International journal of clinical and experimental medicine最新文献

{"title":"Efficacy of methylprednisolone sodium succinate for injection (postotic injection) on the auditory threshold and speech recognition rate of sudden deafness patients.","authors":"Xin Li, Xiao-Yan Zhang, Qiu-Ju Wang, Da-Yong Wang","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the effect of injecting the methylprednisolone in the ear for the sudden deafness and the improvement of speech discrimination test.</p><p><strong>Methods: </strong>50 inpatients with a sudden hearing loss were recruited. Inject the methylprednisolone in the subperiosteal of the ear which is 0.5 cm distance to the ear ditch every three days. Methylprednisolone was placed for fifteen days. Simultaneously vasodilation, neurotrophic, thrombolysis and insulin hypoglycemia were administered in all patients. Pure tone test and speech discrimination test were conducted at Days 7 & 14 after intervention.</p><p><strong>Results: </strong>The outcome was as follows: cure (n = 8), efficacy (n = 9), effect (n = 18) and no effect (n = 15) respectively. The overall effective rate of 70%. The improvement of pure tone threshold and speech discrimination had significant statistical difference (P < 0.05).</p><p><strong>Conclusion: </strong>The therapy of postaural methylprednisolone injection can decrease pure tone threshold effectively and increased speech discrimination with a sudden hearing loss.</p>","PeriodicalId":13892,"journal":{"name":"International journal of clinical and experimental medicine","volume":null,"pages":null},"PeriodicalIF":0.1,"publicationDate":"2015-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4613063/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72209238","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of photosynthesis and fluorescent parameters between Dendrobium officinale and Dendrobium loddigesii.","authors":"Zhi-Rong Sun, Nan-Nan Zhu, Li-Li Cheng, Chun-Ning Yang","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the photosynthesis and fluorescent parameters between Dendrobium officinale and Dendrobium loddigesii, based on which to provide helpful information for the artificial cultivation of these cultivars.</p><p><strong>Methods: </strong>Seeds were placed on the MS medium supplemented with 0.2 mg/L NAA, 2% (w/v) sucrose, 15% (v/v) potato extracts and powered agar (pH 5.8). Two months after germination, seedlings (n = 10) were transferred onto rooting medium containing MS medium supplemented with 0.5 mg/L NAA, 3% (w/v) sucrose, 20% (v/v) potato extracts and 1‰ (w/v) activated carbon (pH 5.8) in a glass bottle (6.5 cm in diameter and 9.5 cm in height) with a white transparent plastic cap. Chlorophyll content was determined using the UV-Vis spectrophotometric method. In addition, rates of oxygen evolution and uptake were measured. The chlorophyll fluorescence was determined at room temperature using PAM 2000 chlorophyll fluorometer (Heinz Walz GmbH, Germany).</p><p><strong>Results: </strong>From month 5 to month 10, the overall contents of both chlorophyll a and chlorophyll b were higher in D. loddigesii compared with those in D. officinale. No statistical differences were observed in the apparent photosynthetic rate (APR) between D. loddigesii and D. officinale. No statistical difference was noticed in the Fo, Fm and Fv between D. loddigesii and D. officinale (P > 0.05). Significant increase was noticed in the oxygen consuming in PSI in month-8 and month-10 compared with that of month-6 in D. loddigesii. Nevertheless, in the D. officinale, the oxygen consuming in PSI in month-6 was remarkably increased with those of month-8 and month-10, respectively.</p><p><strong>Conclusions: </strong>The photosynthesis and fluorescence parameters varied in the seedling of D. loddigesii and D. officinale. Such information could contribute to the artificial cultivation of these cultivars.</p>","PeriodicalId":13892,"journal":{"name":"International journal of clinical and experimental medicine","volume":null,"pages":null},"PeriodicalIF":0.1,"publicationDate":"2015-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4612924/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140193704","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Expression of Toll-like receptor 4 in ovarian serous adenocarcinoma and correlation with clinical stage and pathological grade.","authors":"Xue-Zhen Luo,&nbsp;Qi-Zhi He,&nbsp;Kai Wang","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Toll-like receptor 4 (TLR4) plays an essential role in adaptive and innate immunity, and its expression has been described in various tumors. This study aimed to examine the expression of TLR4 in serous tumors and to evaluate its correlation to clinicopathological parameters. The expression of TLR4 was immunohistochemically examined in 63 species of normal ovarian epithelia and 336 species of serous epithelial lesions. Moreover, the association between TLR4 expression and various clinicopathologic features was assessed. The expression intensity of TLR4 in benign and borderline to malignant ovarian tumours showed a gradual rising trend. We identified positive correlations