International Journal of Antimicrobial Agents最新文献

{"title":"International Society of Antimicrobial Chemotherapy (ISAC) News and Information Page","authors":"","doi":"10.1016/j.ijantimicag.2025.107558","DOIUrl":"10.1016/j.ijantimicag.2025.107558","url":null,"abstract":"","PeriodicalId":13818,"journal":{"name":"International Journal of Antimicrobial Agents","volume":"66 3","pages":"Article 107558"},"PeriodicalIF":4.9,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144548778","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multi-omics reveals the synergistic mechanism of polymyxin B and diethyldithiocarbamate against Pseudomonas aeruginosa","authors":"Yanan Bai ,&nbsp;Pengqin Chen ,&nbsp;Wenhao Wu ,&nbsp;Qing Yang ,&nbsp;Tingting Qu ,&nbsp;Wuping Shuai ,&nbsp;Yongzhong Du ,&nbsp;Saiping Jiang","doi":"10.1016/j.ijantimicag.2025.107569","DOIUrl":"10.1016/j.ijantimicag.2025.107569","url":null,"abstract":"<div><h3>Objectives</h3><div>Resistance to polymyxin B (PMB) in carbapenem-resistant <em>Pseudomonas aeruginosa</em> (CRPA) has become a global issue. A promising approach to rescue PMB treatment failures is to repurpose non-antibiotics as adjuvants to enhance the efficacy of antibiotics against CRPA.</div></div><div><h3>Methods</h3><div>The in vitro effects of the PMB/DDC combination were assessed by microbroth dilution method, checkerboard microbroth dilution method, time-kill assay, biofilm formation inhibition assay, live/dead bacterial staining, and scanning electron microscopy. The in vivo efficacy of the PMB/DDC combination was evaluated in a PMB-resistant <em>P. aeruginosa</em> infection model. Comprehensive multi-omics analysis was performed to investigate the mechanism of the PMB/DDC combination.</div></div><div><h3>Results</h3><div>We identified that the combination of PMB and diethyldithiocarbamate (DDC) had a high synergistic effect against PMB-resistant <em>Pseudomonas aeruginosa</em> (<em>P. aeruginosa</em>) strains in vitro and significantly hindered the biofilm formation. The PMB/DDC combination was effective in treating PMB-resistant <em>P. aeruginosa</em> infection model in vivo, as evidenced by higher survival rates in five days and lower bacterial loads in lung tissue and liver. Comprehensive multi-omics analysis revealed that the resistance of <em>P. aeruginosa</em> P2550 to PMB was determined by chromosomally encoded rather than plasmid-mediated mobilized colistin resistance (MCR) clusters. The combination down-regulated the expression of the operons <em>arnBCADTEF</em> at 2 h, which regulate LPS modification, and the abundance of metabolites of amino sugars and nucleotide sugars related to Lipid A biosynthesis and Lipid A modification were significantly perturbed. The Gac/Rsm pathway, quorum sensing (QS) system, cAMP/Vfr signalling, and c-di-GMP signalling, which are pathways associated with biofilm formation, were significantly inhibited within the first 4 h. In addition, the PMB/DDC combination significantly disturbed metabolic pathways, including arginine biosynthesis, the tricarboxylic acid cycle (TCA), and nucleotide metabolism. Notably, PMB alone, DDC alone, and the combination disrupted membrane-associated glycerophospholipid metabolism.</div></div><div><h3>Conclusions</h3><div>The study highlights the potential of the PMB/DDC combination for treating PMB-resistant <em>P. aeruginosa</em> infections and elucidates the synergistic bactericidal mechanism of the combination through multi-omics methods.</div></div>","PeriodicalId":13818,"journal":{"name":"International Journal of Antimicrobial Agents","volume":"66 5","pages":"Article 107569"},"PeriodicalIF":4.6,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144567421","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Human serum lipids affect Staphylococcus aureus sensitivity to phage infection","authors":"Claudia Campobasso ,&nbsp;Elisa Fausti ,&nbsp;Daria Bottai ,&nbsp;Barbara Turchi ,&nbsp;Novella Cesta ,&nbsp;Laura Rindi ,&nbsp;Giulia Freer ,&nbsp;Arianna Tavanti ,&nbsp;Mariagrazia Di Luca","doi":"10.1016/j.ijantimicag.2025.107568","DOIUrl":"10.1016/j.ijantimicag.2025.107568","url":null,"abstract":"<div><h3>Objectives</h3><div>To explore phage therapy as an alternative for targeting multidrug-resistant bacterial strains, it is crucial to study phage-bacteria interaction under conditions that mimic <em>in vivo</em> environments. Recent studies have demonstrated that staphylococcal phage activity can be significantly hindered by human blood components, including plasma and serum. This study aimed to identify serum components responsible for phage inhibition and assess whether this effect occurred across multiple <em>Staphylococcus aureus</em> strains.