International Journal of Antimicrobial Agents最新文献

{"title":"Geographic variations in distributions of carbapenemase-encoding genes, susceptibilities, and minimum inhibitory concentrations of inpatient meropenem-resistant Enterobacterales to ceftazidime-avibactam, meropenem-vaborbactam, and aztreonam-avibactam across four global regions: 2020–2022 data from the Antimicrobial Testing Leadership and Surveillance","authors":"Shio-Shin Jean ,&nbsp;Chih-Cheng Lai ,&nbsp;Sung-Jung Ho ,&nbsp;I-Min Liu ,&nbsp;Po-Chuen Hsieh ,&nbsp;Po-Ren Hsueh","doi":"10.1016/j.ijantimicag.2025.107500","DOIUrl":"10.1016/j.ijantimicag.2025.107500","url":null,"abstract":"<div><h3>Purpose</h3><div>To evaluate the susceptibility profiles of regional inpatient meropenem-resistant (MEM-R) carbapenemase-producing Enterobacterales (CPE) isolates and their MIC values to ceftazidime-avibactam (CZA), meropenem-vaborbactam (MVB), and aztreonam-avibactam (ATM-AVI)</div></div><div><h3>Methods</h3><div>The 2020–2022 Antimicrobial Testing Leadership and Surveillance database were analyzed. Carbapenemase-encoding genes in CPE isolates were identified using multiplex PCR and Sanger sequencing. Susceptibility breakpoints for CZA and MVB recommended by CLSI 2024 and EUCAST 2025 against Enterobacterales were applied.</div></div><div><h3>Results</h3><div>A total of 2,318 CPE isolates (78.2% were <em>Klebsiella pneumoniae</em>) were tested globally. Notable diversity in carbapenemase-encoding gene distributions was observed among CPE isolates from Africa/the Middle East (10 countries; <em>n</em>=361), Asia (7 countries, excluding India and Pakistan; <em>n</em>=182), Europe (17 countries; <em>n</em>=1,002), and Latin America (10 countries; <em>n</em>=773). Metallo-β-lactamase-encoding genes, predominantly <em>bla</em>_NDM-1, were more frequently detected in CPE isolates from Africa/the Middle East (75.3%, except <em>bla</em>_NDM-5 in Kuwait) and Asia (67%, except <em>bla</em>_IMP-8 in Taiwan) compared to other regions. Among KPC variants, the KPC-2 enzyme was the predominant one in CPE isolates in Europe (43.4%, except for KPC-3 prevalent specifically in Italy) and in Latin America (62.1%). The susceptibility rates of all analyzed CPE isolates harboring only a single <em>bla</em>_KPC gene to CZA and MVB were 99.4% and 93.5%, respectively, based on the CLSI 2024 susceptibility breakpoints. The MIC_50/90 values of CPE isolates to ATM-AVI were 0.12/0.25 mg/L and 0.5/1 mg/L, respectively, regardless of collection region, dual carbapenemase production, or infection source.</div></div><div><h3>Conclusions</h3><div>The trends in resistance to novel antibiotics among contemporary CPE isolates need close monitoring.</div></div>","PeriodicalId":13818,"journal":{"name":"International Journal of Antimicrobial Agents","volume":"66 1","pages":"Article 107500"},"PeriodicalIF":4.9,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143772279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"International Society of Antimicrobial Chemotherapy (ISAC) News and Information Page","authors":"","doi":"10.1016/j.ijantimicag.2025.107497","DOIUrl":"10.1016/j.ijantimicag.2025.107497","url":null,"abstract":"","PeriodicalId":13818,"journal":{"name":"International Journal of Antimicrobial Agents","volume":"65 5","pages":"Article 107497"},"PeriodicalIF":4.9,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143696795","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the subcutaneous route for dalbavancin administration in a long-term suppressive therapy of orthopaedic device-related infection","authors":"Paula Pons-Oltra ,&nbsp;Eva Benavent ,&nbsp;Miriam Maireles ,&nbsp;Marta Ulldemolins ,&nbsp;Raul Rigo-Bonnin ,&nbsp;Oscar