International dental journal最新文献

{"title":"Effect of Life Course Adiposity on Bell’s Palsy Mediated by Glucose Levels","authors":"Xin Chen ,&nbsp;Kai Zhang ,&nbsp;Zheng Cheng ,&nbsp;Qianyi Wang ,&nbsp;Zhibai Zhao ,&nbsp;Qianglin Jiang","doi":"10.1016/j.identj.2025.100867","DOIUrl":"10.1016/j.identj.2025.100867","url":null,"abstract":"<div><h3>Introduction and aims</h3><div>Previous observational studies have reported an association between obesity and Bell’s palsy (BP), but the causality and the underlying mediating pathways remain unclear. We aimed to investigate the causal effects of adiposity across the life course on BP and to identify potential mediators in this relationship.</div></div><div><h3>Methods</h3><div>Utilizing summary statistics from genome-wide association studies predominantly of European ancestry, we conducted univariable and multivariable Mendelian randomization (MR) to estimate overall and independent effects of 5 adiposity-related traits (birth weight, childhood body mass index [BMI], adult BMI, adult body fat percentage, and adult waist circumference) on BP. Two-step MR was employed to assess the mediating role of metabolic and inflammatory traits. Sensitivity analyses were performed to evaluate robustness.</div></div><div><h3>Results</h3><div>Univariable MR revealed that genetically predicted adult BMI (OR: 1.29, 95% CI: 1.11-1.49, <em>P</em> = .001), body fat percentage (OR: 1.33, 95% CI: 1.08-1.65, <em>P</em> = .008), and waist circumference (OR: 1.29, 95% CI: 1.08-1.55, <em>P</em> = .005) were significantly associated with increased risk of BP. Multivariable MR confirmed an independent causal effect of adult BMI after adjusting for birth weight and childhood BMI. Two-step MR further identified glucose levels as a partial mediator, accounting for 23.8% (95% CI: 4.9%-42.6%) of the total effect. Findings were consistent across sensitivity analyses.</div></div><div><h3>Conclusion</h3><div>Higher genetically predicted adult BMI could increase the risk of BP, partly mediated by impaired glucose regulation.</div></div>","PeriodicalId":13785,"journal":{"name":"International dental journal","volume":"75 4","pages":"Article 100867"},"PeriodicalIF":3.2,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144322399","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Decoding Cellular Landscapes: Unravelling Periodontitis and Periapical Periodontitis at the Single-Cell Frontier","authors":"Yuan Li ,&nbsp;Jun Wang ,&nbsp;Yu Zhang ,&nbsp;Dengke Li ,&nbsp;Wuyang Zhang ,&nbsp;Xuebing Li ,&nbsp;Bolun Zhang ,&nbsp;Kaijin Hu ,&nbsp;Jianfei Yan ,&nbsp;Yang Xue","doi":"10.1016/j.identj.2025.100851","DOIUrl":"10.1016/j.identj.2025.100851","url":null,"abstract":"<div><h3>Objective</h3><div>Elucidate differences in key gene expression and cellular heterogeneity in alveolar bone between periodontitis (PD) and periapical periodontitis (PP) by analysing single-cell RNA sequencing data.</div></div><div><h3>Methods</h3><div>Single-cell RNA sequencing datasets for PD (GSE171213) and PP (GSE181688) were obtained from the Gene Expression Omnibus and analysed. Differentially expressed genes were identified in comparisons of PD versus healthy controls (HC), PP versus HC, and PD versus PP. Functional enrichment analyses were performed to explore heterogeneity. Key genes related to angiogenesis (ARGs), inflammation (IRGs), and phagocytosis (PRGs) were identified and combined to form PD-specific and PP-specific key gene sets. The expression of these key genes was validated by quantitative real-time polymerase chain reaction. Cell types were annotated using SingleR, and key cell types were further analysed for their proportions, intercellular communication, and gene expression patterns. Hallmark pathway analysis and cell cycle/differentiation assessments were conducted to understand functional heterogeneity.