International Journal of Laboratory Hematology最新文献

{"title":"Evaluation of Coagulation and Fibrinolysis Mechanisms in Deep Vein Thrombosis Using Clot-Fibrinolysis Waveform Analysis","authors":"Akiharu Yoshioka,&nbsp;Daiki Shimomura,&nbsp;Mayuka Morita,&nbsp;Aya Kono,&nbsp;Tomoko Matsumoto,&nbsp;Hirokazu Kondo,&nbsp;Masashi Shimada,&nbsp;Mikio Kamioka,&nbsp;Osamu Kumano","doi":"10.1111/ijlh.14437","DOIUrl":"10.1111/ijlh.14437","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Introduction</h3>\u0000 \u0000 <p>Deep vein thrombosis (DVT) is a common condition associated with significant morbidity and mortality. However, the detailed mechanisms of coagulation and fibrinolysis in DVT have not been adequately investigated. Recently, clot-fibrinolysis waveform analysis (CFWA) has been developed to assess coagulation and fibrinolysis reactions as one global assay. This study aimed to investigate the mechanisms of DVT using CFWA.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>DVT diagnosis and definition were conducted by the ultrasonography finding, and the numbers of patients of confirmed DVT and non-DVT were 39 and 56, respectively. Activated partial thromboplastin time-based CFWA was conducted, and the first-derivative curves were analyzed. The curves were separated into two phases: coagulation and fibrinolysis, and the curve shapes were compared between the two groups.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>The shapes of first-derivative curves in the coagulation phase for the DVT group were significantly different from those of the non-DVT group, and the curves of the DVT group were sharper and narrower than those of the non-DVT group. Both parameters indicating height and width in the DVT group were significantly lower than the non-DVT group. However, no significant differences were observed in the fibrinolysis phase between two groups.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>The DVT group showed different first-derivative curves in the coagulation phase compared to the non-DVT group in the CFWA assay, suggesting that the DVT group had a higher coagulability status. CFWA could be a useful tool for identifying coagulability status in patients with DVT.</p>\u0000 </section>\u0000 </div>","PeriodicalId":14120,"journal":{"name":"International Journal of Laboratory Hematology","volume":"47 3","pages":"510-519"},"PeriodicalIF":2.2,"publicationDate":"2025-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143367073","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reference Intervals and Comparative Analysis of Reticulocyte Counts Using the Mindray BC-6200, Flow Cytometry, and Manual Microscopy","authors":"Hathaiphon Aphaiwiwat,&nbsp;Chutitorn Ketloy,&nbsp;Tapakorn Thepnarin,&nbsp;Phandee Watanaboonyongcharoen,&nbsp;Eakachai Prompetchara","doi":"10.1111/ijlh.14438","DOIUrl":"10.1111/ijlh.14438","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Introduction</h3>\u0000 \u0000 <p>This study evaluated the analytical performance of the Mindray BC-6200 analyzer for reticulocyte counting, with a focus on imprecision, carryover, and time stability. The accuracy of reticulocyte count was compared with the manual microscope (MM) and flow cytometry (FC), the reference method. Additionally, reference intervals (RIs) of reticulocyte count and related parameters were established for the Thai population.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Sixty healthy Thai adults of both sexes along with 182 leftover blood samples from individuals with various pathologic conditions, were selected to evaluate reticulocyte counts using the automated Mindray BC-6200. Results were compared with MM and FC to establish RIs and assess the correlation between methods.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>The imprecision on reticulocyte counts across all control levels (coefficient of variation, %CV) was below the manufacturer's claim. Carryover was &lt; 0.001%, and time-stability was excellent up to 24 h. RIs were as follows: reticulocytes (×10⁹/L): 34.88–118.50, immature reticulocyte fraction (IRF) (%): 2.71–15.28, reticulocyte Hemoglobin content (RHE) (pg): 22.84–29.70. There was a strong correlation (<i>r</i> &gt; 0.9785) in reticulocyte counts between the automated analyzer, MM, and FC.