International dental journal最新文献

{"title":"The Accuracy and Trueness of Intraoral Scanners in Neonates With Cleft Lip and Palate: A Comparison of 3D Digital Models","authors":"Piyapat Chatborirak ,&nbsp;Krisadi Phannarus ,&nbsp;Pimsiri Kanpittaya ,&nbsp;Pintu-on Chantarawaratit","doi":"10.1016/j.identj.2025.04.014","DOIUrl":"10.1016/j.identj.2025.04.014","url":null,"abstract":"<div><h3>Introduction and aims</h3><div>Intraoral scanners have recently emerged as a safer and more convenient alternative to traditional impression materials for capturing alveolar morphology in patients with cleft anomalies. This study aimed to assess the accuracy of 3D digital models generated by different intraoral scanners in cleft neonates. Specifically, it focused on evaluating the trueness and precision of these scanners in producing models of unilateral complete cleft lip and palate.</div></div><div><h3>Methods</h3><div>Forty plaster models of unilateral cleft lip and palate from neonates were analyzed. Inter-segment distances, consisting of 37 anterior and 23 posterior parameters, were measured manually with a digital vernier caliper as the reference. The models were scanned using 5 intraoral scanners: 3Shape TRIOS 4, iTero Element 5D, Medit i700, PANDA P2, and Straumann Virtuo Vivo. The differences between tested values (from 3D scans) and reference values were expressed as error per millimeter. Precision was evaluated by performing repeated scans and measurements on 10% of the models. Statistical analyses included 1-sample t-test, Wilcoxon test, and Friedman test, with a significance level of 0.05. Results were further validated through 3D superimposition using Geomagic Control X software.</div></div><div><h3>Results</h3><div>Observer reliability was excellent, with an ICC value of 1.000. Significant differences in trueness were found for the Medit i700 scanner, with higher errors for posterior inter-segment distances (<em>P = .</em>021) and anterior distances when compared to other scanners (<em>P = .</em>001 to .013). Precision showed excellent agreement across all scanners (ICC = 0.999 to 1.000).</div></div><div><h3>Conclusion</h3><div>While all scanners met clinical acceptability standards, the Medit i700 demonstrated lower trueness in certain parameters.</div></div><div><h3>Clinical Relevance</h3><div>This study highlights the importance of scanner selection for accurate digital model generation for cleft neonates.</div></div>","PeriodicalId":13785,"journal":{"name":"International dental journal","volume":"75 4","pages":"Article 100834"},"PeriodicalIF":3.2,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144125396","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Artesunate Promotes Bone Remodeling Through TRAF6-Mediated NF-κB Signaling Under Orthodontic Stress in Diabetic Rats","authors":"Yuxiang Zhan ,&nbsp;Siqin Zhang ,&nbsp;Minjie Yang ,&nbsp;Yuchen Wang ,&nbsp;Jiaquan Li ,&nbsp;Xiaolin Nong","doi":"10.1016/j.identj.2025.04.011","DOIUrl":"10.1016/j.identj.2025.04.011","url":null,"abstract":"<div><h3>Objectives</h3><div>To determine the effects of artesunate (ART) on bone remodeling <em>in vivo</em> under orthodontic stress in diabetic rats and explore the underlying mechanisms <em>in vitro</em>.</div></div><div><h3>Materials and methods</h3><div>A rat model of type 2 diabetes mellitus with orthodontic tooth movement was established. The rats received ART and/or metformin (Met) orally. The effects of ART and Met on periodontium changes were evaluated using tartrate-resistant acid phosphatase and immunohistochemical staining. Molecular docking analyses were employed to investigate the mechanisms of ART action. <em>In vitro,</em> the effects of ART on osteogenic and osteoclastic activity were explored by examining TRAF6 and NF-κB expression under hyperglycemic and static pressure conditions via immunofluorescence and Western blotting.</div></div><div><h3>Results</h3><div>ART enhanced bone metabolism despite hyperglycemia, though mechanical stress still induced bone resorption. Treatment with ART alone or in combination with Met promoted osteogenesis. TRAF6, NF-κB and the OPG/RANKL/RANK signaling pathways have been identified as key mediators of these effects. The expression of the osteogenesis-associated factor OPG increased after ART and Met treatment, while that of TRAF6 and the osteoclast-associated factors RANKL and NF-κB decreased.</div></div><div><h3>Conclusions</h3><div>Increased bone resorption and decreased bone formation are characteristics of type 2 diabetes, impacting orthodontic tooth movement. ART administration alone promotes bone remodeling under static pressure and hyperglycemic conditions. These effects are mediated by lowering blood sugar levels, inhibiting osteoclast function, and improving osteogenesis through mechanisms closely associated with the OPG/TRAF6/NF-κB signaling pathway.