</div></div><div><h3>Results</h3><div>A total of 251 Differentially expressed genes associated with humoral immunity, B cell regulation, and leukocyte/lymphocyte activation were identified. Four PD-specific and 16 PP-specific key genes were found, and fibroblasts and macrophages were identified as the two key cell types. Fibroblast interactions were prominent, and cells in the G1 phase predominated. The key genes were highly expressed during early T cell and macrophage differentiation and during late fibroblast and plasma cell differentiation. Expression levels of these genes were highest in PP, intermediate in PD, and lowest in HC.</div></div><div><h3>Conclusion</h3><div>This study identified four PD-specific and 16 PP-specific key genes, as well as two pivotal cell types in alveolar bone inflammatory diseases. Investigating the functional diversity of these cell types could enhance insights into the pathogenesis of these conditions.</div></div><div><h3>Clinical Relevance</h3><div>The findings may guide clinical treatment strategies for alveolar bone involvement in PD and PP, and lay a theoretical foundation for exploring the underlying inflammatory mechanisms.</div></div>","PeriodicalId":13785,"journal":{"name":"International dental journal","volume":"75 4","pages":"Article 100851"},"PeriodicalIF":3.2,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144306439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inhibition of Dynamin-Related Protein 1-Dependent Mitochondrial Fission Ameliorates Apical Periodontitis by Attenuating NLRP3 Inflammasome-Mediated M1 Macrophage Polarisation","authors":"Yao Yang ,&nbsp;Min Li ,&nbsp;Lifei Pan ,&nbsp;Yifei Tang ,&nbsp;Yuting Wang ,&nbsp;Xiaoyue Guan ,&nbsp;Tiezhou Hou ,&nbsp;Siyu Hou","doi":"10.1016/j.identj.2025.100853","DOIUrl":"10.1016/j.identj.2025.100853","url":null,"abstract":"<div><h3>Introduction and Aims</h3><div>The aim of this study was to determine if Dynamin-related protein 1‌ (Drp1) -dependent mitochondrial fission is involved in the pathological process of apical periodontitis and elucidate the underlying mechanisms.</div></div><div><h3>Methods</h3><div>Immunohistochemistry, immunofluorescence, and western blotting assessed CD86 expression, NLRP3/Caspase1/IL-1β activation, and mitochondrial dynamics-related proteins in human periapical macrophages. In vitro apical periodontitis (AP) models used macrophages stimulated with <em>P. gingivalis</em> LPS (Pg-LPS), pretreated with/without Drp1 inhibitor Mdivi-1 or NLRP3 inhibitor MCC950. CD86, NLRP3/Caspase1/IL-1β, mitochondrial dynamics proteins, TNF, and IL-6 were quantified via western blot, RT-qPCR, or immunofluorescence. ROS (DCFH-DA), ATP (commercial kit), mitochondrial membrane potential (JC-1), and morphology (TEM, MitoTracker/immunofluorescence) were analysed. Multimodal approaches explored Drp1-mediated mitochondrial fission and NLRP3 inflammasome-dependent M1 polarisation. Mdivi-1-treated AP models were established to dissect Drp1’s role in pathogenesis.</div></div><div><h3>Results</h3><div>Our findings indicate that the Drp1-mediated excessive mitochondrial fission is present in human periapical lesions. Moreover, there was a positive correlation between the p-Drp1^(Ser616) and the elevation of NLRP3, Cleaved-Caspase1, and CD86. In vitro experiments demonstrated that Mdivi-1 effectively inhibited the Pg-LPS induced abnormal mitochondrial fragmentation dependent on p-Drp1^(Ser616), rescued mitochondrial dysfunction, and further suppressed the activation of NLRP3/Caspase1/IL1β and the expression of CD86. In vivo experiments showed that Mdivi-1 treatment could significantly alleviate the inflammatory bone erosion at the apex in a murine AP model by inhibiting macrophage polarisation and the NLRP3 inflammatory pathway.</div></div><div><h3>Conclusion</h3><div>Drp1-mediated excessive mitochondrial fission plays a crucial role in the development of AP by promoting NLRP3 signaling pathway-dependent macrophage M1 polarisation. Targeting Drp1 may serve as a potential therapeutic strategy to prevent AP development, highlighting its clinical translational value.