International Journal of Applied Sciences and Biotechnology最新文献_第9页

Prioritised Zoonotic Diseases of Nepal: A Review 尼泊尔重点人畜共患疾病:综述

International Journal of Applied Sciences and Biotechnology Pub Date : 2021-03-30 DOI: 10.3126/IJASBT.V9I1.34967

Anil Gautam, Pramod Upadhayay, Devendra Ghimre, A. Khanal, Asmita Gaire, K. Kaphle

引用次数: 0

Heterobeltiosis and Economic Heterosis for Grain Yield Related Traits of Boro Rice (Oryza sativa L.) 水稻产量相关性状的杂种优势和经济优势

International Journal of Applied Sciences and Biotechnology Pub Date : 2021-03-30 DOI: 10.3126/IJASBT.V9I1.34038

Shuma Rani Ray, A. Islam, M. Rasul, M. Saikat, J. Ahmed

引用次数: 3

In-Vitro Evaluation of Biofilm and Hemolysis activity of Candida albicans Isolated from Oral Cavity 口腔分离白色念珠菌生物膜和溶血活性的体外评价

International Journal of Applied Sciences and Biotechnology Pub Date : 2020-12-28 DOI: 10.3126/ijasbt.v8i4.32971

B. Shrestha, J. Shakya

{"title":"In-Vitro Evaluation of Biofilm and Hemolysis activity of Candida albicans Isolated from Oral Cavity","authors":"B. Shrestha, J. Shakya","doi":"10.3126/ijasbt.v8i4.32971","DOIUrl":"https://doi.org/10.3126/ijasbt.v8i4.32971","url":null,"abstract":"Candida albicans is a member of the healthy human microflora, colonizing several niches in the body and can cause opportunistic infection under host debilitated and immunocompromised condition. The present study aimed to investigate the in-vitro hemolytic activity of C. albicans isolated from oral cavity and screen biofilm through three different methods. During the study, 200 oral rinse samples from general human population were analyzed in microbiology laboratory of Central Campus of Technology, Tribhuvan University, Hattisar, Dharan. Nepal. Candida albicans were isolated and identified by conventional microbiological procedures. The hemolytic activity was evaluated through two different Sabouraud dextrose broth media (SDB) containing 7% defibrinated human blood, one supplemented with 3% glucose (SDBwG) and the other without glucose (SDBwoG). The biofilm formation was screened through congo red agar, tube method and tissue culture plate method. In this present study, 42 (21%) isolates of Candida albicans were isolated from 200 oral rinse samples. Isolated Candida albicans exhibited mean hemolysis activity of 28.66% on human blood SDB without glucose and 43.55% on human blood SDB with 3% glucose. Tissue culture plate method was considered sensitive, specific and accurate method for quantitative screening of biofilm in comparison to tube method and congo red agar method. This research concluded that Candida albicans exhibited greater hemolytic activity in human blood with glucose (SDBwG) than in human blood without glucose (SDBwoG). This finding explains that an increased blood glucose concentration may contribute to increased hemolysis activity of Candida albicans that could play pathogenic role for inducing infection like oral candidiasis in debilitated host like diabetic patients. Tissue culture plate method can accurately screen biofilms than tube and congo red agar method. \u0000Int. J. Appl. Sci. Biotechnol. Vol 8(4): 394-399","PeriodicalId":13876,"journal":{"name":"International Journal of Applied Sciences and Biotechnology","volume":"74 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78280226","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Molecular Identity of Subterranean Termites 地下白蚁的分子特性

International Journal of Applied Sciences and Biotechnology Pub Date : 2020-12-28 DOI: 10.3126/ijasbt.v8i4.32445

