International Journal of Biosciences最新文献_第7页

PRICK AND SOAK Agroacterium tumefaciens-MEDIATED IN PLANTA TRANSFORMATION IN TOMATO (Lycopersicon esculentum Mill.) 刺刺和浸渍农杆菌介导的番茄植株转化

International Journal of Biosciences Pub Date : 2018-05-21 DOI: 10.24843/IJBB.2018.V05.I02.P05

I. Sanjaya, R. Dwiyani, I. Wirawan, B. Sugiharto

{"title":"PRICK AND SOAK Agroacterium tumefaciens-MEDIATED IN PLANTA TRANSFORMATION IN TOMATO (Lycopersicon esculentum Mill.)","authors":"I. Sanjaya, R. Dwiyani, I. Wirawan, B. Sugiharto","doi":"10.24843/IJBB.2018.V05.I02.P05","DOIUrl":"https://doi.org/10.24843/IJBB.2018.V05.I02.P05","url":null,"abstract":"One of the modern plant breedings through genetic engineering is Agrobacterium tumefaciens-mediated transformation. Agrobacterium tumefaciens-mediated transformation can be performed in vitro or in planta. In planta transformation arises from the weaknesses of the in vitro method such as need high hygiene standard, professional tissue culture experts, and more time to prepare explants and somaclonal variation. In planta transformation is a method to transfer the gene to the plant genome without any tissue culture stages. The aims of this research were to know the possibility of the prick and soak in planta method with the target of tomato seeds and to know the most suitable inoculation time for tomato seeds transformation by prick and soak method the transformation is done by pricking the seeds and soaking them in the A. tumefaciens suspension. The treatments in this study were 1 and 2 days inoculation time to test the efficacy of prick and soak in planta transformation method. Tomato seeds were pricked with a needle on the center once, and then soaked in A. tumefaciens strain LB4404 suspension carrying pKYS-SoSPS1 plasmid with Neomycin Phosphotransferase (NPTII) and Saccharum officinarum Sucrose Phosphate synthase (SoSPS1) genes. Visualization of tomato’s DNA samples after PCR showed that 1-day inoculation sample was positively integrated with NPTII gene and negative in the 2 days inoculation treatment.","PeriodicalId":13776,"journal":{"name":"International Journal of Biosciences","volume":"97 1","pages":"124"},"PeriodicalIF":0.0,"publicationDate":"2018-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87227219","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

THE EFFECTS OF DIETARY INCLUSION OF DETOXIFIED Leucaena leucocephala LEAF MEAL ON THYROIDAL ACTIVITY OF RATS DURING GESTATION-LACTATION PERIOD 饲料中加入解毒银合欢叶粕对妊娠-哺乳期大鼠甲状腺功能的影响

International Journal of Biosciences Pub Date : 2018-05-21 DOI: 10.24843/ijbb.2018.v05.i02.p02

N. I. Wiratmini, I. Narayani, N. L. E. Setiasih

{"title":"THE EFFECTS OF DIETARY INCLUSION OF DETOXIFIED Leucaena leucocephala LEAF MEAL ON THYROIDAL ACTIVITY OF RATS DURING GESTATION-LACTATION PERIOD","authors":"N. I. Wiratmini, I. Narayani, N. L. E. Setiasih","doi":"10.24843/ijbb.2018.v05.i02.p02","DOIUrl":"https://doi.org/10.24843/ijbb.2018.v05.i02.p02","url":null,"abstract":"Leucaena leucocephala (L. leucocephala) provides the highest amount of proteins compared to other greens. However, its use is restricted due to the content of mimosine, an antinutrition non-protein amino acid known to be toxic to animals. This study aimed to observe the effect of administration of more than 10% of processed L. leucocephala leaf meal to the level of T3, T4 and the thyroid histopathology. In this study, L. leucocephala leaves were soaked in distilled water for 12 hours. The processed of L. leucocephala leaf meal was made into pellets containing 0%, 7.5%, 15%, and 22.5% leaf meal of total feeds respectively, and was fed to the rats during pregnancy and lactation. The level of triiodothyronine (T3) and thyroxine (T4), and the thyroid histological features were the parameters observed. The collected data were statistically analyzed by SPSS 20.0 for Windows using One-way ANOVA followed by Duncan Multiple Range Test to observe any significant difference among the 4 treatment groups (α=5%). Meanwhile, the presence of hyperplastic cells and follicle lumens filled with vesicles and colloids was descriptively analyzed by means of available literatures. The analysis of T3 and T4 level revealed that there was no any significant difference between the control and treatment groups. The epithelial cells of thyroid follicles in the treatment groups of P1, P2 and P3 showed hyperplasia and were detached from their respective basal membranes.","PeriodicalId":13776,"journal":{"name":"International Journal of Biosciences","volume":"84 1","pages":"103"},"PeriodicalIF":0.0,"publicationDate":"2018-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80429151","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