</div></div>","PeriodicalId":13785,"journal":{"name":"International dental journal","volume":"75 4","pages":"Article 100831"},"PeriodicalIF":3.2,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144090276","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In Vitro Comparison of the Performance of Hydrophilic and Conventional Hydrophobic Resin-Based Fissure Sealants","authors":"Tahsin Hossain Anika ,&nbsp;Choltacha Harnirattisai ,&nbsp;Siriruk Nakornchai ,&nbsp;Varangkanar Jirarattanasopha","doi":"10.1016/j.identj.2025.04.005","DOIUrl":"10.1016/j.identj.2025.04.005","url":null,"abstract":"<div><h3>Background</h3><div>Hydrophilic sealants were developed to overcome hydrophobic sealant moisture sensitivity; however, there is still a limited understanding of their performance. This study aimed to compare microshear bond strength, penetration depth, and microleakage of hydrophilic UltraSeal XT hydro and hydrophobic Clinpro resin-based sealants placed in various surface conditions.</div></div><div><h3>Methods</h3><div>Seventy-two enamel slices and 60 molars were randomly assigned into two groups: Group 1 UltraSeal XT hydro and Group 2 Clinpro, which were further subdivided into three groups based on the enamel surface conditions (dry, slightly moist, and saliva-contaminated). After applying sealant and undergoing 5000 thermocycling cycles, microshear bond strength was tested. Additionally, penetration depth and microleakage were evaluated under a light microscope after staining with 50% silver nitrate solution.</div></div><div><h3>Results</h3><div>Group 1 demonstrated significantly higher microshear bond strength than Group 2 across all experimental conditions (<em>P</em> &lt; .001). Both sealants showed the highest microshear bond strengths under dry conditions, followed by slightly moist and saliva-contaminated conditions (<em>P</em> &lt; .001). The penetration depth between the two sealant types was comparable within each surface condition. However, both sealants showed significantly deeper penetration on dry enamel surfaces compared to slightly moist and saliva-contaminated surfaces (<em>P</em> &lt; .001). Similarly, both sealants exhibited significantly less microleakage on dry enamel surfaces compared to slightly moist and saliva-contaminated surfaces (<em>P</em> &lt; .001). While the mean microleakage value was similar between the two sealants under dry and saliva-contaminated conditions, Group 1 showed significantly lower microleakage than Group 2 under moist conditions (<em>P</em> &lt; .001).</div></div><div><h3>Conclusion</h3><div>The hydrophilic UltraSeal XT hydro sealant exhibited superior bond strength compared to the hydrophobic Clinpro sealant across all surface conditions. On slightly moist surfaces, UltraSeal XT hydro showed significantly lower microleakage than Clinpro. Both sealants exhibited superior sealing and retentive ability on dry enamel surface<strong>s</strong> compared to the other surfaces.</div></div>","PeriodicalId":13785,"journal":{"name":"International dental journal","volume":"75 4","pages":"Article 100824"},"PeriodicalIF":3.2,"publicationDate":"2025-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144072456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the Levels of Dental Anxiety in Greek Patients","authors":"Metaxia Kritsidima ,&nbsp;Sasha Scambler ,&nbsp;Koula Asimakopoulou","doi":"10.1016/j.identj.2025.04.006","DOIUrl":"10.1016/j.identj.2025.04.006","url":null,"abstract":"<div><h3>Introduction</h3><div>Dental anxiety is the apprehension experienced by an individual when confronted with matters related to dentistry, it impacts individuals' dental experiences and the dental healthcare providers’ professional lives, contributing to increased tension and potentially compromising performance. Levels of dental anxiety differ across cultures.</div></div><div><h3>Objective</h3><div>To collect normative data on levels of dental anxiety in Greece from a representative sample of people attending their usual dentist for a routine dental appointment.</div></div><div><h3>Methods</h3><div>A cross-sectional study was applied. 1313 Adults were recruited, as they attended 1 of 6 different dental settings and were scheduled for a routine dental appointment involving any of the following routine procedures: Check-up, hygiene, restoration, extraction, and pain relief. Their anxiety levels were assessed by applying the Modified Dental Anxiety Scale (MDAS), and by using the cut-off scores of 12 and 19 for assessing moderate and extreme anxiety, respectively.