Isolating rbcL gene and promoter of bell pepper (Capsicum annuum L.) and its sequence analysis using bioinformatic tools

Abstract

Ru-BisCO (ribulose 1,5-bisphosphate carboxlyase) probobly is the most protein complex on the planet. Also it is the key enzyme in photosynthesis reactions in chloroplastic stroma to fix CO2. It is usually consisted of eight small and eight large subunits encoded by nucleus and plastids respectively. In this research, we have cloned rbcL gene and promoter from a common sweet bell pepper by polymerase chain reaction using total cellular DNA. Our results showed that length of coding sequences of rbcL gene in pepper is 1434 bp with 478 deduced amino acid residues. The insilico analysis of promoter region showed that -10 and -35 regions contain TACAAT and TTGCGC boxes respectively. Further analysis of cloned rbcL promoter form this kind of non spicy pepper elaborate that this promoter comprised motifs such as CAAT-box, HSE and circadian.