuclear Localization Signal for Importin 8 Binding (NLS8): A Chemical Biology and Bioinformatics Study.","authors":"Athanasios A Panagiotopoulos, Konstantina Kalyvianaki, Aikaterini Angelidaki, Dimitris Dellis, Christos A Panagiotidis, Marilena Kampa, Elias Castanas","doi":"10.3390/ijms26062814","DOIUrl":"10.3390/ijms26062814","url":null,"abstract":"Karyopherins, carrier proteins that recognize particular cargo protein patterns known as nuclear localization signals (NLSs), mediate the nuclear translocation of big proteins. In order to better understand the process of nuclear transport of proteins and create the groundwork for the development of innovative treatments that specifically target importins, it is imperative to clarify the intricate interactions between nuclear transporters and their cargo proteins. Until recently, very few NLSs have been documented. In the current work, an in silico method was used to identify NLSs for importin 8. It was determined that the sequence RRKLPVGRS serves as a recognition motif for importin 8 binding a karyopherin that is involved in the nuclear transportation of several important proteins like AGOs, SMADs, RPL23A, and TFE3. The sequence was validated in vitro in the breast cancer cell line T47D. This work subscribes to the effort to clarify the intricate relationships between nuclear transporters and their cargo proteins, in order to better understand the mechanism of nuclear transport of proteins and lay the groundwork for the development of novel therapeutics that target particular importins and have an immediate translational impact.","PeriodicalId":14156,"journal":{"name":"International Journal of Molecular Sciences","volume":"26 6","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11942892/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143719155","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Two-Phage Cocktail Modulates Gut Microbiota Composition and Metabolic Profiles in an Ex Vivo Colon Model.

IF 5.6 2区生物学

International Journal of Molecular Sciences Pub Date : 2025-03-20 DOI: 10.3390/ijms26062805

Sthefhany Nohemí Rodríguez-Arellano, Jean Pierre González-Gómez, Bruno Gomez-Gil, Marisela González-Ávila, Juan Ramón Palomera-Hernández, Elisa Barrón-Cabrera, Marcela de Jesús Vergara-Jiménez, Cristobal Chaidez

{"title":"A Two-Phage Cocktail Modulates Gut Microbiota Composition and Metabolic Profiles in an Ex Vivo Colon Model.","authors":"Sthefhany Nohemí Rodríguez-Arellano, Jean Pierre González-Gómez, Bruno Gomez-Gil, Marisela González-Ávila, Juan Ramón Palomera-Hernández, Elisa Barrón-Cabrera, Marcela de Jesús Vergara-Jiménez, Cristobal Chaidez","doi":"10.3390/ijms26062805","DOIUrl":"10.3390/ijms26062805","url":null,"abstract":"Bacteriophage therapy is a promising approach for targeting antibiotic-resistant bacteria and modulating gut microbiota in metabolic diseases such as obesity. This study evaluated the impact of a two-phage cocktail on an ex vivo colonic simulation model of gut microbiota derived from obese individuals, both in its normalized state and after enrichment with Enterobacter cloacae, an obesity-related bacteria. Microbiological analyses confirmed that the phage cocktail remained active throughout the colonic regions over three digestion cycles and effectively reduced enterobacterial populations in the enriched microbiota. Metabarcoding of the 16S rRNA gene revealed that phage therapy did not significantly alter the abundance of dominant genera, but selectively reduced E. cloacae across all colonic regions. Alpha diversity was significantly affected only in the enriched microbiota, while beta diversity analysis indicated significant compositional shifts during therapy, with reduced dispersion in the final treatment stage. Short-chain fatty acid profiling demonstrated region- and group-specific metabolic responses, with increased lactic and butyric acid concentrations in the ascending colon of the enriched microbiota following phage treatment. This study provides the first ex vivo evidence that a two-phage cocktail can selectively eliminate E. cloacae while preserving overall microbiota structure and functionality. These findings establish a foundation for future in vivo studies exploring the role of phage therapy in reshaping gut microbial communities and metabolic profiles, highlighting its potential as a precision tool for managing gut dysbiosis in metabolic disorders.","PeriodicalId":14156,"journal":{"name":"International Journal of Molecular Sciences","volume":"26 6","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11942677/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143718824","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

5G Radiofrequency Exposure Reduces PRDM16 and C/EBP β mRNA Expression, Two Key Biomarkers for Brown Adipogenesis.