THE MOLECULAR DETECTION OF CITRUS VEIN PHLOEM DEGENERATION (CVPD) PATHOGEN (Liberobacter asiaticus) IN Diaphorina citri KUWAYAMA (HOMOPTERA : PSYLLIDAE) AND OTHER INSECTS ASSOCIATED WITH CITRUS PLANT 柑桔静脉韧皮部退化(CVPD)病原菌(亚洲自由杆菌)在柑桔类昆虫(同翅目:木虱科)中的分子检测

International Journal of Biosciences Pub Date : 2018-05-21 DOI: 10.24843/ijbb.2018.v05.i02.p07

I. Wijaya, Wayan Adiartayasa, M. Sritamin

引用次数: 0

ANALYSIS ON RELATIONSHIP AMONG DENDROBIUM spp BALI BASED ON CHARACTERISTICS OF LEAVES ANATOMY 基于叶片解剖特征的巴厘岛石斛亲缘关系分析

International Journal of Biosciences Pub Date : 2018-05-21 DOI: 10.24843/ijbb.2018.v05.i02.p03

I. A. P. Darmawati, I. N. Rai, R. Dwiyani, I. Astarini

{"title":"ANALYSIS ON RELATIONSHIP AMONG DENDROBIUM spp BALI BASED ON CHARACTERISTICS OF LEAVES ANATOMY","authors":"I. A. P. Darmawati, I. N. Rai, R. Dwiyani, I. Astarini","doi":"10.24843/ijbb.2018.v05.i02.p03","DOIUrl":"https://doi.org/10.24843/ijbb.2018.v05.i02.p03","url":null,"abstract":"From explorations in several parts of the forest of Bali, 24 species of Dendrobium orchids were identified. Relationship among these species needed to be mapped for further development of orchids. Characteristics of leaves anatomy have been used to determine relations among plants. Characteristics of anatomy that were observed in this study from the paradermal and transversal cut of the leaves were shape and number of epidermis cells, location of stomata, the direction of stomata opening, shape and number of stomata, length of the epidermis and stomata, density of stomata, index of stomata, and thickness of mesophyll. Analysis of relations among species was displayed as a dendogram, using the software Minitab 17 Vis. Analysis of relationship among 24 species of Dendrobium Bali based on the leaves anatomy used grouped average method in squared Euclidean distance with the scale of 0.349 to 0.972. At Euclidean distance of 0.349, two clusters were formed where D. aloifolium and D. subulatum were separated from the other clusters. At 80 % similarity coefficient, seven clusters were formed using similar method, with the 3 and 5 clusters had the shortest distance (169.35). If species that were clustered together were cross-bred, the success rate was higher; meanwhile, the further away the relation between species, the smaller the success rate of cross breeding. The possibility of getting high-quality genotypes was higher when the cross-breeding was successful.","PeriodicalId":13776,"journal":{"name":"International Journal of Biosciences","volume":"116 1","pages":"111"},"PeriodicalIF":0.0,"publicationDate":"2018-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81662670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

SPORE PROPAGATION OF INDIGENOUS ENDOMYCHORIZA FROM SEVERAL ROOTING AREAS OF SNAKE FRUIT ON DIFFERENT SOIL WATER CONTENT 不同土壤含水量下蛇果不同根区原生内生菌孢子繁殖的研究

International Journal of Biosciences Pub Date : 2018-05-21 DOI: 10.24843/ijbb.2018.v05.i02.p09

