International Journal of Antimicrobial Agents最新文献

{"title":"Title Page & Editorial Board","authors":"","doi":"10.1016/S0924-8579(25)00086-X","DOIUrl":"10.1016/S0924-8579(25)00086-X","url":null,"abstract":"","PeriodicalId":13818,"journal":{"name":"International Journal of Antimicrobial Agents","volume":"66 1","pages":"Article 107529"},"PeriodicalIF":4.9,"publicationDate":"2025-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143905845","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"International Society of Antimicrobial Chemotherapy (ISAC) News and Information Page","authors":"","doi":"10.1016/j.ijantimicag.2025.107525","DOIUrl":"10.1016/j.ijantimicag.2025.107525","url":null,"abstract":"","PeriodicalId":13818,"journal":{"name":"International Journal of Antimicrobial Agents","volume":"66 1","pages":"Article 107525"},"PeriodicalIF":4.9,"publicationDate":"2025-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143877438","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Title Page & Editorial Board","authors":"","doi":"10.1016/S0924-8579(25)00073-1","DOIUrl":"10.1016/S0924-8579(25)00073-1","url":null,"abstract":"","PeriodicalId":13818,"journal":{"name":"International Journal of Antimicrobial Agents","volume":"65 6","pages":"Article 107516"},"PeriodicalIF":4.9,"publicationDate":"2025-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143825414","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"International Society of Antimicrobial Chemotherapy (ISAC) News and Information Page","authors":"","doi":"10.1016/j.ijantimicag.2025.107509","DOIUrl":"10.1016/j.ijantimicag.2025.107509","url":null,"abstract":"","PeriodicalId":13818,"journal":{"name":"International Journal of Antimicrobial Agents","volume":"65 6","pages":"Article 107509"},"PeriodicalIF":4.9,"publicationDate":"2025-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143806860","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Is use of antimicrobial growth promoters linked to antimicrobial resistance in food-producing animals? A systematic review.","authors":"Floriane Etienne, Thibaut Lurier, Javier Yugueros-Marcos, Ana Luisa Pereira Mateus","doi":"10.1016/j.ijantimicag.2025.107505","DOIUrl":"https://doi.org/10.1016/j.ijantimicag.2025.107505","url":null,"abstract":"<p><p>Antimicrobial resistance (AMR) poses a global threat to the health of humans, animals and plants, as well as the environment. In recent years, attention has increasingly focused on the role of antimicrobials as growth promoter (AGPs) in livestock. While the mechanism of action of AGPs is still poorly understood, mounting evidence suggests a link between AGP use and AMR. Consequently, several countries and regions have restricted/banned AGP use in livestock. However, such efforts encounter political, financial, social, and cultural challenges. This systematic review aims to investigate the impact of AGP use on AMR in food-producing animals and focused on the emergence of phenotypic resistance in Escherichia coli isolated from livestock exposed to AGPs. Overall, 7,000 studies were screened at title, abstract and full text; from these, 10 were deemed eligible for inclusion in this review. Among the 10 selected studies, seven noted significant increase of AMR associated with AGP use. Significantly increased resistance levels to Highest Priority Critically Important Antimicrobials for human health such as ceftiofur, cefotaxime, ciprofloxacin, and nalidixic acid were observed among other antimicrobials. However, the studies revealed high risk of bias underscoring the need for further research. This review provides a deeper understanding of the consequences of AGP use and occurrence of AMR. It highlights the need for implementing efficient surveillance systems and fostering research into suitable alternatives to AGPs. A shift to sustainable husbandry practices including responsible AMU while safeguarding animal health, productivity and farmers' livelihoods are essential for successful policy implementation.</p>","PeriodicalId":13818,"journal":{"name":"International Journal of Antimicrobial Agents","volume":" ","pages":"107505"},"PeriodicalIF":4.9,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143794385","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An accurate amplification-free CRISPR/Cas12a-based assay for GES β-lactamase detection","authors":"Daniel Pablo-Marcos ,&nbsp;Leticia Fernández-Diego ,&nbsp;Jorge Rodríguez-Grande ,&nbsp;Nuria Fraile-Valcárcel ,&nbsp;Concha Ortiz-Cartagena ,&nbsp;Olga Pacios ,&nbsp;Samuel García-García ,&nbsp;Sergio García-Fernández ,&nbsp;Lucía Blasco ,&nbsp;Alain Ocampo-Sosa ,&nbsp;Jorge Calvo-Montes ,&nbsp;María Tomás","doi":"10.1016/j.ijantimicag.2025.107506","DOIUrl":"10.1016/j.ijantimicag.2025.107506","url":null,"abstract":"<div><h3>Objective</h3><div>Guiana-Extended-Spectrum (GES) β-lactamases belong to the minor class A β-lactamases and are probably underdiagnosed due to a lack of specific diagnostic tests. There is therefore an urgent need to develop new molecular diagnostic tools that will be able to fill the gap in the detection of rare β-lactamases. Here, we propose an optimized, amplification-free CRISPR/Cas12a-based assay for the accurate detection of GES β-lactamases and we validate its application with clinical isolates <strong>(Graphic abstract)</strong>. Based on the results of examination of 79 standard collection, the proposed assay exhibited 100% sensitivity and specificity, as well as 100% positive and negative predictive values in less than 1.5 hours.