International dental journal最新文献

{"title":"Global Burden of Oral Diseases in Adolescents and Young Adults Aged 10–24 Years","authors":"Wei Lu ,&nbsp;Bing Wang ,&nbsp;Mi Du ,&nbsp;Shaohua Ge","doi":"10.1016/j.identj.2025.100875","DOIUrl":"10.1016/j.identj.2025.100875","url":null,"abstract":"<div><h3>Objectives</h3><div>This study aims to analyse global trends in the burden of oral diseases, including dental caries and noncarious oral diseases (NCODs), in individuals aged 10 to 24 from 1990 to 2021 and project future trends up to 2050.</div></div><div><h3>Methods</h3><div>Using data from the Global Burden of Disease Study (GBD) 2021, this trend analysis examines the number, age-standardised rates (ASRs), and average annual percentage changes (AAPCs) in prevalence, incidence, and disability-adjusted life years for oral diseases across different age groups, sexes, social-development index (SDI) levels, regions, and nations. Joinpoint regression was applied to identify key inflection points, while Bayesian modelling was used to project future trends.</div></div><div><h3>Results</h3><div>Globally, the ASR of oral disease prevalence slightly decreased from 35,408 per 100,000 populations (95% CI: 25,412-47,171) in 1990 to 34,004 per 100,000 population (95% CI: 25,255-44,634) in 2021, with an AAPC of –0.13 (95% CI: –0.16 to –0.10). Dental caries saw a reduction in prevalence and burden, while NCODs increased. Males experienced a faster decline than females, who consistently had higher prevalence and burden. Adolescents aged 10 to 14 years showed the most significant decline. Central Europe experienced the most significant regional reduction, while Greece saw the largest national decline in the prevalence of oral diseases. Higher SDI strata were associated with lower prevalence, with high-SDI countries showing the greatest decline. Projections indicate a slight decrease in prevalence by 2050, with the overall burden remaining unchanged.</div></div><div><h3>Conclusions</h3><div>Despite the decline in prevalence, the burden of oral diseases remains, highlighting the need for more effective prioritisation of oral health care for adolescents and young adults.</div></div><div><h3>Clinical Significance</h3><div>The persistent burden of oral diseases and disparities in oral health are evident across different sexes, age groups, SDI levels, regions, and nations. This highlights the ongoing need for increased attention to oral health among adolescents and young adults, particularly for females, older adolescents, and those in low- and middle-income countries.</div></div>","PeriodicalId":13785,"journal":{"name":"International dental journal","volume":"75 4","pages":"Article 100875"},"PeriodicalIF":3.2,"publicationDate":"2025-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144480147","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Failure Risk of Composite Resin and Amalgam Restorations: A Systematic Review and Meta-Analysis","authors":"Woroud Al-Sulimmani ,&nbsp;Asmaa Al-Rasheed ,&nbsp;Hebah Al-Daraan ,&nbsp;Muna Al-Mutairi ,&nbsp;Yash Brahmbhatt ,&nbsp;Hesham Al-Hazmi ,&nbsp;Hend Al-Qaderi","doi":"10.1016/j.identj.2025.100871","DOIUrl":"10.1016/j.identj.2025.100871","url":null,"abstract":"<div><h3>Objective</h3><div>This systematic review and meta-analysis were conducted to compare the failure risk between amalgam and composite resin materials in permanent posterior teeth.</div></div><div><h3>Material and methods</h3><div>Study eligibility requirements included clinical trials and observational studies with at least 12 months of follow-up. English-language studies from 1990 onwards were the only studies included. This review follows the <em>Cochrane Handbook for Systematic Reviews of Interventions</em> and the guidelines of the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA). Our search strategy included using the following databases: PubMed, Cochrane, and Google Scholar. The primary outcome was restoration failures, defined as restoration replacements, tooth and restoration fractures, secondary caries, postoperative sensitivity, and toothaches. We conducted a random-effects meta-analysis to determine the risk ratio (RR) of the included studies, and publication bias was assessed. The Cochrane Risk of Bias tool was employed to evaluate the quality of the clinical trials, while the Newcastle–Ottawa scale was used to assess the quality of other studies.