International journal of clinical and experimental medicine最新文献

{"title":"Postmortem redistribution of lidocaine after epidural injection in beagle dogs.","authors":"Chao Zhang, Bin Liang, Chendan Wang, Zhiwen Wei, Keming Yun","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objectives: </strong>An animal model using beagle dog has been established to investigate the postmortem redistribution of lidocaine.</p><p><strong>Materials and methods: </strong>18 dogs were euthanized and injected lidocaine (13 mg/kg) via epidural immediately. An autopsy was performed at 0, 12, 24, 48, 72, 96 hours after drug administration. All animals were stored in supine position at room temperature. For the other groups, lidocaine was given via epidural 6, 12, 24 hours after dogs were euthanized. Followed treatments were as above described. All samples were treated for detection of the concentration of lidocaine.</p><p><strong>Results: </strong>It was found that lidocaine could diffuse via blood vessel rapidly post administration. And the concentration of lidocaine in the blood from ventriculus sinister increased obviously in a time-dependent manner. Meanwhile, the postmortem tissue distribution of lidocaine was significantly different. However, the process of postmortem redistribution of lidocaine was obviously delayed in dogs which were given drugs after death.</p><p><strong>Conclusions: </strong>Together results revealed the process of postmortem redistribution of lidocaine via epidural injection, and provided the method to distinguish the lidocaine-induced death and drug administration after death.</p>","PeriodicalId":13892,"journal":{"name":"International journal of clinical and experimental medicine","volume":"8 10","pages":"18500-5"},"PeriodicalIF":0.1,"publicationDate":"2015-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4694359/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141081269","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficiency of muscle strength training on motor function in patients with coronary artery disease: a meta-analysis.","authors":"Yu-Jie Yang, Xiao-Hua He, Hai-Ying Guo, Xue-Qiang Wang, Yi Zhu","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Background: </strong>Existing literature has shown that patients with coronary artery disease (CAD) can benefit greatly from the strength training; therefore, the strength training should play a more important role in cardiac rehabilitation. However, the medical community may still have conservation to apply the strength training owing to no comprehensive study so far to compare the effectiveness of the strength training to the other trainings, such as aerobic training.</p><p><strong>Objective: </strong>To evaluate the effect of strength training on motor function in patients with CAD.</p><p><strong>Methods: </strong>Published articles from the earliest date available to July 2015 were identified using electronic searches. Two reviewers selected independently relevant randomized controlled trials (RCTs) investigating exercise program with strength training versus control interventions (exercise without strength training, including aerobic training and no exercise group) for the treatment of CAD patients. We examined effects of exercise with strength training versus control interventions on peak oxygen uptake (VO2peak), duration of exercise test and muscle strength. Two reviewers extracted data independently.</p><p><strong>Results: </strong>Twenty seven trials that represented 1151 participants passed the selection criteria and were evaluated for the effects of strength training in CAD patients. For improving VO2peak [SMD (95%CI) = 0.58 (0.11, 1.06)] and muscle strength [upper limb, SMD (95% CI) =0.44 (0.34, 0.55); lower limb, SMD (95% CI) =0.33 (0.16, 0.50)], exercise program with strength training were significantly more effective than one without it. But there is no significantly difference on duration of exercise test [SMD (95%CI) = 0.17 (-0.04, 0.39)] in strength training group than in control group.</p><p><strong>Conclusions: </strong>We conclude strength training is effective in improving muscle strength and VO2peak, in CAD patients, when compared to patients with control group. Furthermore, our evaluations suggest that strength training does not compromise clinical trial completion or safety.</p>","PeriodicalId":13892,"journal":{"name":"International journal of clinical and experimental medicine","volume":"8 10","pages":"17536-50"},"PeriodicalIF":0.1,"publicationDate":"2015-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4694245/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141081242","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Age plays an important role in the relationship between smoking status and obesity risk: a large scale cross-sectional study of Chinese adults.","authors":"Pu Su, Liu Hong, Hang Sun, Yi Fan Zhao, Liang Li","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>To study the role of age plays in the relationship between smoking status and obesity in both Chinese men and women.