International Journal of Biosciences最新文献

{"title":"CVPDr DNA FRAGMENT AFFECT DIFFERENCES IN RESISTANT TO CITRUS VEIN PHLOEM DEGENERATION (CVPD) DISEASE, NUTRIENT DEFICIENCIES AND QUALITY OF FRUITS","authors":"I. G. A. D. Yuniti, I. Wirawan, I. Wijaya, M. Sritamin","doi":"10.24843/ijbb.2017.v05.i01.p05","DOIUrl":"https://doi.org/10.24843/ijbb.2017.v05.i01.p05","url":null,"abstract":"Citrus Vein Phloem Degeneration (CVPD) disease is a major obstacle in the effort to develop and increase the production of citrus fruits in Bali. The study on the polymorphism of CVPD r DNA fragment shows that the CVPD r DNA fragment is resistant factor againt CVPD disease. This study try to elaborate the difference in resistance led to differences in plant nutrients deficiencies in the citrus plant with CVPD disease. . Besides, there are also difference in the quality of fruit due to CVPD disease attacks such as water content, vitamin C content and antioxidants in citrus fruits, color, flavor, taste and texture and fruit into small, hard and sour taste.","PeriodicalId":13776,"journal":{"name":"International Journal of Biosciences","volume":"1 1","pages":"60"},"PeriodicalIF":0.0,"publicationDate":"2017-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85242279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"POTENTIAL EFFECT OF MACRO ALGA Caulerpa sp. AND Gracilaria sp. EXTRACT LOWERING MALONDIALDEHYDE LEVEL OF WISTAR RATS FED HIGH CHOLESTEROL DIET","authors":"K. S. M. Julyasih, I. Wirawan","doi":"10.24843/IJBB.2017.V05.I01.P06","DOIUrl":"https://doi.org/10.24843/IJBB.2017.V05.I01.P06","url":null,"abstract":"Seaweed has potential nutrient content such as carotenoids, vitamins, fatty acids, carbohydrates, minerals, and other essential substances. Carotenoids have important biological functions as an antioxidant, and immunostimulatory which can prevent the disease, anti-inflammatory, anti-stress, anti-aging, and protect the skin from the harmful effects of ultraviolet radiation. Seaweed generally consumed as a vegetable by people in Bali, known as the local name Bulung Boni (Caulerpa spp.) and Bulung Sangu (Gracilaria spp.).. So far there has been no report or results of research on the effects of extract ethanol of Bulung Boni (Caulerpa sp.) and Bulung Sangu (Gracilaria sp.) as an antioxidant that can prevent lipid peroxidation which can be seen in decreased level of MDA in liver tissue or blood plasma. Therefore it is necessary to determine of plasmaMDA level of Wistar rat after fed high cholesterol diet treated with extract ethanol of Caulerpa sp. and Gracillaria sp. This experimental study used completely randomized design. Research using total of 24 Wistar rats divided into six sample groups of equal size, all fed with a diet high in cholesterol especially in negative control. The study consisted of negative control group (standard diet), positive control group (high cholesterol diet), high-cholesterol diet with Caulerpa sp. extract dose of 20 mg and 60 mg/100 g, high cholesterol diet with Gracilaria sp. extract dose of 20 mg and 60 mg/100 g body weight rat per day.The study resulted that rats fed high cholesterol diet with treated extract ethanol Caulerpa sp. and Gracilaria sp. with a dose of 20 mg and 60 mg per 100 g body weight rat / day had plasma MDA level significantly lower (p <0.05) compared with rats fed high cholesterol diet without treated with extract of Caulerpa sp. and Gracilaria sp.","PeriodicalId":13776,"journal":{"name":"International Journal of Biosciences","volume":"118 1","pages":"71"},"PeriodicalIF":0.0,"publicationDate":"2017-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82282553","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MORPHOLOGY, PHYSIOLOGY AND MOLECULAR CHARACTERISTICS OF OIL PALM (Elaeis guineensis Jacq.) ENDOPHYTIC Bacillus sp.","authors":"F. Puspita, Hadiwiyono Hadiwiyono, S. Poromarto, D. I. Roslim","doi":"10.24843/ijbb.2017.v05.i01.p07","DOIUrl":"https://doi.org/10.24843/ijbb.2017.v05.i01.p07","url":null,"abstract":"Endophytic bacteria are the bacteria that live in plant tissues. In oil palm tissue there are many types of endophytic bacteria and have a role that can be beneficial for the plant, one of them is endophytic Bacillus sp. The aim of these research was to obtain morphology, physiology and molecular characteristics of endophytic Bacillus sp. originating from oil palm tissue. Sampling was done by random simple sampling method. Isolation of bacteria was performed on plant tissues such as roots, midribs, stems and leaves of oil palm plants. The results of