K. Rai, Apoorva, Lakee Sharma, N. Pandey, R. Meena, S. Rai
{"title":"Repurposing Artemisia annua L. Flavonoids, Artemisinin and Its Derivatives as Potential Drugs Against Novel Coronavirus (SARS –nCoV) as Revealed by In-Silico Studies","authors":"K. Rai, Apoorva, Lakee Sharma, N. Pandey, R. Meena, S. Rai","doi":"10.3126/ijasbt.v8i4.33667","DOIUrl":"https://doi.org/10.3126/ijasbt.v8i4.33667","url":null,"abstract":"This paper can be downloaded online at http://ijasbt.org & http://nepjol.info/index.php/IJASBT 374 Repurposing Artemisia annua L. Flavonoids, Artemisinin and Its Derivatives as Potential Drugs Against Novel Coronavirus (SARS –nCoV) as Revealed by In-Silico Studies Krishna Kumar Rai, Apoorva, Lakee Sharma, Neha Pandey, Ram Prasad Meena, Shashi Pandey Rai Laboratory of Morphogenesis, Centre of Advance Study in Botany, Department of Botany, Institute of Science, Banaras Hindu University (BHU), Varanasi-221005 (Uttar Pradesh), India Department of Botany, CMP PG College, University of Allahabad, Prayagraj, India","PeriodicalId":13876,"journal":{"name":"International Journal of Applied Sciences and Biotechnology","volume":"40 1","pages":"374-393"},"PeriodicalIF":0.0,"publicationDate":"2020-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79019187","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Deleterious Effects of Storage Environmental Conditions on The Seed Quality of Two Varieties of Tomato (Solanum lycopersicum L.) Stored in Triple Laminated Aluminum Packing in Sri Lanka","authors":"R. Ariyarathna, S. Weerasena, C. Beneragama","doi":"10.3126/ijasbt.v8i4.33670","DOIUrl":"https://doi.org/10.3126/ijasbt.v8i4.33670","url":null,"abstract":"This is an open access article & it is licensed under a Creative Commons Attribution Non-Commercial 4.0 International (https://creativecommons.org/licenses/by-nc/4.0/) Tomato is one of the most commonly growing vegetable crops among the farmers in Sri Lanka. Standard laboratory germination of tomato seeds fulfills the regulatory requirements of seed marketing. However, poor field performance is an overwhelming problem to farmers. Present study was focused on the longevity of two varieties of tomato seeds as affected by their quality characters (percentage germination, moisture, field emergence and vigour index, seed protein and carbohydrate contents) including seed health (exposure of Clavibacter michiganensis subsp. Michiganensis (CMMV), Spotted wilt virus (TSWV) and Leaf curly top virus (LCTV)) under four different storage environmental conditions during a one-year long storage study. Seeds were packed in triple-laminated aluminum packets (TLA) and stored in low temperature storage conditions (17±1 °C and 65% RH) and in ambient conditions in Gannoruwa, Kundasale, and MahaIlluppallama that represent the three agro-ecologically zones; Mid Country Wet Zone (MCWZ), Mid Country Intermediate Zone (MCIZ) and Low Country Dry Zone (LCDZ) respectively. Seed quality parameters displayed varied responses depending on the variety. Physiological changes that occur in seeds due to temperature, RH, changes in proteins and carbohydrate content, CMMV and TSWV during storage were manifested as reduction in seed germination, field emergence and vigour index. However, none of the seedling carried LCTV and therefore its effect on seed quality parameters could not be assessed. The seeds could be stored for ca. 29 months at low and constant temperatures and RH conditions without compromising local seed germination standards compared to ambient storage in the three environments. CMMV and TSWV detected in all seed samples indicated potential threats to tomato farmers in Sri Lanka.","PeriodicalId":13876,"journal":{"name":"International Journal of Applied Sciences and Biotechnology","volume":"21 1","pages":"437-447"},"PeriodicalIF":0.0,"publicationDate":"2020-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75077104","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Farmers' Preferences of Improved Wheat Varieties in Wheat Subsector Kailali, Nepal","authors":"P. Bhatt, P. Bist, L. N. Ojha","doi":"10.3126/ijasbt.v8i4.33671","DOIUrl":"https://doi.org/10.3126/ijasbt.v8i4.33671","url":null,"abstract":"This is an open access article & it is licensed under a Creative Commons Attribution Non-Commercial 4.0 International (https://creativecommons.org/licenses/by-nc/4.0/) Kailali is the second highest district regarding wheat production and area coverage. A study was carried out in the Kailali district for preference