International Journal of Agriculture and Biology最新文献_第5页

A Rapid Microtiter Assay to Evaluate Fungicide Sensitivity to Colletotrichum falcatum Isolates 快速微量滴定法评价杀菌剂对镰孢炭疽菌分离株的敏感性

International Journal of Agriculture and Biology Pub Date : 2022-02-01 DOI: 10.17957/ijab/15.1902

Renato de Carvalho Menezes

引用次数: 0

Validation of Functioning Resistant Genes against Malaysian Biotype of Brown Planthopper in Rice Variety, Rathu Heenati 水稻品种Rathu Heenati对马来西亚褐飞虱生物型功能抗性基因的验证

International Journal of Agriculture and Biology Pub Date : 2022-02-01 DOI: 10.17957/ijab/15.1896

Mohamad Bahagia Ab Ghaffar

{"title":"Validation of Functioning Resistant Genes against Malaysian Biotype of Brown Planthopper in Rice Variety, Rathu Heenati","authors":"Mohamad Bahagia Ab Ghaffar","doi":"10.17957/ijab/15.1896","DOIUrl":"https://doi.org/10.17957/ijab/15.1896","url":null,"abstract":"Rathu Heenati is a donor parent commonly used in the development of resistant rice varieties against brown planthopper (BPH), Nilaparvata lugens, which is a devastating insect pest of rice. The variety is reported to have a broad-spectrum resistance against BPH, through the action of multiple genes and quantitative trait loci (QTL) including Bph3, Qbph3, Qbph4 and Qbph10. The present study was carried out to validate the effectiveness of those genes against Malaysian BPH population. The study was conducted using an F2 segregating population which was obtained from a cross between Rathu Heenati and a Malaysian commercial variety, MR219. Plant damage score was used to estimate the degree of plant tolerance, while the amount of honeydew excretion was used to measure the level of antibiosis. The gene presence in an individual plant was determined based on the segregation pattern of the flanking microsatellite markers of the respective reported genes. Results confirmed the role of Bph3 and the three other QTLs in conferring resistance against the Malaysian biotype of BPH. Marker assisted breeding can facilitate the monitoring of the introgressed genes in the plants of a breeding population. © 2022 Friends Science Publishers","PeriodicalId":13769,"journal":{"name":"International Journal of Agriculture and Biology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44729287","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Assessment of the Impact of Rosemary Chitosan Microemulsion Effect on Escherichia coli and Listeria monocytogenes Dipping in Chicken Meat Stored at 4˚C 迷迭香壳聚糖微乳液对4℃冷藏鸡肉中大肠杆菌和单核增生李斯特菌的影响

International Journal of Agriculture and Biology Pub Date : 2022-02-01 DOI: 10.17957/ijab/15.1900

