Karina Ilyasova, Elena Zerkalenkova, Olga Soldatkina, Anna Kazakova, Natalya Myakova, Julia Roumiantseva, Veronica Fomynih, Alexander Popov, Grigory Tsaur, Yulia Olshanskaya, Michael Maschan
{"title":"Genetic Diversity in KMT2A-r and KMT2A-Wt Groups: Assessing the Prognostic Value of Markers in BCP-ALL Among Infants","authors":"Karina Ilyasova, Elena Zerkalenkova, Olga Soldatkina, Anna Kazakova, Natalya Myakova, Julia Roumiantseva, Veronica Fomynih, Alexander Popov, Grigory Tsaur, Yulia Olshanskaya, Michael Maschan","doi":"10.1111/ijlh.14442","DOIUrl":"10.1111/ijlh.14442","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background/Objectives</h3>\u0000 \u0000 <p>Infant BCP-ALL is classified into <i>KMT2A</i>-r and <i>KMT2A</i>-wt groups, both showing heterogeneity. <i>KMT2A</i> rearrangements indicate poor prognosis, but outcomes vary by fusion partner. The <i>KMT2A</i>-wt group includes cases in the B-other ALL subgroup, with unclear prognostic significance. We aim to improve understanding of molecular subtypes in <i>KMT2A</i>-r and <i>KMT2A</i>-wt, focusing on <i>NUTM1</i> and <i>PAX5</i> rearrangements.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>We analyzed 175 infants (aged 0–365 days) diagnosed with BCP-ALL from 2010 to 2023 at the Dmitry Rogachev National Medical Research Center of Pediatric Hematology, Oncology and Immunology. Genomic aberrations were identified by karyotyping, FISH and RNA-seq. RNA-seq was performed using the Illumina, and gene fusions were validated by Sanger sequencing.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>There was no difference in survival based on <i>KMT2A</i> partner genes. The <i>KMT2A::AFF1</i> group showed similar outcomes to other partners, with 2-year EFS of 36% (95% CI, 21%–59%) versus 37% (95% CI, 23%–60%) (log-rank test, <i>p</i> = 0.9). In the <i>KMT2A</i>-wt group (<i>n</i> = 33, 17.7% of cases), <i>NUTM1-r</i> (<i>n</i> = 9) and <i>PAX5-r</i> (<i>n</i> = 10) accounted for 27% and 30.3%, respectively. The <i>NUTM1</i>-r and <i>PAX5</i>-r groups showed excellent survival rates, with 2-year EFS of 80% (95% CI, 52%–100%) and 100% (95% CI, 100%–100%), respectively, but the small cohort size limit the statistical power of the analysis (log-rank test, <i>p</i> = 0.9).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>Survival in the <i>KMT2A</i>-r group did not differ by fusion partner. <i>NUTM1</i> rearrangements showed a favorable prognosis, and <i>PAX5</i>-rearranged patients had better outcomes than previously reported. In the <i>NUTM1</i>-r group, the most common fusion, <i>BRD9:NUTM1</i>, showed variability in breakpoints (Exons 3, 8, and 14 of <i>BRD9</i>).</p>\u0000 </section>\u0000 </div>","PeriodicalId":14120,"journal":{"name":"International Journal of Laboratory Hematology","volume":"47 3","pages":"472-480"},"PeriodicalIF":2.2,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143506656","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Overexpression of lncRNAs HOTAIR and MALAT1 While Downexpression of lncRNA PANDA Predict the Resistance of Diffuse Large B-Cell Lymphoma to R-CHOP Chemoimmunotherapy","authors":"Yara Mohamed Ahmed, Nashwa El-Khazragy, Riham Abdel-Hamid Haroun, Shadia Abdel-Hamid Fathy","doi":"10.1111/ijlh.14440","DOIUrl":"10.1111/ijlh.14440","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Long noncoding RNAs (lncRNAs) have emerged as key regulators of cancer; in addition, they have been identified as novel therapeutic targets and biomarkers for several cancers, including diffuse large B-cell lymphoma (DLBCL).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>A total of 75 DLBCL patients and 30 control subjects with active lymph nodes were enrolled into our study. The baseline expression levels of lncRNAs HOTAIR, MALAT1, and PANDA in paraffin-embedded blocks of lymph nodes from DLBCL patients and controls were evaluated using reverse transcription quantitative real-time PCR (RTqPCR) technique.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>The expression levels of HOTAIR and MALAT1 were increased while PANDA expression level was decreased in DLBCL patients when compared to controls and in R-CHOP-resistant patients when compared to responder ones. Also, HOTAIR, MALAT1, and PANDA baseline expression levels were significantly correlated with the different clinical parameters in this study. As diagnostic and prognostic tools, the results obtained from the ROC curve revealed that the PANDA baseline expression level was the best one as the diagnostic biomarker could differentiate DLBCL disease and the prognostic biomarker predicts R-CHOP resistance.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>In conclusion, the integrated approach reveals that lncRNAs HOTAIR and MALAT1 were upregulated, while lncRNA PANDA was downregulated in DLBCL patients compared with controls, and the three lncRNAs closely associated with clinical prognosis. This study warrants future studies in clinical trials for the treatment of R-CHOP-resistant DLBCL patients.</p>\u0000 </section>\u0000 </div>","PeriodicalId":14120,"journal":{"name":"International Journal of Laboratory Hematology","volume":"47 3","pages":"463-471"},"PeriodicalIF":2.2,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143506658","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Lilan Jin, Lei Li, Yiyi Lu, Gang Cai, Lin Lin, Jiafei Lin