</div></div><div><h3>Results</h3><div>32.6% per cent of the respondents were found to be dentally anxious, out of which 8.2 % experienced high levels of dental anxiety. The mean MDAS score for the total study population was 10.36 (SD= 4.639). The findings indicated that females and first-time dental patients reported significantly higher levels of dental anxiety than males and repeat attendees. However, dental anxiety levels did not vary significantly across different dental procedures, age groups and dental settings. These results are in line with those reported by White in the U.S. population, demonstrating comparable levels of dental anxiety across both populations.</div></div><div><h3>Clinical Significance</h3><div>One in 3 patients visiting the dentist in Greece experience some level of dental anxiety. This finding underscores that dental anxiety is a prevalent public health concern in Greece. Dentists and healthcare providers should consider screening for dental anxiety that could lead to early identification and management.</div></div>","PeriodicalId":13785,"journal":{"name":"International dental journal","volume":"75 4","pages":"Article 100826"},"PeriodicalIF":3.2,"publicationDate":"2025-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144071270","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"DUWL Disinfectants and Pregnancy: Is Phenyalanine a Cause for Concern?","authors":"Mawj Alalawi,&nbsp;Haider Al-Saffar","doi":"10.1016/j.identj.2025.04.009","DOIUrl":"10.1016/j.identj.2025.04.009","url":null,"abstract":"","PeriodicalId":13785,"journal":{"name":"International dental journal","volume":"75 4","pages":"Article 100829"},"PeriodicalIF":3.2,"publicationDate":"2025-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144072457","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Can Large Language Models Serve as Reliable Tools for Information in Dentistry? A Systematic Review","authors":"Nora Alhazmi ,&nbsp;Aram Alshehri ,&nbsp;Fahad BaHammam ,&nbsp;Manju Philip ,&nbsp;Muhammad Nadeem ,&nbsp;Sanjeev Khanagar","doi":"10.1016/j.identj.2025.04.015","DOIUrl":"10.1016/j.identj.2025.04.015","url":null,"abstract":"<div><div>Large language models (LLMs) have gained popularity among dental students for generating subject-related answers. However, their widespread use raises significant concerns about misinformation. This systematic review aims to critically evaluate studies assessing the performance of LLMs in dentistry. A comprehensive electronic search was conducted in PubMed/Medline, Scopus, Embase, Web of Science, Google Scholar, and the Saudi Digital Library to identify studies published up to September 2024. The study quality was assessed using the Prediction Model Risk of Bias Assessment Tool (PROBAST). A total of 2030 studies have been identified. After removing 907 duplicate records, 1123 studies remained for screening. Ultimately, 31 studies met the inclusion criteria. Approximately half of these studies were classified as “high risk,” while the remainder were classified as “low risk.” The applicability of the findings was rated as “low concern.” The primary limitations of LLMs include their inability to specify information sources and their tendency to generate fabricated citations. Based on this review, LLMs hold promise as supplementary educational tools in dentistry. Evidence indicates that students using LLMs may achieve improved academic performance compared to traditional methods. However, concerns about occasional inaccuracies and unreliable citations underscore the need for further research, integration with validated sources, and adherence to ethical guidelines. Ultimately, LLMs should be viewed as complementary tools within dental education, with careful consideration of their limitations.</div></div>","PeriodicalId":13785,"journal":{"name":"International dental journal","volume":"75 4","pages":"Article 100835"},"PeriodicalIF":3.2,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144071380","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of Novel Boron-doped Mesoporous Bioactive Glass Nanoparticles Loaded Alginate Hydrogel in Dogs","authors":"Marwa Samir Naga ,&nbsp;Elbadawy Abdel Aziz Kamoun ,&nbsp;Maha Abdel Moaty ,&nbsp;Ahmed Zaki Ghareeb ,&nbsp;Mona Mohy El Din ,&nbsp;Samia Soliman Abdel Rehim Omar","doi":"10.1016/j.identj.2025.04.008","DOIUrl":"10.1016/j.identj.2025.04.008","url":null,"abstract":"<div><h3>Introduction</h3><div>Dentin regeneration is pivotal to preserve tooth vitality. This study aims to evaluate, histologically, the dentine regenerative potential of a novel injectable