Dalia Y. Youssef

{"title":"Assessment of the Impact of Rosemary Chitosan Microemulsion Effect on Escherichia coli and Listeria monocytogenes Dipping in Chicken Meat Stored at 4˚C","authors":"Dalia Y. Youssef","doi":"10.17957/ijab/15.1900","DOIUrl":"https://doi.org/10.17957/ijab/15.1900","url":null,"abstract":"This article studies the potential use of the Rosemarry (REO) microemulsion with/without chitosan to assess the growth inhibition of Gram-positive and Gram-negative pathogenic bacteria represented by Listeria monocytogenes and Escherichia coli. The chicken samples were divided into four groups after inoculation of E. coli and L. monocytogenes separately: control group (without treatment), 1% chitosan nanoparticle treatment group, 0.5% REO microemulsion treatment group, and 0.5% REO + 1% chitosan microemulsion treatment group. Chicken samples were dipped into the treatments for 15 days of refrigerated storage at 4°C. All groups were kept refrigerated, and bacterial counts were taken on days 0, 1, 3, 6, 9, 12 and 15. Chitosan nanoparticle and REO microemulsion with/without chitosan were spherical shape and showed a narrow size distribution of 23.98 ± 0.83, 34.24 ± 2.2 and 28.01 ± 1.36 nm with a polydispersity index (PDI) of 0.86, 0.33 and 0.54, respectively indicating that greater homogeneity was achieved. REO chitosan microemulsion has 12 components detected by GC-Mass as follows: pinene (22.21%), borneol (21.32%), 1-(4-methoxyphenyl) ethanoneoxime (3.48%), oxocamphor (0.49%), camphor (2.99%), limonene (0.29%), cis-linalool oxide (0.87), 2-(5-chloro-methoxyphenyl) pyrrole (2.19%), homofarnesol (0.27%), levoverbenone (0.45%), peruviol (0.73%) and campesterol (1.22%). The results showed a substantial reduction in L. monocytogenes and E. coli count in all treatment groups when compared to the control group, with the greatest inhibitory efficacy in the 0.5% REO chitosan microemulsion group. A favorable effect of chitosan treatment on chicken acceptability during refrigerated storage was reported, with an improvement in the sensory qualities of the products. Thus, REO chitosan microemulsion is advised to be used in chicken to enhance resistance to harmful microorganisms. © 2022 Friends Science Publishers","PeriodicalId":13769,"journal":{"name":"International Journal of Agriculture and Biology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43921717","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nanoparticles Derived from Active Metabolites of Chaetomium cupreum CC3003 against Phytophthora Rot of Durian 铜毛霉CC3003抗榴莲疫霉活性代谢物制备的纳米颗粒

International Journal of Agriculture and Biology Pub Date : 2022-02-01 DOI: 10.17957/ijab/15.1894

R. Tongon

{"title":"Nanoparticles Derived from Active Metabolites of Chaetomium cupreum CC3003 against Phytophthora Rot of Durian","authors":"R. Tongon","doi":"10.17957/ijab/15.1894","DOIUrl":"https://doi.org/10.17957/ijab/15.1894","url":null,"abstract":"Phytophthora rot of durian (Durio zibetinus L.) is a serious disease wherever the crop has been planted and the disease control customarily uses chemical fungicides reported to be resistant by pathogen. Alternative non-chemical control strategies are being investigated to produce safe food. The main objective of this research was to test the activity of metabolites from Chaetomium cupreum CC3003 in the form of crude materials and nanoparticles to control and induce immunity to Phytophthora palmivora causing rot of durian var. Monthong. The results showed that P. palmivora proved to be pathogenic to durian var. Monthong. C. cupreum CC3003 acted as an antagonist and P. palmivora was confirmed as the pathogen by morphological and molecular genetic identification. Effective doses (ED50) of CC-E, CC-H and CC-M crude metabolites for spore inhibition were 60, 97 and 140 mg.kg-1, respectively. The research findings found that the diameters of nano CC-E, nano CC-H and nano CC-M were 534, 499 and 537 nm, respectively. The nano CC-E, nano CC-H and nano CC-M demonstrated antifungal activity against P. palmivora with ED50 of 11, 13 and 16 mg.kg-1, respectively. The nanoparticles at low concentrations were more effective than crude metabolites at high concentrations. Nano-CC-E used to treat seedlings of durian resulted in the production of scopoletin which served as an immunity agent or elicitor against rot disease of durian. It is concluded that active metabolites derived from C. cupreum significantly inhibited P. palmivora and induced immunity through phytoalexin production. © 2022 Friends Science Publishers","PeriodicalId":13769,"journal":{"name":"International Journal of Agriculture and Biology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41919584","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Effect of Phosgreen Fertilization on the Growth and Phosphorus Uptake of Lettuce (Lactuca sativa) 光绿施肥对生菜生长和磷吸收的影响

International Journal of Agriculture and Biology Pub Date : 2022-02-01 DOI: 10.17957/ijab/15.1892