Murillo","doi":"10.1016/j.ijantimicag.2025.107498","DOIUrl":"10.1016/j.ijantimicag.2025.107498","url":null,"abstract":"","PeriodicalId":13818,"journal":{"name":"International Journal of Antimicrobial Agents","volume":"66 1","pages":"Article 107498"},"PeriodicalIF":4.9,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143729975","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Emergence of a clinical carbapenem resistant Escherichia coli co-harboring blandm-5 and mcr-1.1 on the same plasmid","authors":"Weichao Liao ,&nbsp;Jingjing Quan ,&nbsp;Lei Huang ,&nbsp;Mengyu Zhou ,&nbsp;Chen Zhang ,&nbsp;Lina Chen ,&nbsp;Huangdu Hu ,&nbsp;Junxin Zhou ,&nbsp;Xi Li ,&nbsp;Yan Jiang ,&nbsp;Yunsong Yu ,&nbsp;Dongdong Zhao ,&nbsp;Jiancang Zhou","doi":"10.1016/j.ijantimicag.2025.107495","DOIUrl":"10.1016/j.ijantimicag.2025.107495","url":null,"abstract":"<div><h3>Objectives</h3><div><em>Escherichia coli</em> is a major pathogen, and the emergence of carbapenem-resistant <em>E. coli</em> (CREC) significantly restricts clinical treatment options. Polymyxins are considered the last-line treatment for CREC infections; however, the occurrence of polymyxin-resistant CREC, particularly following reports of plasmid-mediated colistin resistance (<em>mcr</em>), poses an increasing challenge.</div></div><div><h3>Methods</h3><div>In this study, we identified a polymyxin-resistant CREC strain isolated from the rectal swab of a patient hospitalized in a hematology ward. Antimicrobial susceptibility testings, S1-PFGE, Southern blot analysis, Conjugation Experiment, whole genome sequencing (WGS) and bioinformatic analysis were used to characterize the strain.</div></div><div><h3>Results</h3><div>The strain showed resistance to all tested antimicrobial agents except tigecycline. By bioinformatic analysis, the strain was found to carry one copy of the <em>mcr-1.1</em> gene and two copies of <em>bla</em>_NDM-5 genes. One <em>bla</em>_NDM-5 and the <em>mcr-1.1</em> gene were co-located on a plasmid (pCDE2901_MCR-NDM), while the second <em>bla</em>_NDM-5 gene resided on another plasmid (pCDE2901_NDM). The <em>bla</em>_NDM-5 gene in pCDE2901_MCR-NDM was likely mobilized from pCDE2901_NDM via a transposon. The plasmid pCDE2901_NDM could successfully transferred from the donor strain CDE2901 to the recipient strain EC600, while the plasmid pCDE2901_MCR-NDM was unable to undergo horizontal transfer despite harboring mobile-associated genes.</div></div><div><h3>Conclusions</h3><div>Given the critical role of polymyxins in treating CREC infections, the co-transfer of polymyxin and carbapenem resistance may severely undermine the efficacy of clinical therapies. Strengthened surveillance and monitoring are imperative to curtail the spread of extensively drug-resistant (XDR) pathogens</div></div>","PeriodicalId":13818,"journal":{"name":"International Journal of Antimicrobial Agents","volume":"66 1","pages":"Article 107495"},"PeriodicalIF":4.9,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143729971","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tigecycline modulates LPS-induced inflammatory response in sepsis via NF-κB signalling pathways: Experimental insights into immune regulation","authors":"Lu Zhang ,&nbsp;Jun Li ,&nbsp;Meiqing Feng ,&nbsp;Xiaocheng Xu ,&nbsp;Weiyi Tang ,&nbsp;Yangyang Jiang ,&nbsp;Zhuye Xia ,&nbsp;Hongjie Liu ,&nbsp;Feiyang Shen ,&nbsp;Xiang Li ,&nbsp;Lijing Jiang","doi":"10.1016/j.ijantimicag.2025.107496","DOIUrl":"10.1016/j.ijantimicag.2025.107496","url":null,"abstract":"<div><h3>Background</h3><div>Sepsis is associated with high morbidity and high mortality and has strongly motivated intense studies into its mechanisms. Antibiotics, aimed to eradicate bacteria, have some impact on the immune system due to anti-inflammatory properties. Tigecycline, <strong>an antibiotic of the glycylcycline class</strong>, is commonly used for severe infections.