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>The Mindray BC-6200 is a reliable alternative to MM and FC methods for reticulocyte counting, with a good correlation, precision, low carryover, and time stability, making it effective for assessing erythropoiesis in clinical settings. However, further studies are needed to evaluate the clinical utility of reticulocyte count and related parameters in diagnosing hematological conditions.</p>\u0000 </section>\u0000 </div>","PeriodicalId":14120,"journal":{"name":"International Journal of Laboratory Hematology","volume":"47 3","pages":"429-436"},"PeriodicalIF":2.2,"publicationDate":"2025-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143367075","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel Mutations in SUPT5H Gene Associated With Beta-Thalassemia Phenotype","authors":"Archana M. Agarwal,&nbsp;Anton Rets,&nbsp;Coumarane Mani","doi":"10.1111/ijlh.14431","DOIUrl":"10.1111/ijlh.14431","url":null,"abstract":"","PeriodicalId":14120,"journal":{"name":"International Journal of Laboratory Hematology","volume":"47 3","pages":"552-554"},"PeriodicalIF":2.2,"publicationDate":"2025-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143191642","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cell Population Data Parameters in Dengue—Is There Any Significance? A Single-Center Study","authors":"Jawad Hassan,&nbsp;Sarah Javed,&nbsp;Shakir Ahmed,&nbsp;Waqas Javeed,&nbsp;Madiha Abid","doi":"10.1111/ijlh.14434","DOIUrl":"10.1111/ijlh.14434","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objective</h3>\u0000 \u0000 <p>To assess the significance of cell population data parameters (CPD) in dengue positive individuals.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methodology</h3>\u0000 \u0000 <p>A retrospective cross-sectional study was conducted at the National Institute of Blood Disease and Bone Marrow Transplantation (NIBD and BMT), Karachi, Pakistan from July 2022 to September 2022 (in a period of 3 months of peak dengue fever outbreak). Dengue fever is a viral infection that is transmitted from mosquitoes to humans. It is more prevalent in tropical and subtropical environments. A total of 389 individuals, who presented with febrile illness at the NIBD clinics, were screened for dengue and malaria with Complete Blood Count (CBC), Dengue nonstructural protein 1 (NS1) antigen test and Malaria Parasite Immunochromatographic test (MP-ICT). Whole blood samples were collected and analyzed for CBC on Sysmex XN hematology analyzers. All 65 CPD and standard CBC parameters were analyzed. Statistical analysis was performed using SPSS version 25.0. Descriptive analysis of all the parameters was performed and a <i>p</i> value &lt; 0.001 was considered significant. Positive and negative correlation was also evaluated within the parameters to assess their significance. Furthermore, cut-off values of CPD parameters were evaluated plotting their receiver operating characteristic (ROC) curves.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Out of the 389 febrile patients, 137 were diagnosed as dengue-positive. Descriptive analysis for mean and median values of parameters revealed statistically significant difference for seven parameters (namely WBC, PLT-F, NEUT, LYMP, MONO, HFLC, and LY-WY) in the comparison of the two groups which were then further assessed for positive and negative correlation. Multivariate logistic regression analysis revealed High Fluorescence Lymphocyte Count (HFLC) to be the distinguishing parameter among dengue positive and negative cases. Compared to all the CPD parameters of our data set, the area under curve for lymphocytes cell size and the width of dispersion (LY-WZ) displayed a borderline value of 0.582.