</div></div><div><h3>Methods</h3><div>Phage Sb-1 activity against <em>S. aureus</em> ATCC 43300 was tested by pre-incubating or co-incubating bacteria and phages with 30% (v/v) heat-inactivated (56 °C and 80 °C) human, bovine, or foetal calf serum, IgG-depleted or delipidated serum, and BSA. Bacteria were incubated with serum before phage exposure, followed by washing with 0.1% Triton X-100. Additionally, growth kinetics of ten <em>S. aureus</em> strains incubated with or without Sb-1 were assessed over 24 hours in the presence of human serum.</div></div><div><h3>Results</h3><div>We found that adult human serum completely impairs phage infectivity due to interactions between serum components and bacterial cells rather than direct phage neutralisation. Albumin, IgG, and thermolabile components were demonstrated not to significantly contribute to the inhibitory effect, whereas lipids were identified as playing a key role. Furthermore, the sensitivity of different bacterial strains to phages was shown to be differentially affected by the presence of serum, as two of the strains tested remained susceptible to lysis despite serum exposure.</div></div><div><h3>Conclusions</h3><div>Taken together, our findings suggest that serum lipid fraction impacts phage infectivity in a strain-specific manner, highlighting the need for tailored approaches for phage therapy.</div></div>","PeriodicalId":13818,"journal":{"name":"International Journal of Antimicrobial Agents","volume":"66 4","pages":"Article 107568"},"PeriodicalIF":4.6,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144564766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Long-distance transmission of tmexCD1-toprJ1 positive Klebsiella pneumoniae between humans and food animals","authors":"Chao Liu ,&nbsp;Pengcheng Du ,&nbsp;Ping Yang ,&nbsp;Yunfei Tang ,&nbsp;Juan Yi ,&nbsp;Qiaojun Wang ,&nbsp;Chunjing Du ,&nbsp;Ming Lu ,&nbsp;Ruichao Li ,&nbsp;Ning Shen","doi":"10.1016/j.ijantimicag.2025.107566","DOIUrl":"10.1016/j.ijantimicag.2025.107566","url":null,"abstract":"<div><h3>Objectives</h3><div>The <em>tmexCD1-toprJ1</em> RND efflux pump gene cluster is predominantly identified among <em>Klebsiella pneumoniae</em> (<em>K. pneumoniae</em>) from humans and food animals. However, the mechanism of the long and rapid dissemination of such resistance determinant among strains from humans, food animals, and the environment is unclear.</div></div><div><h3>Methods</h3><div><em>K. pneumoniae</em> isolates were collected from two surveillance projects among patients and food animals. Whole-genome sequencing for phylogenetically close-related <em>tmexCD1-toprJ1</em>-positive and negative isolates, and phylogenic, plasmid stability, and fitness cost analyses were performed.</div></div><div><h3>Results</h3><div>Among 2592 clinical <em>K. pneumoniae</em> isolates and 243 <em>K. pneumoniae</em> isolates from food animals, the minimum inhibitory concentrations (MICs) of tigecycline (TGC) were 2 to 32 mg/L. The <em>tmexCD1-toprJ1</em>-positive isolates belonged to five sequence types (STs) and exhibited TGC resistance. By phylogenetic analyses, we have shown that <em>tmexCD1-toprJ1</em>-positive ST22 may have derived from <em>tmexCD1-toprJ1</em>-negative isolates in our hospital, whereas <em>tmexCD1-toprJ1</em>-positive ST726 showed a long-distance transmission among human and food animals. Different types of plasmids harbouring <em>tmexCD1-toprJ1</em> were identified, which have been disseminated among bacteria from humans, food animals, and the environment. Interestingly, low fitness costs of the <em>tmexCD1-torpJ1</em>-positive isolates compared to the phylogenetically close <em>tmexCD1-toprJ1</em>-negative isolates.</div></div><div><h3>Conclusions</h3><div>The long and rapid transmission of <em>tmexCD1-toprJ1</em> was predominantly caused by plasmids. In One Health, systematic surveillance and studies of isolates from different sources are urgently needed to understand the mechanism of resistance genes dissemination.