International journal of clinical and experimental medicine最新文献

{"title":"Ginkgetin attenuates metastasis of breast cancer via inhibition of the Hedgehog signaling pathway","authors":"Wen-Na Yang","doi":"10.62347/abao1134","DOIUrl":"https://doi.org/10.62347/abao1134","url":null,"abstract":"e-Century Publishing Corporation, Madison, Wisconsin, USA publishes 17 medical journals, with 13 of them officially indexed in PubMed and archived in PubMed Central, 11 of them are indexed in SCI Web of Science (2 in SCIE with Impact Factors and 8 in ESCI.","PeriodicalId":13892,"journal":{"name":"International journal of clinical and experimental medicine","volume":"18 4","pages":"101-111"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147891696","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ginkgolic acids (C15 and C17) induce autophagy and apoptosis in gastric carcinoma HGC cells by inducing ER stress","authors":"Mu‐Bing Wang","doi":"10.62347/jjvs1398","DOIUrl":"https://doi.org/10.62347/jjvs1398","url":null,"abstract":"Objective: This paper show that Ginkgolic acids can induce cell death in gastric cancer cell line HGC via a combination of autophagy and apoptosis pathway.Methods: The survival curves of the GAs (C15, C17) of the HGC were measured by MTT assay, and the expression of apoptosis associated proteins caspase-3, 8, 9 and the autophagy-associated protein 5, beclin 1 and microtubule-associated protein 1A/1B-light chain 3 in the GA-treated HGC cells were all measured by western blot analysis.The terminal deoxynucleotidyl-transferase-mediated dUTP nick-end labeling (TUNEL) assay was used to monitor the level of apoptosis in the GA-treated cells, and Transmission Electron Microscopy (TEM) was used to monitor the level of autophagy in the GA-treated cells.Results: MTT assays showed that both C15 and C17 inhibited HGC cell proliferation in a time-dependent and dose-dependent manner, and WB showed that the ER stress-, autophagy-, and apoptosis-related proteins were all increased.TUNEL, and TEM analysis show that the GAs induced both apoptosis and autophagy in the HGC cells.After CHOP expression was knocked down by small interfering RNA (siRNA), both C15 and C17 ginkgolic acid-treated cells showed increased ER stress-related protein expression, moreover, the LC3-II conversion and beclin-1 expression were reduced.After inhibition of ER stress by 4-PBA, both autophagy-related protein and apoptosis-related protein expression was decreased.After treatment with the autophagy inhibitor 3MA, both ER stress-related protein and apoptosis-related protein expression was decreased.After treatment with the c-Jun N-terminal kinase (JNK) pathway downregulator SP600125, the expression of beclin-1 and LC3-II conversion were reduced.Conclusions: The results suggest that both C15 and C17 ginkgolic acids increased ER stress upstream to regulate autophagy and apoptosis by three branch pathways (ATF6, inositol-requiring enzyme 1 (IRE1), and PKR-like ER protein kinase (PERK)).The IRE1-JNK pathway plays an important role in the crosstalk between ER stress and autophagy.","PeriodicalId":13892,"journal":{"name":"International journal of clinical and experimental medicine","volume":"18 2","pages":"18-29"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://doi.org/10.62347/jjvs1398","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147877861","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isosinensetin attenuates the growth of colorectal cancer cells by targeting the USP10/c-Myc signaling pathway","authors":"Yu‐Chi Wu","doi":"10.62347/ssif8218","DOIUrl":"https://doi.org/10.62347/ssif8218","url":null,"abstract":"e-Century Publishing Corporation, Madison, Wisconsin, USA publishes 17 medical journals, with 13 of them officially indexed in PubMed and archived in PubMed Central, 11 of them are indexed in SCI Web of Science (2 in SCIE with Impact Factors and 8 in ESCI.","PeriodicalId":13892,"journal":{"name":"International journal of clinical and experimental medicine","volume":"18 8","pages":"208-219"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147889959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Overexpression of DOCK6 correlates with poor survival of patients with epithelial ovarian cancer","authors":"Ping Wang","doi":"10.62347/oxru9072","DOIUrl":"https://doi.org/10.62347/oxru9072","url":null,"abstract":"","PeriodicalId":13892,"journal":{"name":"International journal of clinical and experimental medicine","volume":"17 11","pages":"198-205"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://doi.org/10.62347/oxru9072","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147922446","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MiR-203a inhibits gastric stem cancer cells by targeting mTOR","authors":"Ting Liu","doi":"10.62347/zwca4205","DOIUrl":"https://doi.org/10.62347/zwca4205","url":null,"abstract":"To evaluate the role and underlying mechanism of microRNA (miR)-203a in gastric stem cancer cells.Reverse transcription quantitative polymerase chain reaction was used to determine the expression of miR-203a in the adjacent cancerous and non-cancerous tissues.Cell proliferation and cellular drug resistance was detected using Cell Counting Kit-8 and EDU assays, and cell migration and invasion were detected using transwell assays.RNA sequencing and database website predictions confirmed the downstream target genes, which was further confirmed by double-luciferase reporter gene experiments.Attenuated miR-203a expression was observed in gastric cancer, and its expression level was associated with tumor size, tumor node metastasis stage, tissue differentiation, and patient survival.Overexpression of miR-203a in gastric stem cancer cells significantly inhibited cell proliferation, migration, invasion and cellular drug resistance, whereas silencing its expression resulted in the opposite effects.Double-luciferase reporter gene experiments confirmed that miR-203a acts directly on the downstream target gene, mTOR.Cellular mTOR upregulation restores the inhibitory effect of miR-203a overexpression on gastric stem cancer cell proliferation, migration, invasion and drug resistance.MiR-203a inhibits the malignant behavior of gastric stem cancer cells by targeting mTOR.","PeriodicalId":13892,"journal":{"name":"International journal of clinical and experimental medicine","volume":"17 2","pages":"8-18"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://doi.org/10.62347/zwca4205","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147896786","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A