IF 5.6 2区生物学

International Journal of Molecular Sciences Pub Date : 2025-03-20 DOI: 10.3390/ijms26062792

Chandreshwar Seewooruttun, Bélir Bouguila, Aurélie Corona, Stéphane Delanaud, Raphaël Bodin, Véronique Bach, Rachel Desailloud, Amandine Pelletier

{"title":"5G Radiofrequency Exposure Reduces PRDM16 and C/EBP β mRNA Expression, Two Key Biomarkers for Brown Adipogenesis.","authors":"Chandreshwar Seewooruttun, Bélir Bouguila, Aurélie Corona, Stéphane Delanaud, Raphaël Bodin, Véronique Bach, Rachel Desailloud, Amandine Pelletier","doi":"10.3390/ijms26062792","DOIUrl":"10.3390/ijms26062792","url":null,"abstract":"The widespread use of wireless technologies has raised public health concerns about the biological effects of radiofrequency (RF) exposure. Children have a higher specific absorption rate (SAR) of radiation energy compared to adults. Furthermore, brown adipose tissue (BAT) is more prevalent in infants and tends to decrease with age. Previous animal studies demonstrated a cold sensation in rats exposed to 900 MHz (second generation, 2G). UCP1-dependent thermogenesis and BAT hyperplasia are two fundamental adaptive mechanisms initiated in response to cold. This study investigated the impact of short-term exposure to 2G and fifth generation (5G) on key thermogenic and adipogenic markers related to these mechanisms while considering age and exposure duration. Juvenile and young adult Wistar rats were randomized into three subgroups: a 5G group (3.5 GHz), 2G group (900 MHz), and a control group (SHAM). They were exposed to their respective continuous-wave RF signals for 1 or 2 weeks at an intensity of 1.5 V/m, with two exposure sessions of 1 h per day. After the exposure period, a RT-qPCR was carried out to evaluate the genetic markers involved in BAT thermogenesis and adipogenesis. Two adipogenic biomarkers were affected; a fold change reduction of 49% and 32% was detected for PRDM16 (p = 0.016) and C/EBP β (p = 0.0002), respectively, after 5G exposure, regardless of age and exposure duration. No significant RF effect was found on UCP1-dependent thermogenesis at a transcriptional level. These findings suggest that exposure to a 5G radiofrequency may partially disrupt brown adipocyte differentiation and thermogenic function by downregulating PRDM16 and C/EBP β, possibly leading to higher cold sensitivity.","PeriodicalId":14156,"journal":{"name":"International Journal of Molecular Sciences","volume":"26 6","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11942954/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143718547","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

DNA Demethylase ROS1 Interferes with DNA Methylation and Activates Stress Response Genes in Plants Infected with Beet Severe Curly Top Virus.

IF 5.6 2区生物学

International Journal of Molecular Sciences Pub Date : 2025-03-20 DOI: 10.3390/ijms26062807

Taicheng Jin, Yushuo Li, Xu Sun, Yidi Li, Zhuyi Xiao, Weiyan Wang, Jiaxue Yu, Liping Yang

{"title":"DNA Demethylase ROS1 Interferes with DNA Methylation and Activates Stress Response Genes in Plants Infected with Beet Severe Curly Top Virus.","authors":"Taicheng Jin, Yushuo Li, Xu Sun, Yidi Li, Zhuyi Xiao, Weiyan Wang, Jiaxue Yu, Liping Yang","doi":"10.3390/ijms26062807","DOIUrl":"10.3390/ijms26062807","url":null,"abstract":"DNA methylation is one mechanism of epigenetic regulation in plants. Small interfering RNAs (siRNAs) targeted endogenous genes and caused the promoters to be hypermethylated, namely RNA-directed DNA methylation (RdDM). Repressor of silencing 1 (ROS1) is an active DNA demethylase involved in the regulation of DNA methylation. This study indicates that ROS1-mediated DNA demethylation plays important roles in regulating the expression of these stress response genes and in response to biotic stresses. Further experiments confirmed that the expression level of the ROS1 gene was significantly upregulated in A. thaliana plants infected with beet severe curly top virus (BSCTV). Moreover, the DNA sequencing results demonstrated that ROS1 interferes with DNA methylation of repeat regions in the promoters of ACD6, GSTF14, and ACO3 in A. thaliana plants infected with BSCTV. These findings reveal the epigenetic mechanisms by which ROS1 regulates the expression of the stress response genes, thereby improving the adaptability of plants to biotic stresses.","PeriodicalId":14156,"journal":{"name":"International Journal of Molecular Sciences","volume":"26 6","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11943037/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143718846","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring the Potential of Optical Genome Mapping in the Diagnosis and Prognosis of Soft Tissue and Bone Tumors.