</div></div>","PeriodicalId":13818,"journal":{"name":"International Journal of Antimicrobial Agents","volume":"66 5","pages":"Article 107566"},"PeriodicalIF":4.6,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144567420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Large scale laboratory evolution uncovers clinically relevant collateral antibiotic sensitivity","authors":"Farhan R. Chowdhury ,&nbsp;Veronica Banari ,&nbsp;Vlada Lesnic ,&nbsp;George G. Zhanel ,&nbsp;Brandon L. Findlay","doi":"10.1016/j.ijantimicag.2025.107564","DOIUrl":"10.1016/j.ijantimicag.2025.107564","url":null,"abstract":"<div><div>The increasing prevalence of antibiotic resistance is a critical challenge, necessitating the development of strategies to mitigate the evolution of resistance. Collateral sensitivity (CS)-based sequential therapies have been proposed to mitigate resistance evolution.</div><div>However, the evolutionary repeatability of CS across different experimental conditions and its clinical relevance remain underexplored, hindering its potential for translation into clinical practice. Here, we evolve 20–24 lineages of <em>E. coli</em> against tigecycline (TIG) and piperacillin (PIP), antibiotics suggested to produce CS, through three separate laboratory adaptive evolution (ALE) platforms to test for the robustness of CS interactions and the effect of the choice of ALE on CS evolution. We generate over 130 resistant mutants and 540 resistance and collateral sensitivity measurements to identify a CS relationship between TIG and polymyxin B (POL) that is highly repeatable across all the ALEs tested, suggesting that this CS interaction is preserved across different evolution microenvironments. We determine the mechanism of this novel CS by showing that cells resistant to TIG deactivate the Lon protease and overproduce negatively charged exopolysaccharides, which in turn attracts the polycationic POL and renders cells hypersensitive to the drug. We find that this CS relationship is present in a clinical dataset of over 750 uropathogenic MDR <em>E. coli</em> isolates, and show that the soft agar gradient evolution (SAGE) platform best predicts collateral effects (CS, neutrality or cross resistance) in this dataset. Our study provides a framework for identifying robust CS with clinical implications that can reduce the emergence of resistance to our existing antibiotics.</div></div>","PeriodicalId":13818,"journal":{"name":"International Journal of Antimicrobial Agents","volume":"66 4","pages":"Article 107564"},"PeriodicalIF":4.9,"publicationDate":"2025-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144564777","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lyophilised fecal microbiota transfer in capsules for recurrent Clostridioides difficile infection","authors":"Lourdes Daneri ,&nbsp;Alicia Urbano ,&nbsp;Rosa Escudero-Sánchez ,&nbsp;Ana Verónica Halperin ,&nbsp;Ana Moreno-Blanco ,&nbsp;María Dolores Corbacho ,&nbsp;Cecilia Suárez-Carantoña ,&nbsp;Concepción Rodríguez-Jiménez ,&nbsp;Sergio Serrano-Villar ,&nbsp;Rosa del Campo ,&nbsp;Javier Cobo","doi":"10.1016/j.ijantimicag.2025.107561","DOIUrl":"10.1016/j.ijantimicag.2025.107561","url":null,"abstract":"<div><h3>Background</h3><div>Recent guidelines recommend fecal microbiota transplantation (FMT) for patients who experience multiple episodes of <em>Clostridioides difficile</em> infection (CDI). The availability of lyophilised and encapsulated FMT in recent years has greatly improved patient comfort and convenience. While the effectiveness of FMT in oral capsules seems comparable to that achieved through other routes, further experience is needed, particularly in Europe, where there is currently limited published experience. The objective of this study was to present our experience with this therapeutic modality.</div></div><div><h3>Methods</h3><div>A retrospective cohort study on patients with recurrent CDI treated by lyophilised, encapsulated FMT. All patients were followed for a minimum of 12 weeks. The primary outcome was recurrence at three months.</div></div><div><h3>Results</h3><div>A total of 36 patients received 38 FMTs. The median age of the cohort was 78.5 years, with a median of four previous episodes. At the three-month follow-up, 27 of the 36 patients (75.0%) were free of CDI. One patient exhibited recurrence before the six-month mark. Two of the ten patients with FMT failure were successfully rescued with a second FMT. Of the nine patients who underwent rescue attempts, seven did not experience recurrence, resulting in a cure rate of 91.7% for the 36 patients. We did not detect severe adverse effects related to the FMT.