case report of moderate COVID-19 with an extremely long-term viral shedding period in China","authors":"Yonghong Wang, Chao-Nan Liu, Qinghui Meng, Shuang Gui, Yu Wu, P. Cheng, Peng Wang, Xiu-Yun Liao","doi":"10.21203/rs.3.rs-23009/v1","DOIUrl":"https://doi.org/10.21203/rs.3.rs-23009/v1","url":null,"abstract":"\u0000 Background: An ongoing outbreak of novel coronavirus disease 2019 (COVID-19) from Wuhan, China, is currently recognized as a global public health emergency, which has subsequently spread to the rest of China and other countries. The WHO raised the COVID-19 alert to the highest level. The virus is a new highly contagious via human-to-human transmission. The median duration of viral shedding is 20.0 days. We report that the longest duration of viral shedding was 32.0 days from illness onset in a patient with moderate COVID-19 admitted to QianJiang Central Hospital.Case presentation: A 37-year-old male sought medical advice while suffering from fever, dry cough, fatigue, dizziness, runny nose and diarrhoea. Five days before the visit, he had a history of travel from affected geographic areas. The patient had a positive RT-PCR test, and chest CT images showed multiple nodules and mixed ground-glass opacification with consolidation in both lungs. Laboratory findings showed that his lymphocyte and CD4+ counts were below the normal range. The patient was given antiviral treatment, including arbidol, lopinavir, IFN-α, and traditional Chinese medicine, and other necessary support care. All clinical symptoms and CT imaging manifestation abnormalities resolved during the course of therapy.Conclusion: Although the positive RT-PCR tests were verified in consecutive upper respiratory specimens, the clinical symptoms, CT imaging findings, CD4+ lymphocyte counts, and IgG antibody levels had obviously improved. Positive tests may be detecting pieces of inactive viruses, which would not be transmissible in individual cases.","PeriodicalId":13892,"journal":{"name":"International journal of clinical and experimental medicine","volume":"15 1","pages":""},"PeriodicalIF":0.1,"publicationDate":"2020-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43533437","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lentivirus-Mediated knockdown of tectonic family member 1 inhibits medulloblastoma cell proliferation [Retraction].","authors":"","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>[This retracts the article on p. 13127 in vol. 8, PMID: 26550235.].</p>","PeriodicalId":13892,"journal":{"name":"International journal of clinical and experimental medicine","volume":"11 3","pages":"2917"},"PeriodicalIF":0.1,"publicationDate":"2018-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5892459/pdf/ijcem0011-2917.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36198357","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Classification of MRI and psychological testing data based on support vector machine.","authors":"Wenlu Yang, Xinyun Chen, David S Cohen, Eric R Rosin, Arthur W Toga, Paul M Thompson, Xudong Huang","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Alzheimer's disease (AD) is a progressive, and often fatal, brain disease that causes neurodegeneration, resulting in memory loss as well as other cognitive and behavioral problems. Here, we propose a novel multimodal method combining independent components from MRI measures and clinical assessments to distinguish Alzheimer's patients or mild cognitive impairment (MCI) subjects from healthy elderly controls. 70 AD subjects (mean age: 77.15 ± 6.2 years), 98 MCI subjects (mean age: 76.91 ± 5.7 years), and 150 HC subjects (mean age: 75.69 ± 3.8 years) were analyzed. Our method includes the following steps: pre-processing, estimating the number of independent components from the MR image data, extracting effective voxels for classification, and classification using a support vector machine (SVM)-based classifier. As a result, with regards to classifying AD from healthy controls, we achieved a classification accuracy of 97.7%, sensitivity of 99.2%, and specificity of 96.7%; for differentiating MCI from healthy controls, we achieved a classification accuracy of 87.8%, a sensitivity of 86.0%, and a specificity of 89.6; these results are better than those obtained with clinical measurements alone (accuracy of 79.5%, sensitivity of 74.0%, and specificity of 85.1%). We found that (1) both AD patients and MCI subjects showed brain tissue loss, but the volumes of gray matter loss in MCI subjects was far less, supporting the notion that MCI is a prodromal stage of AD; and (2) combining gray matter features from MRI and three commonly used measures of mental status, cognitive function improved classification accuracy, sensitivity, and specificity compared with classification using only independent components or clinical measurements.</p>","PeriodicalId":13892,"journal":{"name":"International journal of clinical and experimental medicine","volume":"10 12","pages":"16004-16026"},"PeriodicalIF":0.1,"publicationDate":"2017-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5808983/pdf/nihms939321.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35832873","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of lipiodol and methylene blue on the thoracoscopic preoperative positioning [Retraction].","authors":"","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>[This retracts the article on p. 7569 in vol. 8, PMID: 26221301.].</p>","PeriodicalId":13892,"journal":{"name":"International journal of clinical and experimental medicine","volume":"10 11","pages":"15801"},"PeriodicalIF":0.1,"publicationDate":"2017-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5724625/pdf/ijcem0010-15801.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36198355","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nucleostemin regulates proliferation and migration of gastric cancer and correlates with its malignancy [Retraction].","authors":"","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>[This retracts the article on p. 17634 in vol. 8, PMID: 26770353.].</p>","PeriodicalId":13892,"journal":{"name":"International journal of clinical and experimental medicine","volume":"10 11","pages":"15800"},"PeriodicalIF":0.1,"publicationDate":"2017-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5724623/pdf/ijcem0010-15800.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35766498","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}