</div></div><div><h3>Results</h3><div>The results were derived from 13 studies. The failure proportion for amalgam ranged from 0% to 50.0%, while that of composite resin restorations ranged from 0% to 62.7%. The meta-analysis did not find any statistically significant difference in failure risk between amalgam and composite resin restorations (RR: 0.96, 95% confidence intervals: 0.68-1.34). The Egger’s test results did not show any significant evidence of publication bias in the meta-analysis (<em>P</em> &gt; .05).</div></div><div><h3>Conclusion</h3><div>This review did not reveal any statistically significant difference in the RR between composite resin and amalgam restorations. However, in their analyses, the 13 studies used varying definitions of failure and did not account for some important factors that might have influenced restoration failures. Future reviews need to account for other influential variables that contributed to restoration failures.</div></div>","PeriodicalId":13785,"journal":{"name":"International dental journal","volume":"75 4","pages":"Article 100871"},"PeriodicalIF":3.2,"publicationDate":"2025-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144480204","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"AI-Powered Problem- and Case-based Learning in Medical and Dental Education: A Systematic Review and Meta-analysis","authors":"Hongxia Wei ,&nbsp;Yuguo Dai ,&nbsp;Kaiting Yuan ,&nbsp;Kar Yan Li ,&nbsp;Kuo Feng Hung ,&nbsp;Elaine Mingxin Hu ,&nbsp;Angeline Hui Cheng Lee ,&nbsp;Jeffrey Wen Wei Chang ,&nbsp;Chengfei Zhang ,&nbsp;Xin Li","doi":"10.1016/j.identj.2025.100858","DOIUrl":"10.1016/j.identj.2025.100858","url":null,"abstract":"<div><h3>Introduction and Aims</h3><div>Advances in artificial intelligence (AI) technology have generated a revolution in medical and dental education, which may offer promising solutions to tackle the challenges of traditional problem-based learning (PBL) and case-based learning (CBL). The objective of this study was to assess the available evidence concerning AI-powered PBL/CBL on students’ knowledge acquisition, clinical reasoning capability and satisfaction.</div></div><div><h3>Methods</h3><div>An electronic search was carried out on PubMed, MEDLINE, the Cochrane Central Register of Controlled Trials and Web of Science. Clinical trials published in English with full text available, which implemented AI technologies in PBL/CBL in the medical/dental field and evaluated knowledge acquisition, clinical reasoning and/or satisfaction were included. The quality assessment was conducted using RoB 2 by two calibrated assessors. Data synthesis and meta-analysis were performed, the standardised mean difference (SMD) or standardised mean (SM) and 95% confidence intervals (CIs) were calculated, and heterogeneity was quantified.</div></div><div><h3>Results</h3><div>Six randomized controlled trials were included, with an overall risk of bias judged to have ‘some concerns’. For knowledge acquisition, 4 studies were included in the meta-analysis. A low heterogeneity (I² = 20%) was detected and a fixed-effect model was utilised. Compared with the control group, the AI intervention significantly improved knowledge acquisition by 46% (95% Cls [0.18-0.73], <em>P</em> = .001). For clinical reasoning capability, due to methodological and measurement heterogeneity among studies, statistical analysis was not feasible. Three studies were selected for the meta-analysis of students’ satisfaction. Heterogeneity was moderate (I² = 32%), and a generic inverse variance method was selected. The pooled SM score was 0.7 (95% Cls [0.47-0.92]), and the overall effect was statistically significant (<em>P</em> &lt; .00001).