of farmers on different wheat varieties that were being cultivated in the district. The objective of the study was to assess farmers’ preference and perception on the different improved wheat varieties in the area. Five commonly grown wheat varieties and seventeen important wheat varietal traits were selected through focus group discussion, key informant interview and preliminary survey. Sixty households were selected for face-to-face personal interview, twelve for each selected variety, at random from Ghodaghodi Municipality, Gauriganga Municipality and Kailari Rural Municipality of Kailali. The weightage of the seventeen selected traits were determined using an online survey conducted among individuals from different designation with the agricultural background who were well known about wheat cultivation practices in Kailali district. Yield, flour quality and tillering were found to be the trait of higher weightage and stem thickness was the trait of lowest weightage. The preferences of the farmers were measured using wheat preference index (WPI). The varieties Vijay, NL-971, HD-2967, Gautam and Aditya were ranked as I, II, III, IV and V respectively. The incorporation of farmers' preferred traits in future wheat varieties would increase the likelihood of adoption of the varieties. Similarly, proper participatory field trial of new improved wheat varieties would also help in the selection of the suitable varieties in the area concerning the farmers’ criteria of selection.","PeriodicalId":13876,"journal":{"name":"International Journal of Applied Sciences and Biotechnology","volume":"25 1","pages":"432-436"},"PeriodicalIF":0.0,"publicationDate":"2020-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74677475","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Wild Edible Fruits of Nepal","authors":"R. S. Gautam, S. Shrestha, Ila Shrestha","doi":"10.3126/IJASBT.V8I3.31561","DOIUrl":"https://doi.org/10.3126/IJASBT.V8I3.31561","url":null,"abstract":"This is an open access article & it is licensed under a Creative Commons Attribution Non-Commercial 4.0 International (https://creativecommons.org/licenses/by-nc/4.0/) A review was done on the wild edible fruit plants of Nepal. Altogether 199 wild edible fruit plant species belonging to 139 genera representing 67 families documented from west Nepal to east Nepal. Study shows that people are using the wild edible fruits collected from forest resources as a part of their livelihood. People consume those wild fruits in different forms such as raw fruit, vegetables, pickles, spices and oil. Some species are taken in more than one form.","PeriodicalId":13876,"journal":{"name":"International Journal of Applied Sciences and Biotechnology","volume":"116 1","pages":"289-304"},"PeriodicalIF":0.0,"publicationDate":"2020-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90991489","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Constraints and Determinants of Coffee Processing Methods in Gulmi District, Nepal","authors":"Manoj Paudel, K. Parajuli","doi":"10.3126/IJASBT.V8I3.31565","DOIUrl":"https://doi.org/10.3126/IJASBT.V8I3.31565","url":null,"abstract":"This is an open access article & it is licensed under a Creative Commons Attribution Non-Commercial 4.0 International (https://creativecommons.org/licenses/by-nc/4.0/) Nepalese coffee is widely recognized as organic and specialty coffee due to its peculiar aroma and flavor. Altitude, geographical location, orchard management practices, post-harvest handling are the major factors that affect the coffee quality. Coffee quality is determined 40% in the field, 40% at postharvest primary processing, and 20% at secondary processing. In Nepal, dry and wet methods are the most common coffee processing methods. Wet processed coffee is more preferred by the consumers in comparison to dry coffee and commands higher price. Main objective of the study was to know the most common processing method adopted in Gulmi district and to know the determinants and constraints of the processing method. 