{"title":"Comparison of blood cell counts in leukemoid reaction and chronic myeloid leukemia: A study using Scopio blood cell counter with statistical analysis","authors":"Alaa S. Hrizat, Jerald Z. Gong","doi":"10.1111/ijlh.14341","DOIUrl":"10.1111/ijlh.14341","url":null,"abstract":"<p>Leukocytosis, defined as an increase in the number of white blood cells (WBC), is a common feature in hospitalized patients. The most common form of leukocytosis is the increase of neutrophilic granulocytes. A profound increase in neutrophilic granulocytes is also known as a leukemoid reaction (LR). Since the first introduction of the term “leukemoid reaction” by Krumbhaar in 1926,<span><sup>1</sup></span> its diagnosis has significantly improved when various laboratory methods were introduced to differentiate LR from malignant granulocytic proliferation. The prognosis for patients with LR relies primarily on their underlying causes; however, the mortality rate remains relatively high.<span><sup>2</sup></span> Therefore, a simple and reproducible method for the initial evaluation of blood, especially when ancillary studies are unavailable, is clinically paramount for improving patient care.</p><p>Different cutoffs have been reported in the literature for defining neutrophilic LR. Some authors have used a cutoff of 25.0 × 10<sup>9</sup> leukocytes/L<span><sup>3</sup></span>, while others have applied higher cutoffs of 40.0 × 10<sup>9</sup>/L, or 50.0 × 10<sup>9</sup>/L.<span><sup>4, 5</sup></span> Regardless of the cutoff, neutrophilic LR shares similar features with profound increases in neutrophils and precursors mimicking chronic leukemia of granulocytic lineage. The increase in immature forms in LR, also known as left-shift, however, is predominantly late forms (segmented neutrophils, band neutrophils) with a minority of intermediate forms (metamyelocytes, myelocytes) and no increase in early forms (promyelocytes, blasts). In addition, neutrophilic LR is usually associated with morphologic features of “toxic changes,” including heavy cytoplasmic granules (toxic granules), Dohle bodies, and cytoplasmic vacuoles. Clinical assessment of patients with potential LR relies heavily on these morphologic features.</p><p>One of the most important differential diagnoses of LR is chronic myeloid leukemia (CML), which may show similar findings when evaluating blood smears. CML is a stem cell neoplasm affecting all three lineages of hematopoietic cells, with the most profound proliferation in the granulocytic lineage. The patients typically present with marked granulocytosis with a predominance of neutrophilic granulocytes and precursors. Eosinophilic and basophilic lineages are also increased but to a lesser degree. CML can be differentiated from LR by several features when evaluating blood smears. CML shows a more prominent left-shift in neutrophilic granulocytes with the entire spectrum of immature forms. Eosinophils and basophils are also increased in CML. The granulocytic cells in CML typically lack the “toxic changes” commonly seen in LR.</p><p>The vast majority of CML and LR can be readily differentiated by a review of blood smears and clinical history. Occasionally, differentiating LR from CML can be challenging on blood smear review due to morph","PeriodicalId":14120,"journal":{"name":"International Journal of Laboratory Hematology","volume":"46 6","pages":"1125-1127"},"PeriodicalIF":2.2,"publicationDate":"2024-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/ijlh.14341","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141617796","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Steven Bruzek, Marisol Betensky, Anthony A. Sochet, Neil A. Goldenberg, Vera Ignjatovic
{"title":"Methods, precision, and analytical sensitivity of a novel low-plasma-volume assay of fibrinolytic capacity utilizing the euglobulin fraction","authors":"Steven Bruzek, Marisol Betensky, Anthony A. Sochet, Neil A. Goldenberg, Vera Ignjatovic","doi":"10.1111/ijlh.14340","DOIUrl":"10.1111/ijlh.14340","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Introduction</h3>\u0000 \u0000 <p>Fibrinolysis is a critical aspect of the hemostatic system, with assessment of fibrinolytic potential being critical to predict bleeding and clotting risk. We describe the method for a novel low-plasma-volume assay of fibrinolytic capacity utilizing the euglobulin fraction (the “modified mini-euglobulin clot lysis assay [ECLA]”), its analytic sensitivity to alterations in key fibrinolytic substrates/regulators, and its initial applications in acute and convalescent disease cohorts.