Srinivasa Murthy Kotilingam

{"title":"Molecular Identity of Subterranean Termites","authors":"Srinivasa Murthy Kotilingam","doi":"10.3126/ijasbt.v8i4.32445","DOIUrl":"https://doi.org/10.3126/ijasbt.v8i4.32445","url":null,"abstract":"This is an open access article & it is licensed under a Creative Commons Attribution Non-Commercial 4.0 International (https://creativecommons.org/licenses/by-nc/4.0/) Identification of termites is a challenging task due to variable morphological differences among species and their eusocial behaviour with different caste system. A method for easy and accurate species-level identification at any life stage is required. In this study, a 658-base pair region of the mitochondrial cytochrome oxidase I (COI) gene was used to explore its utility in the identification of important subterranean termites. Termite specimens collected from 25 locations from various states, were characterized using specific primers for their identification. Sequence analysis and divergence among the species was assessed. The composition of the mitochondrial sequence of the COI gene in the present study was expectedly AT biased. GenBank accession numbers were obtained for the species. Sequence analysis and divergence among the species was assessed, which revealed significantly high percentage of A+T base composition. Molecular sequence information from NCBI revealed relatedness in all the collected termites, accurately as revealed by their morphological characters. The studies indicate the relevance of DNA sequencing to match different forms of termites and address ambiguities in morphological identification. The information on species diversity would help plan strategies for management of termites.","PeriodicalId":13876,"journal":{"name":"International Journal of Applied Sciences and Biotechnology","volume":"20 1","pages":"410-416"},"PeriodicalIF":0.0,"publicationDate":"2020-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86901942","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Evaluation and Release of High Yielding and High Oil Containing Mustard for Terai and Inner Terai of Nepal 尼泊尔特赖和内特赖高产高含油芥菜的评价与释放

International Journal of Applied Sciences and Biotechnology Pub Date : 2020-12-28 DOI: 10.3126/ijasbt.v8i4.33825

B. Yadav, Santosh Rasaily, P. Wagle, R. Malla, M. Kandel, B K Nabina, Sheetal Aryal

{"title":"Evaluation and Release of High Yielding and High Oil Containing Mustard for Terai and Inner Terai of Nepal","authors":"B. Yadav, Santosh Rasaily, P. Wagle, R. Malla, M. Kandel, B K Nabina, Sheetal Aryal","doi":"10.3126/ijasbt.v8i4.33825","DOIUrl":"https://doi.org/10.3126/ijasbt.v8i4.33825","url":null,"abstract":"In Recent years, cultivation of Mustard (Brassica juncea) is growing popularity but there is lack of suitable high yielding and oil contenting variety in Nepal. With Objective of identifying and releasing of high yielding variety, evaluation of exotic genotypes were done in the research field and farmers field. Coordinated Varietal Trial (CVT) was conducted for three years in three different location (Nawalpur, parwanipur and Khajura) in RCBD design. 4 Elite genotypes were selected and further evaluate under Coordinated Farmer Field Trial (CFFT) for 5 years where each farmer was considered as Replication. Nutrients Analysis was done to find out the moisture, oil and protein content of selected elite genotypes. In combined ANNOVA of CVT, ICJ 9704 was found to be high yielding genotypes. Similarly, In CFFT, genotypes was significantly for yield where ICJ 9704 produced highest yield (906.75 kg/ha) where as Divya, Pusa Jagarnath and Krishna produced yield of 798.12 kg/ha, 790 kg/ha and 767.392 kg/ha. ICJ 9704 has high oil content 37.3 % with 36.3 % protein content. In 2017, ICJ 9704 was release as Morang rayo and was recommended to grow in the terai and inner terai. Hope this variety will be popular among mustard growing farmer and will help to meet the national demand of the Nation. \u0000 Int. J. Appl. Sci. Biotechnol. Vol 8(4): 454-456","PeriodicalId":13876,"journal":{"name":"International Journal of Applied Sciences and Biotechnology","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82415333","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nutritional Value with Quality of Late Season Cauliflower Varieties in Terai Region of Nepal 尼泊尔德莱地区晚季花椰菜品种的营养价值与品质

International Journal of Applied Sciences and Biotechnology Pub Date : 2020-12-28 DOI: 10.3126/ijasbt.v8i4.33673

