International Journal of Infectious Diseases最新文献

Diagnostic accuracy of the VISITECT CD4 lateral-flow assay for screening advanced HIV disease: a systematic review and meta-analysis. VISITECT CD4侧流式检测筛查晚期HIV疾病的诊断准确性：一项系统回顾和荟萃分析

IF 4.6 2区医学

International Journal of Infectious Diseases Pub Date : 2026-09-05 DOI: 10.1016/j.ijid.2026.109101

Behailu Taye Gebremeskele, Haile Tesfaye, Workineh Woldeselassie Hammeso, Gemechu Churiso

{"title":"Diagnostic accuracy of the VISITECT CD4 lateral-flow assay for screening advanced HIV disease: a systematic review and meta-analysis.","authors":"Behailu Taye Gebremeskele, Haile Tesfaye, Workineh Woldeselassie Hammeso, Gemechu Churiso","doi":"10.1016/j.ijid.2026.109101","DOIUrl":"https://doi.org/10.1016/j.ijid.2026.109101","url":null,"abstract":"Objective: This systematic review and meta-analysis aimed to evaluate the pooled performance of the VISITECT-CD4 Advanced Disease lateral-flow assay (VISITECT-CD4-LFA) for diagnosing advanced HIV disease (AHD).Methods: A search was conducted in PubMed, EMBASE, Google Scholar, and Scopus up to February 2, 2026, to identify studies that evaluated VISITECT-CD4-LFA. Methodological quality was assessed using the Quality Assessment of Diagnostic Accuracy Studies-2 tool. Meta-analysis was conducted using a bivariate random-effects model to estimate pooled sensitivity and specificity compared to flow cytometry.Results: Ten studies were included, comprising 6962 people with HIV. Pooled sensitivity and specificity were 98% (95% CI: 94-99%) and 71% (95% CI: 56-85%), respectively, with an area under the curve (AUC) of 0.95. Subgroup analysis revealed that VISITECT-CD4-LFA from capillary blood had similar sensitivity (96%, 95% CI: 93-97%) to venous blood (99%, 95% CI: 93-100%).Conclusions: VISITECT-CD4-LFA meets the ≥90% sensitivity requirement specified in the target product profile (TPP) for point-of-care CD4 tests for AHD. With robust sensitivity using both venous and capillary blood, it has the potential to facilitate timely screening and prophylaxis for opportunistic infections in decentralized settings. The assay's pooled specificity fell slightly below the ≥80% TTP target. Further research is needed for South American and Asian populations, with strategies to boost specificity.Prospero registration: CRD420251272180.","PeriodicalId":14006,"journal":{"name":"International Journal of Infectious Diseases","volume":" ","pages":"109101"},"PeriodicalIF":4.6,"publicationDate":"2026-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148897117","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

lIs molecular rapid diagnostic test for rifampicin and isoniazid tuberculosis resistance financially feasible? An economic evaluation in Indonesia. 利福平和异烟肼结核耐药分子快速诊断试验在经济上可行吗？印度尼西亚的经济评估。

IF 4.6 2区医学

International Journal of Infectious Diseases Pub Date : 2026-09-04 DOI: 10.1016/j.ijid.2026.109099

Ibrahim Nur Insan Putra Dharmawan, Bilqis Iasha Rosma Sumroni, Salsabila Hulwani, Luthfiyah Zanida Putri, Shafira Husna, Assica Permata Amalya Hakiman, M Prasetio Wardoyo, Eghar Anugrapaksi, Budi Haryanto, Ahmad Fuady, Erlina Burhan