</p><p><strong>Methods: </strong>From Chinese Physical and Psychological Database, participants were divided into non-smokers, current smokers, and former smokers. Body mass index (BMI), waist circumference (WC), fat percentage, fat mass, and fat free mass were measured. The mean, standard deviation and frequency of these indicators were calculated for each age bracket. One-way ANOVA and post-hoc test analyses were used to detect the difference among these three groups.</p><p><strong>Results: </strong>In men, from 19 to 24 years old, BMI, WC and fat free mass of current smokers were higher than that of non-smokers (P<0.01). However, fat mass and fat percentage of current smokers were lower than that of non-smokers but higher than that of former smokers (P<0.01). From 25 to 34 years old, BMI and fat mass of former smokers were higher than non-smokers and current smokers (P<0.01). In addition, WC and fat free mass of non-smokers were lower than that of current smokers and former smokers (P<0.01). From 45 to older, BMI, WC, fat mass, fat free mass and fat percentage of former smokers were higher than that of current smokers (P<0.01). From 55 to older, BMI, WC, fat mass, fat free mass and fat percentage of current smokers were lower than that of non-smokers (P<0.01). In women, smoking status might not be significantly related to obesity (P>0.05).</p><p><strong>Conclusion: </strong>For young men, smoking might have an effect on increasing fat free mass, BMI and WC, and decreasing fat mass and fat percentage. For middle and older men, smoking might have an effect on decreasing fat free mass, fat mass, BMI, WC, and fat percentage. Obesity risk should be paid more attention in smoking cessation programs for those former smokers.</p>","PeriodicalId":13892,"journal":{"name":"International journal of clinical and experimental medicine","volume":"8 10","pages":"18894-906"},"PeriodicalIF":0.1,"publicationDate":"2015-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4694414/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140193697","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of cell printing on biological characters of chondrocytes.","authors":"M. Qu, Xiaoyan. Gao, Yikang Hou, Congcong Shen, Yourong Xu, Ming-sheng Zhu, Hengjian Wang, Haisong Xu, G. Chai, Yan Zhang","doi":"10.1166/JBT.2016.1420","DOIUrl":"https://doi.org/10.1166/JBT.2016.1420","url":null,"abstract":"OBJECTIVE\u0000To establish a two-dimensional biological printing technique of chondrocytes and compare the difference of related biological characters between printed chondrocytes and unprinted cells so as to control the cell transfer process and keep cell viability after printing.\u0000\u0000\u0000METHODS\u0000Primary chondrocytes were obtained from human mature and fetal cartilage tissues and then were regularly sub-cultured to harvest cells at passage 2 (P2), which were adjusted to the single cell suspension at a density of 1×10(6)/mL. The experiment was divided into 2 groups: experimental group P2 chondrocytes were transferred by rapid prototype biological printer (driving voltage value 50 V, interval in x-axis 300 μm, interval in y-axis 1500 μm). Afterwards Live/Dead viability Kit and flow cytometry were respectively adopted to detect cell viability; CCK-8 Kit was adopted to detect cell proliferation viability; immunocytochemistry, immunofluorescence and RT-PCR was employed to identify related markers of chondrocytes; control group steps were the same as the printing group except that cell suspension received no printing.\u0000\u0000\u0000RESULTS\u0000Fluorescence microscopy and flow cytometry analyses showed that there was no significant difference between experimental group and control group in terms of cell viability. After 7-day in vitro culture, control group exhibited higher O.D values than experimental group from 2nd day to 7th day but there was no distinct difference between these two groups (P>0.05). Inverted microscope observation demonstrated that the morphology of these two groups had no significant difference either. Similarly, Immunocytochemistry, immunofluorescence and RT-PCR assays also showed that there was no significant difference in the protein and gene expression of type II collagen and aggrecan between these two groups (P>0.05). Conclusion Cell printing has no distinctly negative effect on cell vitality, proliferation and phenotype of chondrocytes. Biological printing technique may provide a novel approach for realizing the oriented, quantificational and regular distribution of chondrocytes in a two-dimensional plane and lay the foundation for the construction of three-dimensional cell printing or even organ printing system.","PeriodicalId":13892,"journal":{"name":"International journal of clinical and experimental medicine","volume":"8 10 1","pages":"17471-9"},"PeriodicalIF":0.1,"publicationDate":"2015-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"64479747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimal