ANALYSIS ON RELATIONSHIP AMONG DENDROBIUM spp BALI BASED ON CHARACTERISTICS OF LEAVES ANATOMY

I. A. P. Darmawati, I. N. Rai, R. Dwiyani, I. Astarini
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引用次数: 2

Abstract

From explorations in several parts of the forest of Bali, 24 species of Dendrobium orchids were identified. Relationship among these species needed to be mapped for further development of orchids. Characteristics of leaves anatomy have been used to determine relations among plants. Characteristics of anatomy that were observed in this study from the paradermal and transversal cut of the leaves were shape and number of epidermis cells, location of stomata, the direction of stomata opening, shape and number of stomata, length of the epidermis and stomata, density of stomata, index of stomata, and thickness of mesophyll. Analysis of relations among species was displayed as a dendogram, using the software Minitab 17 Vis. Analysis of relationship among 24 species of Dendrobium Bali based on the leaves anatomy used grouped average method in squared Euclidean distance with the scale of 0.349 to 0.972. At Euclidean distance of 0.349, two clusters were formed where D. aloifolium and D. subulatum were separated from the other clusters. At 80 % similarity coefficient, seven clusters were formed using similar method, with the 3 and 5 clusters had the shortest distance (169.35). If species that were clustered together were cross-bred, the success rate was higher; meanwhile, the further away the relation between species, the smaller the success rate of cross breeding. The possibility of getting high-quality genotypes was higher when the cross-breeding was successful.
基于叶片解剖特征的巴厘岛石斛亲缘关系分析
通过对巴厘岛森林若干部分的考察,鉴定出了24种石斛兰。为了进一步开发兰花,需要绘制这些物种之间的关系图。叶子的解剖特征已被用来确定植物之间的关系。本研究从叶片的顺切和横切观察到的解剖特征是表皮细胞的形状和数量、气孔的位置、气孔开放的方向、气孔的形状和数量、表皮和气孔的长度、气孔密度、气孔指数和叶肉的厚度。采用Minitab 17 Vis软件,以树状图的形式进行种间关系分析。基于叶片解剖结构,采用欧几里得距离平方分组平均法,尺度为0.349 ~ 0.972。在欧几里得距离为0.349时,小叶金盏花和小叶金盏花形成两个簇;在相似系数为80%时,采用相似方法得到7个聚类,其中3和5聚类的距离最短(169.35)。如果聚集在一起的物种进行杂交,成功率会更高;同时,种间亲缘关系越远,杂交成功率越低。杂交成功,获得优质基因型的可能性较高。
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