{"title":"lIs molecular rapid diagnostic test for rifampicin and isoniazid tuberculosis resistance financially feasible? An economic evaluation in Indonesia.","authors":"Ibrahim Nur Insan Putra Dharmawan, Bilqis Iasha Rosma Sumroni, Salsabila Hulwani, Luthfiyah Zanida Putri, Shafira Husna, Assica Permata Amalya Hakiman, M Prasetio Wardoyo, Eghar Anugrapaksi, Budi Haryanto, Ahmad Fuady, Erlina Burhan","doi":"10.1016/j.ijid.2026.109099","DOIUrl":"https://doi.org/10.1016/j.ijid.2026.109099","url":null,"abstract":"Objectives: The rifampicin and isoniazid (RIF-INH) testing provides simultaneous detection of RIF-INH resistance, yet its economic implications in Indonesia have not been evaluated. This study assessed the economic impact of implementing RIF-INH testing compared with rifampicin-only (RIF-only) testing.Methods: An economic evaluation was conducted using a decision-tree model simulating diagnostic and treatment pathways for 1,670,317 adults with presumptive pulmonary tuberculosis (TB). All TB subtypes were modeled through the first treatment cycle, capturing treatment success, treatment failure, death, and loss to follow-up (LTFU). The second cycle was limited to downstream consequences of isoniazid-resistant tuberculosis (Hr-TB) arising from the first cycle.Results: RIF-INH testing was projected to increase first-cycle direct medical costs by 8.1% while improving treatment success by 5.9%. Although first-cycle direct non-medical costs were 24.3% higher, second-cycle Hr-TB-related direct medical and non-medical costs were projected to be 57.3% and 61.0% lower, respectively, than with RIF-only testing. Probabilistic uncertainty analysis consistently showed lower downstream Hr-TB costs, whereas differences in first-cycle and total model costs remained uncertain.Conclusions: RIF-INH testing requires higher upfront costs but may reduce downstream Hr-TB-related costs. However, the overall total cost difference remains uncertain after accounting for parameter uncertainty.","PeriodicalId":14006,"journal":{"name":"International Journal of Infectious Diseases","volume":" ","pages":"109099"},"PeriodicalIF":4.6,"publicationDate":"2026-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148891688","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mixing versus matching booster vaccines: A longitudinal humoral immune kinetics against Omicron in a Malaysian cohort. 混合与匹配加强疫苗：对Omicron在马来西亚队列纵向体液免疫动力学。

IF 4.6 2区医学

International Journal of Infectious Diseases Pub Date : 2026-09-04 DOI: 10.1016/j.ijid.2026.109103

Ashwathy Varadarajan Thundakattil, Rekha Prabhu, Girish Prabhu, Muthuvel Mani, Somsubhra De, Htoo Htoo Kyaw Soe, Charlotte Patrick Arjunan, Sumandeep Kaur Chahl, Debban A L Ramesh, Ng Jian Chen, Soh Pei Qi, Jaayshree A P Prakash, Nikhytha A P Chandran, Mila Nu Nu Htay, Sabyasachi Das

{"title":"Mixing versus matching booster vaccines: A longitudinal humoral immune kinetics against Omicron in a Malaysian cohort.","authors":"Ashwathy Varadarajan Thundakattil, Rekha Prabhu, Girish Prabhu, Muthuvel Mani, Somsubhra De, Htoo Htoo Kyaw Soe, Charlotte Patrick Arjunan, Sumandeep Kaur Chahl, Debban A L Ramesh, Ng Jian Chen, Soh Pei Qi, Jaayshree A P Prakash, Nikhytha A P Chandran, Mila Nu Nu Htay, Sabyasachi Das","doi":"10.1016/j.ijid.2026.109103","DOIUrl":"https://doi.org/10.1016/j.ijid.2026.109103","url":null,"abstract":"Objective: The objective of our study was to evaluate the longitudinal humoral immune response following booster regimens in Malaysia during the Omicron outbreak.Methods: In this prospective cohort study, 268 healthcare professionals and medical students in Malaysia were followed up for 24 weeks following homologous (mRNA-mRNA (mR-mR), viral vector-viral vector (VV-VV), inactivated virus-inactivated virus (IV-IV) and heterologous (inactivated virus-mRNA (IV-mR), viral vector-mRNA (VV-mR) booster vaccines. Of them, 190 recipients underwent longitudinal immunogenicity study. Anti-S IgG and anti-RBD response were assessed by ADVIA Centaur SARS-CoV-2 chemiluminescent immunoassay followed by surrogate virus neutralization test using Omicron-variant across all boosters.Results: Compared to homologous boosters, heterologous regimens, particularly the IV-mR booster, elicited significantly higher and sustained anti-S IgG and anti-RBD antibody (P<0.0001) responses. Over 24 weeks, the heterologous IV-mR booster had 100% seropositive anti-S IgG and anti-RBD antibodies, conversely, in the homologous (IV-IV) booster, the seropositivity of anti-S IgG and anti-RBD antibody reduced to 15.63% and 18.75% respectively. Omicron-virus neutralizing activity was observed as high as 95.92% (IV-mR) and 83.87% (VV-mR) following W24 of the heterologous booster, while virus neutralization was found as low as 9.38% in the IV-IV homologous booster. Age, gender, and ethnicity were not found as confounding factors for antibody kinetics following booster vaccine.Conclusion: In conclusion, heterologous booster vaccines induced superior prolonged humoral-immune response against COVID-19 infection.","PeriodicalId":14006,"journal":{"name":"International Journal of Infectious Diseases","volume":" ","pages":"109103"},"PeriodicalIF":4.6,"publicationDate":"2026-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148890806","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Epidemiological Characteristics of Scarlet Fever in Liaoning Province, China: A Pre- and Post-Pandemic Comparison, 2005-2024. 中国辽宁省猩红热流行病学特征：2005-2024年大流行前后比较