A. Jama-Rodzeńska

{"title":"The Effect of Phosgreen Fertilization on the Growth and Phosphorus Uptake of Lettuce (Lactuca sativa)","authors":"A. Jama-Rodzeńska","doi":"10.17957/ijab/15.1892","DOIUrl":"https://doi.org/10.17957/ijab/15.1892","url":null,"abstract":"The effect of Phosgreen fertilizer on the growth, development and biometric traits of lettuce was determined in comparison to superphosphate on two selected horticultural media. The aim of the experiment was to evaluate the effect of Phosgreen on the basis of the reaction of tested plants. Lettuce was grown on two horticultural media: mineral soil and deacidified peat. This study determined phosphorus content and uptake as a phosphorus fertilizer effect on different horticultural media. Lettuce developed and grew better on deacidified peat. Based on this study, it can be concluded that struvite is a phosphorus fertilizer with great potential and warrants further testing. A significant increase in lettuce head mass, number of leaves and rosette width under the influence of struvite was found compared to control conditions. The increase in rosette mass compared to the control was approximately 54%. The width of the rosette increased by circa 32% due to the use of Phosgreen compared to the control and by 3% compared to superphosphate. Both P content and uptake by lettuce under Phosgreen was comparable to that under superphosphate. Cu, Zn, Pb and Cd content in lettuce leaves under Phosgreen fertilization was comparable to that under superphosphate. © 2022 Friends Science Publishers","PeriodicalId":13769,"journal":{"name":"International Journal of Agriculture and Biology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46934242","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Effect of Different Doses of Acute Gamma Radiation on the Cultivation of Volvariella volvacea 不同剂量急性伽玛辐射对草粪栽培的影响

International Journal of Agriculture and Biology Pub Date : 2022-02-01 DOI: 10.17957/ijab/15.1898

Fatin Nabilah Fisol

{"title":"Effect of Different Doses of Acute Gamma Radiation on the Cultivation of Volvariella volvacea","authors":"Fatin Nabilah Fisol","doi":"10.17957/ijab/15.1898","DOIUrl":"https://doi.org/10.17957/ijab/15.1898","url":null,"abstract":"In mushroom cultivation, acute gamma radiation can be used and studied to improve the production of its fruiting body. Therefore, the objective of this study was to investigate the effect of gamma radiation on the mycelial growth and production of the fruiting body of Volvariella volvacea (Bull.) Singer. The mycelia were exposed to gamma radiation at various doses: 0 (control), 300, 600, 900, 1200 and 1500 Gy. The irradiated mycelial growth rate was recorded on potato dextrose agar (PDA) and spawning substrate. The spawn was then produced by inoculating the treated mycelia on paddy straw and subsequently cultivated on an empty fruit bunch to compare the production. The result showed no significant difference in mycelial growth rate on wheat, the number of mushrooms, and mushroom weight between control and treatments of 300, 600 and 900 Gy. However, there was the presence of hairy structures on the fruiting bodies of V. volvacea in all spawns with irradiated mycelia and the production of clustered fruiting bodies from spawns treated with 1200 Gy observed. The effect of treatments on the texture of fruiting bodies was insignificant. Although acute gamma radiation did not significantly increase the production of V. volvacea compared to the control treatment, there were morphological changes observed on the V. volvacea fruiting body which can be studied further. © 2022 Friends Science Publishers","PeriodicalId":13769,"journal":{"name":"International Journal of Agriculture and Biology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47401208","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Modified Atmosphere as an Alternative Measure for Controlling of Sitophilus oryzae Reared on Different Stored Grains 改良大气作为控制不同储粮稻瘟病菌的替代措施

International Journal of Agriculture and Biology Pub Date : 2022-02-01 DOI: 10.17957/ijab/15.1901