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>The modified mini-ECLA requires 50 μL of plasma, a maximal read time of 3 h (with most results available within 60 min), and is entirely performed in a 96-well microplate. Assay measurements were obtained in a variety of commercial control and deficient plasmas representing clinically relevant hypo- and hyperfibrinolytic states, and in three distinct adolescent cohorts with acute or convalescent illness: critically ill, following endotracheal intubation; acute COVID-19-related illness; and ambulatory, 3 months following a venous thromboembolic event.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>In 100% and 75% deficient plasmas, hypofibrinolysis for plasminogen-deficient, fibrinolysis for alpha-2-antiplasmin-deficient, and hyperfibrinolysis for plasminogen activator inhibitor-1-deficient plasmas were observed.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>The modified mini-ECLA Clot Lysis Time Ratio (“CLTR”) demonstrated moderate-strength correlations with the Clot Formation and Lysis (CloFAL) assay, is analytically sensitive to altered fibrinolytic states in vitro, and correlates with clinical outcomes in preliminarily-studied patient populations.</p>\u0000 </section>\u0000 </div>","PeriodicalId":14120,"journal":{"name":"International Journal of Laboratory Hematology","volume":"46 6","pages":"1092-1100"},"PeriodicalIF":2.2,"publicationDate":"2024-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141565420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Didier Jambou, Noemie Saut, Viviane Queyrel, Anny Appert-Flory, Florence Fischer, Pierre Suchon, Neila De Pooter, Pierre Toulon
{"title":"F2c.*C20209T mutation in patients with a history of thrombosis: A case report, retrospective 2 site-results and review of the literature","authors":"Didier Jambou, Noemie Saut, Viviane Queyrel, Anny Appert-Flory, Florence Fischer, Pierre Suchon, Neila De Pooter, Pierre Toulon","doi":"10.1111/ijlh.14312","DOIUrl":"10.1111/ijlh.14312","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Introduction</h3>\u0000 \u0000 <p>G20210A (c.*97G>A) prothrombin gene variant, found in white population has been associated with an increased risk of venous thromboembolism (VTE). Other rare polymorphisms in F2 gene (C20209T) have been reported, more rare and touching black people, but its potential association with VTE remain uncertain.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>About a 69 years-old Caucasian woman presenting an unprovoked deep venous thrombosis of the leg, we analyzed retrospectively 25.000 thrombophilia tests on a 11-year period of time (2007–2018), at Nice and Marseille University Hospitals, and performed extensive review of the literature.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Genetic determination included a similar PCR protocol and sequencing. Twenty-one heterozygous cases out of 25.585 determinations (0.08%) was found. The C20209T mutation detected in our Caucasian patient is rare, with a frequency that differed from what was reported in the previous literature, mainly in non-Caucasian patients (Africans, Africans-Americans, and Caribbeans). One hundred and thirteen patients with this mutation have been described in the literature, of which only one homozygous.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>This study is the most important on C20209T mutation performed at present, allowing to precise its frequency and its potential role in venous thromboembolism.</p>\u0000 </section>\u0000 </div>","PeriodicalId":14120,"journal":{"name":"International Journal of Laboratory Hematology","volume":"46 5","pages":"894-898"},"PeriodicalIF":2.2,"publicationDate":"2024-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/ijlh.14312","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141556256","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Advantages of patient-based real-time quality control applications in modern quality assurance strategies","authors":"Tony Badrick, Jean-Marc Giannoli, Huub van Rossum","doi":"10.1111/ijlh.14338","DOIUrl":"10.1111/ijlh.14338","url":null,"abstract":"","PeriodicalId":14120,"journal":{"name":"International Journal of Laboratory Hematology","volume":"46 6","pages":"1123-1124"},"PeriodicalIF":2.2,"publicationDate":"2024-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141536220","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Longrong Ran, Yu Peng, Mingyu Zhao, Xin Luo, Shuang Chen, Xinyi Tang, Yakun Zhang, Lian Li, Liangmei Li, Wei Zhang, Tingting Jiang, Xuelian Wu, Renzhi Hu, Yao Liu, Zailin Yang