between TLR4 expression levels and both FIGO stage and pathological stage. In serous adenocarcinoma, TLR4 expression levels were significantly associated with chemoresistance. There was no relationship between the expression of TLR4 and the patient's age or pretreatment serum CA125 levels. Our data suggest that TLR4 might stimulate serous ovarian carcinoma initiation and progression. TLR4 expression is correlated with poor chemoresponse, which has important implications for the development of new therapeutic strategies for drug-resistant ovarian cancer. </p>","PeriodicalId":13892,"journal":{"name":"International journal of clinical and experimental medicine","volume":null,"pages":null},"PeriodicalIF":0.1,"publicationDate":"2015-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4613102/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71434229","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Infective endocarditis: a tertiary referral centre experience from Turkey.","authors":"Fahriye Vatansever Agca, Necmiye Demircan, Tezcan Peker, Hasan Ari, Kemal Karaagac, Ozlem Arican Ozluk, Mustafa Yilmaz, Erhan Tenekecioglu","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Introduction: </strong>We aimed to define the current characteristics of infective endocarditis (IE) in a part of Turkey.</p><p><strong>Methods: </strong>All patients who were hospitalized in our hospital with a diagnosis of IE between 2009 and 2014 were included in the study. Data were collected from archives records of all patients. Modified Duke criteria were used for diagnosis.</p><p><strong>Results: </strong>There were 85 IE cases during the study period. The mean age of patients was 52 years. Fourty eight of patients were males. Native valves involved in 47%, prostetic valves involved in 40% and pacemaker or ICD lead IE in 13% of patients. Mitral valve was the most common site of vegetationb (38%). The most common valvular pathology was mitral regurgitation. The most common predisposing factor was prosthetic valve disease (40%). Positive culture rate was 68%. Staphylococci were the most frequent causative microorganisms isolated (27%) followed by Streptococcus spp. (11%). In-hospital mortality rate was 36%.</p><p><strong>Conclusion: </strong>In Turkey, IE occurs in relatively young patients. In high developed part of Turkey, prosthetic and dejenerative valve disease is taking the place of rheumatic valve disease as a predisposing factor. Surgery is an important factor for preventing mortality.</p>","PeriodicalId":13892,"journal":{"name":"International journal of clinical and experimental medicine","volume":null,"pages":null},"PeriodicalIF":0.1,"publicationDate":"2015-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4613038/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140195440","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Application of MSCTA combined with VRT in the operation of cervical dumbbell tumors.","authors":"Wan Wang, Jia Lin, Engelbert Knosp, Yuanzheng Zhao, Dianhui Xiu, Yongchuan Guo","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Cervical dumbbell tumor poses great difficulties for neurosurgical treatment and incurs remarkable local recurrence rate as the formidable problem for neurosurgery. However, as the routine preoperative evaluation scheme, MRI and CT failed to reveal the mutual three-dimensional relationships between tumor and adjacent structures. Here, we report the clinical application of MSCTA and VRT in three-dimensional reconstruction of cervical dumbbell tumors. From January 2012 to July 2014, 24 patients diagnosed with cervical dumbbell tumor were retrospectively analyzed. All patients enrolled were indicated for preoperative MSCTA/VRT image reconstruction to explore the three-dimensional stereoscopic anatomical relationships among neuroma, spinal cord and vertebral artery to achieve optimal surgical approach from multiple configurations and surgical practice. Three-dimensional mutual anatomical relationships among tumor, adjacent vessels and vertebrae were vividly reconstructed by MSCTA/VRT in all patients in accordance with intraoperative findings. Multiple configurations for optimal surgical approach contribute to total resection of tumor, minimal damage to vessels and nerves, and maximal maintenance of cervical spine stability. Preoperative MSCTA/VRT contributes to reconstruction of three-dimensional stereoscopic anatomical relationships between cervical dumbbell tumor and adjacent structures for optimal surgical approach by multiple configurations and reduction of intraoperative damages and postoperative complications. </p>","PeriodicalId":13892,"journal":{"name":"International journal of clinical and experimental medicine","volume":null,"pages":null},"PeriodicalIF":0.1,"publicationDate":"2015-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4613070/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141064293","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Retaining of PTCA guide wire in the left ventricular lead and subsequent application of epicardial electrode when CRT-D implantation in a patient with severe heart failure and persistent left superior vena cava: a case report.","authors":"Pei-Pei Hou, Yu-Hong Liu, Hai-Bo Qu, Jin Meng, Qiang Li, Zhi-Lin Miao","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>One patient with severe heart failure (LV 92 mm, EF 28%) was treated by cardiac resynchronization therapy (CRT).