</div></div><div><h3>Clinical Relevance</h3><div>Mdivi-1 can restore mitochondrial dynamics homeostasis in periapical macrophages, inhibit NLRP3/M1 polarisation, and reduce bone resorption. This suggests that Mdivi-1 might be a promising agent for management of AP in future.</div></div>","PeriodicalId":13785,"journal":{"name":"International dental journal","volume":"75 4","pages":"Article 100853"},"PeriodicalIF":3.2,"publicationDate":"2025-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144280437","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An Artificial Intelligence Solution for Automated Dental Inspection and Charting","authors":"M.A. Moufti ,&nbsp;M. Abu Talib ,&nbsp;S. Al Mokdad ,&nbsp;A. Alhouria ,&nbsp;M. Alsaffarini ,&nbsp;N. Almahmeed ,&nbsp;S. Eldessouky ,&nbsp;W. Kharoufah ,&nbsp;Q. Nasir","doi":"10.1016/j.identj.2025.100849","DOIUrl":"10.1016/j.identj.2025.100849","url":null,"abstract":"<div><h3>Background</h3><div>Visual inspection and documentation (charting) of findings are critical components in dental practice. However, manual charting is tedious, time consuming, and prone to errors. Attempts to automate charting using AI have focused on computer vision of radiographs, which does not replace visual inspection and has limitations in community screening settings. We aim to develop a deep-learning-based real-time charting using intra-oral videos. This study encompasses tooth identification, the initial phase of our development process.</div></div><div><h3>Methods</h3><div>100 intra-oral videos were recorded, annotated using CVAT software, and split into training and testing datasets (80:20 ratio) to develop an object detection YOLOv7x model on a Convolutional Neural Network (CNN). The model was evaluated by its ability to accurately detect each tooth's ID (class) and position using the mean average precision (AP), which encases a confusion matrix, Precision and Recall, based on a minimum overlap of 50% between manual and automated annotation (Intersection over Union IoU = 0.5).</div></div><div><h3>Results</h3><div>Of the 186,332 captured image frames; 8747 were annotated, with data augmentation yielding 17,494 images. AP varied among tooth types; mAP at 0.5 IoU reached 78.8%, with Precision and Recall at 72.5% and 77.9% respectively.</div></div><div><h3>Conclusion</h3><div>To the best of our knowledge, this is the first study to utilize real-time videos for this purpose. This research presents a novel approach to automate dental inspection and charting, offering the potential to reduce clinical time and costs, improve patient experience and outcomes, and enhance patient access to dental care.</div></div>","PeriodicalId":13785,"journal":{"name":"International dental journal","volume":"75 4","pages":"Article 100849"},"PeriodicalIF":3.2,"publicationDate":"2025-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144280430","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Value Analysis of Preoperative Peripheral Blood Markers in the Identification of Benign From Malignant Parotid Gland Tumors","authors":"Zheng Ye,&nbsp;Hui Dong,&nbsp;Fuyong Sui","doi":"10.1016/j.identj.2025.100854","DOIUrl":"10.1016/j.identj.2025.100854","url":null,"abstract":"<div><h3>Objectives</h3><div>To assess the value of preoperative peripheral blood inflammatory and nutritional parameters in distinguishing between benign and malignant parotid gland tumors.</div></div><div><h3>Methods</h3><div>A retrospective analysis was conducted on 79 patients with malignant parotid gland tumors and 79 patients with benign parotid gland tumors who were treated at the Second Hospital of Dalian Medical University from January 2015 to December 2024. Preoperative levels of total protein (TP), prealbumin (PA), albumin (Alb), albumin-to-globulin ratio (AGR), apolipoprotein A1-to-apolipoprotein B ratio (ApoA1/ApoB), and platelet-to-lymphocyte ratio (PLR) were compared between the malignant and benign tumor groups. The diagnostic value of these parameters in differentiating between benign and malignant parotid tumors was evaluated.