{"title":"Development and Validation of a User-Friendly Predictive Model Using Demographic and Complete Blood Count Data to Facilitate Early Diagnosis on Suspicion of Myeloproliferative Neoplasms","authors":"Lilan Jin, Lei Li, Yiyi Lu, Gang Cai, Lin Lin, Jiafei Lin","doi":"10.1111/ijlh.14452","DOIUrl":"10.1111/ijlh.14452","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objective</h3>\u0000 \u0000 <p>To develop a novel predictive model based on demographics and complete blood count (CBC) parameters to quickly identify suspicious features of myeloproliferative neoplasms (MPN), enabling prompt initiation of further investigations and referrals.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>426 patients with elevated peripheral blood cell counts were referred to the Hematology Department of Ruijin Hospital from 2017 to 2023. Among them, 215 patients were diagnosed with MPN, while the remaining 211 patients formed the non-MPN group. The patients were randomly divided into a training cohort and a validation cohort. Demographic characteristics, CBC data, and other relevant laboratory information were collected. By univariable and multivariable logistic regression, significant indicators independently associated with MPN were identified and included in the nomogram. The model was evaluated by measuring the area under the receiver operating characteristic curve (AUC), calibration curve, and decision curve analysis (DCA) curve.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Five indicators were identified as independently associated with MPN, including onset age, monocyte fraction, basophil fraction, red blood cell distribution width, and platelet count. The AUC values for the training and validation cohorts were 0.912 and 0.928, respectively. The calibration curves showed good agreement between the predicted risk by the nomogram and the actual outcomes. The DCA for the training and the validation datasets revealed net benefits of 0.9026 and 0.9303, respectively.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>We have developed and validated a prediction model for MPN based on demographics and CBC data. The model could assist general practitioners in quickly identifying patients with potential MPN and in initiating timely further investigations and referrals.</p>\u0000 </section>\u0000 </div>","PeriodicalId":14120,"journal":{"name":"International Journal of Laboratory Hematology","volume":"47 4","pages":"651-659"},"PeriodicalIF":2.2,"publicationDate":"2025-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143477011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Mazen Assar, Henning Nilius, Natalie Kearn, Wilma Hopman, Michael Nagler, Maha Othman
{"title":"Thromboelastometry (ROTEM) Assessing Hypercoagulability in Patients Referred for Thrombophilia Screening","authors":"Mazen Assar, Henning Nilius, Natalie Kearn, Wilma Hopman, Michael Nagler, Maha Othman","doi":"10.1111/ijlh.14443","DOIUrl":"10.1111/ijlh.14443","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Introduction</h3>\u0000 \u0000 <p>Thrombophilia, a blood coagulation disorder, poses risks of venous thromboembolism (VTE). Coagulation assays may not be sufficient to assess VTE risk and global assays such as Rotational Thromboelastometry (ROTEM) may add valuable information. We investigated ROTEM's capacity to detect hypercoagulability in patients undergoing thrombophilia screening, its potential impact on patient outcomes, and limitations.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Comprehensive clinical, laboratory, genetic tests, and ROTEM (EXTEM and INTEM) were conducted for 356 patients referred for thrombophilia screening at an academic hospital outpatient unit. Hypercoagulability was identified as a shorter clot formation time (CFT), larger alpha angle (AA), and greater maximum clot firmness (MCF), and was compared in patients with and without VTE. Statistically this was analyzed using Mann–Whitney <i>U</i> and Chi-square tests with <i>p</i> < 0.05 considered significant.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Among 356 patients, 64.6% had previous VTE, with 76.9% experiencing one event, 14.3% recurrent (35.6% unprovoked, 64.4% provoked). 22.5% of patients were on anticoagulation. Those with VTE history exhibited significant alterations in EXTEM and INTEM parameters compared to those without (<i>p</i> < 0.001), showing decreased CFT and increased AA and MCF. However, receiver operating characteristic curves for these variables indicated that none were able to discriminate between those individuals with and without thromboembolic complications.