</p><p><strong>Method: </strong>During the operation, it was found that double superior vena cava coexisted, and selective coronary venography cannot clearly show every branch. It was difficult to push ventriculus sinister electrode to sideward vein, so the electrode was released to far end of frontal septal branch along great cardiac vein.</p><p><strong>Result: </strong>However, because of insufficient braced force of ventriculus sinister electrode, 0.014 PTCA guide wire was detained in the electrode. 2 years later, two spots of PTCA guide wire retained in ventriculus sinister electrode broke in atrium dextrum, so the implantation of epicardial electrode was conducted.</p><p><strong>Conclusion: </strong>After the operation, heart failure was relieved. After 43 months, the battery of pacemaker depleted, so the pacemaker was changed. The effect since follow-up visit was good, LV decreased to 86 mm, EF increased to 32%, and SPWMD time limit shortened from 147 ms to 45 ms. The therapeutic experience of this patient indicated that the effect of detaining PTCA guide wire to enhance braced force in implantation of ventriculus sinister is unreliable and inappropriate to be advocated.</p>","PeriodicalId":13892,"journal":{"name":"International journal of clinical and experimental medicine","volume":null,"pages":null},"PeriodicalIF":0.1,"publicationDate":"2015-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4613122/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141064427","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Predictive accuracy comparison of MELD and Child-Turcotte-Pugh scores for survival in patients underwent TIPS placement: a systematic meta-analytic review.","authors":"Chunze Zhou, Changlong Hou, Delei Cheng, Wenjing Tang, Weifu Lv","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Background: </strong>Child-Turcotte-Pugh (CTP) and the model for end-stage liver disease (MELD) scores have been used commonly to predict the survival in the patients with liver diseases underwent transjugular intrahepatic portosystemic shunt (TIPS). However, a debate has continued for years whether CTP could be replaced by MELD score. We performed a systematic meta-analytic review to compare the prediction capability of both scores in survival among patients with TIPS.</p><p><strong>Methods: </strong>Retrospective cohort studies among patients with TIPS were published as of May 2013 were identified by systematically searching four electronic literature database, such as Ovid Medline, PubMed, EMBASE, and ISI Web of Science. The difference of standardized mean difference (SMD) of c-statistics for the predictive accuracy of 1-, 3-, 6-, and 12-month survival for both MELD and CP scores, defined as effect size (ES), was calculated for each individual study and then pooled across studies using standard meta-analyses with a random effects model. Publication bias was evaluated using funnel plots and Kendall's rank correlation tests.</p><p><strong>Results: </strong>174 researches articles or conference abstracts were searched and reviewed using the combination of relevant terms in the articles. Finally, 11 articles were defined as eligible studies to evaluate simultaneously the predictive accuracy of MELD and CTP scores. In the meta-analyses, MELD score was superior to CP score in predicting 3-month survival after TIPS (mean ES, 0.63; 95% confidence interval [CI], 0.13-1.14; P=0.01), but the predictive capability in 1-month, 6-month, and 12-month survival was not significant (1-month: mean ES, 0.79; 95% CI, -0.24-1.83; P=0.13; 6-month: mean ES, 0.46; 95% CI, -2.46-3.37; P=0.76; 12-month: mean ES, 0.36; 95% CI, -0.25-0.96; P=0.25).</p><p><strong>Conclusions: </strong>No enough evidence are confirmed so far that MELD score is better than CTP score to assess the overall prognosis after TIPS, especially long-term predictions, but 3-month predictive capability of MELD score significantly outperform CTP score.</p>","PeriodicalId":13892,"journal":{"name":"International journal of clinical and experimental medicine","volume":null,"pages":null},"PeriodicalIF":0.1,"publicationDate":"2015-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4612968/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141064416","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association between clusterin polymorphisms and esophageal squamous cell carcinoma risk in Han Chinese population.","authors":"Kun Li, Jian Wang, Zhen-Bin Ma, Guang-Hong Guo","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Genetic susceptibility plays an essential role in an individual's risk of esophageal squamous cell carcinoma (ESCC). The aim of this study is to investigate the associations between clusterin (CLU) gene polymorphisms and ESCC risk. We undertook a case-control study to analyze three CLU polymorphisms (gene rs9331888 C>G, rs17466684 A>G and rs1532278 T>C) in an Han Chinese population, by