sample volumes of human trabecular bone in μCT analysis within vertebral body and femoral head.","authors":"Xin-Xin Wen, Chun-Lin Zong, Chao Xu, Xiang-Yu Ma, Fa-Qi Wang, Ya-Fei Feng, Ya-Bo Yan, Wei Lei","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Trabecular bones of different skeletal sites have different bone morphologies. How to select an appropriate volume of region of interest (ROI) to reflect the microarchitecture of trabecular bone in different skeletal sites was an interesting problem. Therefore, in this study, the optimal volumes of ROI within vertebral body and femoral head, and if the relationships between volumes of ROI and microarchitectural parameters were affected by trabecular bone morphology were studied. Within vertebral body and femoral head, different cubic volumes of ROI (from (1 mm)(3) to (20 mm)(3)) were set to compare with control groups(whole volume of trabecular bone). Five microarchitectural parameters (BV/TV, Tb.N, Tb.Th, Tb.Sp, and BS/BV) were obtained. Nonlinear curve fitting functions were used to explore the relationships between the microarchitectural parameters and the volumes of ROI. The volumes of ROI could affect the microarchitectural parameters when the volume was smaller than (8 mm)(3) within the vertebral body and smaller than (13 mm)(3) within the femoral head. As the volume increased, the variable tendencies of BV/TV, Tb.N, and Tb.Sp were different between these two skeletal sites. The curve fitting functions between these two sites were also different. The relationships between volumes of ROI and microarchitectural parameters were affected by the different trabecular bone morphologies within lumbar vertebral body and femoral head. When depicting the microarchitecture of human trabecular bone within lumbar vertebral body and femoral head, the volume of ROI would be larger than (8 mm)(3) and (13 mm)(3). </p>","PeriodicalId":13892,"journal":{"name":"International journal of clinical and experimental medicine","volume":"8 10","pages":"17868-79"},"PeriodicalIF":0.1,"publicationDate":"2015-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4694281/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141081266","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association between sodium iodide symporter and differentiated Thyroid cancer: a meta-analysis of 9 studies.","authors":"Rui Zhang, Huanjun Wang, Junyu Zhao, Jinming Yao, Hongxia Shang, Huangao Zhu, Lin Liao, Jianjun Dong","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Context: </strong>As many studies proved that sodium iodide symporter (NIS) plays a key role in radioactive iodide (RAI) therapy of thyroid cancer, however, a growing number of studies suggests that part of differentiated thyroid carcinomas (DTC) with overexpression of NIS are insensitive to RAI well.</p><p><strong>Objective: </strong>The aim of this meta-analysis is to assess the expression of NIS in differentiated thyroid cancer, compared with normal thyroid tissue.</p><p><strong>Data sources: </strong>PUBMED, Sinomed, CNKI, Wanfang and VIP were searched for relevant case-control studies up to now.</p><p><strong>Study selection: </strong>Studies that concerning the qualitative expression NIS in DTC were included.</p><p><strong>Data extraction: </strong>Working independently, authors used a standard form to extract data. For quality assessment, Newcastle-Ottawa Scale (NOS) were applied.</p><p><strong>Data synthesis: </strong>Totally nine eligible studies included, involving 765 cases and 473 controls. The results revealed that the expression of NIS had a statistically increased in DTC, compared with controls (OddsRadio OR: 1.47, 95% CI: 1.12 to 1.94, Z=2.78, P=0.005). Since the existence of the significant heterogeneity, subgroup analysis and sensitivity analysis were performed and found that the heterogeneity came from the different criteria evaluate positive NIS expression (Liu 2008, Mu 2010) and the small simple size of the control group (Lin. J D2001). The heterogeneity disappeared or dropped to below 50% after remove these studies.</p><p><strong>Conclusion: </strong>Our study shows that the expression of NIS is significantly increased in DTC, which could help explain the reason for individual with a poor response to RAI therapy. In other word, the reduced iodide uptake in thyroid cancer may not caused by the decreased expression of NIS, function of NIS protein or its post-transcriptional translocation might be the point.</p>","PeriodicalId":13892,"journal":{"name":"International journal of clinical and experimental medicine","volume":"8 10","pages":"17986-94"},"PeriodicalIF":0.1,"publicationDate":"2015-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4694293/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140193698","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioactivity of umbilical cord blood dendritic cells and anti-leukemia effect.","authors":"Xu-Cang Wei, Di-Di Yang, Xiu-Rui Han, Yu-An Zhao, Yan-Chun Li, Li-Jie Zhang, Jiu-Ju Wang","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>We investigated the effect of umbilical cord blood dendritic cells (DCs) on in vitro proliferation, immunophenotypes and levels of homologous cytokine-induced killer cells (CIK) and the toxicity on leukemia cells.