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>ROTEM does not appear to be a strong discriminatory test. However, it can detect hypercoagulopathy in patients referred for thrombophilia screening. Abnormal ROTEM may indicate a higher risk for recurrence. However, this can only be determined in prospective cohort studies.</p>\u0000 </section>\u0000 </div>","PeriodicalId":14120,"journal":{"name":"International Journal of Laboratory Hematology","volume":"47 3","pages":"520-528"},"PeriodicalIF":2.2,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/ijlh.14443","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143470433","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Dysfibrinogenemia With the γSer358Cys Variant, Fibrinogen Milano VII, Escapes From the Clauss-CWA Classification","authors":"Atsuo Suzuki, Nobuaki Suzuki, Shogo Tamura, Shuichi Okamoto, Takeshi Kanematsu, Tetsuhito Kojima, Tadashi Matsushita","doi":"10.1111/ijlh.14451","DOIUrl":"10.1111/ijlh.14451","url":null,"abstract":"","PeriodicalId":14120,"journal":{"name":"International Journal of Laboratory Hematology","volume":"47 3","pages":"559-562"},"PeriodicalIF":2.2,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143461213","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Emmanuel J. Favaloro, Sandya Arunachalam, Elysse Dean, Mahzuza Salwa, Monica Ahuja, Lynne Connelly, Kent Chapman, Ronny Vong, Leonardo Pasalic
{"title":"Activated Protein C Resistance Testing: An Update From Australasia/Asia-Pacific","authors":"Emmanuel J. Favaloro, Sandya Arunachalam, Elysse Dean, Mahzuza Salwa, Monica Ahuja, Lynne Connelly, Kent Chapman, Ronny Vong, Leonardo Pasalic","doi":"10.1111/ijlh.14447","DOIUrl":"10.1111/ijlh.14447","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Introduction</h3>\u0000 \u0000 <p>Activated protein C resistance (APCR) represents a risk factor for thrombosis and is usually due to factor V Leiden (FVL). Clinicians may order either test (i.e., APCR or FVL) to help assess ‘thrombophilia’ in patients who present with thrombosis. APCR testing is usually achieved using clot-based assays, whereas FVL is assessed by genetic testing. There are advantages and disadvantages to either approach.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>We report updated findings for APCR testing in our geographic region, in part using recent data from the RCPAQAP, an international external quality assessment (EQA) program, with some 50–60 participants for APCR testing over the past decade. Data have been updated to cover the past 13 years (2010–2023 inclusive), with four samples assessed each year, but with a primary focus on new data from 2020 to 2023 inclusive. In addition, data for APCR testing over several years from four large tertiary-level hospital laboratories have been assessed following a recent change in instrumentation and haemostasis methods.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>EQA data continue to show variable performance in both numerical values and their interpretation for APCR testing, with certain methods providing more consistently correct findings than others. In addition, participant interpretation of their own numerical values and transcription errors seem problematic. Finally, the change in recent laboratory testing has also evidenced local improvements.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>APCR assays and testing laboratories continue to show variability in performance, with two methods (Pefakit and Staclot) showing the best performance overall. Targeted education may be of benefit, as most of the errors appear to originate from a small proportion of laboratories.</p>\u0000 </section>\u0000 </div>","PeriodicalId":14120,"journal":{"name":"International Journal of Laboratory Hematology","volume":"47 4","pages":"720-729"},"PeriodicalIF":2.2,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/ijlh.14447","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143461206","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Manish K. Singh, Arun S. Nair, Akshita Pandey, Vineet Sharma, Khaliqur Rahman, Ruchi Gupta, Dinesh Chandra, Sanjeev Yadav, Rajesh Kashyap, S. R. Arun, Mayur Parihar