IF 4.6 2区医学

International Journal of Infectious Diseases Pub Date : 2026-09-04 DOI: 10.1016/j.ijid.2026.109081

Meiling Jin, Hualei Xin, Jie Zhang, Xuefeng Huang, Yu Yang, Zhouchao Wang, Jianxing Yu, Lingling Mao, Wenli Diao, Yingwei Sun, Weizhong Yang, Leilei Pan, Zhongjie Li

{"title":"Epidemiological Characteristics of Scarlet Fever in Liaoning Province, China: A Pre- and Post-Pandemic Comparison, 2005-2024.","authors":"Meiling Jin, Hualei Xin, Jie Zhang, Xuefeng Huang, Yu Yang, Zhouchao Wang, Jianxing Yu, Lingling Mao, Wenli Diao, Yingwei Sun, Weizhong Yang, Leilei Pan, Zhongjie Li","doi":"10.1016/j.ijid.2026.109081","DOIUrl":"https://doi.org/10.1016/j.ijid.2026.109081","url":null,"abstract":"Objectives: This study aimed to investigate scarlet fever epidemiology in Liaoning Province, China (2005-2024), and assess COVID-19 pandemic impacts.Methods: Individual-level data on scarlet fever cases from the National Notifiable Infectious Disease Reporting System (NNIDRS) were analyzed for temporal, demographic and spatiotemporal features across pre-, peri- and post-pandemic stages.Results: A total of 73,102 scarlet fever cases were reported. Incidence remained stable during the pre-pandemic period, from 2005 (8.9 per 100,000 population per year) to 2019 (10.8), sharply declined during the pandemic period (2020-2022; range: 1.4-0.6), and rebounded markedly post-pandemic (2023: 1.9; 2024: 7.7). Most cases (79.4%) occurred in children under 15 years of age from urban areas. During 2005-2019, incidence significantly increased among children aged 3-6 years (AAPC: 7.4%; 95% CI: 3.0%-11.9%) and in rural populations (AAPC: 4.3%; 95% CI: 1.1%-7.6%). Compared with the pre-pandemic period (2005-2019), the proportion of cases among children aged 7-14 years significantly increased during the post-pandemic period (2023-2024) (31.4% vs. 54.1%, p < 0.01).Conclusion: Scarlet fever incidence in Liaoning Province showed a resurgence after the pandemic, with an age distribution shifting toward older children. These evolving epidemiological patterns highlight the need for enhanced, age-targeted surveillance and adaptive prevention strategies.","PeriodicalId":14006,"journal":{"name":"International Journal of Infectious Diseases","volume":" ","pages":"109081"},"PeriodicalIF":4.6,"publicationDate":"2026-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148891718","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Diagnosis-time Risk Stratification for Tuberculosis Mortality in a Nationwide Public-Private Mix Program. 在全国公私混合规划中结核病死亡率的诊断时间风险分层。