{"title":"Predictive model of the efficiency of hematopoietic stem cell collection in patients with multiple myeloma and lymphoma based on multiple peripheral blood markers","authors":"Longrong Ran, Yu Peng, Mingyu Zhao, Xin Luo, Shuang Chen, Xinyi Tang, Yakun Zhang, Lian Li, Liangmei Li, Wei Zhang, Tingting Jiang, Xuelian Wu, Renzhi Hu, Yao Liu, Zailin Yang","doi":"10.1111/ijlh.14337","DOIUrl":"10.1111/ijlh.14337","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Introduction</h3>\u0000 \u0000 <p>Autologous hematopoietic stem cell transplantation (ASCT) has gained extensive application in the treatment of lymphoma and multiple myeloma (MM). Plenty of studies demonstrate that peripheral blood indicators could be considered potential predictive biomarkers for hematopoietic stem cells (HSCs) collection efficiency, including white blood cell count (WBC), monocyte count (Mono), platelet count (PLT), hematocrit, and hemoglobin levels. Currently, clinically practical predictive models based on these peripheral detection indicators to quickly, conveniently, and accurately predict collection efficiency are lacking.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>In total, 139 patients with MM and lymphoma undergoing mobilization and collection of ASCT were retrospectively studied. The study endpoint was successful collection of autologous HSCs. We analyzed the effects of clinical characteristics and peripheral blood markers on collection success, and screened variables to establish a prediction model. We determined the optimal cutoff value of peripheral blood markers for predicting successful stem cell collection and the clinical value of a multi-marker prediction approach. We also established a prediction model for collection efficacy.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Univariate and multivariate logistic regression analyses showed that the mobilization regimen, Mono, PLT, mononuclear cell count (MNC), and peripheral blood CD34<sup>+</sup> cell count (PB CD34<sup>+</sup> counts) were significant predictors of successful collection of peripheral blood stem cells (PBSC). Two predictive models were constructed based on the results of multivariate logistic analyses. Model 1 included the mobilization regimen, Mono, PLT, and MNC, whereas Model 2 included the mobilization regimen, Mono, PLT, MNC, and PB CD34<sup>+</sup> counts. Receiver operating characteristic (ROC) curve analysis showed that the PB CD34<sup>+</sup> counts, Model 1, and Model 2 could predict successful HSCs collection, with cutoff values of 26.92 × 106/L, 0.548, and 0.355, respectively. Model 1 could predict successful HSCs collection with a sensitivity of 84.62%, specificity of 75.73%, and area under the curve (AUC) of 0.863. Model 2 could predict successful HSCs collection with a sensitivity of 83.52%, specificity of 94.17%, and AUC of 0.946; thus, it was superior to the PB CD34<sup>+</sup> counts alone.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>Our findings suggest that the combination of the mobilization regimen, Mono, PLT, M","PeriodicalId":14120,"journal":{"name":"International Journal of Laboratory Hematology","volume":"46 6","pages":"1068-1076"},"PeriodicalIF":2.2,"publicationDate":"2024-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/ijlh.14337","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141499998","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yolanda Posada-Franco, Ana García-Álvarez, Elena Hernández-Álvarez, Irene Serrano-García, Rocío Contera-Raposo, Mercedes Martínez-Novillo González, María Teresa Sanz-Casla
{"title":"Reference intervals for reticulocyte count and derived reticulocyte parameters in a cohort of healthy adults","authors":"Yolanda Posada-Franco, Ana García-Álvarez, Elena Hernández-Álvarez, Irene Serrano-García, Rocío Contera-Raposo, Mercedes Martínez-Novillo González, María Teresa Sanz-Casla","doi":"10.1111/ijlh.14332","DOIUrl":"10.1111/ijlh.14332","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Introduction</h3>\u0000 \u0000 <p>Reticulocyte count and novel derived parameters provide insight into the effectiveness of erythropoiesis and may be useful tools in the classification and diagnosis of anemias. However, there is no standardisation, so we consider it necessary that each laboratory evaluates the parameters according to its own methodology and instrumentation and establishes its own reference ranges. Our aim was to establish the reference intervals (RIs) of reticulocyte profile provided by the Beckman Coulter DxH 900 haematological autoanalyzer in our reference population.