Muhammad Mohsin

{"title":"Modified Atmosphere as an Alternative Measure for Controlling of Sitophilus oryzae Reared on Different Stored Grains","authors":"Muhammad Mohsin","doi":"10.17957/ijab/15.1901","DOIUrl":"https://doi.org/10.17957/ijab/15.1901","url":null,"abstract":"Protection of grains and their products from insect pests remained a big constraint in the way of food security. Current study evaluated the effective exposure time to kill the Sitophilus oryzae (L.), reared on two different diets viz., wheat and maize under six Carbon dioxide (CO2) concentrations. Modified atmospheres (MA) contained 25, 30, 35, 40, 45 and 50% CO2 by volume at ambient temperature, respectively. Twenty S. oryzae adults, along with a 20 g diet, were released in each airtight exposure chamber (150 mL capacity). A measured quantity of CO2 gas (99.9%) was released in exposure chamber by the injection syringe from gas cylinder. Mortality data were recorded after 24, 48, 72, 96 and 120 h. The mortality rates varied between the insect cultures reared on maize and wheat diets. S. oryzae, reared on a wheat diet, showed higher mortality after exposure to all CO2 concentrations as compared to the insect culture reared on the maize diet. At 45% CO2concentration, maximum mortality (100%) was observed after 120 h in case of maize reared insects and (100%) after 96 h in case of wheat reared S. oryzae. The results revealed that Carbon dioxide, as an eco-friendly approach, may be used as the best alternative method to minimize the pest infestation in stored products to avoid insecticide resistance development in stored grain insect pests. The Carbon dioxide is not included in the category of toxic gases and has no detrimental or residual effect in the stored grains. © 2022 Friends Science Publishers","PeriodicalId":13769,"journal":{"name":"International Journal of Agriculture and Biology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49535340","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Seagrass Debris as Source of Fiber and Bioactive Compounds in Feed for Dairy Goats 海草碎屑作为奶山羊饲料中纤维和生物活性化合物的来源

International Journal of Agriculture and Biology Pub Date : 2021-12-01 DOI: 10.17957/ijab/15.1888

Y. Hachana

{"title":"Seagrass Debris as Source of Fiber and Bioactive Compounds in Feed for Dairy Goats","authors":"Y. Hachana","doi":"10.17957/ijab/15.1888","DOIUrl":"https://doi.org/10.17957/ijab/15.1888","url":null,"abstract":"Climate change has severely affected rainfall regularity, limiting grass growth and thus fodder availability. Finding unconventional resources has become a challenge for small and medium breeders to feed their livestock. The objective of this study is to determine whether the use of marine plant debris, Posidonia oceanica, as total or partial replacement of oat hay in alpine goat diets will affect or not milk yield and quality. The sea grass was characterized according to the sampling site. Chemical and phytochemical analysis revealed significantly higher levels of dry matter, minerals, total polyphenols and carotenoids in P. oceanica on banquettes than in floating Posidonia. The condensed tannin content was almost the same for both sites. Partial or total replacement of oat hay by P. oceanica in alpine goat rations significantly increased milk production and milk fat content, while somatic cell count decreased significantly. In addition, P. oceanica significantly enhanced milk biochemical composition by increasing flavonoid and total phenol contents. However, protein, lactose and freezing point parameters were not affected. Sensory analysis revealed substantial improvement in the organoleptic quality of P. oceanica fed goats’ milk compared to the other group, which was highly appreciated by most of the panelists. The seagrass P. ocanica could therefore be a safe solution to improve fodder availability for small and medium breeders, reduce production costs and increase milk market value. © 2021 Friends Science Publishers","PeriodicalId":13769,"journal":{"name":"International Journal of Agriculture and Biology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42918667","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of Lactic Acid Bacteria from Luwak (Paradoxurus hermaphroditus) Gastrointestinal Tract Luwak（Paradourus hermachroditus）胃肠道乳酸菌的鉴定