morphological characterization such as colony color, colony shape and colony edge show similarity in each isolate but there are differences in the surface morphology of the colony, where there are 6 isolates with convex surface and 6 isolates with flat shape. Physiological test results such as catalase test, oxidase test, starch hydrolysis test, motility test and temperature effect test on bacterial growth showed positive results in each isolate. Molecular characterization using 16S rRNA primers based on BLASTn shows that all isolates tested have similarities with Bacillus sp. Based on the phylogenetic tree it was found that the endophytic bacteria of Ba-B2 isolates were associated with Bacillus flexus with 100% consistency index grouped at a distance of 0.03 and Ba-P2 isolates were related to Bacillus substilis at a distance of 0.01 with an 89% consistency index.","PeriodicalId":13776,"journal":{"name":"International Journal of Biosciences","volume":"61 1","pages":"80"},"PeriodicalIF":0.0,"publicationDate":"2017-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77804773","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MINERAL FERTLIZER AS AN ALTERNATIVE FERTILIZER IN INCREASING RICE YIELD IN TABANAN REGENCY","authors":"I. Lanya, N. Subadiyasa","doi":"10.24843/ijbb.2017.v04.i02.p04","DOIUrl":"https://doi.org/10.24843/ijbb.2017.v04.i02.p04","url":null,"abstract":"Production can be increased through improved harvest index genetic engineering, and the availability of nutrients in the soil. Ciherang rice production tests conducted over two years by toposekuen in Tabanan. First in five Subak, (Jangga, Bulung Daya, Andal Dewa, Lanyah1, and Perean) with 10 fertilizer treatments. The second year, five fertilization treatments in the two Subak (Petung dan Babahan). The study was conducted in randomized complete block design with three replications. Parameters observed included vegetative and generative growth, and yield of paddy field farming. Duncan`s test, with a confidence level of 5% using the Costat program, and input output fertilization. Fertilization is very significant effect on rice production. The first year results, the highest rice production (9.33 tons ha-1) was achieved in a combination of fertilizer treatment (organic + NPK + mineral), can increase production 43.98%. The second year of the highest rice production (11.878 tons ha) were achieved on the fertilization of NPM (100 kg urea + 100 kg Phonska ha) + organic fertilizer (2.5 tons ha) + mineral fertilizer (2.5 tons ha), production increased 50.4%. Organic fertilization received the lowest rice production (5,420 to 8,940 tons ha), and the highest percentage of empty grain (9.29%). Mineral fertilizers can reduce the use of chemical fertilizers and high-dose organic fertilizers. The increase in yield of paddy field farming compared to control for the treatment of organic fertilizer (Rp 1.011 million ha), NP (Rp 6.416 million ha), NPK (Rp7.636 million ha), and NPM (Rp12.576 million ha ).","PeriodicalId":13776,"journal":{"name":"International Journal of Biosciences","volume":"19 1","pages":"99"},"PeriodicalIF":0.0,"publicationDate":"2017-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84757525","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"THE QUALITY AND TOTAL MICROBES OF BROILER CHICKEN IN TRADITIONAL MARKETS OF DENPASAR CITY","authors":"Luh Yuni Surya Antari, I. Ariana, G. Dewi","doi":"10.24843/IJBB.2017.V04.I02.P03","DOIUrl":"https://doi.org/10.24843/IJBB.2017.V04.I02.P03","url":null,"abstract":"The purpose of this study was to determine the quality and total microbes of broiler at different location in the traditional markets of Denpasar City. This study used a completely randomized design (CRD) with four treatments. The treatments used in this study, broiler chicken sold at traditional markets in East (DT), North (DU), West (DB), and South of Denpasar (DS). The data obtained from this study were analyzed by analysis of variance. If there were significant differences (P <0,05) among the treatments, then continued with Duncan's multiple range test. The variables used in this study were carcass weight, cooking loss, drip loss, water holding capacity, water content, protein, fat, ash, and total microbes. The results showed that on the average, the highest carcass weight in DB treatment was 1.482 grams, with other treatments to get results not significantly different (P>0,05). The result of this study for variable drip loss, water content, protein, fat, and ash significantly different (P<0,05), then research result for cooking loss, and