100 coffee growers from four different local bodies of Gulmi district were selected by the purposive sampling method for the household survey. Descriptive statistics, logit model and index score ranking method were used for the data analysis. Gender of household head (p <0.01), education level (p<0.05) and technical trainings (p<0.05) were found to have significant effect upon the selection of processing methods. Due to several constraints all coffee growers were unable to adopt wet processing of coffee. Index score method was used to rank the constraints for adoption of wet processing methods. Lack of availability of hand pulpers was ranked as the major constraint by the coffee growers with an index score of 0.867 followed by unavailability of water, no access to transportation as other major constraints for adoption of wet processing method.","PeriodicalId":13876,"journal":{"name":"International Journal of Applied Sciences and Biotechnology","volume":"28 1","pages":"368-373"},"PeriodicalIF":0.0,"publicationDate":"2020-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75022671","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Isolation and Screening of Polyhydroxyalkanoates (PHA) Producing Bacteria Utilizing Agricultural Waste","authors":"I. Afghan, A. Shrivastav","doi":"10.3126/IJASBT.V8I3.31566","DOIUrl":"https://doi.org/10.3126/IJASBT.V8I3.31566","url":null,"abstract":"This paper can be downloaded online at http://ijasbt.org & http://nepjol.info/index.php/IJASBT 336 Isolation and Screening of Polyhydroxyalkanoates (PHA) Producing Bacteria Utilizing Agricultural Waste Inzer Gul Afghan1, Anupama Shrivastav Education Faculty, Chemistry department, Paktia University, Gardez, Paktia, Afganistan Parul Institute of Applied sciences, Microbiology department, Parul University, Limda, Waghodiya, Vadodara, India","PeriodicalId":13876,"journal":{"name":"International Journal of Applied Sciences and Biotechnology","volume":"1 1","pages":"336-342"},"PeriodicalIF":0.0,"publicationDate":"2020-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91018771","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Response of Nutrient Omission and Irrigation Scheduling on Growth and Productivity of Maize","authors":"N. Acharya, S. Sah, A. K. Gautam, A. Regmi","doi":"10.3126/IJASBT.V8I3.31612","DOIUrl":"https://doi.org/10.3126/IJASBT.V8I3.31612","url":null,"abstract":"This paper can be downloaded online at http://ijasbt.org & http://nepjol.info/index.php/IJASBT 343 Response of Nutrient Omission and Irrigation Scheduling on Growth and Productivity of Maize N.R. Acharya, S.K. Sah, A.K. Gautam, A.P. Regmi Nepal Agricultural Research Council, Directorate of Agricultural Research, Khajura, Banke, Nepal Agriculture and Forestry University, Rampur, Chitwan, Nepal Nepal Agricultural Research Council, Singh Durbar Plaza, Kathmandu, Nepal","PeriodicalId":13876,"journal":{"name":"International Journal of Applied Sciences and Biotechnology","volume":"26 1","pages":"343-354"},"PeriodicalIF":0.0,"publicationDate":"2020-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84408140","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Applications of Biotechnology for Characterization of Plants and Pests as the Key Components of Plant Protection and Production Strategies: A Review","authors":"M. Alemu","doi":"10.3126/IJASBT.V8I3.31556","DOIUrl":"https://doi.org/10.3126/IJASBT.V8I3.31556","url":null,"abstract":"This is an open access article & it is licensed under a Creative Commons Attribution Non-Commercial 4.0 International (https://creativecommons.org/licenses/by-nc/4.0/) The second Sustainable Developmental Goals (SDG), among the seventeen SDG, is concerned with the pursuit of global food security and agricultural sustainability, which become the key to the success of the entire SDG. Whereas agricultural production and productivity are heavily threatened by the incidence of pests that inflict huge losses in various forms. This calls for prompt applications of biotechnology for the fast, accurate and reliable means for characterization of plant generic resources and pests as it is the pre-requisite and gateway for designing appropriate plant protection and production strategies. It is imperative that pests be identified properly so that judicious use of the literature can be made and sustainable management strategies can be implemented at the right stage. To this end, the application of biotechnology has made significant advances for reliable characterization of plant genetic resources as well as accurate diagnosis of pests, study of their genetic diversity and variability, detailed mechanisms of their transmission and evolutionary relationships. Accordingly, this review article covers the comprehensive account of the various molecular techniques, genome mapping and OMICS sciences utilized for characterization plants and pests that ultimately allow the detailed study of the biology and epidemiology of pests at any stage of their