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>One hundred and seventy-five healthy adults (18 to 62 years) were included. Subjects were collected from the blood donation centre of the Hospital Clínico San Carlos (Madrid, Spain) upon informed consent. Whole blood was collected and assayed for 14 haematological parameters on the Beckman Coulter DxH 900 analyzer in the haematology laboratory of the Clinical Analysis Department. RIs were established as per Clinical and Laboratory Standards Institute EP28-A3c guidelines using three different statistical approaches.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>RIs estimated using the non-parametric method and the Harrell-Davis bootstrap method were very similar. RIs estimated by the robust method were narrower. Gender partitioning was required for two haematological parameters (low haemoglobin density (LHD) and microcytic anaemia factor (MAF)). The rest of the parameters did not need to be partitioned according to Lahti's method.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>RIs have been established for 14 hematologic parameters of the reticulocyte profile for the Beckman Coulter DxH 900 haematology analyzer using a healthy cohort of adult subjects.</p>\u0000 </section>\u0000 </div>","PeriodicalId":14120,"journal":{"name":"International Journal of Laboratory Hematology","volume":"46 6","pages":"997-1003"},"PeriodicalIF":2.2,"publicationDate":"2024-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141461417","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Bone marrow necrosis: Facts, controversies, and perspective","authors":"Chengxin Luan, Hongguo Zhao, Yufei Ding","doi":"10.1111/ijlh.14335","DOIUrl":"10.1111/ijlh.14335","url":null,"abstract":"<p>Bone marrow necrosis (BMN) is a clinically and pathologically poorly-defined and readily-overlooked entity. The current facts and guidelines pertaining to this entity are scarce, and there exist controversies. Upon reviewing the literature, we present the facts, analyze these controversies, and discourse on future prospects.</p>","PeriodicalId":14120,"journal":{"name":"International Journal of Laboratory Hematology","volume":"46 5","pages":"770-776"},"PeriodicalIF":2.2,"publicationDate":"2024-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/ijlh.14335","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141461327","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
V. Le Cam Duchez, C. Ternisien, E. A. Guery, V. Eschwège, E. Jeanpierre, C. Nougier, V. Proulle, A. Stepanian, M. Tuffigo, R. Marlu, C. Pouplard
{"title":"Cross-reactivity of human anti-FVIII antibodies to porcine rFVIII: French field study to validate the modified Nijmegen method","authors":"V. Le Cam Duchez, C. Ternisien, E. A. Guery, V. Eschwège, E. Jeanpierre, C. Nougier, V. Proulle, A. Stepanian, M. Tuffigo, R. Marlu, C. Pouplard","doi":"10.1111/ijlh.14331","DOIUrl":"10.1111/ijlh.14331","url":null,"abstract":"<p>Acquired hemophilia A (AHA) is a rare autoimmune bleeding disorder resulting from the development of inhibitory autoantibodies against the circulating factor VIII (FVIII:C). It occurs in patients without a family or personal history of bleeding.<span><sup>1</sup></span> AHA incidence is approximately 1.5 cases/million/year<span><sup>2</sup></span> and is idiopathic in about 50% of cases AHA.<span><sup>3</sup></span> AHA is biologically characterized by an isolated deficiency of coagulation factor VIII (FVIII:C) secondary to autoantibodies targeting specific epitopes that cause neutralization and/or accelerated clearance of FVIII from the plasma (auto-FVIII Abs).<span><sup>4</sup></span></p><p>More often, diagnosis is triggered by a bleeding event<span><sup>3</sup></span> and confirmed by laboratory data: a decreased level of FVIII:C, usually lower than 30% and the presence of anti-FVIII antibodies with a titer >0.6 Bethesda Unit/mL.<span><sup>5</sup></span> In case of severe bleeding event, an hemostatic treatment with bypassing agents, including recombinant Factor VIIa (rFVIIa) or activated prothrombin complex concentrates (aPCCs), is recommended.<span><sup>6, 7</sup></span> More recently, the recommendations for hemostatic treatment in AHA included a “new” treatment, susoctocog alfa (Obizur®): a recombinant porcine FVIII (rpFVIII).