IF 4.6 2区医学

International Journal of Infectious Diseases Pub Date : 2026-09-04 DOI: 10.1016/j.ijid.2026.109102

Yun-Jeong Jeong, Hyung Woo Kim, Jinsoo Min, Jiwon Lyu, Jee Youn Oh, Bumhee Yang, Ganghee Chae, Goohyeon Hong, Jaehee Lee, Jonghoo Lee, Heung Bum Lee, Sung Soon Lee, Ju Sang Kim, Jae Seuk Park, Hyeon-Kyoung Koo

{"title":"Diagnosis-time Risk Stratification for Tuberculosis Mortality in a Nationwide Public-Private Mix Program.","authors":"Yun-Jeong Jeong, Hyung Woo Kim, Jinsoo Min, Jiwon Lyu, Jee Youn Oh, Bumhee Yang, Ganghee Chae, Goohyeon Hong, Jaehee Lee, Jonghoo Lee, Heung Bum Lee, Sung Soon Lee, Ju Sang Kim, Jae Seuk Park, Hyeon-Kyoung Koo","doi":"10.1016/j.ijid.2026.109102","DOIUrl":"https://doi.org/10.1016/j.ijid.2026.109102","url":null,"abstract":"Objectives: Mortality remains a major barrier to tuberculosis (TB) control, as many deaths occur shortly after diagnosis. We aimed to identify determinants of mortality and develop diagnosis-time risk-prediction models for patients with TB.Methods: We conducted a nationwide population-based cohort study using data from South Korea's public-private mix TB program, including patients diagnosed between January 2019 and December 2022. Two models were developed in the 2019-2021 cohort and validated in the independent 2022 cohort: TREAT-TB, incorporating demographic, clinical, radiologic, and microbiological variables, and SCREEN-TB, based only on demographic characteristics, symptoms, and comorbidity data. Model performance was evaluated using the area under the receiver operating characteristic curve (AUC), Brier score, and calibration analysis.Results: Among 24,745 patients, 2,667 (10.8%) died during treatment. Nearly half of the deaths occurred within two months of diagnosis. Older age, lower body mass index, and organ-specific comorbidities, particularly cardiovascular, neurological, and renal diseases, were associated with mortality. TREAT-TB and SCREEN-TB showed similar discrimination and good calibration in the validation cohort (AUCs 0.807 and 0.805, respectively), with mortality increasing across risk scores.Conclusions: Diagnosis-time risk stratification is feasible without radiologic or microbiological results and may support early triage and targeted monitoring within TB control programs.","PeriodicalId":14006,"journal":{"name":"International Journal of Infectious Diseases","volume":" ","pages":"109102"},"PeriodicalIF":4.6,"publicationDate":"2026-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148891672","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Non-invasive aspergillosis following COVID-19 exacerbates the severity of SARS-CoV-2 infection. COVID-19后的非侵入性曲霉病加重了SARS-CoV-2感染的严重程度。

IF 4.6 2区医学

International Journal of Infectious Diseases Pub Date : 2026-09-03 DOI: 10.1016/j.ijid.2026.109095

Jong Seung Kim, Jun Hyung Park, Juhyun Kim, Yong Chul Lee, Ji Won Yang, Yeon Seok You, Gwangsu Kim, Wankyu Kim, Jae Seok Jeong