</div></div><div><h3>Conclusion</h3><div>Despite limitations such as the limited number of included studies and the overall risk of bias concerns, AI-powered PBL/CBL has the potential to enhance students’ knowledge acquisition and learner satisfaction compared to traditional learning approaches.</div></div><div><h3>Clinical Relevance</h3><div>Not applicable.</div></div>","PeriodicalId":13785,"journal":{"name":"International dental journal","volume":"75 4","pages":"Article 100858"},"PeriodicalIF":3.2,"publicationDate":"2025-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144491633","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biomechanical Analysis of Various Connector Designs of Dental Implant Complex: A Numerical Finite Element Study","authors":"Shawkat A Elsheikh ,&nbsp;Mohamed I El-Anwar ,&nbsp;Tao Hong ,&nbsp;Christoph Bourauel ,&nbsp;Abdulaziz Alhotan ,&nbsp;Noha M Anany ,&nbsp;Islam G Shahin ,&nbsp;Al-Hassan Diab ,&nbsp;Tarek M Elshazly","doi":"10.1016/j.identj.2025.100873","DOIUrl":"10.1016/j.identj.2025.100873","url":null,"abstract":"<div><h3>Objective</h3><div>To evaluate the biomechanical behavior of 5 types of commonly used implant/abutment connectors, using Finite Element Methods (FEM).</div></div><div><h3>Methods</h3><div>Five models of implant-abutment connections were designed using computer-aided design (CAD) software: Tri-channel (M1), Conical internal hexagon (M2), Morse taper with an integrated screw (M3), Internal hexagon (M4), and Tube-in-tube (M5). The bone was modeled as coaxial cylinders, with the inner cylinder representing spongy bone and the outer 1 mm-thick cylinder representing cortical bone. A premolar crown geometry was designed onto the abutment with a 40 µm-thick cement layer. Three loading scenarios were applied to each model: (1) a 100 N compressive load, (2) a 50 N oblique load at 45° (relative to the implant axis), and (3) a 50 N lateral load.</div></div><div><h3>Results</h3><div>All stress and deformation values remain within the tolerable limits for the materials used. Notably, M1, M4, and M5 exhibited optimal biomechanical performance. M1 and M4 recorded the lowest stresses in implant components, 24.4 to 24.8 MPa in the abutment and 27.5 to 27.9 MPa in the screw under compression, along with minimal crown deformation (8.6 µm compared to M3’s 11.7 µm). In contrast, M3 experienced the highest implant-component stresses (68.5 MPa in the abutment and 120.2 MPa in the screw) but showed the lowest cortical bone stress at 7.7 MPa, versus 10.2 MPa in M4.</div></div><div><h3>Conclusion</h3><div>For long-term durability, implants with an internal hexagon (M4) or tri-channel (M1) design are preferable, as they minimize stress and deformation within the implant complex, thereby reducing the risk of prosthetic failure. While the Morse taper (M3) design may benefit patients with compromised bone density, its higher implant-component stresses warrant caution.</div></div><div><h3>Clinical significance</h3><div>This study provides valuable insights to support evidence-based selection of implant–abutment connection designs. Among the 5 evaluated systems, the Tri-channel (M1) and Internal Hexagon (M4) designs demonstrated superior biomechanical performance by minimizing stress concentrations within the implant components and surrounding bone. These configurations are therefore recommended for routine clinical use to enhance prosthetic stability, reduce the likelihood of mechanical complications such as screw loosening or fracture, and prolong implant longevity. Conversely, although the Morse Taper with integrated screw (M3) design showed the lowest stress on cortical bone – suggesting potential benefit for patients with reduced bone quality – it exhibited the highest stress levels within implant components, indicating a higher mechanical failure risk. Clinicians should weigh these biomechanical trade-offs when planning treatment, particularly in patients with high functional loads or compromised bone conditions.