International Journal of Agriculture and Biology最新文献

Antioxidant Activity and Phytochemical Profile in Sequential Solvent Extract of Faloak (Sterculia quadrifida) Leaves and Stem Bark 枇杷叶、茎皮序贯溶剂提取物的抗氧化活性及植物化学特征

International Journal of Agriculture and Biology Pub Date : 2025-03-08 DOI: 10.17957/ijab/15.2323

Finna Setiawan

{"title":"Antioxidant Activity and Phytochemical Profile in Sequential Solvent Extract of Faloak (Sterculia quadrifida) Leaves and Stem Bark","authors":"Finna Setiawan","doi":"10.17957/ijab/15.2323","DOIUrl":"https://doi.org/10.17957/ijab/15.2323","url":null,"abstract":"Faloak (Sterculia quadrifida R.Br) is an endemic plant of East Nusa Tenggara Island. This plant was found in the East Nusa Tenggara archipelago, including Timor, Sumba, Flores, Alor and Rote Islands. Traditionally, the community used this plant for a variety of therapeutic purposes. Faloak demonstrate numerous biological activities, primarily attributable to its secondary metabolite compounds. The presence of phenolic compounds and flavonoids renders it a good candidate for the development of a novel natural antioxidant resource. Faloak possesses several biological activities related to its high antioxidant content. However, research on antioxidant, phenolic, and flavonoids content of Faloak leaves and stembark in different solvent remains limited. This study aims to investigate the antioxidant activity of Faloak leaves and stembark the ABTS (2, 2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) and DPPH (2, 2-diphenyl-1- (2, 4, 6-trinitrophenyl) hydrazyl) radical scavenging assay in different solvents. Measurement of phenolic and flavonoids levels, together with thin layer chromatography (TLC) bioautography, is performed to predict the related antioxidant compounds. The results showed that ethanol, as a polar solvent, produced the highest output, indicating that Faloak mostly comprises a polar compound. Leaves have a higher concentration of phenolic and flavonoid compounds than stembark. Leaves and stembark have significant antioxidant activity. Thin layer chromatography (TLC) combined with bioautography confirmed the presence of polyphenols and flavonoids, which are likely responsible for the antioxidant activity in the Faloak fractions. Bioautography also yielded active compounds with antioxidant activity. The stembark and leaves of Faloak possess antioxidant properties; nevertheless, the leaves demonstrate significant potential as a candidate for diverse pharmacological treatments due to their robust antioxidant activity and sustainability.","PeriodicalId":13769,"journal":{"name":"International Journal of Agriculture and Biology","volume":"33 06","pages":"330603-330603"},"PeriodicalIF":0.0,"publicationDate":"2025-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://doi.org/10.17957/ijab/15.2323","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147904256","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microbe-Derived Antioxidants Promote the Expression of Nutrient Transporters through Regulating Apoptosis and Autophagy in Mice Challenged with Diquat 微生物来源的抗氧化剂通过调节Diquat刺激小鼠的细胞凋亡和自噬促进营养转运蛋白的表达

International Journal of Agriculture and Biology Pub Date : 2025-01-01 DOI: 10.17957/ijab/15.1180