{"title":"Non-invasive aspergillosis following COVID-19 exacerbates the severity of SARS-CoV-2 infection.","authors":"Jong Seung Kim, Jun Hyung Park, Juhyun Kim, Yong Chul Lee, Ji Won Yang, Yeon Seok You, Gwangsu Kim, Wankyu Kim, Jae Seok Jeong","doi":"10.1016/j.ijid.2026.109095","DOIUrl":"https://doi.org/10.1016/j.ijid.2026.109095","url":null,"abstract":"Background: Few large-scale population-based studies have examined the association between COVID-19 and respiratory aspergillosis. Using nationwide data, we investigated whether SARS-CoV-2 infection increases respiratory aspergillosis incidence and whether COVID-19-associated aspergillosis affects COVID-19 severity. We also analyzed public COVID-19 transcriptomic datasets to assess effects on airway structural and immune cells.Study design and methods: From a nationwide cohort of 8.5 million clinical registries, we included over 550,000 patients diagnosed with COVID-19 between October 8, 2020, and December 31, 2021, and matched controls. Outcomes were respiratory aspergillosis incidence, including invasive and non-invasive forms, and its association with COVID-19 severity.Results: COVID-19 was associated with increased subsequent respiratory aspergillosis. Diabetes, COPD, and other comorbidities further increased fungal infection incidence among COVID-19 patients. Both invasive and non-invasive aspergillosis were associated with greater COVID-19 severity. Systemic corticosteroid use markedly increased severity and mortality in both forms. Antifungal-related genes and pathways, including CCR6, CXCL9, and CX3CR1, were consistently downregulated after SARS-CoV-2 infection and/or corticosteroid treatment.Interpretation: COVID-19 was associated with increased respiratory aspergillosis incidence, and respiratory aspergillosis was associated with worse COVID-19 outcomes regardless of invasiveness. Further studies should evaluate whether antifungal treatment improves COVID-19 outcomes.","PeriodicalId":14006,"journal":{"name":"International Journal of Infectious Diseases","volume":" ","pages":"109095"},"PeriodicalIF":4.6,"publicationDate":"2026-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148887420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Urine-Blood Culture Concordance in Suspected Urosepsis and Implications for Early Clinical Decision-Making. 疑似尿脓毒症的尿血培养一致性及其对早期临床决策的影响。

IF 4.6 2区医学

International Journal of Infectious Diseases Pub Date : 2026-09-03 DOI: 10.1016/j.ijid.2026.109092

Philipp Grubwieser, Silke Huber, Angelika Bauer, Wolfram Mayr, Werner O Hackl, Cornelia Lass-Flörl, Werner Ruppitsch, Miriam Alisa Govrins

{"title":"Urine-Blood Culture Concordance in Suspected Urosepsis and Implications for Early Clinical Decision-Making.","authors":"Philipp Grubwieser, Silke Huber, Angelika Bauer, Wolfram Mayr, Werner O Hackl, Cornelia Lass-Flörl, Werner Ruppitsch, Miriam Alisa Govrins","doi":"10.1016/j.ijid.2026.109092","DOIUrl":"https://doi.org/10.1016/j.ijid.2026.109092","url":null,"abstract":"Objectives: To assess microbiological concordance, antimicrobial susceptibility testing (AST) agreement, and associated clinical characteristics of suspected urosepsis episodes using paired urine (UC) and blood cultures (BC).Methods: We retrospectively analyzed 2,572 paired UC-BC episodes from 2,302 adults at a tertiary-care laboratory (2018-2024). Episodes were considered concordant when UC and BC shared at least one identical pathogen. We compared pathogen spectra, culture dynamics, AST results, laboratory referral information, inflammatory markers, and survival. Multivariable logistic regression was used to identify factors associated with concordance.Results: Of 2,572 episodes, 1,405 (54.6%) were concordant. UC reports were finalized earlier than BC reports, with 50.4% available within one day. Concordant episodes showed shorter BC time-to-positivity than discordant episodes (7.8h vs 14.1h). Documented clinical suspicion and detection of Escherichia coli or Staphylococcus aureus in UC were associated with concordance. Among concordant Enterobacterales episodes, UC AST showed high agreement with BC AST. In exploratory analyses, concordant episodes were associated with faster decline of inflammatory markers and better survival.Conclusions: In suspected urosepsis, UC frequently identified the same pathogen as BC while providing earlier microbiological and susceptibility information. These findings support the potential early diagnostic and antimicrobial stewardship value of UC while BC results remain pending.","PeriodicalId":14006,"journal":{"name":"International Journal of Infectious Diseases","volume":" ","pages":"109092"},"PeriodicalIF":4.6,"publicationDate":"2026-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148887453","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rapid diagnosis of human paragonimiasis using recombinant Paragonimus westermani cysteine protease 6 antigen-based immunochromatographic devices. 重组卫氏并殖吸虫半胱氨酸蛋白酶6抗原免疫层析装置快速诊断人类并殖吸虫病。