</div></div><div><h3>Purpose</h3><div>This study aimed to investigate tigecycline's mechanism on the inflammatory response of sepsis to find new targets for sepsis treatment. The objective included (i) to observe the changes in inflammatory factors in LPS (lipopolysaccharide) induced septic mice after tigecycline administration, (ii) to detect the effect of tigecycline on macrophages NF-κB (nuclear factor kappa B) signalling.</div></div><div><h3>Methods</h3><div>For LPS-induced sepsis in mice and intervention with tigecycline, mice were first injected with tigecycline (6.5 mg/kg) via tail vein followed by LPS (15 mg/kg). Luminex analysis was performed on 16 mediators. NF-κB signalling pathway antibody chip detected the expression of target sites in macrophages of the LPS group and tigecycline + LPS group.</div></div><div><h3>Results</h3><div>Tigecycline has inhibitory effects on LPS-induced inflammatory response in septic mice, decreasing the concentrations of IL (interleukin)-6, IL-27, TNF-α (tumour necrosis factor-α), TNF RII, IFN-γ (interferon-gamma), CCL5/RANTES (C<img>C Motif Chemokine Ligand) while increasing IL-6Rα, IL-10, and TWEAK (TNF-related weak inducer of apoptosis). Tigecycline downregulated phosphorylation levels of key sites JNK (c-Jun N-terminal kinase)1/2/3, p-p65 (s468) and p-p105/p50 (s907) in NF-κB signalling.</div></div><div><h3>Conclusions</h3><div>Tigecycline may inhibit the excessive immune response induced by LPS in sepsis, which may cause a potential protective effect on the host through immune regulation.</div></div>","PeriodicalId":13818,"journal":{"name":"International Journal of Antimicrobial Agents","volume":"66 1","pages":"Article 107496"},"PeriodicalIF":4.9,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143729978","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unravelling AMR dynamics in the rumenofaecobiome: Insights, challenges and implications for One Health","authors":"Ziming Wu ,&nbsp;Mustasim Famous ,&nbsp;Theano Stoikidou ,&nbsp;Freya E.S. Bowden ,&nbsp;Gama Dominic ,&nbsp;Sharon A. Huws ,&nbsp;Fernanda Godoy-Santos ,&nbsp;Linda B. Oyama","doi":"10.1016/j.ijantimicag.2025.107494","DOIUrl":"10.1016/j.ijantimicag.2025.107494","url":null,"abstract":"<div><div>Antimicrobial resistance (AMR) is a critical global threat to human, animal and environmental health, exacerbated by horizontal gene transfer (HGT) via mobile genetic elements. This poses significant challenges that have a negative impact on the sustainability of the One Health approach, hindering its long-term viability and effectiveness in addressing the interconnectedness of global health. Recent studies on livestock animals, specifically ruminants, indicate that culturable ruminal bacteria harbour AMR genes with the potential for HGT. However, these studies have focused predominantly on using the faecobiome as a proxy to the rumen microbiome or using easily isolated and culturable bacteria, overlooking the unculturable population. These unculturable microbial groups could have a profound influence on the rumen resistome and AMR dynamics within livestock ecosystems, potentially holding critical insights for advanced understanding of AMR in One Health. In order to address this gap, this review of current research on the burden of AMR in livestock was undertaken, and it is proposed that combined study of the rumen microbiome and faecobiome, termed the ‘rumenofaecobiome’, should be performed to enhance understanding of the risks of AMR in ruminant livestock. This review discusses the complexities of the rumen microbiome and the risks of AMR transmission in this microbiome in a One Health context. AMR transmission dynamics and methodologies for assessing the risks of AMR in livestock are summarized, and future considerations for researching the impact of AMR in the rumen microbiome and the implications within the One Health framework are discussed.