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>Sysmex XN hematology analyzers can provide extensive information about CPD parameters, allowing for the prompt differentiation among febrile illnesses and dengue infection. HFLC and other significant parameters demonstrate promise as rapid, adjunctive diagnostic tools. Further research is needed to validate these findings and optimize the clinical utility of CPD parameters in dengue management.</p>\u0000 </section>\u0000 </div>","PeriodicalId":14120,"journal":{"name":"International Journal of Laboratory Hematology","volume":"47 3","pages":"421-428"},"PeriodicalIF":2.2,"publicationDate":"2025-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143124297","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Whither Hematology Moving Averages?","authors":"George Cembrowski","doi":"10.1111/ijlh.14432","DOIUrl":"10.1111/ijlh.14432","url":null,"abstract":"","PeriodicalId":14120,"journal":{"name":"International Journal of Laboratory Hematology","volume":"47 3","pages":"555-556"},"PeriodicalIF":2.2,"publicationDate":"2025-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143070503","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Rare Case of Triple-Hit Ultra-High-Risk Multiple Myeloma Harboring t(4;14), t(14;20), and Gain(1q)","authors":"Jiao Lu,&nbsp;Qian Wang,&nbsp;Jing Wang,&nbsp;Zhao Zeng,&nbsp;Xiaolan Shi,&nbsp;Dandan Liu,&nbsp;Suning Chen,&nbsp;Lingzhi Yan,&nbsp;Chengcheng Fu,&nbsp;Jinlan Pan","doi":"10.1111/ijlh.14429","DOIUrl":"10.1111/ijlh.14429","url":null,"abstract":"","PeriodicalId":14120,"journal":{"name":"International Journal of Laboratory Hematology","volume":"47 3","pages":"548-551"},"PeriodicalIF":2.2,"publicationDate":"2025-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143076503","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reducing Factor V Leiden Interference in Clotting-Based Protein C Activity Assays, Including in Patients With Direct Factor Xa Oral Anticoagulant","authors":"Celia Raulet-Bussian,&nbsp;Amelie Launois,&nbsp;Fanny Perez,&nbsp;Isabelle Martin-Toutain,&nbsp;Laurie Goubeau,&nbsp;Benjamin Magnian,&nbsp;Hoai-Nam Nguyen Chi,&nbsp;Simon Chabay,&nbsp;Claire Flaujac","doi":"10.1111/ijlh.14428","DOIUrl":"10.1111/ijlh.14428","url":null,"abstract":"","PeriodicalId":14120,"journal":{"name":"International Journal of Laboratory Hematology","volume":"47 3","pages":"544-547"},"PeriodicalIF":2.2,"publicationDate":"2025-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143026106","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinicopathologic and Molecular Characterization of NUP98-Rearranged Acute Leukemias","authors":"Sujata Sajjan,&nbsp;Estelle E. Oertling,&nbsp;Franklin Fuda,&nbsp;Jeffrey Gagan,&nbsp;Prasad Koduru,&nbsp;Rolando Garcia,&nbsp;Adelaide Kwon,&nbsp;Elisa Lin,&nbsp;Miguel Cantu,&nbsp;Kathleen Wilson,&nbsp;Olga K. Weinberg,&nbsp;Mingyi Chen,&nbsp;Jesse Manuel Jaso,&nbsp;Tamra L. Slone,&nbsp;Jamie Truscott,&nbsp;Julio Alvarenga Thiebaud,&nbsp;Stephen Chung,&nbsp;Yazan F. Madanat,&nbsp;Weina Chen","doi":"10.1111/ijlh.14422","DOIUrl":"10.1111/ijlh.14422","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Introduction</h3>\u0000 \u0000 <p>\u0000 <i>NUP98</i> rearrangements are rare in acute leukemias and portend a poor prognosis.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>This study explored clinicopathologic and molecular features of five patients with <i>NUP98</i> rearranged (<i>NUP98-</i>r) acute leukemias, including three females and two males with a median age of 34 years.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>\u0000 <i>NUP98</i> fusion partners were associated with distinctive leukemia characteristics and biology. Three patients had <i>NUP98::NSD1</i>-r acute myeloid leukemia (AML, all cytogenetically cryptic and with concomitant <i>FLT3-</i>ITD) and unfavorable prognoses (in two patients), one patient had <i>NUP98::HOXA9</i>-r AML with morphologic and immunophenotypic features resembling acute promyelocytic leukemia, and lastly, one patient had previously underreported <i>NUP98::MLLT1</i>-r B/T mixed phenotype acute leukemia. After a median follow-up of 24.7 months, median overall survival was 30 months and three of five patients (60%) remained in complete remission at the last follow-up.