I. N. Rai, I. K. Suada, M. Praborini, I. W. Wiraatmaja

{"title":"SPORE PROPAGATION OF INDIGENOUS ENDOMYCHORIZA FROM SEVERAL ROOTING AREAS OF SNAKE FRUIT ON DIFFERENT SOIL WATER CONTENT","authors":"I. N. Rai, I. K. Suada, M. Praborini, I. W. Wiraatmaja","doi":"10.24843/ijbb.2018.v05.i02.p09","DOIUrl":"https://doi.org/10.24843/ijbb.2018.v05.i02.p09","url":null,"abstract":"Cultivation of organic snake fruit in Bali which is done on dry land with the irrigation depends on rainfall and the fertilization generally only uses uncertain amount of fallen leaves, it reduces the productivity, quality, and continuity of fruit production over time. In rhizosphere of snake fruit there are various types of indigenous endomycorrhiza that potentially can be developed as biofertilizer, but as a biofertilizer, the low number of spores population for inoculum becomes a limiting factor in using indigenous endomycorrhiza. The effort that can be done are to propagate the spores by giving water stress treatment. This study aimed to obtain the best rhizosphere location of snake fruit as the source of spores and the level of soil water content to multiply the spores. The research was conducted in the green House, Farm Station, Agriculture Faculty, Udayana University at Jalan Pulau Moyo, Denpasar, from October 2017 to January 2018. The spore propagation used nested experiment with Randomized Block Design patterns. The treatments were rhizosphere location as the source of indigenous endomycorrhizas spore consists of three levels (Bebandem District Karangasem Regency, Payangan District Gianyar Regency, and Pupuan District Tabanan Regency) and soil water content consists of three levels (100%, 70%, and 40% field capacity). The results of the research showed that the source of indigenous endomychorrizae from snake fruit rhizosphere in Payangan District gave the highest number of spores found and the highest percentage of the spore increase after propagation. While in the soil water content treatments, the highest number of spores found and the percentage of the spore increase after propagation were obtained at soil water content of 40% field capacity. The percentage of root infections from different sources of indigenous endomychorrhizae and different levels of soil water content is same i.e 100%.","PeriodicalId":13776,"journal":{"name":"International Journal of Biosciences","volume":"8 1","pages":"155"},"PeriodicalIF":0.0,"publicationDate":"2018-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89347872","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

THE USE OF BALIPLUS MINERAL FERTILIZER TO INCREASE PRODUCTION AND FARM INCOME OF Phaseolus vulgaris L. (Case Study in Andisol, Baturiti Tabanan Bali) 施用BALIPLUS矿肥提高菜豆产量和农业收入的研究(以巴厘地区andiol、Baturiti Tabanan Bali为例)

International Journal of Biosciences Pub Date : 2018-05-21 DOI: 10.24843/IJBB.2018.V05.I02.P08

I. Lanya, N. Subadiyasa

{"title":"THE USE OF BALIPLUS MINERAL FERTILIZER TO INCREASE PRODUCTION AND FARM INCOME OF Phaseolus vulgaris L. (Case Study in Andisol, Baturiti Tabanan Bali)","authors":"I. Lanya, N. Subadiyasa","doi":"10.24843/IJBB.2018.V05.I02.P08","DOIUrl":"https://doi.org/10.24843/IJBB.2018.V05.I02.P08","url":null,"abstract":"Increased production of foodstuffs is required in line with increasing population. The tourism sector in Bali needs quality products that are both viable and environmentally friendly. Previous research since 2002-2016, found mineral fertilizer plus can increase the highest production, , increased soil quality, and increased farm income. The research location was in Baturiti Subdistrict. The plant tested was string bean (Phaseolus vulgaris L) during first planting and residual effect of first fertilization (second planting). Eight fertilizer treatments: P0=control, P1=organic, P2= mineral, P3=chemical (NPK), P4=combination 1⁄2 (organic+chemical), P5=combination 1⁄2 (mineral+chemical), P6= Baliplus mineral fertilizer (BMF) =combination 1⁄2 (organic+mineral+chemical), and P7=combination 1⁄2(organic+mineral)], three replications. Parameters observed were bean weight, storage capacity, and value of farm income. The experimental designed used was Randomized Block Design, with Duncan's real test difference of 5%, using the Costat program. Fertilization was very influential and significantly affected to the production of the first planting and residual effects. The treatment using BMF in the first crop,resulted in the highest production (34.33 tons ha), highest production increase (62.92%), longest storage (36%) for one week, and the highest farm income (Rp 334 million ha). The P1 treatment showed the lowest production (25 ton ha), the lowest increase (18.45%), storage capacity (15.27%), and the lowest farm income (Rp 28.7 million ha). String bean with the highest residual effect (16.17 ton ha) was obtained in treatment P4. The average production of the remaining effect is 48.62% of the first crop. The use of mineral fertilizers plus bali can increase production, savings, and farm income, and reduce 50% of the use of chemical and organic fertilizers.","PeriodicalId":13776,"journal":{"name":"International Journal of Biosciences","volume":"3 1","pages":"147"},"PeriodicalIF":0.0,"publicationDate":"2018-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90097798","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