</div></div><div><h3>Results</h3><div>The ApoA1/ApoB ratio and PLR were significantly higher in the malignant tumor group compared to the benign tumor group (<em>P &lt; .</em>05). Conversely, TP, Alb, PA, and AGR were significantly lower in the malignant tumor group than in the benign tumor group (<em>P &lt; .</em>05). Binary logistic regression analysis identified TP, PA, AGR, and ApoA1/ApoB as independent risk factors for parotid malignancy (<em>P &lt; .</em>05). The optimal cut-off values for diagnosing parotid malignancy were 65.75 for TP, 248.46 for PA, 1.55 for AGR, and 1.45 for ApoA1/ApoB.</div></div><div><h3>Conclusions</h3><div>Preoperative peripheral blood inflammatory and nutritional markers may serve as valuable indicators for differentiating between benign and malignant parotid gland tumors, providing a potential adjunctive diagnostic tool. However, further large-scale studies are required to validate these findings.</div></div>","PeriodicalId":13785,"journal":{"name":"International dental journal","volume":"75 4","pages":"Article 100854"},"PeriodicalIF":3.2,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144271229","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical and Preclinical Postgraduate Training in Endodontic Education: A Transnational Exploratory Survey","authors":"Venkateshbabu Nagendrababu ,&nbsp;Vellore Kannan Gopinath ,&nbsp;Mohannad Nassar ,&nbsp;Naresh Shetty ,&nbsp;Anil Kishen ,&nbsp;Paul V. Abbott ,&nbsp;Henry F. Duncan","doi":"10.1016/j.identj.2025.100861","DOIUrl":"10.1016/j.identj.2025.100861","url":null,"abstract":"<div><h3>Introduction and aims</h3><div>Endodontic postgraduate programmes should offer students a wide range of experiences and training in all facets of the specialisation. This study aimed to assess the current postgraduate education practices in endodontics across dental schools worldwide.</div></div><div><h3>Methods</h3><div>The current survey consisted of an online questionnaire that had been validated and piloted. It comprised 68 questions in three sections: general information about the programme, preclinical education, and clinical education. The survey included faculty members who teach postgraduate endodontics from one dental school in each participating country. The data was presented using simple descriptive statistics.</div></div><div><h3>Results</h3><div>In total, 33 faculty members, with a response rate of 85%, from different countries completed the survey. The majority of dental schools that participated in the survey offer a 3-year postgraduate programme. The postgraduate students develop abilities in various instrumentation techniques, irrigation devices, sealers/cements, root canal filling techniques, surgery, and microscope use. Most of the schools conduct a final-year exit examination.</div></div><div><h3>Conclusions</h3><div>Postgraduate education differs globally in its preclinical and clinical training. The survey offers initial insights into postgraduate endodontic education and can help establish mutual educational standards and key focus areas.</div></div><div><h3>Clinical relevance</h3><div>The curricula for preclinical and clinical education in postgraduate endodontics programmes vary globally.</div></div>","PeriodicalId":13785,"journal":{"name":"International dental journal","volume":"75 4","pages":"Article 100861"},"PeriodicalIF":3.2,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144271230","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advancements and Trends in Electrochemical Biosensors for Saliva-Based Diagnosis of Oral Diseases: A Bibliometric Analysis (2000–2023)","authors":"Qian Tang ,&nbsp;Fushen Zhang ,&nbsp;Weiye Xu ,&nbsp;Zejun Deng ,&nbsp;Zhangui Tang ,&nbsp;Jufang Huang","doi":"10.1016/j.identj.2025.100840","DOIUrl":"10.1016/j.identj.2025.100840","url":null,"abstract":"<div><h3>Background</h3><div>Oral diseases, such as dental caries, periodontal diseases, and oral cancers, represent significant global public health challenges. With the COVID-19 pandemic, traditional diagnostic methods have limitations, thus highlighting the need for innovative, early, and non-invasive diagnostic approaches. Analyse and summarise publications globally through a comprehensive bibliometric study to help researchers suggest possible directions for future research.