</div></div>","PeriodicalId":13785,"journal":{"name":"International dental journal","volume":"75 4","pages":"Article 100873"},"PeriodicalIF":3.2,"publicationDate":"2025-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144480205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Involvement of the Programmed Death 1/Programmed Death Ligand 1 Pathway in the Immune Microenvironment of Chronic Periapical Lesions","authors":"Yujin Song ,&nbsp;Seohee Park ,&nbsp;Hyeseong Jin ,&nbsp;Byungyoon Hyun ,&nbsp;Kyu-Young Oh","doi":"10.1016/j.identj.2025.100874","DOIUrl":"10.1016/j.identj.2025.100874","url":null,"abstract":"<div><h3>Introduction and aims</h3><div>This study aimed to determine the pathogenic role and clinicopathological significance of the immune microenvironment including the programmed death 1 (PD-1)/programmed death ligand 1 (PD-L1) pathway in chronic periapical lesions.</div></div><div><h3>Methods</h3><div>A total of 20 chronic periapical lesions consisting of 10 periapical granulomas and 10 periapical cysts were included in this study. Immunohistochemistry was performed for immune cell populations, including PD-L1, PD-1, CD4, CD8, FOXP3, and CD20. Immune cell populations were quantitatively evaluated on digitized slides. The associations between each immune cell population and clinicopathological factors and between immune cell populations were statistically analysed.</div></div><div><h3>Results</h3><div>Lesion size was positively associated with the density of PD-L1+ macrophages (<em>P</em> &lt; .001, Fisher exact test; <em>r</em> = 0.455, <em>P</em> = .044, Pearson correlation analysis) and CD8+ cytotoxic T cells (<em>P</em> = .020, Fisher exact test; <em>r</em> = 0.471, <em>P</em> = .036, Pearson correlation analysis). No associations were found between immune cell populations and other clinicopathological factors, including age, sex, lesion location, and diagnosis. A moderate positive correlation was observed between the density of PD-L1+ macrophages and CD8+ cytotoxic T cells (<em>r</em> = 0.537, <em>P</em> = .015). The density of PD-1+ cells showed a strong positive correlation with the density of CD4+ helper T cells (<em>r</em> = 0.719, <em>P</em> &lt; .001) and FOXP3+ regulatory T cells (<em>r</em> = 0.784, <em>P</em> &lt; .001).</div></div><div><h3>Conclusion</h3><div>These findings suggest that cytotoxic T cells are implicated in the progression of chronic periapical lesions, which may be regulated by PD-L1+ macrophages. PD-1 may be involved in helper T cell exhaustion and regulatory T cell activity in chronic periapical lesions.</div></div><div><h3>Clinical relevance</h3><div>We demonstrated the involvement of PD-L1 and PD-1 in the regulation of T cell immunity in chronic periapical lesions. These findings suggest that activating the PD-1/PD-L1 pathway is a potential therapeutic strategy for chronic periapical lesions.</div></div>","PeriodicalId":13785,"journal":{"name":"International dental journal","volume":"75 4","pages":"Article 100874"},"PeriodicalIF":3.2,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144471121","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of a Decellularisation Process for Developing an Osteogenic Periosteum Membrane In Vitro","authors":"Nabiha B. Remmani ,&nbsp;K.G. Aghila Rani ,&nbsp;Rand Q. Alzaidy ,&nbsp;Abdulrahman S. Abdulrahman ,&nbsp;Shahd M. Darwish ,&nbsp;Melook A. Jawad ,&nbsp;A.R. Samsudin","doi":"10.1016/j.identj.2025.100869","DOIUrl":"10.1016/j.identj.2025.100869","url":null,"abstract":"<div><h3>Introduction</h3><div>The study addresses the need for a biocompatible, resorbable membrane with osteogenic properties, proposing a periosteum-based scaffold because of its inherent growth proteins and pliability.</div></div><div><h3>Methods</h3><div>The skin from the skull of a young sheep was removed to expose the connective tissue. A 2 cm x 2 cm periosteum sample was harvested and divided into 4 groups for different decellularisation treatments: sample A (control) was left untreated, sample B was treated with 100% alcohol, sample C was treated with 100% alcohol and 1% sodium hypochlorite, and sample D was treated with 100% alcohol, 1% sodium hypochlorite and 0.1% SDS. The processed periosteum was lyophillised and subjected to histological evaluation, scanning electron microscopy with energy-dispersive X-ray spectroscopy (SEM-EDS), RAMAN spectroscopy, cell attachment and viability studies. Statistical analysis was performed using GraphPad Prism.