EVALUATION OF LAND SUITABILITY FOR INCREASING PRODUCTIVITY IN DEGRADED UNDA WATERSHED, DISTRICT OF KARANGASEM, BALI 巴厘岛karangasem地区退化的unda流域提高生产力的土地适宜性评价

International Journal of Biosciences Pub Date : 2017-12-01 DOI: 10.24843/ijbb.2017.v05.i01.p03

N. M. Trigunasih, I. N. Merit, W. Wiyanti, I. W. Narka, I. Dibia

{"title":"EVALUATION OF LAND SUITABILITY FOR INCREASING PRODUCTIVITY IN DEGRADED UNDA WATERSHED, DISTRICT OF KARANGASEM, BALI","authors":"N. M. Trigunasih, I. N. Merit, W. Wiyanti, I. W. Narka, I. Dibia","doi":"10.24843/ijbb.2017.v05.i01.p03","DOIUrl":"https://doi.org/10.24843/ijbb.2017.v05.i01.p03","url":null,"abstract":"Evaluation of land suitability to increase the productivity of degraded lands in the Unda watershed aims: providing a database of land suitability for land use planning in support of sustainable agricultural development, providing spatial information, and recommendations in accordance with the land use plan of inhibiting factors that exist. Identification of characteristic land quality evaluation survey carried out by the method of land with exploration area of research followed by soil sampling at some sample of land units. Land suitability classification using the criteria of the Technical Guidelines for Evaluation of Land For Agricultural Commodities of Soil Research Institute (2003) is by matching between the haracteristics of the land with the requirements of growing plants was evaluated. Types of agricultural crops that are evaluated include plantation crops and food crops hotikultura fruits. Suitability actual land (land suitability based on survey data) are mostly classified as unsuitable (N), and marginally suitable (S3). Qualities/characteristics as the land that is the main limiting factor, among others: quality of land erosion, slope, water availability, temperature, rooting media (soil texture and coarse material). Sustainable land use planning can be recommended with agroforestry, accompanied by vegetative conservation measures because it is cheaper and easier to be carried out by farmers. Recommended cultivation effort is a combination of timber plants with horticultural crops of fruits with the provisions of at least 40% must be enclosed timber. Plant timber that can be developed between the other plants that are not too heavy canopy.","PeriodicalId":13776,"journal":{"name":"International Journal of Biosciences","volume":"176 1","pages":"25"},"PeriodicalIF":0.0,"publicationDate":"2017-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73960299","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

GENETIC DIVERSITY AND FRUIT QUALITY OF SEVERAL POMELO “JERUK BALI” (Citrus grandis L. Osbeck) CULTIVARS IN BALI 巴厘几个柚子品种的遗传多样性和果实品质

International Journal of Biosciences Pub Date : 2017-12-01 DOI: 10.24843/ijbb.2017.v05.i01.p04

