M. Daud, Manzoor Ahmad, I. Khan, U. Khan, S. Sabir, Aftab Ali, Mohsin Khan, Nadeemullah, Zohaib, Misal Khan, Ihsanullah, A. Hayat
{"title":"Pathogenic microbiological study of meat ready-to-eat and its products in different hotels of Peshawar, Khyber Pakhtunkhwa Pakistan.","authors":"M. Daud, Manzoor Ahmad, I. Khan, U. Khan, S. Sabir, Aftab Ali, Mohsin Khan, Nadeemullah, Zohaib, Misal Khan, Ihsanullah, A. Hayat","doi":"10.12692/ijb/6.4.106-111","DOIUrl":"https://doi.org/10.12692/ijb/6.4.106-111","url":null,"abstract":"","PeriodicalId":13776,"journal":{"name":"International Journal of Biosciences","volume":"45 1","pages":"106-111"},"PeriodicalIF":0.0,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79477858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
P. Karimzadeh, S. Zehtab-Salmasi, J. Shafagh-Kalvanagh, H. Janmohammadi
{"title":"Essential oil production of dill affected by different intercropping patterns with berseem clover and harvesting times.","authors":"P. Karimzadeh, S. Zehtab-Salmasi, J. Shafagh-Kalvanagh, H. Janmohammadi","doi":"10.12692/ijb/6.3.1-6","DOIUrl":"https://doi.org/10.12692/ijb/6.3.1-6","url":null,"abstract":"Intercropping is the practice of producing multiple crops in a given space. In this research berseem clover and dill were intercropped at different additive series (100:25, 100:50 and 100:75) at the Research Farm of the Faculty of Agriculture, University of Tabriz, Iran. Field experiment was arranged as split plot based on randomized complete block design with three replicates. Dill umbels were harvested at flowering, dough development and complete ripening stages. Results showed that among harvesting times, dough development stage and among intercropping patterns, 100:50 treatment had the highest essential oil percentage, essential oil yield and harvest index of essential oil. Berseem clover as a forage and legume crop promotes dill essential oil production and could be an effective plant in intercropping systems with essential oil bearing medicinal plants such as dill.","PeriodicalId":13776,"journal":{"name":"International Journal of Biosciences","volume":"6 1","pages":"1-6"},"PeriodicalIF":0.0,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85401646","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
T. Mehmood, S. Shafique, Qudsia Tabassam, M. Afzal, Saeed Ahmad
{"title":"Variation in antioxidant attributes, individual phenolic acids composition and biological activities of Thymus vulgaris: effects of extraction solvents.","authors":"T. Mehmood, S. Shafique, Qudsia Tabassam, M. Afzal, Saeed Ahmad","doi":"10.12692/ijb/6.11.73-86","DOIUrl":"https://doi.org/10.12692/ijb/6.11.73-86","url":null,"abstract":"","PeriodicalId":13776,"journal":{"name":"International Journal of Biosciences","volume":"200 1","pages":"73-86"},"PeriodicalIF":0.0,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85701968","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The impact of different soils and wastewater on castor bean (Ricinus communis L.) growth","authors":"Essam Kotb","doi":"10.12692/ijb/6.10.15-22","DOIUrl":"https://doi.org/10.12692/ijb/6.10.15-22","url":null,"abstract":"The oilseed plant castor bean (Ricinuscommunis L.) has many applications in medicine, industry and agriculture. This study aims to evaluate the growth of castor bean on different soils as affected by wastewater (sewage effluent) irrigation. Thus, castor bean seeds were sown in six different soils (sandy soil - sandy soil + compost - polluted soil - clay soil - calcareous soil - saline soil) and irrigated using equal amounts of sewage effluent. The physical and chemical properties of the soils used and the chemical constituents of sewage effluent were investigated. The results showed that the highest percentage of germination of castor bean seeds (96.6%) were achieved in the clay as well as sandy soil with added compost, followed by sandy soil (92.2%). On the other hand the lowest germination percentages were shown with the seeds sown in calcareous soil (50.3%) and saline soil (30.3%). The time duration required for the radical protrusion was also shortest (quick germination) and longest in the polluted, calcareous, and saline soils. There has been also a marked impact on the plant growth, fresh weight and dry weight gain of, where highest values were shown with the plants cultivated in clay soils followed by sandy soil with added compost. These plants exhibited in addition best criteria (weight, length, width). Thus, the results obtained confirmed the ability of castor beanto grow in different types of soils under irrigation by sewage effluent.","PeriodicalId":13776,"journal":{"name":"International Journal of Biosciences","volume":"11 