International archives of allergy and applied immunology最新文献_第6页

In vitro development and functions of human mast cells. 人肥大细胞的体外发育与功能。

International archives of allergy and applied immunology Pub Date : 1991-01-01 DOI: 10.1159/000235341

T Ishizaka, T Furitsu, N Inagaki

{"title":"In vitro development and functions of human mast cells.","authors":"T Ishizaka, T Furitsu, N Inagaki","doi":"10.1159/000235341","DOIUrl":"https://doi.org/10.1159/000235341","url":null,"abstract":"A long-term co-culture of mononuclear cells of human umbilical cord blood with mouse embryo-derived 3T3 fibroblasts resulted in the development of human mast cells. These mast cells are morphologically and functionally mature cells, containing 1.4-2.8 micrograms histamine per 10(6) cells and bear approximately 10(5) Fc epsilon RI per cell. The mast cells sensitized with human IgE released histamine upon challenge with anti-IgE. Electron-microscopic analysis of the cells showed that these cells were mature human mast cells, and clearly different from basophilic granulocytes. Most of the mast cells contained some granules with regular crystalline arrays and both tryptase and chymase, resembling human skin mast cells. When mononuclear cells of cord blood were seeded in a millicell insert which was placed on 3T3 fibroblasts monolayer, the number of mast cells developed in the millicell inserts was comparable to those developed in the co-culture of the same cord blood cells with 3T3 fibroblasts. Recent observations that mast cells developed in the presence of concentrated culture supernatants of 3T3 fibroblasts without fibroblasts feeder layers, confirmed that soluble factors released from 3T3 fibroblasts are essential and sufficient for the differentiation of human mast cell progenitors in vitro. Analysis of functional characteristics of cultured mast cells revealed that they respond to anti-IgE, Ca2+ ionophore A23187 and substance P for histamine release, but failed to respond to compound 48/80 and FMLP. Upon anti-IgE challenge, sensitized mast cells generated approximately 80 ng PGD2 per 10(6) cells, and approximately 50 ng of LTC4 per 10(6) cells but no detectable generation of LTB4.(ABSTRACT TRUNCATED AT 250 WORDS)","PeriodicalId":13810,"journal":{"name":"International archives of allergy and applied immunology","volume":"94 1-4","pages":"116-21"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000235341","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13096704","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10

Histamine stimulation of the nasal mucosa does not induce prostaglandin or leukotriene generation or induce methacholine hyperresponsiveness. 组胺刺激鼻黏膜不诱导前列腺素或白三烯的生成或诱导乙酰胆碱的高反应性。

International archives of allergy and applied immunology Pub Date : 1991-01-01 DOI: 10.1159/000235420

A M Majchel, D Proud, W C Hubbard, R M Naclerio

{"title":"Histamine stimulation of the nasal mucosa does not induce prostaglandin or leukotriene generation or induce methacholine hyperresponsiveness.","authors":"A M Majchel, D Proud, W C Hubbard, R M Naclerio","doi":"10.1159/000235420","DOIUrl":"https://doi.org/10.1159/000235420","url":null,"abstract":"To further define the role of histamine in the nasal mucosa, we studied the possible effect of histamine provocation on the generation of prostanoids and leukotrienes, and on the induction of hyperresponsiveness to methacholine. In separate experiments, we performed nasal challenges with histamine and measured by gas chromatography negative ion mass spectrometry and by radioimmunoassay after high-performance liquid chromatography the levels of prostanoids and leukotrienes, respectively, in recovered nasal lavages 10 min after challenge. Hyperresponsiveness to methacholine was tested in both nostrils 24 h after unilateral provocation with histamine. Our data suggest that histamine induced an immediate symptomatic response, but neither led to the generation of prostaglandins or leukotrienes nor induced hyperresponsiveness to methacholine. These results differ from those achieved after antigen stimulation and emphasize the importance of mediators in addition to histamine in the allergic reaction.","PeriodicalId":13810,"journal":{"name":"International archives of allergy and applied immunology","volume":"95 2-3","pages":"149-55"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000235420","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13096867","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Analysis of 'clean' air samples by scanning and transmission electron microscopy and X-ray probe micro-analysis. 通过扫描和透射电子显微镜以及x射线探针显微分析分析“清洁”空气样本。

International archives of allergy and applied immunology Pub Date : 1991-01-01 DOI: 10.1159/000235404

F Chavarria, P Heap, F Carswell

引用次数: 0

Ragweed-specific IgA in nasal lavage fluid of ragweed-sensitive allergic rhinitis patients: increase during the pollen season. 豚草敏感变应性鼻炎患者鼻灌洗液中豚草特异性IgA在花粉季节升高。

International archives of allergy and applied immunology Pub Date : 1991-01-01 DOI: 10.1159/000235382

C E Reed, M Bubak, S Dunnette, J Blomgren, M Pfenning, P Wentz-Murtha, N Wallen, M Keating, G J Gleich

引用次数: 34

Faecally derived hydrolytic enzymes from Dermatophagoides pteronyssinus: physicochemical characterisation of potential allergens. 从翼状棘球绦虫粪便中提取的水解酶:潜在过敏原的理化特征。

International archives of allergy and applied immunology Pub Date : 1991-01-01 DOI: 10.1159/000235437