</p><p><strong>Method: </strong>Mononuclear cell-induced DC-CIK cells derived from umbilical cord blood were collected and co-cultured in the proportion of 1:5. Cord blood CIK cells or peripheral blood DC-CIK cells were used as control. Phenotypes were analyzed by flow cytometry; vial cell counting was performed using trypan blue, and the killing activity of effector cells against leukemia cells was measured by MTT assay. The levels of interferon-r (IFN-r), tumor necrosis factor-a (TNF-α) and interleukin-12 (IL-12) were determined by ELISA.</p><p><strong>Results: </strong>The proliferative capacity of DC-CIK cells was obviously improved compared with cord blood CIK cells and peripheral blood DC-CIK cells (P<0.05, P<0.05). During the co-culture of cord blood DC-CIK cells, the ratios of CD 3 (+) CD 8 (+) and CD 3 (+) CD 56 (+) cells were obviously higher than that of CIK cells under the same conditions (P<0.05). On day 3 of co-culture, the levels of IL-12, IFN-r and TNF-a in cultured supernatant of cord blood DC-CIK cells were all higher than those secreted by CIK cells cultured alone (P<0.01, P<0.05, P<0.05). When the effector to target ratio was 2.5-20:1, the killing effect of cord blood DC-CIK cells against each subtype of acute leukemia cells was obviously higher than that of CIK cells (P<0.05). No significant differences in killing effect were observed for different subtypes. This finding was consistent with the killing effect of peripheral blood DC-CIK cells against leukemia cells.</p><p><strong>Conclusion: </strong>Cord blood DCs can enhance the proliferative capacity of homologous CIK cells and its anti-leukemia effect. Though cord blood DC-CIK cells showed a higher proliferative capacity than peripheral blood DC-CIK cells, the two types of DC-CIK cells did not differ significantly in terms of cytoxicity. With a high availability and the low probability of graft rejection reaction, cord blood DC-CIK cells have a brighter prospect for application in immunotherapy.</p>","PeriodicalId":13892,"journal":{"name":"International journal of clinical and experimental medicine","volume":"8 10","pages":"19725-30"},"PeriodicalIF":0.1,"publicationDate":"2015-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4694537/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141081259","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficacy of silodosin in the treatment of distal ureteral stones 4 to 10 mm in diameter.","authors":"Mustafa Yuksel, Serdar Yilmaz, Husnu Tokgoz, Soner Yalcinkaya, Serkan Baş, Tümay Ipekci, Ali Yildiz, Nihat Ates, Murat Savas","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Purpose: </strong>Few studies have investigated the efficacy of silodosin, a recently introduced selective alpha 1-A adrenoceptor antagonist, in medical expulsive therapy (MET) for ureteral calculi. The results of these studies, which all evaluated the efficacy of 8 mg/day, indicate that silodosin is a potential treatment for ureteral calculi. This study investigated the efficacy of 4 mg/day of silodosin for MET of distal ureteral stones 4 to 10 mm in diameter.</p><p><strong>Material and method: </strong>After 70 patients had been randomized into 2 groups of 35 patients each, both the control and experimental groups (groups 1 and 2, respectively) were advised to take 75 mg/day of diclofenacsodiumas needed for pain relief but only the experimental group to take 4 mg/day of silodosin. After 21 days, the groups were compared regarding the stone expulsion rate and duration, number of renalcolicepisodes, and analgesicdosage.</p><p><strong>Results: </strong>The median expulsion rates were 71.4% and 91.4% in groups 1 and 2, respectively, and the difference between them was significant (P=0.031). The median expulsion durations were 12.91±6.14 and 8.03±4.99 days, respectively, and the difference between them was significant (P<0.001). No significant differences were found regarding the median number of renal colic episodes or median analgesic dosage. While no patients in group 1 experienced side effects, 5 patients (14%) in group 2 experienced retrograde ejaculation.</p><p><strong>Conclusion: </strong>These results indicate that 4 mg/day of silodos in facilitates the expulsion of distal ureteral stones 4 to 10 mm in diameter but does not significantly reduce the number of renal colic episodes or analgesic dosage.