1","pages":"15-22"},"PeriodicalIF":0.0,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73306525","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Isolating rbcL gene and promoter of bell pepper (Capsicum annuum L.) and its sequence analysis using bioinformatic tools","authors":"M. Samiee, B. Kohnehrouz","doi":"10.12692/ijb/6.2.395-402","DOIUrl":"https://doi.org/10.12692/ijb/6.2.395-402","url":null,"abstract":"Ru-BisCO (ribulose 1,5-bisphosphate carboxlyase) probobly is the most protein complex on the planet. Also it is the key enzyme in photosynthesis reactions in chloroplastic stroma to fix CO2. It is usually consisted of eight small and eight large subunits encoded by nucleus and plastids respectively. In this research, we have cloned rbcL gene and promoter from a common sweet bell pepper by polymerase chain reaction using total cellular DNA. Our results showed that length of coding sequences of rbcL gene in pepper is 1434 bp with 478 deduced amino acid residues. The insilico analysis of promoter region showed that -10 and -35 regions contain TACAAT and TTGCGC boxes respectively. Further analysis of cloned rbcL promoter form this kind of non spicy pepper elaborate that this promoter comprised motifs such as CAAT-box, HSE and circadian.","PeriodicalId":13776,"journal":{"name":"International Journal of Biosciences","volume":"48 1","pages":"395-402"},"PeriodicalIF":0.0,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77108910","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Kavand, A. Ebadi, Y. D. Shuraki, V. Abdossi, M. Mostafavy
{"title":"Evaluation of stability and uniformity in tissue culture-date palm (Phoenix dactylifera L.) plants of cv. Berhee by using morphological characteristics","authors":"A. Kavand, A. Ebadi, Y. D. Shuraki, V. Abdossi, M. Mostafavy","doi":"10.12692/ijb/6.1.117-122","DOIUrl":"https://doi.org/10.12692/ijb/6.1.117-122","url":null,"abstract":"Date palm (Phoenix dactylifera . L) is a dioecious, monocot and tropical fruit that requires pollination for fruit set and economically production. It is propagated by offshoot routinely. The low number of offshoot and short time of offshoot production are considered as the most disadvantages for its propagation. Since 20 th century, propagation of date palm through tissue culture techniques is conducted. Embryogenesis and organogenesis are the two main techniques for in vitro propagation of date palm. Somaclonal variation is the most problem in tissue culture of date palm that impose difficulties for growers. So, finding the easy and inexpensive method to control stability and uniformity are the most important factors in tissue culture propagation of date palm. In this experiment, we evaluated the qualities of tissue culture date palms c.v Berhee by analysis of some morphological characters. There was a lot of variation between propagated trees. It was noticed that some morphological factors can be considered as key factors to screen the type and off-type trees in this cultivar.","PeriodicalId":13776,"journal":{"name":"International Journal of Biosciences","volume":"2 1","pages":"117-122"},"PeriodicalIF":0.0,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82139999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Effect of pre-sowing treatments on seed germination in Quercus glauca Thunb., collected from different sampling sites of the Himalayan region of Pakistan.","authors":"Zulfiqar, Shujaul Mulk Khan, H. Ahmad","doi":"10.12692/ijb/6.11.42-48","DOIUrl":"https://doi.org/10.12692/ijb/6.11.42-48","url":null,"abstract":"This paper deals with the seed germination treatments of Quercus glauca Thunb., a member of the family Fagaceae a critically endangered species from the Himalayan region of Pakistan. Seeds of the species were collected from Mansehra, Batagram and Muzaffarabad Districts in November, 2013. Mature and ripened seeds were collected in suitable season from the selected sampling sites in polythene bags. Some of these collected seeds were scarified while others were subjected to stratification treatment only. Stratified treatments were given to the seeds for 10, 15 and 20 days in the laboratory. Others were scarified with knife and forceps. In stratified seeds, maximum germination rate i.e. 95.45% were observed in seeds collected from Batagram District while the lowest rate was observed in seeds collected from Mansehra District. Like-wise, for scarified treatment, maximum germination rate i.e. 100% was obtained in seeds collected from Batagram and Mansehra districts while in Muzaffarabad District lowest rate of germination i.e. 83% was observed. Scarified treatments proved to be more effective for this species as compared to stratified treatments. Therefore, these methods should be followed for the seed germination practices of these