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>Our study expands the clinical and molecular spectrum of <i>NUP98</i>-r acute leukemias and recommends FISH testing for <i>NUP98</i> rearrangement on those leukemia cases without recurrent gene rearrangements and/or normal karyotype followed by molecular confirmation to improve timely diagnosis and clinical management.</p>\u0000 </section>\u0000 </div>","PeriodicalId":14120,"journal":{"name":"International Journal of Laboratory Hematology","volume":"47 3","pages":"445-453"},"PeriodicalIF":2.2,"publicationDate":"2025-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143018913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"T-Cell Prolymphocytic Leukemia With PDGFRB Rearrangement in the Setting of Neurofibromatosis Type 1","authors":"Yue Zhao,&nbsp;Philip Petersen,&nbsp;Imran Siddiqi,&nbsp;Endi Wang","doi":"10.1111/ijlh.14427","DOIUrl":"10.1111/ijlh.14427","url":null,"abstract":"","PeriodicalId":14120,"journal":{"name":"International Journal of Laboratory Hematology","volume":"47 3","pages":"541-543"},"PeriodicalIF":2.2,"publicationDate":"2025-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143018933","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of Anticoagulants on Platelet Counts: A Study and Recommendations From the French Speaking Cellular Hematology Group (GFHC)","authors":"Soraya Wuilleme,&nbsp;Sandrine Girard,&nbsp;Michel Soulard,&nbsp;Bernard Chatelain,&nbsp;Elodie Etienne,&nbsp;Eric Guiheneuf,&nbsp;Franck Geneviève,&nbsp;Aurélie Vedrenne,&nbsp;Jean François Lesesve,&nbsp;Véronique Baccini,&nbsp;Valérie Bardet","doi":"10.1111/ijlh.14430","DOIUrl":"10.1111/ijlh.14430","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Introduction</h3>\u0000 \u0000 <p>For complete blood count, ethylenediaminetetraacetic acid (EDTA) is universally used and has been recognized as the most robust anticoagulant. However, it may lead to pseudothrombocytopenia (PTCT), due to the formation of platelet clumps, which is currently followed by resampling on sodium citrate. Other possible anticoagulants are citrate theophylline adenosine dipyridamole (CTAD) and MgSO₄. These anticoagulants were compared here for resolution of PTCT and platelet count values and stability.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Paired blood samples were used to compare the four anticoagulants. First, samples containing clumps on EDTA were compared to samples collected on sodium citrate (335), CTAD (31), or MgSO₄ (160). For platelet counts, compared series were of respectively, 168, 191, and 87, paired to PTCT-free EDTA samples. Finally, platelet count stability was evaluated over 2–24 h.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>MgSO₄, followed by CTAD, was the most efficient to avoid platelet clump formation, while sodium citrate performed poorly. Regarding platelet count, significantly lower values were obtained with sodium citrate (<i>p</i> &lt; 0.0001), with more samples (35% vs. 6.5%) below the 150 × 10⁹/L threshold. Conversely, CTAD yielded similar values as EDTA, while higher counts were observed with MgSO₄ (<i>p</i> = 0.008). Finally, platelet count stability began to decrease at 4 h for sodium citrate, while the other anticoagulants were stable for at least 8 h, up to 12 h for MgSO4 and 24 h for EDTA.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>This real-life study confirms that sodium citrate should no longer be used to improve platelet counts in patients with platelet clumps, CTAD, if available, and MgSO₄ being much better alternatives.</p>\u0000 </section>\u0000 </div>","PeriodicalId":14120,"journal":{"name":"International Journal of Laboratory Hematology","volume":"47 3","pages":"415-420"},"PeriodicalIF":2.2,"publicationDate":"2025-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143018917","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}