IF 5.6 2区生物学

International Journal of Molecular Sciences Pub Date : 2025-03-20 DOI: 10.3390/ijms26062820

Alejandro Berenguer-Rubio, Esperanza Such, Neus Torres Hernández, Paula González-Rojo, Álvaro Díaz-González, Gayane Avetisyan, Carolina Gil-Aparicio, Judith González-López, Nicolay Pantoja-Borja, Luis Alberto Rubio-Martínez, Soraya Hernández-Girón, María Soledad Valera-Cuesta, Cristina Ramírez-Fuentes, María Simonet-Redondo, Roberto Díaz-Beveridge, Carolina de la Calva, José Vicente Amaya-Valero, Cristina Ballester-Ibáñez, Alessandro Liquori, Francisco Giner, Empar Mayordomo-Aranda

{"title":"Exploring the Potential of Optical Genome Mapping in the Diagnosis and Prognosis of Soft Tissue and Bone Tumors.","authors":"Alejandro Berenguer-Rubio, Esperanza Such, Neus Torres Hernández, Paula González-Rojo, Álvaro Díaz-González, Gayane Avetisyan, Carolina Gil-Aparicio, Judith González-López, Nicolay Pantoja-Borja, Luis Alberto Rubio-Martínez, Soraya Hernández-Girón, María Soledad Valera-Cuesta, Cristina Ramírez-Fuentes, María Simonet-Redondo, Roberto Díaz-Beveridge, Carolina de la Calva, José Vicente Amaya-Valero, Cristina Ballester-Ibáñez, Alessandro Liquori, Francisco Giner, Empar Mayordomo-Aranda","doi":"10.3390/ijms26062820","DOIUrl":"10.3390/ijms26062820","url":null,"abstract":"Sarcomas are rare malignant tumors of mesenchymal origin with a high misdiagnosis rate due to their heterogeneity and low incidence. Conventional diagnostic techniques, such as Fluorescence In Situ Hybridization (FISH) and Next-Generation Sequencing (NGS), have limitations in detecting structural variations (SVs), copy number variations (CNVs), and predicting clinical behavior. Optical genome mapping (OGM) provides high-resolution genome-wide analysis, improving sarcoma diagnosis and prognosis assessment. This study analyzed 53 sarcoma samples using OGM. Ultra-high molecular weight (UHMW) DNA was extracted from core and resection biopsies, and data acquisition was performed with the Bionano Saphyr platform. Bioinformatic pipelines identified structural variations, comparing them with known alterations for each sarcoma subtype. OGM successfully analyzed 62.3% of samples. Diagnostic-defining alterations were found in 95.2% of cases, refining diagnoses and revealing novel oncogenic and tumor suppressor gene alterations. The challenges included DNA extraction and quality issues from some tissue samples. Despite these limitations, OGM proved to be a powerful diagnostic and predictive tool for bone and soft tissue sarcomas, surpassing conventional methods in resolution and scope, enhancing the understanding of sarcoma genetics, and enabling better patient stratification and personalized therapies.","PeriodicalId":14156,"journal":{"name":"International Journal of Molecular Sciences","volume":"26 6","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11942867/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143718991","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lifestyle and Environmental Factors Affecting Male Fertility, Individual Predisposition, Prevention, and Intervention.

IF 5.6 2区生物学

International Journal of Molecular Sciences Pub Date : 2025-03-20 DOI: 10.3390/ijms26062797