Xindan Chen, Takami Sho, Weina Xu, Jing Zhang, Jianxiong Xu

{"title":"Microbe-Derived Antioxidants Promote the Expression of Nutrient Transporters through Regulating Apoptosis and Autophagy in Mice Challenged with Diquat","authors":"Xindan Chen, Takami Sho, Weina Xu, Jing Zhang, Jianxiong Xu","doi":"10.17957/ijab/15.1180","DOIUrl":"https://doi.org/10.17957/ijab/15.1180","url":null,"abstract":"We evaluated the effects and underlying mechanisms of microbe-derived antioxidants (MA) on the expression of nutrient transporters in mice challenged with diquat.Thirty C57BL/6 female mice were randomly divided into 3 groups: Control and Diquat group, both orally administered with 0.9% saline each day; MA+Diquat group, administered with 0.065 mg/mL day -1 MA.At day 22, the mice were injected with 0.9% saline (Control) or 1.8 mg/mL diquat (Diquat and MA+Diquat groups) and sacrificed for jejunum samples after 3 h.Results showed that oral administration of MA alleviated diquat-induced intestinal oxidative stress, as evidenced by the increased (P < 0.05) activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px), enhanced (P < 0.05) content of total glutathione (T-GSH) and total antioxidant capacity (T-AOC), and decreased (P < 0.05) contents of hydrogen peroxide (H 2 O 2 ) and malondialdehyde (MDA).Compared with the Diquat group, pretreatment with MA elevated (P < 0.05) the mRNA expression of glucose transporters, fatty acid transporters and oligopeptide and amino acid transporters.Notably, diquat supplementation increased (P < 0.05) the concentrations of caspase-3, caspase-8 and caspase-9, whereas such increases were inhibited (P < 0.05) by MA supplementation in diquat challenged mice.In addition, complementation of MA elevated (P < 0.05) the mRNA and protein expression of microtubleassociated protein light chain 3 (LC3) and beclin-1 in diquat challenged mice.Collectively, our findings indicate that the promoted expression of nutrient transporters in MA-pretreated mice appears to be associated with the enhanced autophagy and the inhibited apoptosis.","PeriodicalId":13769,"journal":{"name":"International Journal of Agriculture and Biology","volume":"22 5","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://doi.org/10.17957/ijab/15.1180","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147922749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Steroidal Saponin Ypsilandra thibetica Induces Apoptosis in Colorectal Carcinoma Cells through the Mitochondrion-Dependent Reactive Oxygen Species Pathway 甾体皂苷通过线粒体依赖的活性氧途径诱导结直肠癌细胞凋亡

International Journal of Agriculture and Biology Pub Date : 2025-01-01 DOI: 10.17957/ijab/15.1067

Zhendong Zhao, Li Xia, Dandan Xu, Guohong Zhou, Huiqing Huang, Daiying Zhou, Jin Lin, Shaolin Li, Jia Liu

{"title":"Steroidal Saponin Ypsilandra thibetica Induces Apoptosis in Colorectal Carcinoma Cells through the Mitochondrion-Dependent Reactive Oxygen Species Pathway","authors":"Zhendong Zhao, Li Xia, Dandan Xu, Guohong Zhou, Huiqing Huang, Daiying Zhou, Jin Lin, Shaolin Li, Jia Liu","doi":"10.17957/ijab/15.1067","DOIUrl":"https://doi.org/10.17957/ijab/15.1067","url":null,"abstract":"The whole plant of Ypsilandra thibetica Franch, a perennial herb, has the officinal functions in heat-clearing, detoxifying and relieving congeation.YB16, one of the steroidal saponin in Y. thibetic, exerts anticancer activity in prostate cancer and lung cancer.However, the bioactivity and underlying mechanisms of YB16 in colorectal carcinoma (CRC) are not understood.In the present study, by MTT, apoptosis, mitochondrial membrane potential levels and intercellular reactive oxygen species (ROS) assays, YB16 inhibited the proliferation of cells and induced apoptosis in CRC HCT116 and HCT8 cells.Meanwhile, YB16 provoked mitochondrial membrane potential depolarizing in a concentration-dependent manner.Further analysis showed that YB16 induced apoptosis via stimulating the production of intercellular ROS and the antioxidant NAC reversed the apoptosis in HCT116 and HCT8 after YB16 treatment.These datas demonstrated that YB16 induced the apoptosis in CRC cell lines via mitochondrial-dependent ROS pathway and YB16 might be a potential anticancer drug to CRC.","PeriodicalId":13769,"journal":{"name":"International Journal of Agriculture and Biology","volume":"22 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://doi.org/10.17957/ijab/15.1067","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147896708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Function of Nitric Oxide in Chitosan Oligosaccharide-Induced Resistance to Tobacco Mosaic Virus 一氧化氮在壳寡糖诱导的烟草花叶病毒抗性中的作用

International Journal of Agriculture and Biology Pub Date : 2025-01-01 DOI: 10.17957/ijab/15.0866

