硅酸锶和硅酸钙基密封剂的细胞毒性和成骨潜能评价

IF 3.7 3区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

International dental journal Pub Date : 2025-09-01 DOI:10.1016/j.identj.2025.103869

Devin Chun Yue Kwan, Mingxin Hu, Junqing Liu, Chengfei Zhang, Angeline Hui Cheng Lee, Jeffrey Wen Wei Chang

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引用次数: 0

摘要

本研究旨在评估三种封口剂的细胞毒性和成骨潜能，包括一种基于硅酸锶的封口剂C-Root SP （C-R）和两种基于硅酸钙的封口剂iRoot SP （i-R）和AH Plus生物陶瓷封口剂（ahpcs），比较AH Plus （AHP）对MC3T3-E1前成骨细胞的影响。材料和方法：在培养基中洗脱标准密封片，使用CCK-8测定不同稀释度（1:1,1:2,1:5和1:10）评估细胞毒性。在添加1:5稀释的sealer提取物的成骨培养基中培养细胞，评估成骨分化。在第7天和第14天采用碱性磷酸酶（ALP）测定法和染色法测定碱性磷酸酶（ALP）活性。第21天用茜素红S染色观察矿化结节形成。采用RT‐qPCR检测成骨标志物（ALP、COL1A1、RUNX2）的基因表达。采用单向/双向方差分析和Tukey事后检验分析差异。P <； 0.05具有统计学意义。结果c - r、i-R和ahpcs的细胞活力明显高于AHP （P < 0.001）。所有封口剂在较高浓度（1:1和1:2稀释）下均表现出细胞毒性。与暴露于C-R、i-R和ahpcs的细胞相比，暴露于AHP的细胞中ALP活性显著降低（P < 0.01）。暴露于C-R、i-R和ahpcs的细胞比暴露于AHP的细胞表现出更高的矿化结节形成。C-R、i-R和ahpcs促进成骨分化，且成骨基因表达高于AHP （P < .001）。结论在研究范围内，C-R、i-R和ahpcs在较低浓度下与MC3T3-E1前成骨细胞具有生物相容性，并能增强其成骨潜能。硅酸锶基封口剂在体外显示出良好的生物反应和成骨活性，与硅酸钙基封口剂相当，表明其临床应用潜力。

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Evaluation of Cytotoxicity and Osteogenic Potential of Strontium Silicate and Calcium Silicate-Based Sealers

Objectives

This study aimed to evaluate the cytotoxicity and osteogenic potential of three sealers, including a strontium silicate-based sealer, C-Root SP (C-R), and two calcium silicate-based sealers, iRoot SP (i-R) and AH Plus Bioceramic Sealer (AHPbcs), compared with AH Plus (AHP) on MC3T3-E1 pre-osteoblasts.

Materials and Methods

Standardized sealer discs were eluted in a culture medium to assess cytotoxicity using the CCK-8 assay at various dilutions (1:1, 1:2, 1:5, and 1:10). Osteogenic differentiation was evaluated by culturing cells in osteogenic medium supplemented with 1:5 diluted sealer extract. Alkaline phosphatase (ALP) activity was assessed with an ALP assay and staining on days 7 and 14. Mineralized nodule formation was observed using Alizarin Red S staining on day 21. Gene expression of osteogenic markers (ALP, COL1A1, and RUNX2) was examined by RT‐qPCR. Differences were analysed using one-way/two-way variance analysis with the Tukey post-hoc test. Statistical significance was established at P < .05.

Results

C-R, i-R, and AHPbcs showed significantly higher cell viability than AHP (P < .001). All sealers exhibited cytotoxicity at higher concentrations (1:1 and 1:2 dilutions). ALP activity was significantly lower in cells exposed to AHP compared to cells exposed to C-R, i-R, and AHPbcs (P < .01). Cells exposed to C-R, i-R, and AHPbcs exhibited higher mineralized nodule formation than cells exposed to AHP. C-R, i-R, and AHPbcs enhanced osteogenic differentiation with higher osteogenic gene expression than AHP (P < .001).

Conclusions

Within the limitations of the study, C-R, i-R, and AHPbcs were biocompatible with MC3T3-E1 pre-osteoblasts at lower concentrations and were able to enhance their osteogenic potentials.

Clinical relevance

The strontium silicate-based sealer shows a favourable biological response and osteogenic activity in vitro, comparable to calcium silicate-based sealers, indicating its potential for clinical applications.

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来源期刊

International dental journal 医学-牙科与口腔外科

CiteScore

4.80

自引率

6.10%

发文量

159

审稿时长

63 days

期刊介绍： The International Dental Journal features peer-reviewed, scientific articles relevant to international oral health issues, as well as practical, informative articles aimed at clinicians.