boron-doped, mesoporous, bioactive glass nanoparticle (BMBGNPs) loaded alginate hydrogel in dogs</div></div><div><h3>Methods</h3><div>The formulation and optimisation of the novel alginate/BMBG NPs (20 wt. %) loaded composite hydrogel were performed. Next, 66 teeth of 3 dogs were allocated into 3 groups (each including 22 teeth) according to post-operative follow-up period: group I: 2 weeks, group II: 4 weeks, and group III: 8 weeks. Each group was further subdivided according to pulpotomy filling material into two subgroups, with subgroup 1 (alginate/BMBGNPs (20 wt. %) loaded hydrogel) and subgroup 2 mineral trioxide aggregate (MTA). Pulp chambers were mechanically exposed through class V cavities. A complete pulpotomy was executed. The tested materials were positioned on the radicular pulp and finally covered with resin composite restorations. One dog was sacrificed after 2, 4, and 8 weeks. Teeth were prepared for histological evaluation assessing inflammatory cell response, pulp tissue organisation, and dentin bridge formation. The Mann-Whitney <em>U</em> test was employed to evaluate the scores of histological parameters between tested materials (<em>P</em> ≤ .05).</div></div><div><h3>Results</h3><div>Alginate/BMBG NPs (20 wt. %) loaded hydrogel showed normal pulp configuration at 2 and 4 weeks, which was enhanced after 8 weeks (<em>P</em> ≤ .05). Moderate inflammatory reaction was noted at 2 weeks, which was improved after 4 and 8 weeks (<em>P</em> ≤ .05). MTA group demonstrated less favourable pulpal response and inflammatory reaction with a statistically significant difference across all observational periods (<em>P</em> ≤ .05). After 8 weeks all teeth in group 1 exhibited the thickest dentin bridge (<em>P</em> ≤ .05).</div></div><div><h3>Conclusions</h3><div>Alginate/BMBG NPs (20 wt. %) loaded hydrogel offers the promise of regenerating dentin and maintaining pulp vitality reaching the desired level as an alternative to MTA.</div></div><div><h3>Clinical Relevance</h3><div>Alginate/BMBG NP loaded hydrogel is an alternative, reliable option for vital pulp therapy.</div></div>","PeriodicalId":13785,"journal":{"name":"International dental journal","volume":"75 4","pages":"Article 100828"},"PeriodicalIF":3.2,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144068103","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Emerging Roles of Periodontal Pathogen–Derived Outer Membrane Vesicles in NAFLD","authors":"Congcong Lv ,&nbsp;Kaikai Shi ,&nbsp;Yadong Guo,&nbsp;Zixin Guo,&nbsp;Pingchan Luo,&nbsp;Lijing Wang,&nbsp;Zhe Wu,&nbsp;Pei Yu","doi":"10.1016/j.identj.2025.03.029","DOIUrl":"10.1016/j.identj.2025.03.029","url":null,"abstract":"<div><div>The rising incidence of nonalcoholic fatty liver disease (NAFLD) poses a great socioeconomic burden worldwide. Also, periodontitis is the most common chronic inflammatory disease caused by a group of oral pathogens, affecting both oral health and systemic conditions, especially liver disease. Although accumulating evidence has elucidated an association between periodontal pathogens and NAFLD, the role of periodontal pathogen–derived outer membrane vesicles (OMVs) has not yet been clarified. In this comprehensive review, we aim to address this gap by summarising the progression and pathogenesis of NAFLD and revealing the relationship between periodontal disease and NAFLD multidimensionally. Additionally, this review sheds light on the multifunctional roles of periodontal pathogens OMVs and emphasises that periodontal pathogen–derived OMVs promote the development of NAFLD by stimulating Kupffer cells to produce inflammatory factors and inducing the activation of Hepatic stellate cells. However, it is still controversial whether periodontal pathogen–derived OMVs can be transferred to the liver through the bloodstream route or the oral-gut-liver axis. This highlights the pressing need for continued research efforts to develop new and optimised research schemes to observe the formation of the systemic distribution pathway of periodontal pathogen–derived OMVs. Finally, it is notable that there are currently no relevant clinical treatment guidelines to make specific provisions on controlling the level of periodontal pathogen–derived OMVs in patients with NAFLD. Guidelines developed based on our findings may contribute to the standardisation of practices. It can also provide effective strategies and potential therapeutic targets for NAFLD patients with periodontitis to alleviate the development of NAFLD diseases by inhibiting periodontal pathogens OMVs.