I. B. K. Mahardika, I. N. Rai, M. S. Mahendra, R. Dwiyani

{"title":"GENETIC DIVERSITY AND FRUIT QUALITY OF SEVERAL POMELO “JERUK BALI” (Citrus grandis L. Osbeck) CULTIVARS IN BALI","authors":"I. B. K. Mahardika, I. N. Rai, M. S. Mahendra, R. Dwiyani","doi":"10.24843/ijbb.2017.v05.i01.p04","DOIUrl":"https://doi.org/10.24843/ijbb.2017.v05.i01.p04","url":null,"abstract":"This study aimed to determine the genetic diversity and fruits quality of the \"Jeruk Bali\" cultivars grown in Bali. This research was conducted in all regencies and city in Bali, during 2016. Furthermore, several cultivars of “Jeruk Bali” were genetically analyzed based on RAPD markers using 10 primers. Analysis of the quality of fruit is based on physical properties and chemical content. Eighteen cultivars of \"Jeruk Bali\" obtained have a fruit morphological character with round, short round, and piriform fruit shapes, which are red, pink, cream and white flesh color. RAPD analysis results at 53% similarity level are grouped into 5 groups. The first group was only one cultivar, the second group consisted of 13 cultivars, the third and fourth groups were only one cultivar, while the fifth group consisted of two cultivars. The analysis of the diversity between cultivars based on the combination of physical and chemical properties of the fruit with hierarchy method on similarity level about 85% in a group is obtained by 4 (four) groups. Groupings by combination of physical and chemical properties of the fruit are not synchronized in their entirety with dendograms based on their genetic diversity. This illustrates the physicochemical properties of “Jeruk Bali” fruit in general is not fully genetical expressed, but also influenced by conditions of environmental growth.","PeriodicalId":13776,"journal":{"name":"International Journal of Biosciences","volume":"1 1","pages":"43"},"PeriodicalIF":0.0,"publicationDate":"2017-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88575498","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

THE EXISTENCE OF ENTOMOPATHOGENIC FUNGI ON RICE PLANTS RHIZOSPHERE 水稻根际昆虫病原真菌的存在

International Journal of Biosciences Pub Date : 2017-12-01 DOI: 10.24843/IJBB.2017.V05.I01.P02

N. Noerfitryani, H. Hamzah

引用次数: 5

EXPLORING STRIP INTERCROPPING POTENTIALS OF MAIZE-PULSE CROPS TO FIGHT CLIMATE VARIABILITY IMPACTS IN DRYLAND AREAS 探索玉米脉冲作物带状间作在干旱地区应对气候变率影响的潜力

International Journal of Biosciences Pub Date : 2017-09-01 DOI: 10.24843/IJBB.2017.V05.I01.P01

I. Jaya, S. Sudirman, Rosmilawati Rosmilawati

{"title":"EXPLORING STRIP INTERCROPPING POTENTIALS OF MAIZE-PULSE CROPS TO FIGHT CLIMATE VARIABILITY IMPACTS IN DRYLAND AREAS","authors":"I. Jaya, S. Sudirman, Rosmilawati Rosmilawati","doi":"10.24843/IJBB.2017.V05.I01.P01","DOIUrl":"https://doi.org/10.24843/IJBB.2017.V05.I01.P01","url":null,"abstract":"Recent climate variability affects maize production in dryland areas. This study aimed to explore potentials of strip intercropping of maize-pulse crops in improving productivity of dryland areas. The study was conducted in dryland area of Gumantar village, North Lombok (8.253654 S, 116.285695 E). Soil in that area was categorized as poor soil with the following properties: 0.46% organic matter, 0.05% N total (Kejdhal), available P 11.25 ppm (Olsen) and exchangeable K 0.77 me%, pH 7.0 and field capacity 29% (%/V). Rainfall data were collected during the growing seasons of 2016/2016 and 2016/2017. A field experiment of maize-pulse crops strip intercropping was conducted during a dry season of 2016. The component crops in the strip intercropping were maize NK212, maize NK7328, mungbean Vima-1 and groundnut Hypoma-1. All component crops were grown as monocropping and strip intercropping of maize-pulse crops in 8.4 x 5.0m plot size for each treatment. To measure productivity of the strip intercropping, relative yield total (RYT) and benefit to cost ratio (B/C) were calculated. They were great variations in rainfall in the last two years. From the experiment, data showed that all the strip intercropping treatments have RYT and B/C values >1 meaning that strip intercropping of maizepulse crops is more productive than monocropping and is feasible to be practiced in dryland areas. With the short growing period and their drought tolerant nature of the pulse crops, especially mungbean, the strip intercropping can be used to fight climate variability impacts in dryland areas.","PeriodicalId":13776,"journal":{"name":"International Journal of Biosciences","volume":"12 1","pages":"1"},"PeriodicalIF":0.0,"publicationDate":"2017-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80322832","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4