</div></div><div><h3>Objective</h3><div>The purpose of the study was to evaluate global academic productivity, impact, and collaboration of electrochemical biosensors for oral diseases utilising bibliometrics based on annual number of publications, countries and regions, institution, authors, journals, citations and co-occurrences of author keywords over the last 20 years.</div></div><div><h3>Methods</h3><div>This study employs bibliometric analysis to assess the research of electrochemical biosensors for oral diseases in the Web of Science from 2000 to 2023. Utilising VOSviewer, CiteSpace software, and Microsoft Excel, we conducted a bibliometric and visualised analysis of electrochemical biosensors for oral diseases.</div></div><div><h3>Results</h3><div>The quantity of pertinent publications in this research domain displays a fluctuating but overall upward trend. In aggregate, there are 341 articles and 82 reviews, encompassing research contributions from 55 countries, 649 institutions, and involving 2068 authors. Among these publications, China, the USA, and India emerged as the predominant contributing nations. Predominantly, articles found their publication venue in “Biosensors &amp; Bioelectronics.” Notably, the author with the highest number of publications and most influence is Wang, Joseph S. The top 3 keywords include “biosensor,”“sensor,”“saliva.”</div></div><div><h3>Conclusion</h3><div>In this investigation, statistical analysis and network visualisation were conducted to reveal the research progress, trends, and trending topics on electrochemical biosensors for oral diseases via a thorough bibliometric analysis. We found that Despite these challenges, electrochemical biosensors hold significant promise for transforming oral disease diagnostics. Overcoming current technical barriers will improve both oral and systemic health outcomes.</div></div>","PeriodicalId":13785,"journal":{"name":"International dental journal","volume":"75 4","pages":"Article 100840"},"PeriodicalIF":3.2,"publicationDate":"2025-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144262064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Secular Trends in the Incidence, Prevalence, and Disability-Adjusted Life Years of Caries in Permanent Teeth in Six Asian Countries: 1990 to 2021","authors":"Min Deng ,&nbsp;Xihua Yuan ,&nbsp;Xiaoying Zhu ,&nbsp;Jiaqiang Wei ,&nbsp;Minsi Li ,&nbsp;Ziyi Yang ,&nbsp;Na Yu ,&nbsp;Xiaofeng Qin","doi":"10.1016/j.identj.2025.100841","DOIUrl":"10.1016/j.identj.2025.100841","url":null,"abstract":"<div><h3>Introduction and aims</h3><div>Untreated dental caries in permanent teeth is a significant public health problem, particularly in Asia. This study compared the long-term trends in the incidence, prevalence, and disability-adjusted life year (DALY) rates of untreated dental caries in permanent teeth between China, India, Japan, South Korea, Thailand, and Singapore from 1990 to 2021. This selection provides a comprehensive representation of economic, healthcare, and demographic diversity in Asia, making our findings widely applicable.</div></div><div><h3>Methods</h3><div>Data were obtained from the Global Burden of Disease Study 2021. Changes in both the annual percentage and average annual percentage were calculated using joinpoint regression analysis. Independent age, period, and cohort effects were estimated by age–period–cohort analysis.