Jan Tesarik

{"title":"Lifestyle and Environmental Factors Affecting Male Fertility, Individual Predisposition, Prevention, and Intervention.","authors":"Jan Tesarik","doi":"10.3390/ijms26062797","DOIUrl":"10.3390/ijms26062797","url":null,"abstract":"Current lifestyles bring about an increasing prevalence of unhealthy habits that can negatively affect male fertility. Cigarette smoking, alcohol intake, stress, inadequate physical activity, an unequilibrated diet leading to obesity, and use of mobile telephones and portable electronic devices can affect the male reproductive system through multiple mechanisms. Moreover, the modern man is often exposed to environmental factors independent of his will, such as air pollution, exposure to heat or toxicants in his workplace, or the presence of harmful chemicals in food, beverages, agricultural and industrial products, etc. The susceptibility to these factors depends on genetic and epigenetic predisposition, potentially present systemic disease and medication, and local affections of the genitourinary system. The multifaceted nature of both the causative factors and the susceptibility background makes the resulting fertility disturbance highly individual and variable among different men exposed to the same conditions. This paper critically reviews the current knowledge of different causative and susceptibility factors with a special attention to the molecular mechanisms of their action. Finally, strategies for the prevention of abnormalities due to lifestyle and environmental factors and available treatment modalities for already-present abnormalities are exposed.","PeriodicalId":14156,"journal":{"name":"International Journal of Molecular Sciences","volume":"26 6","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11943017/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143718969","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification and Assessment of lncRNAs and mRNAs in PM2.5-Induced Hepatic Steatosis.

IF 5.6 2区生物学

International Journal of Molecular Sciences Pub Date : 2025-03-20 DOI: 10.3390/ijms26062808

Peixuan Tian, Hui Xia, Xinbao Li, Ying Wang, Bihuan Hu, Yu Yang, Guiju Sun, Jing Sui

{"title":"Identification and Assessment of lncRNAs and mRNAs in PM2.5-Induced Hepatic Steatosis.","authors":"Peixuan Tian, Hui Xia, Xinbao Li, Ying Wang, Bihuan Hu, Yu Yang, Guiju Sun, Jing Sui","doi":"10.3390/ijms26062808","DOIUrl":"10.3390/ijms26062808","url":null,"abstract":"Research indicates that fine particulate matter (PM2.5) exposure is associated with the onset of non-alcoholic fatty liver disease (NAFLD), the most prevalent chronic liver disorder. However, the underlying pathogenesis mechanisms remain to be fully understood. Our study investigated the hub long non-coding RNAs (lncRNAs) and messenger RNAs (mRNAs) associated with hepatic steatosis caused by PM2.5 exposure and their pathological mechanisms. The analysis of gene profiles in the GSE186900 dataset from the Gene Expression Omnibus (GEO) enabled the identification of 38 differentially expressed lncRNAs and 1945 mRNAs. To explore further, a co-expression network was established utilizing weighted gene co-expression network analysis (WGCNA). Moreover, Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) databases were utilized for functional enrichment analysis. Our analysis identified specific modules, particularly the blue and turquoise modules, which showed a strong correlation with NAFLD. Through functional enrichment analysis, we identified several lncRNAs (including Gm15446, Tmem181b-ps, Adh6-ps1, Gm5848, Zfp141, Rmrp, and Rb1) which may be involved in modulating NAFLD, multiple metabolic pathways, inflammation, cell senescence, apoptosis, oxidative stress, and various signaling pathways. The hub lncRNAs identified in our study provide novel biomarkers and potential targets for the diagnosis and treatment of NAFLD.","PeriodicalId":14156,"journal":{"name":"International Journal of Molecular Sciences","volume":"26 6","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11943408/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143718738","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Extracellular Vesicles in the Mesenchymal Stem Cell/Macrophage Axis: Potential Targets for Inflammatory Treatment.

IF 5.6 2区生物学

International Journal of Molecular Sciences Pub Date : 2025-03-20 DOI: 10.3390/ijms26062827

Zhen Che, Wenbin Yan, Qun Zhao

{"title":"Extracellular Vesicles in the Mesenchymal Stem Cell/Macrophage Axis: Potential Targets for Inflammatory Treatment.","authors":"Zhen Che, Wenbin Yan, Qun Zhao","doi":"10.3390/ijms26062827","DOIUrl":"10.3390/ijms26062827","url":null,"abstract":"Mesenchymal stem cells (MSCs) have been widely used for the treatment of autoimmune and inflammatory diseases due to their pluripotent differentiation potential and immunomodulatory function. Macrophage (Mφ) polarization also acts an essential and central role in regulating inflammation, basically the dynamic balance of pro-inflammatory M1-like (M1φ) and anti-inflammatory M2-like macrophages (M2φ), affecting the occurrence and progression of inflammatory diseases. Since a pivotal molecular crosstalk between MSCs and Mφ has been elucidated using in vitro and in vivo preclinical studies, we presume that the mesenchymal stem cell/macrophages axis (MSC/Mφ axis) acts an important role in pathophysiological mechanisms of inflammatory diseases and should be the potential therapeutic target. However, the crucial effects of EVs as intercellular communicators and therapeutic agents in the MSC/Mφ axis remains explorable. Therefore, this review elaborated on the mechanisms of EVs mediating the MSC/Mφ axis regulating inflammation in-depth, hoping to provide more references for related research in the future.","PeriodicalId":14156,"journal":{"name":"International Journal of Molecular Sciences","volume":"26 6","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11943156/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143718997","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

GPCR Sense Communication Among Interaction Nematodes with Other Organisms.