</div></div>","PeriodicalId":13785,"journal":{"name":"International dental journal","volume":"75 4","pages":"Article 100825"},"PeriodicalIF":3.2,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144067986","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Facts and Fallacies of the Fluoride Controversy: A Contemporary Perspective","authors":"Lakshman Samaranayake ,&nbsp;Thantrira Porntaveetus ,&nbsp;James Tsoi ,&nbsp;Nozimjon Tuygunov","doi":"10.1016/j.identj.2025.04.013","DOIUrl":"10.1016/j.identj.2025.04.013","url":null,"abstract":"<div><div>Fluoride is a natural element recognized for its dental benefits. Fluoride prevents caries due to its antimicrobial activity, enhancing enamel resistance and promoting remineralization After decades of fluoridation of municipal water supplies to reduce dental caries, an intense debate has resurfaced regarding water fluoridation as a public health strategy to combat caries. This renewed discourse occurs against the backdrop of extensive data that clearly demonstrate the vital role of fluoride in caries prevention. Indeed, the Centres for Disease Control and Prevention has stated water fluoridation as 1 of the top ten public health interventions in the twentieth century. The FDI World Dental Federation also advocates systemic fluoride use as a cost-effective caries prevention strategy, and supports its inclusion in public health policies through a number of policy directives. This debate in the US has intensified as a Court concluded that water fluoridation poses unreasonable risks such as reduction of the intelligence quotient (IQ) in children. Additionally, recent statements from the leadership of the US Health and Human Services agency suggest that water fluoridation may cause more harm than good leading some states, like Utah, to revoke fluoridation mandates This article aims to provide a contemporary perspective on fluoride by revisiting its benefits, controversies, and potential risks. It highlights the dual role of fluoride in both individual oral care and public health and underscores the necessity for safe, equitable, and effective delivery strategies tailored to the needs of diverse populations, balancing its preventive advantages with potential health concerns. The review culminates with a summary of policy statements from the FDI World Dental Federation regarding the promotion of oral health through both systemic and topical fluoridation.</div></div>","PeriodicalId":13785,"journal":{"name":"International dental journal","volume":"75 4","pages":"Article 100833"},"PeriodicalIF":3.2,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143937675","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biodentine Counteracts the Aging Process of Human Dental Pulp Stem Cells Through Wnt/β-Catenin Pathway","authors":"Qi Zhang ,&nbsp;Shujie Cao ,&nbsp;Min Zhang ,&nbsp;Daming Wu ,&nbsp;Chengfei Zhang ,&nbsp;Jin Li","doi":"10.1016/j.identj.2025.03.028","DOIUrl":"10.1016/j.identj.2025.03.028","url":null,"abstract":"<div><h3>Objectives</h3><div>This study aimed to evaluate the effects of Biodentine on human Dental Pulp Stem Cells (hDPSCs) proliferation and senescence and explore the underlying anti-aging mechanism.</div></div><div><h3>Methods</h3><div>hDPSCs were isolated from two groups of patients aged 18 to 27 and 60 to 70 years and treated with Biodentine extract. The optimal concentration of Biodentine extract was determined using the CCK-8 assay. Biodentine treatment on hDPSCs proliferation and aging was assessed using qRT-PCR and Western blot. The small molecule inhibitor XAV939, targeting the Wnt/β-catenin signaling pathway, was added to investigate the anti-aging mechanism of Biodentine in hDPSCs.</div></div><div><h3>Results</h3><div>The isolated hDPSCs exhibited mesenchymal stem cells (MSCs) characteristics, and the proportion of S-phased cells decreased significantly in the aged group. Biodentine promoted hDPSCs proliferation, with the most pronounced effect observed at 0.2 mg/mL. qRT-PCR showed that Biodentine enhanced the expression of odonto/osteogenesis-related genes. Senescence-associated β-galactosidase staining and Western blot analysis further demonstrated the significant anti-aging effect of Biodentine on hDPSCs. Western blot revealed that Biodentine exerted its anti-aging effect through the Wnt/β-catenin signaling pathway.</div></div><div><h3>Conclusion</h3><div>The study demonstrated that Biodentine promotes proliferation and exerts anti-aging effects on hDPSCs through the Wnt/β-catenin pathway.</div></div>","PeriodicalId":13785,"journal":{"name":"International dental journal","volume":"75 4","pages":"Article 100819"},"PeriodicalIF":3.2,"publicationDate":"2025-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143937676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}