H. N. Giri

{"title":"Nutritional Value with Quality of Late Season Cauliflower Varieties in Terai Region of Nepal","authors":"H. N. Giri","doi":"10.3126/ijasbt.v8i4.33673","DOIUrl":"https://doi.org/10.3126/ijasbt.v8i4.33673","url":null,"abstract":"This is an open access article & it is licensed under a Creative Commons Attribution Non-Commercial 4.0 International (https://creativecommons.org/licenses/by-nc/4.0/) An experiment was carried out to assess the nutritional value along with quality of curds in late season cauliflower varieties at Rampur, Chitwan during November 2017 to March, 2018. The eleven late season cauliflower varieties were Freedom, Titan, Ravella, Amazing, Artica, Bishop, Casper, Indam 9803, NS 106, Snow Mystique and Snowball 16 which were introduced from USA, Europe, India and Nepal. The experiment was prepared in a Randomized Complete Block Design (RCBD) with four replications. The main objective of this study was to check the nutritional content, taste, compactness, appearance and freshness of the curds in eleven late season cauliflower varieties at Rampur, Chitwan. Nutritional value along with taste quality, compactness, appearance and freshness of the curds were measured after harvest. Maximum moisture and carbohydrate content was found in Freedom while higher crude fat was obtained in Titan and Bishop. Similarly, higher crude protein and crude fiber was found in NS 106. Significantly better taste score (8.8) was found in Snowball 16 than other varieties. Better compactness score of curd was found in Artica and Titan than other varieties. Significantly better appearance score (8.0) was found in Artica but poor appearance score was found in Casper than other varieties. At different intervals after harvesting of curds, significantly fresher curds were found in Artica than other varieties. In conclusion, NS 106 performed higher content of crude protein and crude fiber while Snowball 16 executed better tasty curds than other varieties. Artica also showed better performance on freshness of curds than other varieties.","PeriodicalId":13876,"journal":{"name":"International Journal of Applied Sciences and Biotechnology","volume":"2169 1","pages":"427-431"},"PeriodicalIF":0.0,"publicationDate":"2020-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91380513","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Production of Restorer Lines from Segregating Progenies of Brassica napus L. Having Good Agronomic Value 甘蓝型油菜分离后代具有良好农艺价值的恢复系生产

International Journal of Applied Sciences and Biotechnology Pub Date : 2020-12-28 DOI: 10.3126/ijasbt.v8i4.31352

A. Islam, F. Era, N. Chowdhury

{"title":"Production of Restorer Lines from Segregating Progenies of Brassica napus L. Having Good Agronomic Value","authors":"A. Islam, F. Era, N. Chowdhury","doi":"10.3126/ijasbt.v8i4.31352","DOIUrl":"https://doi.org/10.3126/ijasbt.v8i4.31352","url":null,"abstract":"This is an open access article & it is licensed under a Creative Commons Attribution Non-Commercial 4.0 International (https://creativecommons.org/licenses/by-nc/4.0/) Forty-four testcross progenies obtained from crossing between five CMS and forty-one candidate lines were evaluated both in field and laboratory condition to identify candidate restorer line(s). Nineteen testcross progenies were recorded as fully fertile with 100% plant fertility by counting the number of fertile plants from the total number of plants per lines. On the other hand, from pollen fertility test, 11 testcross progenies [206A × 001(6), 9905A × 030(2), 9905A × 027(6) (0.57), 206A × 37(1) (0.68), 9904A × 027(4) (0.83), 248A × 020(6) (1.08), 248A × 018 (1.12), 248A × 022 (1.13), 248A × 017 (1.58), 248A × 038(2) (1.96) and 248A × 001(6) (2.02)] were found with 0-2.02% pollen sterility that could be mentioned as fertile or restorer lines for making hybrids. Agronomic performances were also satisfactory for these selected test cross progenies. The genotype 248A × 017 took the shortest time (30.00) for first flowering as well as ripening followed by 248A × 007(1). The highest number of pods per plant was observed in the testcross progeny 248A × 022 (649.59) and the highest number of seeds per pod (30.33) in 248A × 020(6). For seed weight per plant, 206A × 001(6) was recorded with maximum value (0.08g) while the lowest (23.33) number of seeds per plant was found in this progeny. In case of seed yield per plant, 248A × 022 gave the highest yield (30.30). The seed yield of the progenies 248A × 017 (23.49), 9905A × 027(6) (20.39), 248A × 001(6) (17.26), 9904A × 027(4) (17.02) and 248A × 038(2) (16.53) were also in the highest level.","PeriodicalId":13876,"journal":{"name":"International Journal of Applied Sciences and Biotechnology","volume":"55 1","pages":"400-409"},"PeriodicalIF":0.0,"publicationDate":"2020-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87352660","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Karyomorphology of Two Species of the Genus Spilanthes (Heliantheae-Asteraceae) 菊科绣球花属两种植物的核形态研究