</div></div><div><h3>Conclusion</h3><div>We confirm an acceptable effectiveness of lyophilised capsulated oral FMT. Interestingly, most patients with FMT failure can be cured with a new treatment, which need not necessarily be a new FMT.</div></div>","PeriodicalId":13818,"journal":{"name":"International Journal of Antimicrobial Agents","volume":"66 4","pages":"Article 107561"},"PeriodicalIF":4.6,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144560079","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Spanish nationwide survey of Pseudomonas aeruginosa cefiderocol antimicrobial susceptibility and resistance genomics","authors":"Miquel Àngel Sastre-Femenia ,&nbsp;Almudena Fernández-Muñoz ,&nbsp;María Antonia Gomis-Font ,&nbsp;Biel Taltavull ,&nbsp;Carla López-Causapé ,&nbsp;Jorge Arca-Suárez ,&nbsp;Luis Martínez-Martínez ,&nbsp;Rafael Cantón ,&nbsp;Nieves Larrosa ,&nbsp;Jesús Oteo-Iglesias ,&nbsp;Antonio Oliver","doi":"10.1016/j.ijantimicag.2025.107563","DOIUrl":"10.1016/j.ijantimicag.2025.107563","url":null,"abstract":"<div><h3>Objective</h3><div>Cefiderocol is a new siderophore-cephalosporin showing potent activity against <em>Pseudomonas aeruginosa</em>, including isolates showing extensively drug-resistant (XDR) or difficult-to-treat resistant (DTR) phenotypes. However, there is still a limited understanding of the potential resistance mechanisms. The objective of this study was to analyse the activity of cefiderocol in a nationwide Spanish survey, determine its stability against most relevant resistance mechanisms, and analyse potential drivers of resistance through whole-genome sequencing.</div></div><div><h3>Methods</h3><div>Cefiderocol MICs were determined by broth microdilution in cation-adjusted iron-depleted Müller-Hinton broth for 1735 isolates from 66 Spanish hospitals and compared with those of a panel of 13 antipseudomonal agents. All XDR/DTR strains and those resistant to ceftolozane/tazobactam, ceftazidime/avibactam, imipenem/relebactam or cefiderocol were subjected to whole-genome sequencing (NovaSeq, Illumina, San Diego, California, US) and bioinformatic analysis, including specific pipelines developed for the assessment of acquired resistance determinants, the mutational resistome and iron-uptake genes.</div></div><div><h3>Results</h3><div>Cefiderocol showed the highest percentage of susceptibility (99.7%) and conserved activity (&lt;5–10% resistance) against XDR/DTR strains and those producing carbapenemases. Only five resistant isolates were detected and the complex resistome included acquired β-lactamases or AmpC mutations along with mutations in iron-uptake systems and AmpC and/or efflux pump regulators. Novel drivers of resistance such as <em>piuE</em> were identified. Moreover, the production of acquired oxacillinases and mutations in <em>ampC, mexR</em> or <em>piuA</em> were statistically associated with increased MICs, whereas mutations in <em>gyrA, parC</em> or <em>pvdS</em> were associated with increased susceptibility.</div></div><div><h3>Conclusion</h3><div>While cefiderocol shows potent activity against <em>P. aeruginosa</em>, active surveillance at the phenotypic and genomic level for the emergence of resistant strains should be implemented.</div></div>","PeriodicalId":13818,"journal":{"name":"International Journal of Antimicrobial Agents","volume":"66 4","pages":"Article 107563"},"PeriodicalIF":4.6,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144560080","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficacy of a bacteriophage cocktail (Arash and Ariobarzanes) in the treatment of Salmonella typhimurium infection in rat model","authors":"Mohammad Hashem Yousefi ,&nbsp;Jeroen Wagemans ,&nbsp;Saeed Nazifi ,&nbsp;Fatemeh Namazi ,&nbsp;Seyed Shahram Shekarforoush ,&nbsp;Saeid Hosseinzadeh","doi":"10.1016/j.ijantimicag.2025.107560","DOIUrl":"10.1016/j.ijantimicag.2025.107560","url":null,"abstract":"<div><h3>Objective</h3><div>The present study exploits <em>Salmonella typhimurium</em>-specific bacteriophages for their application in the treatment of infections caused by <em>S. typhimurium</em> ATCC 14028 in male Sprague-Dawley rats.</div></div><div><h3>Methods</h3><div>A bacteriophage cocktail including two phages, Arash and Ariobarzanes, belonging to the genera <em>Arashvirus</em> and <em>Chivirus</em>, respectively, was administered to rats inoculated with <em>S. typhimurium</em>, and microbiological, haematological, pathological, and immunological parameters were examined.