International Journal of Agriculture and Biology Pub Date : 2021-12-01 DOI: 10.17957/ijab/15.1887

fitriyana fitri

{"title":"Identification of Lactic Acid Bacteria from Luwak (Paradoxurus hermaphroditus) Gastrointestinal Tract","authors":"fitriyana fitri","doi":"10.17957/ijab/15.1887","DOIUrl":"https://doi.org/10.17957/ijab/15.1887","url":null,"abstract":"Luwak is a mammal that is widely cultivated to produce luwak coffee. Luwak coffee is a rare and one of the world's most expensive coffees from Indonesia. Numerous enzymes and microorganisms, many of which are lactic acid bacteria, contribute to the production of the coffee aroma. This research aims to identify lactic acid bacteria from luwak digestion accurately down to the species level. The parameters tested in this study were Gram staining, bacterial resistance to acid, bacterial resistance to bile salts, and genotypic identification of isolates using the 16S rRNA gene sequencing method. The results found that US1, FS3, FS4 and FS6 were identified as Leuconostoc pseudomesenteroides, US2 and US3 were identified as Lactobacillus plantarum, FS2 was identified as Weissella cibaria and FS5 was identified as Staphylococcus haemolyticus. All of these isolates were included in Gram-positive based on the results of Gram staining. However, among the 8 isolates obtained, one isolate, namely FS5 (S. haemolyticus), was not a lactic acid bacterium, but a pathogenic bacterium. This information can be used for the development of luwak origin bacteria for in vitro coffee fermentation purposes. © 2021 Friends Science Publishers","PeriodicalId":13769,"journal":{"name":"International Journal of Agriculture and Biology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42427145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Depletion and Distribution Studies for Oxytetracycline in Broiler Chicken using Commercial ELISA with Subsequent Confirmatory Analysis by HPLC-UV 应用商用酶联免疫吸附试验研究土霉素在肉鸡体内的消耗和分布，并辅以HPLC-UV验证分析

International Journal of Agriculture and Biology Pub Date : 2021-12-01 DOI: 10.17957/ijab/15.1890

Mehwish Mumtaz

{"title":"Depletion and Distribution Studies for Oxytetracycline in Broiler Chicken using Commercial ELISA with Subsequent Confirmatory Analysis by HPLC-UV","authors":"Mehwish Mumtaz","doi":"10.17957/ijab/15.1890","DOIUrl":"https://doi.org/10.17957/ijab/15.1890","url":null,"abstract":"Tetracyclines are commonly used in livestock for the treatment of various diseases. Their residues above the maximum residual limit-MRL (200 μg kg-1) can cause health hazard issues not only in humans but also in animals. For this purpose, efforts were made to study distribution and depletion profile of Oxytetracycline in broiler chicken by ELISA and HPLC. Regarding this, an experiment was conducted on healthy chickens of average weight 600 ± 20 g. Six broiler chickens were treated with formulation (OXTRA L.A.) containing Oxytetracycline dehydrate @20 ppk solution (150 μL) equivalents to recommended dose 20 mg kg-1 through intramuscular route, while control birds were untreated. Treated chickens were slaughtered at an interval of 1, 8, 16, 32, 64 and 120 h. Different tissue samples including liver, kidney, thigh and chest muscles were collected and screened by ELISA (Cat No. 5091TC, Euro Proxima). Lowest detection limit (IC20) was calculated as 0.12 ng mL-1 and middle of the test (IC50) 0.5 ng mL-1 with recovery 75 to 86%. Reverse phase liquid chromatography (HPLC-UV) method was also standardized by using range of standards from 25 to 200 μg kg-1. Calibration curve showed good response with correlation coefficient (R2) 0.9727 and recovery 85 to 93%. Overall, withdrawal period (< MRL) in all tissue samples was calculated as 117 h (4.8 days) by ELISA while 128 h (5 days) by HPLC-UV. Both validated methods can be further utilized to generate reliable data for food safety measures in Pakistan to enhance international trade. © 2021 Friends Science Publishers","PeriodicalId":13769,"journal":{"name":"International Journal of Agriculture and Biology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45354647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0