Characterization of exopolysaccharide producing LAB isolated from Zoom-koom, a cereal-based traditional beverage from Burkina Faso

International Journal of Biosciences Pub Date : 2017-01-01 DOI:10.12692/ijb/11.6.45-60

Fidèle Wend-bénédo Tapsoba, H. Sawadogo-Lingani, R. Coda, D. Compaoré, Sérémé, K. Katina, Donatien Kaboré, Hadou Haro, M. Dicko, N. Maina

{"title":"Characterization of exopolysaccharide producing LAB isolated from Zoom-koom, a cereal-based traditional beverage from Burkina Faso","authors":"Fidèle Wend-bénédo Tapsoba, H. Sawadogo-Lingani, R. Coda, D. Compaoré, Sérémé, K. Katina, Donatien Kaboré, Hadou Haro, M. Dicko, N. Maina","doi":"10.12692/ijb/11.6.45-60","DOIUrl":null,"url":null,"abstract":"Zoom-koom is a traditional fermented beverage from Burkina Faso produced from millet or sorghum grains. A total of 33 Lactic acid bacteria (LAB) that produce exopolysaccharides (EPS) were isolated from zoom-koomand characterized in order to select the most efficient as starter cultures to improve the quality of zoom-koom. Physiological and morphological characteristics were determined using growth tests and microscopic observation, respectively. Activities of starch degrading enzymes (amylases) were performed using biochemical methods. Antimicrobial activities were determined using disc diffusion assay and overlay method. Genotypic characterization and identification of selected LAB EPS synthetizing isolates which were able to produce antimicrobial compound were performed through 16S rRNA sequencing. EPSs Structures of selected LAB isolates were determined using NMR spectroscopy. Among LAB isolates 76% was hetero-fermentative while the other was homo-fermentative. Some isolates (15%) could grow in the presence of bile and esculin and 12% were able to grow on MRS-starch. Although all the isolates were able to produce EPSs, only 6% were efficient producers. Among the screened LAB isolates, 7had inhibitory activities against Escherichiacoli81 nr.149 SKN 541, Pseudomonas aeruginosa ATCC and Salmonella typhimurium O:1036340P/t49. In addition 6 isolates displayed antifungal activities against Aspergillus fumugatus, Aspergillus flavus and Aspergillus niger. The 16S rRNA sequencing showed that most of the isolates were clustered with Weissella cibaria/confusa and the others were clustered with Lactobacillus plantarum/pentosus and Lactococcus lactis/garvieae. The NMR spectroscopy showed that excreted EPSs are ramified glucanpolyaccharides containing α-(13) and α-(16) linkages, where the later are the main chains. * Corresponding Author: Fidèle Wend-bénédo Tapsoba  tapfidelew@gmail.com International Journal of Biosciences | IJB | ISSN: 2220-6655 (Print), 2222-5234 (Online) http://www.innspub.net Vol. 11, No. 6, p. 45-60, 2017","PeriodicalId":13776,"journal":{"name":"International Journal of Biosciences","volume":"128 1","pages":"45-60"},"PeriodicalIF":0.0000,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"4","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Journal of Biosciences","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.12692/ijb/11.6.45-60","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}

引用次数: 4

Abstract

Zoom-koom is a traditional fermented beverage from Burkina Faso produced from millet or sorghum grains. A total of 33 Lactic acid bacteria (LAB) that produce exopolysaccharides (EPS) were isolated from zoom-koomand characterized in order to select the most efficient as starter cultures to improve the quality of zoom-koom. Physiological and morphological characteristics were determined using growth tests and microscopic observation, respectively. Activities of starch degrading enzymes (amylases) were performed using biochemical methods. Antimicrobial activities were determined using disc diffusion assay and overlay method. Genotypic characterization and identification of selected LAB EPS synthetizing isolates which were able to produce antimicrobial compound were performed through 16S rRNA sequencing. EPSs Structures of selected LAB isolates were determined using NMR spectroscopy. Among LAB isolates 76% was hetero-fermentative while the other was homo-fermentative. Some isolates (15%) could grow in the presence of bile and esculin and 12% were able to grow on MRS-starch. Although all the isolates were able to produce EPSs, only 6% were efficient producers. Among the screened LAB isolates, 7had inhibitory activities against Escherichiacoli81 nr.149 SKN 541, Pseudomonas aeruginosa ATCC and Salmonella typhimurium O:1036340P/t49. In addition 6 isolates displayed antifungal activities against Aspergillus fumugatus, Aspergillus flavus and Aspergillus niger. The 16S rRNA sequencing showed that most of the isolates were clustered with Weissella cibaria/confusa and the others were clustered with Lactobacillus plantarum/pentosus and Lactococcus lactis/garvieae. The NMR spectroscopy showed that excreted EPSs are ramified glucanpolyaccharides containing α-(13) and α-(16) linkages, where the later are the main chains. * Corresponding Author: Fidèle Wend-bénédo Tapsoba  tapfidelew@gmail.com International Journal of Biosciences | IJB | ISSN: 2220-6655 (Print), 2222-5234 (Online) http://www.innspub.net Vol. 11, No. 6, p. 45-60, 2017

查看原文本刊更多论文

从布基纳法索的一种谷物饮料Zoom-koom中分离的产外多糖的鉴定

Zoom-koom是一种来自布基纳法索的传统发酵饮料，由小米或高粱谷物制成。摘要从变焦菇中分离得到33株产外多糖的乳酸菌，并对其进行了鉴定，以选择最有效的发酵剂，提高变焦菇的品质。通过生长试验和显微观察分别测定了生理和形态特征。采用生化法测定淀粉降解酶(淀粉酶)的活性。采用圆盘扩散法和覆盖法测定其抑菌活性。通过16S rRNA测序对合成的抗菌化合物进行基因型鉴定和鉴定。采用核磁共振光谱法测定分离菌株的eps结构。乳酸菌中76%为异发酵菌，其余为同发酵菌。一些分离株(15%)可以在胆汁和胰蛋白酶存在下生长，12%可以在mrs -淀粉上生长。虽然所有的分离株都能产生eps，但只有6%是有效的生产者。其中7株对大肠杆菌81 n .149 SKN 541、铜绿假单胞菌ATCC和鼠伤寒沙门菌O:1036340P/t49具有抑制活性。此外，6株分离物对烟曲霉、黄曲霉和黑曲霉表现出抗真菌活性。16S rRNA测序结果显示，大部分分离株与猪粪魏氏菌(Weissella cibaria/confusa)聚集在一起，其余与植物乳杆菌(Lactobacillus plantarum/pentosus)和乳酸乳球菌(Lactococcus lactis/garvieae)聚集在一起。核磁共振波谱分析表明，分泌的eps是含有α-(13)和α-(16)键的分支化葡聚糖，其中α-(16)键是主要链。*通讯作者:fid le wend - b 生物科学杂志/ IJB | ISSN: 2220-6655(印刷)，2222-5234(在线)http://www.innspub.net Vol. 11, No. 6, p. 45-60, 2017

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

International Journal of Biosciences

自引率

0.00%

发文量