</div></div><div><h3>Results</h3><div>Bacteriophage treatment resulted in a decrease in <em>Salmonella</em> spp. shedding in faeces and their internal organs, as well as at the reduction of the levels of inflammatory cytokines, including tumour necrosis factor alpha and interleukin-8, on different days from d 0 to d 9 post-infection. However, oral gavage of this phage cocktail did not have a significant and visible effect on the improvement of pathological lesions in organs such as the liver and cecum, as well as on haematological and serum biochemical indices.</div></div><div><h3>Conclusions</h3><div>Therefore, a cocktail including phages Arash and Ariobarzanes can at least reduce the <em>S. typhimurium</em> counts in faeces, organs, and inflammatory mediators in rats with Salmonellosis infection.</div></div>","PeriodicalId":13818,"journal":{"name":"International Journal of Antimicrobial Agents","volume":"66 4","pages":"Article 107560"},"PeriodicalIF":4.9,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144527839","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rapid detection of cefepime-taniborbactam susceptibility/resistance in Enterobacterales","authors":"Christophe Le Terrier ,&nbsp;Laurent Poirel ,&nbsp;Auriane Kerbol ,&nbsp;Maxime Bouvier ,&nbsp;Patrice Nordmann","doi":"10.1016/j.ijantimicag.2025.107557","DOIUrl":"10.1016/j.ijantimicag.2025.107557","url":null,"abstract":"<div><h3>Objective</h3><div>Metallo-β-lactamase–producing Enterobacterales is widely distributed and confers resistance to all β-lactams, including carbapenems. Recently, a cefepime-taniborbactam combination has been developed that aims to provide a treatment option for infections caused by metallo-β-lactamase–producing bacteria. The objective of this study was to develop a rapid diagnostic test, namely the Rapid Cefepime-Taniborbactam NP test, for the early identification of cefepime-taniborbactam susceptibility or resistance in Enterobacterales.</div></div><div><h3>Methods</h3><div>The Rapid Cefepime-Taniborbactam NP test is based on the detection of glucose metabolism resulting from bacterial growth, detectable by a change in colour of a red phenol (from red to yellow) in the presence of cefepime-taniborbactam. A total of 103 Enterobacterales isolates, including 94 carbapenemase producers, were selected for evaluation of the performance of the Rapid Cefepime-Taniborbactam NP test. In the absence of available breakpoints, two provisional breakpoints were taken for the interpretation—one corresponding to the current Clinical and Laboratory Standards Institute cefepime resistance breakpoint (<em>R</em> ≥ 16 mg/L) and one following current literature on this combination (<em>R</em> ≥ 32 mg/L).</div></div><div><h3>Results</h3><div>The test demonstrated a sensitivity and specificity of 95% and 98%, respectively, following a resistance breakpoint of ≥16 mg/L. When considering a resistance breakpoint of ≥32 mg/L, the sensitivity and specificity were found to be 100% and 91%, respectively. All results were obtained within a 3 h incubation period at 35°C ± 2°C, representing a significant reduction in time compared with current antimicrobial susceptibility testing methods.</div></div><div><h3>Conclusions</h3><div>The results of this study demonstrate that the Rapid Cefepime-Taniborbactam NP test is a highly accurate and time-efficient technique for the detection of bacterial resistance.</div></div>","PeriodicalId":13818,"journal":{"name":"International Journal of Antimicrobial Agents","volume":"66 4","pages":"Article 107557"},"PeriodicalIF":4.9,"publicationDate":"2025-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144368846","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In reply to the letter to the editor regarding “Bayesian evaluation of phage therapy efficacy against multidrug-resistant Acinetobacter Baumannii”","authors":"Momna Arooj Malik ,&nbsp;Sobia Manzoor ,&nbsp;Javed Ashraf","doi":"10.1016/j.ijantimicag.2025.107555","DOIUrl":"10.1016/j.ijantimicag.2025.107555","url":null,"abstract":"","PeriodicalId":13818,"journal":{"name":"International Journal of Antimicrobial Agents","volume":"66 4","pages":"Article 107555"},"PeriodicalIF":4.9,"publicationDate":"2025-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144368844","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}