</div></div><div><h3>Results</h3><div>From 1990 to 2021, the overall age-standardized incidence rate showed an increasing trend in China, India, and Thailand and a decreasing trend in Japan, Korea, and Singapore. The standardized prevalence and DALY rates decreased among countries, with Korea having the highest prevalence rate and India and Thailand having the highest prevalence and DALY rates. On age–period–cohort analysis, (1) the 20 to 24-year age group carried the highest caries risk, which declined with increasing age (age effect); (2) an overall decreasing or flat trend was observed (period effect); and (3) Japan, Singapore, and South Korea showed increasing incidence, prevalence, and DALY rates, whereas China, India, and Thailand exhibited decreasing prevalence and DALY rates (cohort effect).</div></div><div><h3>Conclusion</h3><div>This study revealed notable regional variations in the burden of untreated dental caries in permanent teeth across Asia from 1990 to 2021. These findings provide valuable insights for policymakers and healthcare professionals to develop targeted and evidence-based oral health strategies in the region.</div></div><div><h3>Clinical Relevance</h3><div>These findings underscore the need to develop targeted oral health solutions in Asia.</div></div>","PeriodicalId":13785,"journal":{"name":"International dental journal","volume":"75 4","pages":"Article 100841"},"PeriodicalIF":3.2,"publicationDate":"2025-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144262065","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mediating Effects of Systemic Inflammation on the Association Between Body Roundness Index and Periodontitis in US Adults","authors":"Hui Zhang ,&nbsp;Zhengyun Ren ,&nbsp;Xi Peng ,&nbsp;Tailin Guo","doi":"10.1016/j.identj.2025.04.012","DOIUrl":"10.1016/j.identj.2025.04.012","url":null,"abstract":"&lt;div&gt;&lt;h3&gt;Introduction and Aim&lt;/h3&gt;&lt;div&gt;Periodontitis is a highly prevalent chronic inflammatory disease affecting periodontal tissues. While the Body Roundness Index (BRI), has emerged as a novel anthropometric measure for evaluating obesity-related health risks, its relationship with periodontal health remains unexplored. Although systemic inflammation is recognized as a key role in both obesity and periodontitis. However, whether BRI affects periodontitis, the mediating role of systemic inflammation in BRI-related periodontitis has not been elucidated.&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Materials and Methods&lt;/h3&gt;&lt;div&gt;Data were derived from the National Health and Nutrition Examination Survey (NHANES) 2009-2014, comprising 8415 participants aged ≥18 years. We analysed BRI's correlation with periodontal disease using binary logistic regression models. Restricted Cubic Spline (RCS) modelling explored nonlinear patterns. The predictive performance of BRI for periodontitis was compared with traditional anthropometric indices using Receiver Operating Characteristic (ROC) curves. Mediation models assessed how systemic inflammation (SII, SIRI) bridges the BRI-periodontitis link.&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Results&lt;/h3&gt;&lt;div&gt;In the fully adjusted model, participants in higher BRI quartiles showed progressively increased odds of periodontitis compared with those in the lowest quartile (Q1), with odds ratios of 1.33 (95% CI: 1.07-1.65, &lt;em&gt;P&lt;/em&gt; = .010) for Q2, 1.48 (95% CI: 1.16-1.88, &lt;em&gt;P&lt;/em&gt; = .004) for Q3, and 1.70 (95% CI: 1.20-2.40, &lt;em&gt;P&lt;/em&gt; = .010) for Q4. RCS analysis indicated a linear relationship between BRI and periodontitis risk (nonlinearity &lt;em&gt;P&lt;/em&gt; = .201). ROC curves revealed that BRI demonstrated superior predictive performance for periodontitis compared to BMI. The mediation analysis indicated that SII (5.37%, 95% CI: 0.86%-15.02%, &lt;em&gt;P&lt;/em&gt; &lt; .001) and SIRI (8.92%, 95% CI: 2.73%-22.13%, &lt;em&gt;P&lt;/em&gt; &lt; .05) partially mediated the BRI-periodontitis association.&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Conclusions&lt;/h3&gt;&lt;div&gt;Our study demonstrates that elevated BRI is positive associated with an increased risk of periodontitis. Systemic inflammation, as reflected by SII and SIRI, partially mediates this relationship.