</div></div>","PeriodicalId":13818,"journal":{"name":"International Journal of Antimicrobial Agents","volume":"66 1","pages":"Article 107494"},"PeriodicalIF":4.9,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143691995","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Host immunity and intracellular bacteria evasion mechanisms: Enhancing host-directed therapies with drug delivery systems","authors":"Jiayang Cai ,&nbsp;Han Zhou ,&nbsp;Mingwei Liu,&nbsp;Dingjian Zhang,&nbsp;Jingxuan Lv,&nbsp;Haokun Xue,&nbsp;Houcheng Zhou,&nbsp;Wenli Zhang","doi":"10.1016/j.ijantimicag.2025.107492","DOIUrl":"10.1016/j.ijantimicag.2025.107492","url":null,"abstract":"<div><div>Host-directed therapies (HDTs) have been investigated as a potential solution to combat intracellular and drug-resistant bacteria. HDTs stem from extensive research on the intricate interactions between the host and intracellular bacteria, leading to a treatment approach that relies on immunoregulation. To improve the bioavailability and safety of HDTs, researchers have utilized diverse drug delivery systems (DDS) to encapsulate and transport therapeutic agents to target cells. In this review, we first introduce the three mechanisms of bactericidal action and intracellular bacterial evasion: autophagy, reactive oxygen species (ROS), and inflammatory cytokines, with a particular focus on autophagy. Special attention is given to the detailed mechanism of xenophagy in clearing intracellular bacteria, a crucial selective autophagy process that specifically targets and degrades intracellular pathogens. Following this, we present the application of DDS to modulate these regulatory methods for intracellular bacteria elimination. By integrating insights from immunology and nanomedicine, this review highlights the emerging role of DDS in advancing HDTs for intracellular bacterial infections and paving the way for innovative therapeutic interventions.</div></div>","PeriodicalId":13818,"journal":{"name":"International Journal of Antimicrobial Agents","volume":"65 6","pages":"Article 107492"},"PeriodicalIF":4.9,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143663381","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phylogenetic insights of a human-derived optrA-positive Staphylococcus aureus isolated from a person with chronic diarrhea in China","authors":"Aoxiao Chen ,&nbsp;Hanyu Wang ,&nbsp;Zelin Yan ,&nbsp;Ning Dong ,&nbsp;Rong Zhang ,&nbsp;Yonglu Huang ,&nbsp;Hongwei Zhou","doi":"10.1016/j.ijantimicag.2025.107493","DOIUrl":"10.1016/j.ijantimicag.2025.107493","url":null,"abstract":"","PeriodicalId":13818,"journal":{"name":"International Journal of Antimicrobial Agents","volume":"65 6","pages":"Article 107493"},"PeriodicalIF":4.9,"publicationDate":"2025-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143648630","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the protective role of recombinant type iii interferons in respiratory infections: Insights from an Mx1-deficient mouse model","authors":"A. Lozhkov ,&nbsp;O. Dobrovolskaya ,&nbsp;E. Romanovskaya-Romanko ,&nbsp;S. Shishlyannikov ,&nbsp;E. Elpaeva ,&nbsp;A. Garshinina ,&nbsp;Y. Zabrodskaya ,&nbsp;A. Shaldzhyan ,&nbsp;V. Egorov ,&nbsp;A. Vasin","doi":"10.1016/j.ijantimicag.2025.107491","DOIUrl":"10.1016/j.ijantimicag.2025.107491","url":null,"abstract":"<div><h3>Background</h3><div>Type III interferons (IFNs) are regarded as safe and effective preventive agents for viral infections of the respiratory tract. The effectiveness of the preventive use of type III IFNs in patients with polymorphisms in critical antiviral ISGs is particularly relevant. We utilized Balb/c mice deficient in the <em>Mx1</em> gene as model organisms.