</div></div><div><h3>Results</h3><div>Group IV scaffold, treated with 100% alcohol, 1% sodium hypochlorite and 0.1% SDS, exhibited the most desirable characteristics for tissue engineering. SEM-EDS analysis revealed a clean, residue-free, well-structured collagen fibril network with significant porosity, while RAMAN spectroscopy confirmed preservation of collagen's triple-helical structure. Histological evaluation showed thorough decellularisation and the absence of cellular remnants. DAPI staining and SEM imaging demonstrated robust osteoblast attachment and proliferation over 24, 48 and 72 hours, suggesting its biomimetic property.</div></div><div><h3>Conclusion</h3><div>Decellularised ovine periosteum membrane from Group IV demonstrated biocompatibility and supported osteoblast growth, indicating its potential as a soft, osteogenic scaffold for repairing periodontal bone lesions. Further <em>in vivo</em> studies are recommended to confirm its effectiveness in treating periodontal defects for guided bone regeneration.</div></div><div><h3>Clinical relevance</h3><div>The optimised ovine periosteum scaffold demonstrates promising osteogenic properties and biocompatibility, making it a potential candidate for periodontal bone defect repair. Its resorbable nature and structural integrity offer advantages over synthetic membranes. This novel membrane aligns with the global goals of sustainable health care and waste reduction. Further <em>in vivo</em> validation is warranted before clinical application.</div></div>","PeriodicalId":13785,"journal":{"name":"International dental journal","volume":"75 4","pages":"Article 100869"},"PeriodicalIF":3.2,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144471122","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Long Noncoding RNA MIR4435-2HG is Involved in Osteogenic Differentiation and Inflammation in Human Dental Pulp Stem Cells","authors":"Feijun Wu ,&nbsp;Zhisheng Jiang","doi":"10.1016/j.identj.2025.100866","DOIUrl":"10.1016/j.identj.2025.100866","url":null,"abstract":"<div><h3>Introduction and aims</h3><div>Pulpitis is a common oral pathology, necessitating an investigation into its pathogenesis and therapeutic targets. The aim of this study was to elucidate the function of long noncoding RNA MIR4435-2 host gene (MIR4435-2HG) in osteogenic differentiation and inflammatory response of dental pulp cells in vitro, offering insights into pulpitis mechanisms and potential treatment strategies.</div></div><div><h3>Methods</h3><div>The expression of MIR4435-2HG was quantified by real-time quantitative PCR in pulp samples. Human dental pulp stem cells (hDPSCs) were utilized to investigate the impact of MIR4435-2HG on osteogenic differentiation. Inflammatory responses were induced by treating hDPSCs with lipopolysaccharide (LPS). The viability and apoptosis of hDPSCs were determined using Cell Counting Kit-8 and Apoptosis assays, respectively. The targeting relationship between MIR4435-2HG and miR-296-5p was substantiated using Dual-Luciferase reporter gene assay.</div></div><div><h3>Results</h3><div>MIR4435-2HG was upregulated in the inflamed pulp tissues and exhibited favourable efficacy in differentiating between healthy and inflamed pulp. In osteogenesis-induced hDPSCs, MIR4435-2HG suppressed alkaline phosphatase activity and the expression of osteogenic markers (dentin matrix protein-1 and dentin salivary phosphoprotein). In LPS-stimulated hDPSCs, knockdown of MIR4435-2HG decreased the mRNA levels of inflammatory mediators (TNF-α, IL-1β, and IL-6), promoted cell proliferation, and repressed apoptosis. miR-296-5p was negatively modulated by MIR4435-2HG, and downregulation of miR-296-5p counteracted the effects of MIR4435-2HG knockdown on inflammation and cellular phenotype.