<span><sup>5</sup></span> This recombinant and highly purified protein has comparable biochemical and hemostatic properties to plasma-derived porcine factor VIII, but much lower risks of infection and toxicity. This recombinant anti-hemophilic factor porcine sequence (rpFVIII) is a B-domain deleted FVIII produced in baby hamster kidney (BHK) cells. Susoctocog alfa was approved for treatment of bleeding episodes in AHA in October 2014 in the United States and in November 2015 in Europe. Nevertheless, as recommended in the summary of product characteristics (SmPC), prior to any treatment with rpFVIII, it is necessary to test the cross-reactivity of auto-FVIII Abs with rpFVIII. A close monitoring of rpFVIII activity during treatment is also recommended.<span><sup>5</sup></span> However, some questions remain concerning this laboratory assessment. The method used for the titration of anti-porcine FVIII inhibitors is comparable to that conventionally used in our laboratories. However, SmPC notifies that the patient's plasma must be incubated with plasma titrated for porcine factor-VIII instead of the normal human plasma usually used. In addition, the reference/control should be obtained by diluting rpFVIII in plasma deficient in factor VIII and not in imidazole buffer, as usually carried out.<span><sup>8</sup></span></p><p>The aim objective of this study is first to validate in a field study the modified Nijmegen method used in our laboratories for the anti-rpFVIII titration. Ten French laboratories participated to this study and each used its local own automated, aPTT reagent and FVIII deficient plasma to perform th","PeriodicalId":14120,"journal":{"name":"International Journal of Laboratory Hematology","volume":"46 6","pages":"1118-1122"},"PeriodicalIF":2.2,"publicationDate":"2024-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/ijlh.14331","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141461415","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Zainul Islam, Mohd Adnan, Huma Noor, Rohit Chaudhary, Mohd Farhan, F. S. Sherani, Asim Rizvi
{"title":"Physiotypic variations lead to variations within the normal range for red blood cells and haemoglobin levels in a healthy human population: An evaluation using generalised additive modelling and hierarchical structure analysis","authors":"Zainul Islam, Mohd Adnan, Huma Noor, Rohit Chaudhary, Mohd Farhan, F. S. Sherani, Asim Rizvi","doi":"10.1111/ijlh.14333","DOIUrl":"10.1111/ijlh.14333","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objectives</h3>\u0000 \u0000 <p>Haematological parameters have been used for a long time for clinical evaluations, however the dynamics of these parameters has not been studied at length, in healthy populations. We aim to understand the dependence of haematological parameters on human physiotypes.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Design and Methods</h3>\u0000 \u0000 <p>Using an age and gender restricted healthy human (male) population (<i>n</i> = 100), we attempt to analyse the dynamics of haemoglobin and red blood cells, with reference to age, height and weight of individuals. Using advanced generalised additive modelling and classical hierarchical structural analysis we aim to establish relationships between these parameters and human physiotypes.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>We demonstrate that definitive relationships can be established for number of red blood cells, haemoglobin levels, RDW-CV, RDW-SD and weight, height and age of individuals.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>This study provides a proof of principle, that haematological parameters are dependent on physiotypic variation, within the normal ranges in a healthy population. It may also be noted that there is a definitive influence of height, weight and age on normal ranges and stratification by these factors might therefore make reference intervals narrower, in turn, possibly allowing more precise clinical decisions based on the complete blood count (CBC).</p>\u0000 </section>\u0000 </div>","PeriodicalId":14120,"journal":{"name":"International Journal of Laboratory Hematology","volume":"46 6","pages":"1004-1011"},"PeriodicalIF":2.2,"publicationDate":"2024-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141461416","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Mei Yang, Pinpin Liu, Xiaohong Li, Lin Zhong, Ping Qiao
{"title":"Intracellular Brucella melitensis in the cerebrospinal fluid and peripheral blood","authors":"Mei Yang, Pinpin Liu, Xiaohong Li, Lin Zhong, Ping Qiao","doi":"10.1111/ijlh.14334","DOIUrl":"https://doi.org/10.1111/ijlh.14334","url":null,"abstract":"","PeriodicalId":14120,"journal":{"name":"International Journal of Laboratory Hematology","volume":"214 1","pages":""},"PeriodicalIF":3.0,"publicationDate":"2024-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141528836","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}