IF 5.6 2区生物学

International Journal of Molecular Sciences Pub Date : 2025-03-20 DOI: 10.3390/ijms26062822

Jie Wang, Changying Guo, Xiaoli Wei, Xiaojian Pu, Yuanyuan Zhao, Chengti Xu, Wei Wang

{"title":"GPCR Sense Communication Among Interaction Nematodes with Other Organisms.","authors":"Jie Wang, Changying Guo, Xiaoli Wei, Xiaojian Pu, Yuanyuan Zhao, Chengti Xu, Wei Wang","doi":"10.3390/ijms26062822","DOIUrl":"10.3390/ijms26062822","url":null,"abstract":"Interactions between species give rise to chemical pathways of communication that regulate the interactions of transboundary species. The communication between nematodes and other species primarily occurs through the regulation of chemicals, with key species including plants, insects, bacteria, and nematode-trapping fungi that are closely associated with nematodes. G protein-coupled receptors (GPCRs) play a crucial role in interspecies communication. Certain flp genes, which function as GPCRs, exert varying degrees of influence on how nematodes interact with other species. These receptors facilitate the transmission of corresponding signals, thereby completing the interactions between species. This paper introduces the interactions between nematodes and other species and discusses the role of GPCRs in these organisms, contributing to a deeper understanding of the impact and significance of GPCRs in cross-border regulation between nematodes and other species. Furthermore, it is essential to leverage GPCRs in efforts to control pests.","PeriodicalId":14156,"journal":{"name":"International Journal of Molecular Sciences","volume":"26 6","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11943259/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143719033","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

NCKAP1 Inhibits the Progression of Renal Carcinoma via Modulating Immune Responses and the PI3K/AKT/mTOR Signaling Pathway.

IF 5.6 2区生物学

International Journal of Molecular Sciences Pub Date : 2025-03-20 DOI: 10.3390/ijms26062813

Xin Zhang, Jianqing Ye, Lixiang Sun, Wanli Xu, Xiaomeng He, Juan Bao, Jin Wang

{"title":"NCKAP1 Inhibits the Progression of Renal Carcinoma via Modulating Immune Responses and the PI3K/AKT/mTOR Signaling Pathway.","authors":"Xin Zhang, Jianqing Ye, Lixiang Sun, Wanli Xu, Xiaomeng He, Juan Bao, Jin Wang","doi":"10.3390/ijms26062813","DOIUrl":"10.3390/ijms26062813","url":null,"abstract":"Nck-associated protein 1 (NCKAP1) is critical for cytoskeletal functions and various cellular activities, and deregulation of NCKAP1 in many cancers significantly influences the outcomes of malignant diseases. However, the functions of NCKAP1 in the progression of renal cancer are yet unknown. To investigate the specific roles of NCKAP1 in the immune regulation and tumor progression of renal cancer, the expression of NCKAP1 and genetic variations were analyzed across cancer types at different pathological stages via UALCAN and cBioPortal. Immune cell infiltration in renal cancer was also assessed by ssGSEA and single-cell gene expression data from the GEO. RNA sequencing of NCKAP1-overexpressing 769P cells further examined the impact of NCKAP1 on kidney cancer. Our pancancer analyses revealed a complex NCKAP1 expression profile across various cancer types, with reduced levels in renal cancer patients linked to patient prognosis. CIBERSORT and single-cell RNA sequencing revealed the expression patterns of NCKAP1 in different cell lineages in renal cancer and a significant correlation between NCKAP1 and immune cell infiltration in the kidney tumor microenvironment. We further verified that NCKAP1 suppressed cancer cell growth and affected tumor development in renal cancer via the PI3K/AKT/mTOR signaling pathway. Our results indicate that NCKAP1 is a potential predictive marker and treatment target for renal cancer.","PeriodicalId":14156,"journal":{"name":"International Journal of Molecular Sciences","volume":"26 6","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11942877/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143718869","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0