Hongyan Zhang, Heng Yin, Guojie Jin

{"title":"Function of Nitric Oxide in Chitosan Oligosaccharide-Induced Resistance to Tobacco Mosaic Virus","authors":"Hongyan Zhang, Heng Yin, Guojie Jin","doi":"10.17957/ijab/15.0866","DOIUrl":"https://doi.org/10.17957/ijab/15.0866","url":null,"abstract":"Chitosan oligosaccharide (COS) has been shown to protect plants against viral invasion.To study its antiviral mechanism, nitric oxide (NO) signaling was investigated in the process of resistance to tobacco mosaic virus (TMV) in tobacco plants induced by COS.Results show that COS and NO donor sodium nitroprusside could induce NO generation quantified by Griess reagent in tobacco leaves with no TMV infection.However, the NO levels were not markedly different in various samples after TMV infection.The content of S-nitrosothiols, an important signal molecule downstream of NO was also determined, and results indicate that they may not be involved in the COS-induced antiviral process.In addition, western blotting experiments show that COS and sodium nitroprusside were observed to inhibit TMV multiplication and movement, which were blocked by NO scavenger cPTIO.COS and sodium nitroprusside-induced callose deposition were also reversed by treatment with cPTIO in aniline blue assay.The expression of -1, 3-glucanase, which has been proved to control callose degradation, was regulated by COS and NO.Another protein, calreticulin related with TMV movement was also modulated by COS and NO.Collectively, these observations suggest that NO participates in COS-induced resistance to TMV by regulating calreticulin and -1, 3-glucanase expression.","PeriodicalId":13769,"journal":{"name":"International Journal of Agriculture and Biology","volume":"21 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://doi.org/10.17957/ijab/15.0866","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147888907","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification and Quantification of Chemical Constituents in Daphne altaica and their Antioxidant and Cytotoxic Activities 杜鹃花化学成分的鉴定与定量及其抗氧化和细胞毒活性

International Journal of Agriculture and Biology Pub Date : 2025-01-01 DOI: 10.17957/ijab/15.1159

Tiantian Zhang, Fu-Yao Wei, Shuwei Liu, Shujie Zhao, Lei Liu, Huiying Zhao, Yuxin Li

引用次数: 0

Agrobacterium rhizogenes-Mediated CRISPR/Cas9 Base Editing of CaSal1 Gene Enhances Drought Tolerance in Chickpea 根际农杆菌介导的CRISPR/Cas9碱基编辑CaSal1基因增强鹰嘴豆抗旱性

International Journal of Agriculture and Biology Pub Date : 2024-12-08 DOI: 10.17957/ijab/15.2270

Naglaa Y. Abdallah

{"title":"Agrobacterium rhizogenes-Mediated CRISPR/Cas9 Base Editing of CaSal1 Gene Enhances Drought Tolerance in Chickpea","authors":"Naglaa Y. Abdallah","doi":"10.17957/ijab/15.2270","DOIUrl":"https://doi.org/10.17957/ijab/15.2270","url":null,"abstract":"Drought stress poses a significant challenge to agriculture in the context of climate change, as it restricts chickpea yield and leads to financial losses. Chickpea (Cicer arietinum L.) is the second most widely farmed legume worldwide. This work aimed to examine the impact of altering the Sal1 gene, which encodes the 3’-phosphoadenosine 5’-phosphate phosphatase, on drought tolerance in chickpea. The CRISPR-based gene editing technique was employed for this purpose. Considering the constraints imposed by gene delivery systems and the laborious and expensive tissue culture procedure, we opted for the utilization of Agrobacterium rhizogenes. The designed sgRNA was inserted into the expression vector, pDW3873, which contains the nuclease null variant, nCas9 D10A, coupled to the PmCDA1 cytosine deaminase at the C-terminal, then injected into the A. rhizogenes strain K599. In this study, CRISPR/Cas9 was used to make precise changes to a specific genomic area in CaSal1 gene. Results demonstrated that a total of 6 chickpea plants were identified as transgenic plants. Their drought tolerance was enhanced and root showed double branches of the control but shorter in length. This article demonstrates that the CDB system effectively facilitates gene editing in a sterile-free environment, eliminating the requirement for tissue culture. Consequently, it is well-suited for the transformation of a substantial quantity of plants. This technique can also be utilized on several species that have the ability to produce root suckers.","PeriodicalId":13769,"journal":{"name":"International Journal of Agriculture and Biology","volume":"33 02","pages":"330210-330210"},"PeriodicalIF":0.0,"publicationDate":"2024-12-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://doi.org/10.17957/ijab/15.2270","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147922930","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Dynamic Response of Ryegrass (Lolium multiflorum) to Drought Stress – A Case Study of Natural Drought Modeling 黑麦草（Lolium multiflorum）对干旱胁迫的动态响应——以自然干旱模拟为例

International Journal of Agriculture and Biology Pub Date : 2024-08-21 DOI: 10.17957/ijab/15.2216