International Journal of Applied Sciences and Biotechnology Pub Date : 2020-12-28 DOI: 10.3126/ijasbt.v8i4.33666

P. K. Mallick

{"title":"Karyomorphology of Two Species of the Genus Spilanthes (Heliantheae-Asteraceae)","authors":"P. K. Mallick","doi":"10.3126/ijasbt.v8i4.33666","DOIUrl":"https://doi.org/10.3126/ijasbt.v8i4.33666","url":null,"abstract":"This is an open access article & it is licensed under a Creative Commons Attribution Non-Commercial 4.0 International (https://creativecommons.org/licenses/by-nc/4.0/) In present investigation karyotype of mitotic chromosomes were determined, two species of the genus Spilanthes, namely Spilanthes acmella (L.) Murray and Spilanthes calva DC. from plants transplanted in earthen pots collected from different localities from Central Nepal in my home garden. The somatic chromosome number determined in present study were 2n=36 for both species, Spilanthes acmella (L.) Murray and Spilanthes calva DC. The chromosomes total length ranged from 0.6 to 1.6 μm in Spilanthes acmella (L.) Murray and 0.4 to 2.1μm in Spilanthes calva DC. The relative length ranged from 2.3 to 12.1 μm in the species Spilanthes calva and 2.0 to 8.5 μm in the species Spilanthes acmella. The Karyotype formula were M16+m2+sm16+st2 in Spilanthes calva DC. and M22 +sm14 in Spilanthes acmella (L.) Murray cytologically obtained by using 2% aceto-orcein. The karyotype of the two species shows considerable variation exists in morphology of chromosome and structure. The variation in karyomorphology shows evolved nature of this species which play a great role in evolution.","PeriodicalId":13876,"journal":{"name":"International Journal of Applied Sciences and Biotechnology","volume":"134 1","pages":"422-426"},"PeriodicalIF":0.0,"publicationDate":"2020-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90417833","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Benefit Cost Analysis of Small Farm Machineries Used for Rice Cultivation in Nepal 尼泊尔用于水稻种植的小型农业机械的效益成本分析

International Journal of Applied Sciences and Biotechnology Pub Date : 2020-12-28 DOI: 10.3126/ijasbt.v8i4.31928

P. Acharya, P. Regmi, D. Gauchan, Dilli Bahadur, Gopal Bahadur

引用次数: 2

Repurposing Artemisia annua L. Flavonoids, Artemisinin and Its Derivatives as Potential Drugs Against Novel Coronavirus (SARS –nCoV) as Revealed by In-Silico Studies 利用黄花蒿类黄酮、青蒿素及其衍生物作为抗新型冠状病毒(SARS -nCoV)的潜在药物的硅研究

International Journal of Applied Sciences and Biotechnology Pub Date : 2020-12-28 DOI: 10.3126/ijasbt.v8i4.33667

K. Rai, Apoorva, Lakee Sharma, N. Pandey, R. Meena, S. Rai

引用次数: 5