</div></div><div><h3>Conclusion</h3><div>The present investigation indicates that MIR4435-2HG suppresses osteogenic differentiation while facilitating LPS-triggered inflammation in hDPSCs through miR-296-5p modulation.</div></div><div><h3>Clinical relevance</h3><div>This study investigated how MIR4435-2HG regulates osteogenic differentiation in hDPSCs and modulates LPS-induced inflammatory reactions. The findings shed light on its possible involvement in pulpitis pathogenesis and suggest its therapeutic potential for dental tissue repair.</div></div>","PeriodicalId":13785,"journal":{"name":"International dental journal","volume":"75 4","pages":"Article 100866"},"PeriodicalIF":3.2,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144471123","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Novel KDF1 Variant is Associated With Multiple Natal Teeth, Tooth Agenesis, and Root Maldevelopment","authors":"John M. Graham Jr. ,&nbsp;Pedro A. Sanchez-Lara ,&nbsp;Atsushi Ohazama ,&nbsp;Katsushige Kawasaki ,&nbsp;Stefan T. Arold ,&nbsp;Piranit Nik Kantaputra","doi":"10.1016/j.identj.2025.100860","DOIUrl":"10.1016/j.identj.2025.100860","url":null,"abstract":"<div><h3>Objective</h3><div>Natal teeth are teeth that are present at birth. Multiple natal teeth are extremely rare. The objective of this study was to find the molecular aetiology of a unique dental phenotype including natal teeth, tooth agenesis, and root maldevelopment in a 5-generation family.</div></div><div><h3>Methods</h3><div>Oral and radiographic examination, linkage analysis, whole genome sequencing, and an immunohistochemical study of Kdf1 during tooth development in the mouse embryo were performed. A protein model was generated.</div></div><div><h3>Results</h3><div>We report a 5-generation family in which multiple natal teeth, oligodontia, and root maldevelopment manifested with autosomal dominant inheritance. Linkage analysis and whole genome sequencing revealed a novel pathogenic variant c.845T&gt;G; p.Ile282Ser, which cosegregated in 9 affected and 10 unaffected family members. This amino acid Ile282 is highly conserved and is important for the stabilization of a small helical fragment. This stabilization is lost in the Ile282Ser mutant, resulting in disruption of the interaction of KDF1 with its partner proteins, including IKKA, which are important for epidermal proliferation and differentiation and subsequent tooth development.</div></div><div><h3>Conclusions</h3><div>Our study demonstrates for the first time that natal teeth, tooth agenesis, and root maldevelopment are caused by a <em>KDF1</em> variant. Our study highlights the important role of <em>KDF1</em> in tooth formation and eruption.</div></div>","PeriodicalId":13785,"journal":{"name":"International dental journal","volume":"75 4","pages":"Article 100860"},"PeriodicalIF":3.2,"publicationDate":"2025-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144365035","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"SOX11 and GLIS2: Novel Biomarkers for Understanding the Progression of Oral Leukoplakia","authors":"Yi Li ,&nbsp;Zhencun Tang ,&nbsp;Chenliang Xiao ,&nbsp;Jun Su ,&nbsp;Lijia He ,&nbsp;Wenyun Zhang","doi":"10.1016/j.identj.2025.100872","DOIUrl":"10.1016/j.identj.2025.100872","url":null,"abstract":"<div><h3>Background</h3><div>Oral leukoplakia (LP) is a common precancerous lesion that can progress to oral squamous cell carcinoma (OSCC). Although various risk factors have been identified, the molecular mechanisms and biomarkers of LP remain insufficiently understood.</div></div><div><h3>Methods</h3><div>This study integrated single-cell RNA sequencing with bulk RNA sequencing data from leukoplakia tissues to investigate the transcriptional regulatory mechanisms within LPtissues. Our aim is to identify key molecular markers and their associated pathways. Furthermore, by analyzing survival data from OSCC in the The Cancer Genome Atlas(TCGA) database, we assessed the prognostic significance of these molecular markers in tumors. Finally, we validated the potential roles of relevant molecular markers in the progression of leukoplakia using patient LP tissue samples.