Meristem culture of two sweet cherry cultivars cvs. "Bing" and "Dovomras"

International Journal of Biosciences Pub Date : 2015-01-01 DOI:10.12692/ijb/6.5.178-185

M. Zamanipour, A. Tehranifar, E. G. Moghadam, B. Abedi

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Abstract

The regeneration through meristem culture is an advanced biotechnological tehnique which is a very useful method for obtaining virus-free plants. In this study the effect of three media contains WPM, MS and QL and three different growth regulators combinations (0, 0.5 and 1 mgL -1 BA, 0.1 mgL -1 GA3 and 0.1 mgL -1 IBA) were investigated on regeneration of two Sweet cherry cultivars cvs. "Bing" and "Dovomras" in spring season. All experiments were arranged in completely randomized designed. Each treatment contained three replicates. Explants were soaked in 100 mgL -1 ascorbic acid and 150 mgL -1 citric acid for one hour before surface sterilization to prevent browning during in vitro culture. The meristem explants (0.5-0.7 mm) cultured in media and maintained at 26°C under a 16 hr-light/8 hr-dark with a light intensity of 2000-3000 lux from white fluorescent light. After six weeks, survival, necrosis and contamination percent were studied. The results showed that WPM medium induced the most survival percent in both cultivars. MS medium decreased the survival percent and maked the highest necrosis percent in both cultivars. Cultivars showed different responses to concentration of BA, so that, "Bing" cultivar in 1 mgL -1 BA and "Dovomras" in 0.5 mgL -1 BA had the best response (55.53-38.96%). The results also showed that "Bing" were more stable and had a higher survival

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两个甜樱桃品种的分生组织培养。“Bing”和“domomras”

分生组织再生是一种先进的生物技术，是获得脱毒植株的重要途径。研究了WPM、MS和QL 3种培养基和3种不同生长调节剂组合(0、0.5和1 mg -1 BA、0.1 mg -1 GA3和0.1 mg -1 IBA)对2个甜樱桃品种的再生效果。春天的“Bing”和“domomras”。所有实验均采用完全随机设计。每个处理包含3个重复。外植体在100 mg -1抗坏血酸和150 mg -1柠檬酸中浸泡1小时，然后进行表面灭菌，防止离体培养过程中褐变。分生组织外植体(0.5-0.7 mm)在培养基中培养，在26℃、16小时光/8小时暗、2000-3000勒克斯白色荧光光强条件下维持。六周后，研究存活、坏死和污染百分比。结果表明，WPM培养基对两个品种的成活率均最高。MS培养基降低了两个品种的成活率，坏死率最高。不同品种对BA浓度的响应不同，其中“冰”和“多夫拉斯”在1 mg -1 BA和0.5 mg -1 BA浓度下的响应最佳(55.53 ~ 38.96%)。结果还表明，“Bing”更稳定，生存率更高

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International Journal of Biosciences

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