G A Stewart, F R Lake, P J Thompson

{"title":"Faecally derived hydrolytic enzymes from Dermatophagoides pteronyssinus: physicochemical characterisation of potential allergens.","authors":"G A Stewart, F R Lake, P J Thompson","doi":"10.1159/000235437","DOIUrl":"https://doi.org/10.1159/000235437","url":null,"abstract":"The previous findings that the group I and III mite allergens, and amylase present in mite faeces are hydrolytic enzymes has prompted a study to determine whether this material contains other enzymes which could be allergenic. Thus, spent growth medium devoid of whole Dermatophagoides pteronyssinus mites was shown to contain glucoamylase, lipase and lysozyme in addition to the cysteine protease, serine protease and amylase activities associated with the above allergens, respectively. All of these enzymes are probably associated with mite digestive processes. They were rapidly solubilised, heterogeneous with regard to charge (pI in the range 4-8) and demonstrated maximum biochemical activity in the neutral pH range. Three serine proteases were detected and comprised a chymotrypsin-like, a trypsin-like and an unclassified enzyme with pI of 4.1 and 5.3, 8.5 and 7.1, respectively. Only one cysteine protease was observed, which paralleled immunochemically identified Der p I in a variety of assays. It was shown to cleave at lysyl residues and could be inhibited by the specific cysteine protease inhibitor, E-64. The remaining serine proteases, glucoamylase, lipase and lysozyme represent potential allergens.","PeriodicalId":13810,"journal":{"name":"International archives of allergy and applied immunology","volume":"95 2-3","pages":"248-56"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000235437","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12882560","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 77

Extracellular ATP stimulates interleukin-dependent cultured mast cells and eosinophils through calcium mobilization. 细胞外ATP通过钙动员刺激白细胞介素依赖性培养肥大细胞和嗜酸性粒细胞。

International archives of allergy and applied immunology Pub Date : 1991-01-01 DOI: 10.1159/000235327

H Saito, M Ebisawa, D C Reason, K Ohno, K Kurihara, N Sakaguchi, A Ohgimi, E Saito, A Akasawa, K Akimoto

{"title":"Extracellular ATP stimulates interleukin-dependent cultured mast cells and eosinophils through calcium mobilization.","authors":"H Saito, M Ebisawa, D C Reason, K Ohno, K Kurihara, N Sakaguchi, A Ohgimi, E Saito, A Akasawa, K Akimoto","doi":"10.1159/000235327","DOIUrl":"https://doi.org/10.1159/000235327","url":null,"abstract":"We examined the effect of ATP and related nucleotides on the changes in intracellular calcium ([Ca2+]i) in murine bone marrow-derived mast cells (BMMC) and human cord blood-derived eosinophils (EO) cultured in the presence of interleukins. ATP, ADP and AMP released a substantial amount of histamine and leukotriene C4 from BMMC, and EO showed locomotive activity in response to ATP, ADP and GTP. These reactions were accompanied with an increase in [Ca2+]i in BMMC and in EO. The rise in [Ca2+]i in BMMC induced by ATP or antigen at optimal concentrations was inclined to be persisting. On the other hand, these nucleotides induced a rapid and transient rise in [Ca2+]i in EO. Purified human peripheral EO also exhibited locomotive activity and an increase in [Ca2+]i in response to ATP. These results indicate that extracellular ATP activates interleukin-dependent cultured mast cells and EO through Ca2+ mobilization, and suggest that ATP, which is known to be released from activated platelets or autonomic nerves, may stimulate in vivo counterparts of these cultured inflammatory cells.","PeriodicalId":13810,"journal":{"name":"International archives of allergy and applied immunology","volume":"94 1-4","pages":"68-70"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000235327","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12882596","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 26

Antiasthmatic effects of Picrorhiza kurroa: androsin prevents allergen- and PAF-induced bronchial obstruction in guinea pigs. 黑纹小弧菌的平喘作用:雄激素可预防豚鼠变应原和paf诱导的支气管阻塞。

International archives of allergy and applied immunology Pub Date : 1991-01-01 DOI: 10.1159/000235416

W Dorsch, H Stuppner, H Wagner, M Gropp, S Demoulin, J Ring

引用次数: 78

Effect of nitric oxide generators on ischemia-reperfusion injury and histamine release in isolated perfused guinea pig heart. 一氧化氮发生器对豚鼠离体心脏缺血再灌注损伤及组胺释放的影响。

International archives of allergy and applied immunology Pub Date : 1991-01-01 DOI: 10.1159/000235376

E Masini, F Gambassi, S Bianchi, L Mugnai, M Lupini, A Pistelli, P F Mannaioni

引用次数: 4

Early development of mast cells. 肥大细胞的早期发育。

International archives of allergy and applied immunology Pub Date : 1991-01-01 DOI: 10.1159/000235339

M Rottem, A S Kirshenbaum, D D Metcalfe

引用次数: 14

Mucosal nerves in endobronchial biopsies in asthma and non-asthma. 哮喘和非哮喘患者支气管内活检中的粘膜神经。

International archives of allergy and applied immunology Pub Date : 1991-01-01 DOI: 10.1159/000235396

P H Howarth, R Djukanovic, J W Wilson, S T Holgate, D R Springall, J M Polak

引用次数: 63