IF 4.6 2区医学

International Journal of Infectious Diseases Pub Date : 2026-09-03 DOI: 10.1016/j.ijid.2026.109098

Patcharaporn Boonroumkaew, Lakkhana Sadaow, Nongnapas Kanchanangkul, Rutchanee Rodpai, Oranuch Sanpool, Pewpan M Intapan, Hiroshi Yamasaki, Wanchai Maleewong

{"title":"Rapid diagnosis of human paragonimiasis using recombinant Paragonimus westermani cysteine protease 6 antigen-based immunochromatographic devices.","authors":"Patcharaporn Boonroumkaew, Lakkhana Sadaow, Nongnapas Kanchanangkul, Rutchanee Rodpai, Oranuch Sanpool, Pewpan M Intapan, Hiroshi Yamasaki, Wanchai Maleewong","doi":"10.1016/j.ijid.2026.109098","DOIUrl":"https://doi.org/10.1016/j.ijid.2026.109098","url":null,"abstract":"Objectives: Cysteine proteases, the major components of excretory-secretory substances excreted from adult Paragonimus worms, are known as useful diagnostic antigens for human paragonimiasis. In this study, we develop immunochromatography-based kits to detect specific IgG and IgG4 antibodies against recombinant cysteine protease-6 (rCP-6), and to evaluate their diagnostic performance.Methods: The rCP-6 was expressed in Escherichia coli, affinity-purified, and evaluated for immunoreactivity. The rCP-6-based immunochromatographic kits were evaluated for detection of IgG and IgG4 using simulated whole-blood and serum samples from paragonimiasis patients, healthy individuals, and patients with other infectious diseases. The diagnostic performance was evaluated.Results: The IgG detection kit exhibited sensitivity of 76.7%, specificity of 90.5%, and accuracy of 87.3% for both simulated whole-blood and serum samples. In comparison, the IgG4 detection kit demonstrated higher performance, with 85.0% sensitivity, 100% specificity, and 96.5% accuracy across both sample types.Conclusions: Both IgG and IgG4 detection kits were useful for diagnosing paragonimiasis. The IgG4 detection kit showed superior diagnostic performance. These ICT tools possible be applying for diagnosing outpatients in clinical laboratories in paragonimiasis caused by different Paragonimus species (P. heterotremus, P. westermani, and P. skrjabini miyazakii).","PeriodicalId":14006,"journal":{"name":"International Journal of Infectious Diseases","volume":" ","pages":"109098"},"PeriodicalIF":4.6,"publicationDate":"2026-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148887435","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nanopore sequencing enhances the detection of low-frequency variants and mutational linkage in HIV-1 drug resistance. 纳米孔测序增强了HIV-1耐药低频变异和突变连锁的检测。

IF 4.6 2区医学

International Journal of Infectious Diseases Pub Date : 2026-09-02 DOI: 10.1016/j.ijid.2026.109093

Mengying Li, Huan Hu, Wenjie Chai, Haichao Xiao, Fei Liu, Rui Sun, Yaoguang Li, Wenhao Lyu, Hanxi Zhang, Hongxin Zhao, Fengting Yu, Fujie Zhang