species and regeneration on larger scale.","PeriodicalId":13776,"journal":{"name":"International Journal of Biosciences","volume":"2 1","pages":"42-48"},"PeriodicalIF":0.0,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76309320","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Isolation and identification of Ulocladium sp. VL204 associated with Pistacia atlantica leaves from dana biosphere reserve, Jordan.","authors":"K. Alsharafa, M. Al-limoun","doi":"10.12692/ijb/6.6.119-126","DOIUrl":"https://doi.org/10.12692/ijb/6.6.119-126","url":null,"abstract":"Endophytic fungus isolated from Pistacia atlantica Desf. which associated with leaves necrotic spotting was identified using nuclear ribosomal DNA internal transcribed spacer regions (ITS1-5.8S rDNA-ITS2) as Ulocladium sp. VL204 a fungus member of Pleosporaceae family.","PeriodicalId":13776,"journal":{"name":"International Journal of Biosciences","volume":"79 1","pages":"119-126"},"PeriodicalIF":0.0,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87122382","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. H. Chattha, C. M. Hasfalina, Lee TeangShui, Benish Nawaz Mirani, M. R. Mahadi
{"title":"Effective and economic storage of wheat seed in straw-clay bin.","authors":"S. H. Chattha, C. M. Hasfalina, Lee TeangShui, Benish Nawaz Mirani, M. R. Mahadi","doi":"10.12692/ijb/6.3.83-93","DOIUrl":"https://doi.org/10.12692/ijb/6.3.83-93","url":null,"abstract":"The majority of farmers in Sindh province of Pakistan store wheat seed in traditional storage structures. These structures usually increase seed moisture and temperature of storage up to the levels that favor the proliferation of insects and fungi and results in seed deterioration. Considering the seed storage problem, the present study was performed. Straw-clay bins retain low moisture conditions for extended storage duration better than conventional bulk covered store. Seed stored in bulk covered store suffered severe deterioration caused by fungi, while straw-clay bins protected seed from these losses. Wheat seed stored for 12 months in straw-clay bins maintained better seed germination percentage, test weight, protein content and ash/mineral content than seed stored in bulk covered store. There was a minimum loss of quality of wheat in straw-clay bin as compare to bulk covered store. Hence, the adoption of the straw-clay bin must be encouraged in the developing countries to save their seeds and goods to reduce the losses as well as raise the food security, poverty alleviation and national economy.","PeriodicalId":13776,"journal":{"name":"International Journal of Biosciences","volume":"61 1","pages":"83-93"},"PeriodicalIF":0.0,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86060360","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Zamanipour, A. Tehranifar, E. G. Moghadam, B. Abedi
{"title":"Meristem culture of two sweet cherry cultivars cvs. \"Bing\" and \"Dovomras\"","authors":"M. Zamanipour, A. Tehranifar, E. G. Moghadam, B. Abedi","doi":"10.12692/ijb/6.5.178-185","DOIUrl":"https://doi.org/10.12692/ijb/6.5.178-185","url":null,"abstract":"The regeneration through meristem culture is an advanced biotechnological tehnique which is a very useful method for obtaining virus-free plants. In this study the effect of three media contains WPM, MS and QL and three different growth regulators combinations (0, 0.5 and 1 mgL -1 BA, 0.1 mgL -1 GA3 and 0.1 mgL -1 IBA) were investigated on regeneration of two Sweet cherry cultivars cvs. \"Bing\" and \"Dovomras\" in spring season. All experiments were arranged in completely randomized designed. Each treatment contained three replicates. Explants were soaked in 100 mgL -1 ascorbic acid and 150 mgL -1 citric acid for one hour before surface sterilization to prevent browning during in vitro culture. The meristem explants (0.5-0.7 mm) cultured in media and maintained at 26°C under a 16 hr-light/8 hr-dark with a light intensity of 2000-3000 lux from white fluorescent light. After six weeks, survival, necrosis and contamination percent were studied. The results showed that WPM medium induced the most survival percent in both cultivars. MS medium decreased the survival percent and maked the highest necrosis percent in both cultivars. Cultivars showed different responses to concentration of BA, so that, \"Bing\" cultivar in 1 mgL -1 BA and \"Dovomras\" in 0.5 mgL -1 BA had the best response (55.53-38.96%). The results also showed that \"Bing\" were more stable and had a higher survival","PeriodicalId":13776,"journal":{"name":"International Journal of Biosciences","volume":"33 1","pages":"179-185"},"PeriodicalIF":0.0,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82652026","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}