extraction of genomic DNA from the peripheral blood of 642 patients with ESCC and 658 control participants, and performed CLU genotyping using DNA sequencing. The obtained results indicated that overall, no statistically significant association was observed in rs17466684 and rs1532278. However, gene rs9331888 C>G genotype was at increased risk of ESCCs (P=0.037; odds ratio (OR)=1.089, 95% CI: 1.006-1.175). Moreover, rs9331888 G/G genotype ESCCs were more significantly common in patients with tumor size of >5 cm than T allele ESCC and in cases of poor differentiation and lower advanced pathological stage. In conclusion, polymorphism in rs9331888 C>G was observed to be associated with susceptibility of ESCC. Nevertheless, further investigation with a larger sample size is needed to support our results. </p>","PeriodicalId":13892,"journal":{"name":"International journal of clinical and experimental medicine","volume":null,"pages":null},"PeriodicalIF":0.1,"publicationDate":"2015-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4613044/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140193703","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reduction of α1GABAA receptor mediated by tyrosine kinase C (PKC) phosphorylation in a mouse model of fragile X syndrome.","authors":"Weidong Zhao, Jiaqin Wang, Shunyi Song, Fang Li, Fangfang Yuan","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Fragile X syndrome (FXS) caused by lack of fragile X mental retardation protein (Fmr1) is the most common cause of inherited intellectual disability and characterized by many cognitive disturbances like attention deficit, autistic behavior, and audiogenic seizure and have region-specific altered expression of some gamma-aminobutyric acid (GABAA) receptor subunits. Quantitative real-time polymerase chain reaction and western blot experiments were performed in the cultured cortical neurons and forebrain obtained from wild-type (WT) and Fmr1 KO mice demonstrate the reduction in the expression of α1 gamma-aminobutyric acid (α1GABAA) receptor, phospho-α1GABAA receptor, PKC and phosphor-PKC in Fmr1 KO mice comparing with WT mice, both in vivo and in vitro. Furthermore, we found that the phosphorylation of the α1GABAA receptor was mediated by PKC. Our results elucidate that the lower phosphorylation of the α1GABAA receptor mediated by PKC neutralizes the seizure-promoting effects in Fmr1 KO mice and point to the potential therapeutic targets of α1GABAA agonists for the treatment of fragile X syndrome. </p>","PeriodicalId":13892,"journal":{"name":"International journal of clinical and experimental medicine","volume":null,"pages":null},"PeriodicalIF":0.1,"publicationDate":"2015-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4612931/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140193736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigation of relationship of visceral body fat and inflammatory markers with metabolic syndrome and its components among apparently healthy individuals.","authors":"Yasemin Turker, Davut Baltaci, Yasin Turker, Serkan Ozturk, Cemil Isik Sonmez, Mehmet Harun Deler, Yunus Cem Sariguzel, Feyza Sariguzel, Handan Ankarali","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Metabolic syndrome is a cluster of disorders and great risk for cardiovascular diseases. We aimed to investigate association between severity of metabolic syndrome (MetS) and anthropometric measurements, and to evaluate correlation of MetS and its components with metabolic deterioration and inflammatory indexes. The cross-sectional study enrolled 1474 patients with obesity and overweight. The patients were grouped as MetS and Non-MetS, and were sub-grouped as group 1 (three criteria), 2 (four criteria) and 3 (≥ five criteria) according to NCEP ATP III. Mean age was 38.7 ± 11.9 years and BMI was 35.1 ± 6.3 kg/m(2). Lipid profile, anthropometric and blood pressure measurements, liver function tests, bioelectric impedance body fat compositions, insulin resistance and HbA1c, and spot urinary albumin-creatinine ratio were significantly different between groups of MetS and Non-MetS. Age, lipid profile, bioelectric impedance fat analyses, BMI, blood pressure values, glucose, insulin resistance, uric acid and hs-CRP levels were significantly different between groups of MetS component groups. ROC analysis revealed that hs-CRP was found to be more predictive for severity of metabolic syndrome components 3 and 4 (P=0.030); uric acid and visceral fat were more actual to predict severity of metabolic syndrome between 3 and 5 MetS components, (P=0.006) and uric acid was detected as more actual to predict severity of MetS between 4 and 5 components (P=0.023). In conclusion, uric acid, hs-CRP and visceral body fat composition were useful to predict to severity of MetS in primary care. </p>","PeriodicalId":13892,"journal":{"name":"International journal of clinical and experimental medicine","volume":null,"pages":null},"PeriodicalIF":0.1,"publicationDate":"2015-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4612914/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140193734","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}