</p>","PeriodicalId":13892,"journal":{"name":"International journal of clinical and experimental medicine","volume":"8 10","pages":"19086-92"},"PeriodicalIF":0.1,"publicationDate":"2015-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4694437/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140193699","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In vitro and in vivo effects of ethanol extract combined with Curcumae Radix and Glycyrrhizae Radix et Rhizoma on menopausal metabolic disturbances.","authors":"InSil Park, JinAh Ryuk, HyeWon Lee, Hiroe Go, ByoungSeob Ko","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Curcumae Radix (CR) and Glycyrrhizae Radix et Rhizoma (GR) extracts have been used as health supplements in traditional medicine. This study was performed to evaluate the effects of combined CR and GR extracts (CR+GR) on metabolic complications related menopausal symptoms. We found a significant results that CR+GR extracted using ethanol stimulated the growth of MCF-7 cells in estrogen activity and was attenuated in lipid deposition of HepG2 cells treated with MβCD compared to CR and GR treatments each. To investigate the situation, an experimental menopause rat model with dyslipidemia was induced by surgical bilateral ovariectomy (OVX) and high fat high cholesterol (HFHC) diet in female rats. OVX rats fed HFHC (OVX-HFHC) showed a shift in weight gain, elevated serum cholesterol, altered liver enzymatic parameters and enhanced liver injury compared to the NC and HFHC groups. However, administration of CR+GR, in particular 200 or 450 mg/kg/day, inhibited the increase in body weight gain and lipid metabolic disturbances, lowering total cholesterol (TC), triglycerides (TG) and low density lipoprotein cholesterol (LDL-C) compared to the OVX-HFHC group. Furthermore, CR+GR (200 or 450 mg/kg/day) ameliorated the serum levels of the liver enzymes aspartate aminotransferase (AST) and alanine transaminase (ALT) compared to the OVX-HFHC group. Moreover, CR+GR (200 or 450 mg/kg/day) attenuated not only hepatic steatosis but also larger adipocytes. Our study demonstrated that combined treatment with CR and GR attenuated metabolic complications induced by OVX and HFHC diet, suggesting that this effect may regulate and prevent the acceleration of cardiovascular disease (CVD) after menopause. </p>","PeriodicalId":13892,"journal":{"name":"International journal of clinical and experimental medicine","volume":"8 9","pages":"15076-86"},"PeriodicalIF":0.1,"publicationDate":"2015-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4658880/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140193722","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The diagnostic value of neutrophil gelatinase-associated lipocalin and hepcidin in bacteria translocation of liver cirrhosis.","authors":"Jiangguo Zhang,&nbsp;Fengyun Gong,&nbsp;Ling Li,&nbsp;Manzhi Zhao,&nbsp;Zhuhua Wu,&nbsp;Jianxin Song","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>Bacterial translocation (BT) or bacterial DNA (bactDNA) translocation is a critical pathogenesis mechanism of spontaneous bacterial peritonitis. Studies of BT or bactDNA translocation are limited in humans. Neutrophil gelatinase associated lipocalin (NGAL) can efficiently distinguish bacterial and nonbacterial ascites in ascitic patients. Hepcidin is a useful marker of bacterial infection in the late-onset sepsis. However, the relationship between NGAL, hepcidin and BT was still unclear. In present study, the levels of NGAL, hepcidin and their relationship with BT or bactDNA translocation were investigated.</p><p><strong>Material and methods: </strong>Weekly doses of carbon tetrachloride (CCl4) were given to induce liver cirrhosis in Sprague-Dawley rats. Trypticase (blood) soy agars were used to culture bacteria. BactDNA was sequenced by ABIPRISM 310 automated sequencer. The levels of NGAL and hepcidin were assessed by ELISA. Receiver operating characteristic (ROC) curve was used to determine the cut-off values and compare the diagnostic performance of NGAL and hepcidin.</p><p><strong>Results: </strong>56 cirrhotic and 10 normal rats were included in this study. The levels of both two biomarkers were significantly higher in BT or bactDNA translocation group compared to non-translocation group. The area under ROC curve for the diagnosis of BT was 0.910 for serum NGAL, 0.858 for serum hepcidin and 0.940 for their combination, whereas that for the diagnosis of bactDNA translocation was 0.906 for NGAL, 0.779 for hepcidin and 0.950 for their combination, respectively. The combination of NGAL and hepcidin improved the ability to detect BT or bactDNA presence in MLNs and ascites.</p><p><strong>Conclusion: </strong>BT and the presence of bactDNA in MLNs were observed in a rat cirrhotic model. Serum NGAL and hepcidin can serve as sensitive and specific tests for diagnosis of BT or bactDNA translocation. NGAL in combination with hepcidin can improve the accuracy of diagnosis.</p>","PeriodicalId":13892,"journal":{"name":"International journal of clinical and experimental medicine","volume":"8 9","pages":"16434-44"},"PeriodicalIF":0.1,"publicationDate":"2015-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4659057/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71434226","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}