{"title":"A Novel 5-Probe FISH Strategy is Better Equipped for a More Comprehensive and Cost-Effective Risk Stratification of BCP-ALL","authors":"Manish K. Singh, Arun S. Nair, Akshita Pandey, Vineet Sharma, Khaliqur Rahman, Ruchi Gupta, Dinesh Chandra, Sanjeev Yadav, Rajesh Kashyap, S. R. Arun, Mayur Parihar","doi":"10.1111/ijlh.14441","DOIUrl":"10.1111/ijlh.14441","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objective</h3>\u0000 \u0000 <p>The modern treatment protocols in B-cell precursor acute lymphoblastic leukemia (BCP-ALL) are based on the disease's genetic characteristics and response to treatment. We propose a novel five-probe FISH strategy to risk stratify the BCP-ALL and compare its ability with the triple trisomy probe strategy to detect high hyperdiploidy.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>All newly diagnosed BCP-ALL cases were investigated using a five-probe FISH panel that included probes targeting <i>BCR::ABL1</i> fusion, <i>ETV6::RUNX1</i> fusion, and break-apart probes for <i>KMT2A</i>, <i>IgH</i>, and <i>CRLF2</i> rearrangements. Further, a selected number of cases were screened by the triple trisomy probe of 4p11/CEN10/17 (Zytovision, Bremerhaven, Germany) to identify aneuploidy.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Of the 380 patients of BCP-ALL screened (≤ 18 years: 57.9%; > 18 years: 42.1%) using this five-probe strategy, we could assign clinically relevant eight risk groups to almost two-thirds of the patients (similar to the available literature). Compared with the widely accepted triple trisomy probe strategy, we found concordant findings in 75.5% of the patients; the triple trisomy probe could not identify high hyperdiploidy in 24.5% of patients. We observed the presence of (non-<i>CRLF2</i>) <i>IgH</i> rearrangement in 5.3% of patients.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>We conclude that the proposed five-probe FISH strategy is better equipped to more comprehensively risk stratify BCP-ALL patients, with an increased ability to identify high hyperdiploidy and a subset of Ph-like-BCP-ALL.</p>\u0000 </section>\u0000 </div>","PeriodicalId":14120,"journal":{"name":"International Journal of Laboratory Hematology","volume":"47 3","pages":"437-444"},"PeriodicalIF":2.2,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143443106","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Comment on “Platelet Reactivity to Zika and Dengue Non-Structural Protein 1 (NS1) Assessed by Flow Cytometry, Atomic Force Microscopy, and Quartz Crystal Microbalance”","authors":"Hinpetch Daungsupawong, Viroj Wiwanitkit","doi":"10.1111/ijlh.14439","DOIUrl":"10.1111/ijlh.14439","url":null,"abstract":"","PeriodicalId":14120,"journal":{"name":"International Journal of Laboratory Hematology","volume":"47 3","pages":"557-558"},"PeriodicalIF":2.2,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143401049","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Idiopathic Mild Platelet Dysfunction: Baseline Characteristics and Clinical Courses","authors":"Nitchkan Wiwatsomwong, Ratchaneekorn Jantasing, Benjaporn Akkawat, Noppacharn Uapresert, Ponlapat Rojnuckarin","doi":"10.1111/ijlh.14433","DOIUrl":"10.1111/ijlh.14433","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Introduction</h3>\u0000 \u0000 <p>The causes of nonsyndromic platelet storage pool disease are still unclear, and whether they are of genetic or acquired origin remains to be defined. The study aimed to describe the characteristics and natural history of this disorder.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>This mostly retrospective cohort enrolled adults presenting with bleeding from platelet dysfunction. Platelet glycoprotein defects, von Willebrand disease, syndromic inherited platelet disorders and known acquired platelet dysfunctions were excluded. Available patients were retested by lumiaggregometry (Chrono-Log) over 1 year after the initial diagnosis.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>There was a total of 56 patients; 91% female, with a median diagnostic age of 28 years (interquartile range [IQR]: 24.5–38.5). The subnormal responses to ADP, epinephrine, collagen, and arachidonate were found in 91%, 82%, 55%, and 34%, respectively. Nineteen patients had von Willebrand factor levels measured. Twenty-three subjects underwent repeat tests. Twenty-one of them were female (91%), with a median age and follow-up time of 37 years (IQR: 28–55) and 6 years (IQR: 3–12), respectively. Median ISTH-BAT bleeding scores at diagnosis and follow-up were 5 (IQR: 3–8) and 1 (IQR: 0–2), respectively. The common abnormalities were reduced responses to ADP combined with other agonists (83%). Twelve (52%) and five (22%) showed complete and partial platelet function recovery, respectively. None of the partial and non-recovery groups had a bleeding score over 4 at follow-up.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>Idiopathic mild platelet dysfunction was female-predominant and showed spontaneous symptom resolution after a long follow-up. Platelet function recovery was observed in most cases. Exogenous factors triggering this condition remain to be identified.</p>\u0000 </section>\u0000 </div>","PeriodicalId":14120,"journal":{"name":"International Journal of Laboratory Hematology","volume":"47 3","pages":"503-509"},"PeriodicalIF":2.2,"publicationDate":"2025-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143257644","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}