water holding capacity, were not significantly different (P>0,05). The research result for total microbies broiler chicken at traditional markets in Denpasar City have a range of 1,84 x 10 4 to 2,62 x 10 4 CFU /gram. The results were still normal when compared to SNI 7388: 2009 which states that the maximum amount that the content of TPC 1x10 6 CFU/gram. From these results, it was concluded that there were similarities quality of broiler chicken (carcass weight, cooking loss, water holding capacity, and total microbes), however, there were differences in the quality of broiler seen from drip loss, water content, protein, fat, and ash of broiler at traditional markets in Denpasar City.","PeriodicalId":13776,"journal":{"name":"International Journal of Biosciences","volume":"43 1","pages":"92"},"PeriodicalIF":0.0,"publicationDate":"2017-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79227163","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ISOLATION AND IDENTIFICATION OF Pseudomonas spp. TO CONTROL Plasmodiophora brassciae, THE PATHOGEN OF CLUBROOT DISEASE ON CABBAGE","authors":"Ketut Suada, A. Suwastika","doi":"10.24843/IJBB.2017.V04.I02.P01","DOIUrl":"https://doi.org/10.24843/IJBB.2017.V04.I02.P01","url":null,"abstract":"Clubroot is very detrimental disease to cabbage production so as farmers work on various efforts to control it. The use of fungicides not only ineffective but also pollute the environment, therefore biological control system need to be pursued. The use of antagonistic agents such as Pseudomonas has been widely studied and known effective in suppressing various pathogens. Therefore it is worth trying its effectiveness against Plasmodiophora brassicae, a pathogen of cabbage. The purpose of this study was to obtain indigenous Pseudomonas which effectively suppress the pathogens and may also increase plant growth. Microbes were isolated from the cabbage area using the Kings'B medium with multilevel dilution. All isolates were tested for their effectiveness in pots in a Completely Randomized Design with a concentration of 1.5x106 CFU (Colony Forming Unit) per pot. The variables observed were plant growth, number of club roots, and percentage of disease incidence. Fourteen isolates of Pseudomonas were isolated. Three Pseudomonas isolates were found most effective at suppressing clubroot disease and increasing plant growth. The best isolate obtained was Pseudomonas-6, followed Pseudomonas-9, and Pseudomonas-8.","PeriodicalId":13776,"journal":{"name":"International Journal of Biosciences","volume":"8 1","pages":"75"},"PeriodicalIF":0.0,"publicationDate":"2017-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73112116","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CROP IMPROVEMENT THROUGH INDUCING MUTAGENESIS IN VIVO USING COLCHICINE ON COWPEA (Vigna unguiculata L. Walp)","authors":"I. G. Susrama, I. Wirawan","doi":"10.24843/IJBB.2017.V04.I02.P02","DOIUrl":"https://doi.org/10.24843/IJBB.2017.V04.I02.P02","url":null,"abstract":"Colchicine treatments of 0.005% and 0.01% either in distilled water or in hydrogen peroxide 3% were caused temporary leaf shape changes on cowpea. Treatment of colchicine in hydrogen peroxide 3% resulted in green pod color change to purple however treatment of just hydrogen peroxide 3% and colchicine in hydrogen peroxide 3% caused 8.8-10.7% dwarf cowpea. As a preliminary data, we also observed that cowpea crops grown from seeds treated with colchicine in distillate water attacked by bean leaf beetle much earlier compare to the treatment with colchicine in hydrogen peroxide 3%.","PeriodicalId":13776,"journal":{"name":"International Journal of Biosciences","volume":"107 ","pages":"85"},"PeriodicalIF":0.0,"publicationDate":"2017-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91451326","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"HEXOKINASES IN BREAST CANCER","authors":"S. L. Kharitonov, A. Zikiriahodzhaev, M. Ermoshchenkova, A. Sukhot’ko, M. Fedorova, E. Pudova, B. Alekseev, A. Kaprin, A. Kudryavtseva","doi":"10.24843/ijbb.2017.v04.i02.p05","DOIUrl":"https://doi.org/10.24843/ijbb.2017.v04.i02.p05","url":null,"abstract":"","PeriodicalId":13776,"journal":{"name":"International Journal of Biosciences","volume":"42 1","pages":"110"},"PeriodicalIF":0.0,"publicationDate":"2017-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83102183","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of exopolysaccharide producing LAB isolated from Zoom-koom, a cereal-based traditional beverage from Burkina Faso","authors":"Fidèle Wend-bénédo