Qin Liu, Tajie Sangzhu, Yang Luo, Ping Deng, Yunqi Zhao, Ge Zhang, Jing Li, Hao‐Zheng Li, Manjun Yang

{"title":"Dynamic Response of Ryegrass (Lolium multiflorum) to Drought Stress – A Case Study of Natural Drought Modeling","authors":"Qin Liu, Tajie Sangzhu, Yang Luo, Ping Deng, Yunqi Zhao, Ge Zhang, Jing Li, Hao‐Zheng Li, Manjun Yang","doi":"10.17957/ijab/15.2216","DOIUrl":"https://doi.org/10.17957/ijab/15.2216","url":null,"abstract":"Ryegrass (Lolium multiflorum Lam. cv. Blue Heaven) is an annual forage cultivar for more than one-half of the carrying capacity. Because ryegrass is dependent on water availability, drought during emergence severely affects grain yield in a global warming world. In this research, the dynamic profile of drought-inducible metabolic response was determined. Compared with well-watered conditions (1st day), from the 2nd to 14th day after stress, the ryegrass samples were collected every two days and measured for the content of total saccharides, sucrose, glucose, ascorbic acid, free amino acid, total polyphenol, chlorophyll, reduced and oxidative glutathione, reactive oxidative species, malondialdehyde, superoxide anion, cellulose, lignin and hemicellulose. Under the daily evaporation of 27.18 ± 3.4 g/day, the dynamic response to drought tolerance of ryegrass fluctuated on the whole rather than linear change. The first eight days had light and moderate drought stress, and ryegrass actively reduced the drought stress damage through the metabolic and antioxidant system. After eight days, ryegrass suffered from severe stress, and its metabolic and antioxidant system were collapsed and plants died. The dynamic response to light and moderate stress (the first 8 days) were not consistent with that of severe stress (last 6 days) and even had opposite results. Therefore, it is necessary to determine the optimized treatment by dynamic monitoring in drought stress. More importantly, optimal daily evaporation, moderate and severe stress should be selected according to the needs of drought tolerance research. Overall, this study provided a novel and comprehensive insight of the dynamic response to drought stress.","PeriodicalId":13769,"journal":{"name":"International Journal of Agriculture and Biology","volume":"32 04","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://doi.org/10.17957/ijab/15.2216","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147880929","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Possible Protective Effect of Luteolin in a Thioacetamide Rat Model of Testicular Toxicity 木犀草素在硫代乙酰胺大鼠睾丸毒性模型中的可能保护作用

International Journal of Agriculture and Biology Pub Date : 2022-12-01 DOI: 10.17957/ijab/15.1993

Sahar J. Melebary

{"title":"The Possible Protective Effect of Luteolin in a Thioacetamide Rat Model of Testicular Toxicity","authors":"Sahar J. Melebary","doi":"10.17957/ijab/15.1993","DOIUrl":"https://doi.org/10.17957/ijab/15.1993","url":null,"abstract":"Luteolin is a flavone that serves as a natural antioxidant. The therapeutic impacts of luteolin is influenced by its antioxidant, anti-inflammatory, anticancer, neuroprotective and antineoplastic properties. This study aimed to establish an animal model of testicular toxicity caused by Thioacetamide (TAA). In addition, high doses of Luteolin (LUT) were supplemented to observe the role of LUT in attenuating spermato-toxicity, the hazard of oxidative stress, and testicular histopathological alterations induced by TAA. Thirty adult rats were equally divided into three groups as follow; G1: negative control group, G2: was given TAA 200 mg/kg body weight, G3: was received LUT at a dose of (50 mg/kg body weight) for four weeks concurrently with TAA. During this experiment histological, immunohistochemical, biochemical and morphometric measures were evaluated. TAA revealed loss of normal architecture of testicular tissue, wide interstitial spaces, and a loss of stratal arrangement of germinal epithelium with intercellular spacing. Also, a reduction in the number of +ve vimentin staining Sertoli cells with a marked reduction in the mean of vimentin-positive cells increased oxidative stress in testicular tissue. LUT protected testis against these alterations. By reducing oxidative stress, histological and immunohistochemical alterations and restoring the normal testicular tissue architecture and function, LUT successfully lowers TAA testicular toxicity in albino rats, recommending that it may have similar effects in humans. © 2022 Friends Science Publishers","PeriodicalId":13769,"journal":{"name":"International Journal of Agriculture and Biology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45897841","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