</div></div><div><h3>Results</h3><div>The analysis revealed that single-cell RNA sequencing data indicated that fibroblasts in LP tissues possess a unique gene regulatory network, exhibiting significantly different transcriptional characteristics compared to normal tissues. We identified SOX11 and GLIS2 as LP-specific molecular markers, with their expression significantly elevated in LP tissues compared to normal tissues. Transcriptomic profiling of LP reveals that SOX11 and GLIS2 positively correlate with the activation of epithelial-mesenchymal transition (EMT) and angiogenesis. Additionally, analysis of OSCC samples demonstrated that high expression levels of SOX11 and GLIS2 were associated with poor prognosis. Immunohistochemical staining further showed a gradual increase in the expression of SOX11 and GLIS2 during the progression of leukoplakia, supporting their potential application as biomarkers.</div></div><div><h3>Conclusion</h3><div>As key transcription factors in LP, SOX11 and GLIS2 may influence the occurrence and development of lesions by regulating EMT and angiogenesis biological processes. They have the potential to serve as biomarkers and therapeutic targets for LP and OSCC, providing new insights into understanding the mechanisms of LP and developing treatment strategies.</div></div>","PeriodicalId":13785,"journal":{"name":"International dental journal","volume":"75 4","pages":"Article 100872"},"PeriodicalIF":3.2,"publicationDate":"2025-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144329724","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Do 3D-Printed and Milled Denture Bases Differ in Microbial Activity and Adhesion? A Systematic Review and Meta-Analysis","authors":"Sarah Arzani ,&nbsp;Erfan Khorasani ,&nbsp;Aida Mokhlesi ,&nbsp;Shima Azadian ,&nbsp;Safoura Ghodsi ,&nbsp;Seyed Ali Mosaddad","doi":"10.1016/j.identj.2025.100857","DOIUrl":"10.1016/j.identj.2025.100857","url":null,"abstract":"<div><div>The increasing adoption of digital manufacturing in prosthodontics raises concerns about microbial metabolic activity and adhesion on denture bases fabricated via additive and subtractive methods. This systematic review aimed to compare microbial metabolic activity and microorganism adhesion between 3D-printed and milled complete denture bases. A systematic search of PubMed, Scopus, Web of Science, Embase, and the Cochrane Library was conducted up to December 2024. Studies were screened according to predefined eligibility criteria, and data were independently extracted by two reviewers. The standardized mean difference (SMD) was used to compare microbial metabolic activity and cell adhesion outcomes between groups. A random-effects model was applied for meta-analysis, and heterogeneity was assessed using Cochran's Q, I², and τ² indices. Publication bias was evaluated using funnel plots, Egger's test, and the Trim and Fill method. The Quality Assessment Tool for In Vitro Studies (QUIN) was used to assess the risk of bias. Twelve studies met the inclusion criteria. The meta-analysis revealed that microbial activity was significantly higher in 3D-printed compared to milled denture bases (SMD = 1.285, 95% CI: 0.810-1.760, <em>P</em> &lt; .001). Similarly, the microbial cell count was significantly higher in the 3D-printed group (SMD = 1.050, 95% CI: 0.124-1.976, <em>P =</em> .026). Surface roughness was tested as a covariate in meta-regression and was not a significant predictor of microbial activity. 3D-printed denture bases exhibited higher microbial metabolic activity and adhesion than milled counterparts. However, the heterogeneity of studies and variations in fabrication parameters necessitate further research to optimize material selection and processing protocols.</div></div>","PeriodicalId":13785,"journal":{"name":"International dental journal","volume":"75 4","pages":"Article 100857"},"PeriodicalIF":3.2,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144312709","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}