</div></div><div><h3>Methods</h3><div>Recombinant IFNs were administered intranasally one day before infection with the mouse-adapted pandemic strain of influenza A virus (IAV), A/California/07/09 (H1N1pdm09). In the model of mixed infection (sequential infection with IAV at a sublethal dose and <em>Staphylococcus aureus</em>), a bacterial suspension was administered intranasally a day after IAV infection. Influenza nucleoprotein level and bacterial load were assessed in the lungs on the third day post-infection.</div></div><div><h3>Results</h3><div>Type III IFNs demonstrated a protective effect against infection with IAV. A single intranasal administration of IFN-λ protected mice from weight loss, reduced the level of viral nucleoprotein in lung homogenates, and significant differences in survival curves were also observed. In the case of mixed infection, a single intranasal administration of IFN-λ was associated with a decrease in bacterial load in the lungs, and minimal weight loss was observed in the mice.</div></div><div><h3>Conclusion</h3><div>The study demonstrated that IFN-λ can have a protective effect in <em>Mx1</em>-deficient Balb/c mice. These data support the universality of type III IFN use, suggesting that these preventive agents can be effective even in patients with a suboptimal genetic background.</div></div>","PeriodicalId":13818,"journal":{"name":"International Journal of Antimicrobial Agents","volume":"66 1","pages":"Article 107491"},"PeriodicalIF":4.9,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143624450","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparing the Knowledge, Attitudes, and Practices of Antibiotic Use and Antimicrobial Resistance for Cats and/or Dogs amongst Pet Owners in Singapore: A Cross-sectional Study.","authors":"Seema Aithal, Gabriel Toh, Huiling Guo, Zoe Jane-Lara Hildon, Teo Boon Han, Timothy Chua, Angela Chow","doi":"10.1016/j.ijantimicag.2025.107489","DOIUrl":"https://doi.org/10.1016/j.ijantimicag.2025.107489","url":null,"abstract":"<p><p>Antibiotic use in pets can contribute to antimicrobial resistance (AMR) in both humans and pets. There is a paucity of data on pet owners' knowledge, attitudes, and practices regarding antibiotic use in pets in Asia, despite increasing pet ownership. Therefore, we conducted a study to compare the knowledge, attitudes and practices of antibiotic use and AMR for pets amongst cat and/or dog owners in Singapore. A cross-sectional survey among 1080 pet owners from 16 veterinary clinics between March 2023 and December 2023 evaluated pet owners' knowledge of antibiotic use, understanding of AMR, and antibiotic practices for their pets. Majority of pet owners were female (63.9%), higher educated (86.9%), owned dogs only (62.2%) and currently working (76.4%). Nearly half (46.3%) of all pet owners had poor knowledge of antibiotic use for pets, with cat owners demonstrating poorer knowledge than dog owners (55.2% vs 43.3%, p<0.001). While only one-third (33.9%) of all pet owners used antibiotics inappropriately, two-thirds (67.1%) demonstrated poor AMR knowledge. Cat owners preferred administering antibiotics in the form of liquids, whilst dog owners in the form of tablets. Pet owners play an important role in antibiotic use in pets. Addressing pet owners' poor knowledge of appropriate antibiotic use and AMR for pets is imperative, to strengthen antimicrobial stewardship efforts via a One Health approach.</p>","PeriodicalId":13818,"journal":{"name":"International Journal of Antimicrobial Agents","volume":" ","pages":"107489"},"PeriodicalIF":4.9,"publicationDate":"2025-03-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143604797","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}