‘MARKAZ-2019’: A Spring Wheat Variety for Rainfed Areas of Pakistan “MARKAZ-2019”:巴基斯坦雨养地区的春小麦品种

International Journal of Agriculture and Biology Pub Date : 2022-05-01 DOI: 10.17957/ijab/15.1931

M. Qamar

{"title":"‘MARKAZ-2019’: A Spring Wheat Variety for Rainfed Areas of Pakistan","authors":"M. Qamar","doi":"10.17957/ijab/15.1931","DOIUrl":"https://doi.org/10.17957/ijab/15.1931","url":null,"abstract":"‘Markaz-2019’ is a spring wheat (Triticum aestivum L.) cultivar developed and released by the wheat breeding program at the Crop Sciences Institute, National Agricultural Research Center, Islamabad, Pakistan, in 2019. The cultivar was approved by the Punjab Seed Council of Pakistan in its 51st meeting (19th September 2019) held at the Punjab Seed Corporation, Lahore, Pakistan. Markaz-2019 is of known pedigree “SOKOLL//FRTL/2*PIFED”, derived and selected from the 19th Semi-Arid Wheat Yield Trial (SAWYT) entry number (332) from CIMMYT during 2011–2012. Following evaluation of this candidate, ‘NR-449’ was used as candidate line for five growing seasons at NARC-Islamabad and in multi-location trials and then was officially named as Markaz-2019. Trials were conducted under rainfed conditions and grain yield and quality parameters such as grain color, size and 1000-grain weight were tested. This cultivar is a medium height wheat (107 cm) with semi-erect growth habit, stiff and hollow stem, medium to high grain volume weight in kilogram/ hectoliter (78.7 kg/hL), medium maturity (160 days), medium to high grain protein (151 g kg-1) and flour protein concentration (151 and 105 g kg-1). Markaz-2019 was released for its high yield, (ranging from 4500 kg ha-1 to 6050 kg ha-1), its improved resistance to stripe rust (Puccinia striiformis var. tritici) and leaf rust (P. triticina) and tolerance to drought. Its grain yield was significantly higher than check cultivars, and therefore recommended for cultivation in rainfed areas of Pakistan. © 2022 Friends Science Publishers","PeriodicalId":13769,"journal":{"name":"International Journal of Agriculture and Biology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48476633","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Improvement of Micropropagation through Combination of Plant Growth Regulators in Indonesian Sorghum Hybrid Cultivar 植物生长调节剂组合改良印尼高粱杂交种的微繁性

International Journal of Agriculture and Biology Pub Date : 2022-05-01 DOI: 10.17957/ijab/15.1930

Wulan Nursyiam Ningtiyas

{"title":"Improvement of Micropropagation through Combination of Plant Growth Regulators in Indonesian Sorghum Hybrid Cultivar","authors":"Wulan Nursyiam Ningtiyas","doi":"10.17957/ijab/15.1930","DOIUrl":"https://doi.org/10.17957/ijab/15.1930","url":null,"abstract":"Sorghum is a tropical grass used for various purposes including food, animal feed, and biofuels source, but its in vitro propagation still found an obstacle due to tissue browning. This study aims to propagate and regenerate callus in the Indonesian sorghum hybrid, named Numbu cultivar from seed and meristematic leaf whorl explants. The combined effect of plant growth regulators (PGRs), namely 2,4-D and proline as well as NAA (naphthalene acetic acid) and BAP (6-benzyl amino purine) was investigated on embryogenic callus induction and regeneration, respectively. The results showed that callus induction was significantly faster in the seed explants compared to leaf whorl. The optimal medium used to obtain a large induction in the seed explants was MS salt containing 3 mg L-1 2,4-D and 5000 mg L-1 proline, which gave a 68% yield. Furthermore, the best medium used to obtain a high plant regeneration frequency was the MS medium containing 0.1 mg L-1 NAA and 3 mg L-1 BAP, which regenerated 78% of the callus into plantlet. The addition of 560 mg L-1 proline to the combination medium increased doubled the regeneration into the planlet. Based on the results, PGRs combinations are required to improve embryogenic callus induction and regeneration into planlet in sorghum. © 2022 Friends Science Publishers","PeriodicalId":13769,"journal":{"name":"International Journal of Agriculture and Biology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46846518","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0