life cycle. The resulting data are eventually employed for enhancing successful implementation of sustainable plant protection and production strategies. In conclusion, the increasing projections of transboundary pests, environmental and abiotic factors together with the continuous scientific advancements and breakthroughs have made biotechnology to be an important engine of bioeconomy for generating invaluable products, processes and services.","PeriodicalId":13876,"journal":{"name":"International Journal of Applied Sciences and Biotechnology","volume":"10 1","pages":"247-288"},"PeriodicalIF":0.0,"publicationDate":"2020-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82265848","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Seroprevalence of Chlamydia abortus Among Cattle of Selected Areas in Terai Belt of Nepal","authors":"R. Kandel, D. Subedi, C. N. Kharel","doi":"10.3126/IJASBT.V8I3.30235","DOIUrl":"https://doi.org/10.3126/IJASBT.V8I3.30235","url":null,"abstract":"This paper can be downloaded online at http://ijasbt.org & http://nepjol.info/index.php/IJASBT 363 Seroprevalence of Chlamydia abortus Among Cattle of Selected Areas in Terai Belt of Nepal Rupak Kandel, Deepak Subedi, Chet Narayan Kharel Paklihawa Campus, Institute of Agriculture and Animal Science, Tribhuvan University, Rupandehi, Nepal New Hope Agro Business Nepal Private Limited, Bharatpur, Chitwan, Nepal National Avian Disease Investigation Laboratory, Bharatpur, Chitwan, Nepal","PeriodicalId":13876,"journal":{"name":"International Journal of Applied Sciences and Biotechnology","volume":"80 1 1","pages":"363-367"},"PeriodicalIF":0.0,"publicationDate":"2020-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83514432","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Mustard Aphid and Crop Production","authors":"Saurabha Koirala","doi":"10.3126/IJASBT.V8I3.31558","DOIUrl":"https://doi.org/10.3126/IJASBT.V8I3.31558","url":null,"abstract":"This is an open access article & it is licensed under a Creative Commons Attribution Non-Commercial 4.0 International (https://creativecommons.org/licenses/by-nc/4.0/) Mustard aphid, Lipaphis erysimi (L.) Kaltenbach (Homoptera: Aphididae) is the most notorious, cosmopolitan louse-like and obligate ectoparasite, which causes a bulk of the qualitative and quantitative loss of rapeseed-mustard crops. This article reviews the general overview of biology, damages, and the management of mustard aphids. Biologically, L. erysimi adults are soft bodies, varying in color mostly yellowish-greenish, small to medium-sized, globular, pear-shaped, manifesting wing dimorphism based on the resource availability. Plasticity in reproductive mode either sexually or asexually stimulated as an adaptive response to cope with seasonal fluctuations, maximizes the chance of survival from predators being outnumbering. Mustard aphid infested host plant in three major ways, firstly by sucking the plant phloem through stylets, the needle-like piercing-sucking mouthparts which manifest injury like curling and yellowing of the leaf, stunting and drying up of the plants. Secondly, by excreting a sticky substance (honeydew) on which 'sooty mold' growth, which blocks the process of photosynthesis. Thirdly, it causes secondary plant injury by transmission and dissemination of viruses including turnip mosaic viruses. The appearance, multiplication, and disappearance of mustard aphid are largely regulated by weather variations. Its prolific multiplication greatly thrives by cool, wet, and cloudy weather. The mustard aphid can be eschewed more so if the crop is sown before 20th October. Applications of the recommended dose of fertilizers, irrigation, resistant varieties are some cultural practices to cope with the aphid population. Natural enemies are effective and impressive nowadays for crop protection. Ladybird beetles viz., Cocciniella septempunctata, Hippodamia variegata, and Cheilomones vicina are active predators of this pest. Chemical control includes the application of systemic insecticides below the ETL (Economic Threshold Level). Some major insecticide includes are Imidacloprid 17.8% @ 0.25 ml/l, Thiamethoxam 25 WG @ 0.2g/l and Dimethoate 30EC @ 1 ml/l of water.","PeriodicalId":13876,"journal":{"name":"International Journal of Applied Sciences and Biotechnology","volume":"1 1","pages":"310-317"},"PeriodicalIF":0.0,"publicationDate":"2020-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75765434","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}