&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;ClinicalRelevance&lt;/h3&gt;&lt;div&gt;Body Roundness Index (BRI), has emerged as a novel anthropometric indicator that more precisely estimates visceral adiposity and body fat percentage. Obesity, particularly the dysfunction of adipose tissue in visceral obesity, leads to the secretion of many pro-inflammatory factors, triggering systemic inflammatory responses. Systemic inflammation is recognized as a key role in both obesity and periodontitis. However, whether BRI affects periodontitis, the mediating role of systemic inflammation in BRI-related periodontitis has not been elucidated. This study supports that BRI is significantly associated with an increased risk of periodontitis, and systemic inflammation partially mediates this relationship. These ","PeriodicalId":13785,"journal":{"name":"International dental journal","volume":"75 4","pages":"Article 100832"},"PeriodicalIF":3.2,"publicationDate":"2025-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144241905","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Expression of Carnitine Palmitoyltransferase I Isotypes in Gingival Tissues of Human Periodontitis","authors":"Yue Shen ,&nbsp;Lu Lin ,&nbsp;Weijun Yu,&nbsp;Zhurong Cui,&nbsp;Tingting Lin,&nbsp;Shucheng Hu,&nbsp;Yuanjie Shi,&nbsp;Yuting Gu,&nbsp;Min Jin,&nbsp;Eryi Lu","doi":"10.1016/j.identj.2025.100837","DOIUrl":"10.1016/j.identj.2025.100837","url":null,"abstract":"<div><h3>Introduction and aims</h3><div>Carnitine palmitoyltransferase I (CPT1), the rate-limiting enzyme of fatty acid oxidation, plays a crucial role in inflammation. However, the relation between CPT1 and periodontitis is obscure. This study aimed to reveal the expression pattern of CPT1 (CPT1A, CPT1B, CPT1C) in periodontitis and their correlation with clinical parameters.</div></div><div><h3>Methods</h3><div>The public dataset was used to identify biological processes related to lipid metabolism and validate the differential expression of CPT1 between healthy and periodontitis groups. Forty-seven healthy gingival samples and 47 periodontitis gingival samples were gathered. Subsequently, real-time quantitative polymerase chain reaction was used to measure CPT1 expression. Their protein levels were assessed using western blotting and histological analysis. Single-cell analysis using GSE164241 was conducted to observe CPT1A and CPT1C expression in different cell types, followed by immunofluorescence validation. Finally, the correlation of CPT1A and CPT1C with clinical parameters was estimated, and receiver operating characteristic curves were constructed.</div></div><div><h3>Results</h3><div>Most biological processes related to lipid metabolism shared the gene <em>CPT1A</em>, and the expression of CPT1A and CPT1C in the periodontitis group was upregulated. Besides, both CPT1A and CPT1C were expressed mainly in endothelial cells, and their expression was elevated in endothelial cells of periodontitis. Furthermore, CPT1A and CPT1C were correlated with clinical parameters, and CPT1A could serve as a diagnostic marker of periodontitis.</div></div><div><h3>Conclusions</h3><div>Our findings elucidated the expression of CPT1 in gingival tissues of periodontitis and demonstrated the association of CPT1 with periodontitis.</div></div><div><h3>Clinical relevance</h3><div>This study found the positive correlation of the expression of CPT1A and CPT1C in gingival tissues with clinical parameters, which might lay a foundational framework for further elucidating the underlying mechanisms of lipid metabolism and periodontitis and pave the way for exploring potential therapeutic targets.</div></div>","PeriodicalId":13785,"journal":{"name":"International dental journal","volume":"75 4","pages":"Article 100837"},"PeriodicalIF":3.2,"publicationDate":"2025-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144255409","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}