{"title":"Nanopore sequencing enhances the detection of low-frequency variants and mutational linkage in HIV-1 drug resistance.","authors":"Mengying Li, Huan Hu, Wenjie Chai, Haichao Xiao, Fei Liu, Rui Sun, Yaoguang Li, Wenhao Lyu, Hanxi Zhang, Hongxin Zhao, Fengting Yu, Fujie Zhang","doi":"10.1016/j.ijid.2026.109093","DOIUrl":"https://doi.org/10.1016/j.ijid.2026.109093","url":null,"abstract":"Objectives: Low-frequency HIV-1 drug resistance mutations (DRMs) and mutation linkage within viral genomes may contribute to treatment failure and multidrug resistance but are difficult to resolve using conventional sequencing. We evaluated the performance of nanopore long-read sequencing for HIV-1 DRM detection and haplotype analysis.Methods: In this retrospective study, 230 plasma samples from people living with HIV undergoing resistance testing at Beijing Ditan Hospital between Aug 2024 and July 2025 were analysed using the nanopore-based G-seq500 platform. DRM profiles and subtype classifications were compared with Sanger sequencing, and discordant mutations were validated by next-generation sequencing (NGS, DNBSEQ-T7 platform). Long-read data were further used for haplotype reconstruction.Results: The overall concordance rate between G-seq500 and Sanger sequencing for DRM detection was 93.0%. Additional resistance-associated mutations identified by G-seq500 were confirmed by NGS and were predominantly low-frequency variants. Subtyping concordance was 92.2% (212/230). Haplotype reconstruction revealed substantial intra-host viral diversity, with nearly half of samples containing multiple viral haplotypes. Multiple DRMs frequently co-occurred within the same haplotype, indicating linked multidrug-resistant viral populations.Conclusions: G-seq500 sequencing showed comparable performance to Sanger sequencing for HIV-1 DRM detection and subtype classification. Long-read sequencing further enabled characterization of low-frequency resistance-associated variants and within-host viral haplotype structures, providing additional insights into the complexity of HIV-1 drug resistance beyond conventional consensus sequencing.","PeriodicalId":14006,"journal":{"name":"International Journal of Infectious Diseases","volume":" ","pages":"109093"},"PeriodicalIF":4.6,"publicationDate":"2026-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148880213","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rethinking pneumococcal surveillance through indoor air sampling. 重新思考通过室内空气采样进行肺炎球菌监测。

IF 4.6 2区医学

International Journal of Infectious Diseases Pub Date : 2026-09-02 DOI: 10.1016/j.ijid.2026.109091

Bieke Tack, Caspar Geenen, Tessa Nieuwenhuijsen, Lize Cuypers, Kurt Beuselinck, Krzysztof Trzciński, Emmanuel André, Stefanie Desmet

{"title":"Rethinking pneumococcal surveillance through indoor air sampling.","authors":"Bieke Tack, Caspar Geenen, Tessa Nieuwenhuijsen, Lize Cuypers, Kurt Beuselinck, Krzysztof Trzciński, Emmanuel André, Stefanie Desmet","doi":"10.1016/j.ijid.2026.109091","DOIUrl":"https://doi.org/10.1016/j.ijid.2026.109091","url":null,"abstract":"Background: Pneumococcal surveillance remains challenging due to limited invasive disease surveillance and logistically demanding nasopharyngeal carriage studies, particularly in low- and middle-income countries (LMICs). We evaluated indoor air sampling as a non-invasive approach for monitoring pneumococcal circulation and serotype distribution.Methods: Monthly indoor air samples were collected over 20 months (January 2022-September 2023) in a Belgian childcare center. Samples were analyzed by qPCR for pneumococcal detection and serotype identification.Results: Pneumococci were detected in all samples. After excluding serotypes with known specificity issues, 15 serotypes/serogroups were identified, with a mean of seven per sample. Commonly detected serotypes largely matched those reported in Belgian carriage studies and invasive disease surveillance.Conclusions: Indoor air sampling captured signals of community pneumococcal circulation and serotype dynamics. With further validation, it could provide a scalable, affordable surveillance tool to support vaccine policy, monitor vaccine impact, and strengthen respiratory pathogen surveillance, particularly in LMICs.","PeriodicalId":14006,"journal":{"name":"International Journal of Infectious Diseases","volume":" ","pages":"109091"},"PeriodicalIF":4.6,"publicationDate":"2026-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148880196","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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