人牙周炎牙龈组织中肉毒碱棕榈酰基转移酶I同型的表达

IF 3.2 3区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

International dental journal Pub Date : 2025-06-10 DOI:10.1016/j.identj.2025.100837

Yue Shen , Lu Lin , Weijun Yu, Zhurong Cui, Tingting Lin, Shucheng Hu, Yuanjie Shi, Yuting Gu, Min Jin, Eryi Lu

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引用次数: 0

摘要

scnitine palmitoyltransferase I （CPT1）是脂肪酸氧化的限速酶，在炎症中起着至关重要的作用。然而，CPT1与牙周炎的关系尚不清楚。本研究旨在揭示CPT1 （CPT1A、CPT1B、CPT1C）在牙周炎中的表达规律及其与临床参数的相关性。方法利用公共数据集确定脂质代谢相关的生物学过程，并验证CPT1在健康组和牙周炎组之间的差异表达。采集健康牙龈标本47份，牙周炎牙龈标本47份。随后，采用实时定量聚合酶链反应检测CPT1的表达。采用western blotting和组织学分析评估各组蛋白水平。采用GSE164241进行单细胞分析，观察CPT1A和CPT1C在不同细胞类型中的表达情况，并进行免疫荧光验证。最后估计CPT1A、CPT1C与临床参数的相关性，构建患者工作特征曲线。结果与脂质代谢相关的大多数生物学过程与CPT1A基因共享，牙周炎组CPT1A和CPT1C表达上调。CPT1A和CPT1C主要在内皮细胞中表达，在牙周炎内皮细胞中表达升高。CPT1A和CPT1C与临床参数相关，CPT1A可作为牙周炎的诊断指标。结论研究结果证实了CPT1在牙周炎牙龈组织中的表达，并证实了CPT1与牙周炎的相关性。临床相关性本研究发现牙龈组织中CPT1A和CPT1C的表达与临床参数呈正相关，这可能为进一步阐明脂质代谢与牙周炎的潜在机制奠定基础框架，并为探索潜在的治疗靶点铺平道路。

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Expression of Carnitine Palmitoyltransferase I Isotypes in Gingival Tissues of Human Periodontitis

Introduction and aims

Carnitine palmitoyltransferase I (CPT1), the rate-limiting enzyme of fatty acid oxidation, plays a crucial role in inflammation. However, the relation between CPT1 and periodontitis is obscure. This study aimed to reveal the expression pattern of CPT1 (CPT1A, CPT1B, CPT1C) in periodontitis and their correlation with clinical parameters.

Methods

The public dataset was used to identify biological processes related to lipid metabolism and validate the differential expression of CPT1 between healthy and periodontitis groups. Forty-seven healthy gingival samples and 47 periodontitis gingival samples were gathered. Subsequently, real-time quantitative polymerase chain reaction was used to measure CPT1 expression. Their protein levels were assessed using western blotting and histological analysis. Single-cell analysis using GSE164241 was conducted to observe CPT1A and CPT1C expression in different cell types, followed by immunofluorescence validation. Finally, the correlation of CPT1A and CPT1C with clinical parameters was estimated, and receiver operating characteristic curves were constructed.

Results

Most biological processes related to lipid metabolism shared the gene CPT1A, and the expression of CPT1A and CPT1C in the periodontitis group was upregulated. Besides, both CPT1A and CPT1C were expressed mainly in endothelial cells, and their expression was elevated in endothelial cells of periodontitis. Furthermore, CPT1A and CPT1C were correlated with clinical parameters, and CPT1A could serve as a diagnostic marker of periodontitis.

Conclusions

Our findings elucidated the expression of CPT1 in gingival tissues of periodontitis and demonstrated the association of CPT1 with periodontitis.

Clinical relevance

This study found the positive correlation of the expression of CPT1A and CPT1C in gingival tissues with clinical parameters, which might lay a foundational framework for further elucidating the underlying mechanisms of lipid metabolism and periodontitis and pave the way for exploring potential therapeutic targets.

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来源期刊

International dental journal 医学-牙科与口腔外科

CiteScore

4.80

自引率

6.10%

发文量

159

审稿时长

63 days

期刊介绍： The International Dental Journal features peer-reviewed, scientific articles relevant to international oral health issues, as well as practical, informative articles aimed at clinicians.