Tapsoba, H. Sawadogo-Lingani, R. Coda, D. Compaoré, Sérémé, K. Katina, Donatien Kaboré, Hadou Haro, M. Dicko, N. Maina","doi":"10.12692/ijb/11.6.45-60","DOIUrl":"https://doi.org/10.12692/ijb/11.6.45-60","url":null,"abstract":"Zoom-koom is a traditional fermented beverage from Burkina Faso produced from millet or sorghum grains. A total of 33 Lactic acid bacteria (LAB) that produce exopolysaccharides (EPS) were isolated from zoom-koomand characterized in order to select the most efficient as starter cultures to improve the quality of zoom-koom. Physiological and morphological characteristics were determined using growth tests and microscopic observation, respectively. Activities of starch degrading enzymes (amylases) were performed using biochemical methods. Antimicrobial activities were determined using disc diffusion assay and overlay method. Genotypic characterization and identification of selected LAB EPS synthetizing isolates which were able to produce antimicrobial compound were performed through 16S rRNA sequencing. EPSs Structures of selected LAB isolates were determined using NMR spectroscopy. Among LAB isolates 76% was hetero-fermentative while the other was homo-fermentative. Some isolates (15%) could grow in the presence of bile and esculin and 12% were able to grow on MRS-starch. Although all the isolates were able to produce EPSs, only 6% were efficient producers. Among the screened LAB isolates, 7had inhibitory activities against Escherichiacoli81 nr.149 SKN 541, Pseudomonas aeruginosa ATCC and Salmonella typhimurium O:1036340P/t49. In addition 6 isolates displayed antifungal activities against Aspergillus fumugatus, Aspergillus flavus and Aspergillus niger. The 16S rRNA sequencing showed that most of the isolates were clustered with Weissella cibaria/confusa and the others were clustered with Lactobacillus plantarum/pentosus and Lactococcus lactis/garvieae. The NMR spectroscopy showed that excreted EPSs are ramified glucanpolyaccharides containing α-(13) and α-(16) linkages, where the later are the main chains. * Corresponding Author: Fidèle Wend-bénédo Tapsoba  tapfidelew@gmail.com International Journal of Biosciences | IJB | ISSN: 2220-6655 (Print), 2222-5234 (Online) http://www.innspub.net Vol. 11, No. 6, p. 45-60, 2017","PeriodicalId":13776,"journal":{"name":"International Journal of Biosciences","volume":"128 1","pages":"45-60"},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89208374","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Response of groundnut to plant density and phosphorous application in the Sudan savanna zone of Nigeria","authors":"H. Ajeigbe, A. Kamara, A. Kunihya, A. H. Inuwa, A. Adinoyi","doi":"10.12692/IJB/9.1.291302","DOIUrl":"https://doi.org/10.12692/IJB/9.1.291302","url":null,"abstract":"Despite the recent release of several improved varieties of groundnut in Nigeria the productivities have not \u0000increase significantly due to lack of commensurate recommendation in agronomic practices. Two groundnut \u0000varieties were evaluated for their response to different plant density and phosphorus application in two locations \u0000in the Sudan Savanna zone of Nigeria in 2012 and 2013. The groundnut were planted at density of 44444, 66667, \u0000and 133333 hills ha-1 with average of two plants per hill. Phosphorus was applied at rate of 0 or 20 kg P ha-1. P \u0000fertilizer application increased pod and haulm yields by 26% and 16% respectively in Minjibir. It increased pod \u0000and haulm yields by 62% and 27% respectively in Wudil. Pod and haulm yields, harvest index, revenue, profit \u0000and cost benefit ratio increased with increasing plant density. Samnut-24 produced pod yields that were \u0000significantly higher than Samnut-22 across treatments. Pod yields at density of 133,333 hills ha-1 was 31% higher \u0000than at 66667 and 40% than at 44,444 hills ha-1. Application of fertilizer increased profit by 22% and 49% in \u0000Minjibir and Wudil respectively. Planting at density of 133,333 hill ha-1 increased profit by 19% and 27% over \u000066,667 and 444444 hill ha-1 respectively in Minjibir, while it increase profit by 9% in Wudil. Cultivation of \u0000Samnut-24 at high density with phosphorus application will make groundnut production a more profitable \u0000venture in Sudan Savanna zone of Nigeria.","PeriodicalId":13776,"journal":{"name":"International Journal of Biosciences","volume":"127 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2016-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78022100","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}