</div></div><div><h3>Methods</h3><div>We optimized the CRISPR/Cas12a method by harnessing a multiplex crRNA strategy, a highly efficient DNA reporter (TTATT-5C) and the Murine RNase Inhibitor to prevent crRNA degradation.</div></div><div><h3>Results</h3><div>Our yielded limits of detection of 1 ng/µL and 3 ng/µL in <em>Enterobacterales</em> and <em>Pseudomonas aeruginosa</em>, respectively. The observed difference is due to the location of the <em>bla</em>_GES gene. The gene occurs in a chromosomal integron present only in one to three copies in <em>P. aeruginosa</em>, whereas it occurs in plasmids present in multiple copies in <em>Enterobacterales</em>.</div></div><div><h3>Conclusions</h3><div>The proposed method could be established as a routine diagnostic tool in clinical microbiology laboratories to fill the gap in availability of commercial diagnostic tests for GES β-lactamases.</div></div>","PeriodicalId":13818,"journal":{"name":"International Journal of Antimicrobial Agents","volume":"66 1","pages":"Article 107506"},"PeriodicalIF":4.9,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143788327","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Probiotic effects of Clostridium cellabutyricum against Pseudomonas aeruginosa infection in antibiotic-induced gut microbial dysbiosis mice model","authors":"Meng-Ling Wang ,&nbsp;Yuan-Jie Zhang ,&nbsp;Hong Xiao ,&nbsp;Xiao-Ling Lu,&nbsp;Li Chen,&nbsp;Zhi-Wen Ma,&nbsp;Anyi Chen,&nbsp;Qi Yin","doi":"10.1016/j.ijantimicag.2025.107503","DOIUrl":"10.1016/j.ijantimicag.2025.107503","url":null,"abstract":"<div><h3>Objective</h3><div>Gut microbiota dysbiosis induced by antibiotic use weakens its colonization resistance against opportunistic pathogens, increasing the risk of invasion and infection. While probiotics have the potential to restore the impaired gut microbial structure and prevent respiratory tract infections, the effectiveness of specific strains and the underlying mechanisms remain largely unexplored. In this study, the preventive effects of a novel butyrate-producing bacterium, <em>Clostridium cellabutyricum</em> YQ-FP-027^T against <em>Pseudomonas aeruginosa</em> infection after antibiotic exposure were investigated in antibiotic-pretreated mice model.</div></div><div><h3>Methods</h3><div>Phenotypic characterizations including the bacterial load in the lung, the assessment of gene expression of immune factors in lung tissue using qPCR, and detection of gut microbial composition using 16S rRNA sequencing were conducted. Pulmonary bacterial load and expression levels of immune factors of lung tissue, and gut microbial composition were evaluated.</div></div><div><h3>Results</h3><div>Our results demonstrated that YQ-FP-027^T ameliorated lung tissue integrity, significantly reduced pulmonary bacterial burden, and decreased the expression of interleukin-1β and TNF-α, while enhancing the expression of interleukin-10 and cathelicidin-related antimicrobial peptide. Furthermore, YQ-FP-027^T increased the abundance of <em>Lachnospiraceae</em> in the gut and reduced the abundance of opportunistic pathogens such as <em>Enterococcaceae</em> and <em>Helicobacteraceae</em>.</div></div><div><h3>Conclusions</h3><div>These results suggest YQ-FP-027^T exerts probiotic effects by restoring gut microbiota balance, enhancing intestinal barrier function, and positively influencing pulmonary immune responses through the gut-lung axis. This study reveals the preventive potential of YQ-FP-027^T against <em>P. aeruginosa</em> infection in the context of gut microbiota dysbiosis, offering a novel preventive strategy.</div></div>","PeriodicalId":13818,"journal":{"name":"International Journal of Antimicrobial Agents","volume":"66 1","pages":"Article 107503"},"PeriodicalIF":4.9,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143788330","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of Dientamoeba fragilis interhuman transmission by fecal microbiota transplantation.","authors":"Alicia Moreno-Sabater, Rachel Sintes, Sandrine Truong, Kimberly Lemoine, Océane Camou, Nathalie Kapel, Denis Magne, Anne-Christine Joly, Isabelle Quelven-Bertin, Laurent Alric, Christophe Hennequin, Harry Sokol","doi":"10.1016/j.ijantimicag.2025.107504","DOIUrl":"https://doi.org/10.1016/j.ijantimicag.2025.107504","url":null,"abstract":"<p><p>Fecal microbiota transplantation (FMT) for recurrent Clostridioides difficile infection (rCDI) requires careful selection of stool donors to avoid transmitting pathogens. Dientamoeba fragilis detection remains an exclusion criterion based on its uncertain pathogenicity. The aim of this study was to assess D. fragilis interhuman transmission by FMT and its impact on the clinical success of rCDI. A retrospective study was conducted in rCDI patients from the COSMIC cohort undergoing FMT to investigate the potential transfer of D. fragilis from donor to recipient. The impact of FMT involving D. fragilis was also evaluated on the clinical outcomes of rCDI and adverse effects. This protist was found to be present in 15 out of 86 healthy donors screened (18.7%) who voluntarily took part in an FMT program. Examination of D. fragilis presence in stool samples from 17 patients both before and after FMT with D. fragilis-positive donations revealed no evidence of interhuman transmission through this process. Analysis of clinical outcomes and adverse events in 124 rCDI patients who underwent FMT (with 45 receiving D. fragilis-positive donations) showed no significant differences in success rates between patients receiving positive or negative D. fragilis transplants, 95.5% and 93.6%, respectively. No significant variances were observed in other side effects analyzed. These findings underscore the safety of using fecal transplant from D. fragilis positive donors in the FMT process. D. fragilis should be removed from the donor screening, which will represent a major improvement in the donor selection process from financial and practical standpoints.</p>","PeriodicalId":13818,"journal":{"name":"International Journal of Antimicrobial Agents","volume":" ","pages":"107504"},"PeriodicalIF":4.9,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143788329","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Title Page & Editorial Board","authors":"","doi":"10.1016/S0924-8579(25)00058-5","DOIUrl":"10.1016/S0924-8579(25)00058-5","url":null,"abstract":"","PeriodicalId":13818,"journal":{"name":"International Journal of Antimicrobial Agents","volume":"65 5","pages":"Article 107501"},"PeriodicalIF":4.9,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143737835","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Profiling of omadacycline resistance in clinical MRSA: A nationwide genomic survey and in vitro evolutionary analysis","authors":"Yiyi Chen ,&nbsp;Yueqin Hong ,&nbsp;Xinru Wang ,&nbsp;Xin Cheng ,&nbsp;Feiteng Zhu ,&nbsp;Haiping Wang ,&nbsp;Zhengan Wang ,&nbsp;Shengnan Jiang ,&nbsp;Mengzhen Chen ,&nbsp;Hemu Zhuang ,&nbsp;Yeqiong Liu ,&nbsp;Yan Chen ,&nbsp;Lu Sun ,&nbsp;Yunsong Yu","doi":"10.1016/j.ijantimicag.2025.107499","DOIUrl":"10.1016/j.ijantimicag.2025.107499","url":null,"abstract":"<div><h3>Objective</h3><div>We aimed to evaluate the susceptibility of various clinical methicillin-resistant <em>Staphylococcus aureus</em> (MRSA) lineages to omadacycline and investigate the mechanisms underlying omadacycline resistance.</div></div><div><h3>Methods</h3><div>Omadacycline MICs for all MRSA isolates were determined via broth dilution. Representative clinical MRSA isolates of ST59, ST5 and ST9 were exposed to increasing concentrations of omadacycline. Mutants developing omadacycline resistance were isolated, sequenced, and compared using breseq. Molecular cloning was employed to elucidate the mechanisms of omadacycline resistance.</div></div><div><h3>Results</h3><div>Omadacycline MICs against MRSA ranged from 0.06 to 8 mg/L, with MIC₅₀ and MIC₉₀ values at 0.25 and 4 mg/L, respectively, and an overall resistance rate of 13%. All CC59 isolates were susceptible to omadacycline. Resistant isolates were mainly concentrated in HA-MRSA clones CC5. All 47 isolates with MICs ≥4 mg/L harbored <em>tet</em>(M) and the <em>rpsJ</em> K57M mutation. Cloning experiments demonstrated that both <em>tet</em>(M) and mutated <em>rpsJ</em> reduced susceptibility to omadacycline. The <em>rpsJ</em> gene was a common target in different MRSA lineages for decreased omadacycline susceptibility. Continuous exposure to omadacycline induced novel mutations in <em>rpsJ</em> (H56Y in ST9; H56R, K57M in ST59; and K57M, H56Y in ST5), which cloning experiments confirmed could variably reduce omadacycline susceptibility. Furthermore, mutated <em>mepA</em> also contributed to reduced omadacycline susceptibility.</div></div><div><h3>Conclusion</h3><div>Susceptibility to omadacycline varied among different MRSA lineages, while some CC5 isolates exhibiting the resistance phenotype. The <em>rpsJ</em> gene serves as a general target for the evolution of omadacycline resistance and plays an important role in the refinement of future tetracycline derivatives.</div></div>","PeriodicalId":13818,"journal":{"name":"International Journal of Antimicrobial Agents","volume":"66 1","pages":"Article 107499"},"PeriodicalIF":4.9,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143779703","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}