Improvement of Micropropagation through Combination of Plant Growth Regulators in Indonesian Sorghum Hybrid Cultivar

Q2 Agricultural and Biological Sciences
Wulan Nursyiam Ningtiyas
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引用次数: 0

Abstract

Sorghum is a tropical grass used for various purposes including food, animal feed, and biofuels source, but its in vitro propagation still found an obstacle due to tissue browning. This study aims to propagate and regenerate callus in the Indonesian sorghum hybrid, named Numbu cultivar from seed and meristematic leaf whorl explants. The combined effect of plant growth regulators (PGRs), namely 2,4-D and proline as well as NAA (naphthalene acetic acid) and BAP (6-benzyl amino purine) was investigated on embryogenic callus induction and regeneration, respectively. The results showed that callus induction was significantly faster in the seed explants compared to leaf whorl. The optimal medium used to obtain a large induction in the seed explants was MS salt containing 3 mg L-1 2,4-D and 5000 mg L-1 proline, which gave a 68% yield. Furthermore, the best medium used to obtain a high plant regeneration frequency was the MS medium containing 0.1 mg L-1 NAA and 3 mg L-1 BAP, which regenerated 78% of the callus into plantlet. The addition of 560 mg L-1 proline to the combination medium increased doubled the regeneration into the planlet. Based on the results, PGRs combinations are required to improve embryogenic callus induction and regeneration into planlet in sorghum. © 2022 Friends Science Publishers
植物生长调节剂组合改良印尼高粱杂交种的微繁性
高粱是一种热带草本植物,用于各种用途,包括食品、动物饲料和生物燃料来源,但由于组织褐变,其体外繁殖仍然存在障碍。本研究旨在利用种子和分生组织叶轮芽外植体在印尼高粱杂交种Numbu中繁殖和再生愈伤组织。研究了植物生长调节剂2,4-D和脯氨酸以及NAA(萘乙酸)和BAP(6-苄基氨基嘌呤)对胚性愈伤组织诱导和再生的联合作用。结果表明,与叶轮芽相比,种子外植体的愈伤组织诱导明显更快。在种子外植体中获得大诱导的最佳培养基是含有3mg L-1 2,4-D和5000mg L-1脯氨酸的MS盐,其产率为68%。此外,获得高植株再生频率的最佳培养基是含有0.1mg L-1 NAA和3mg L-1 BAP的MS培养基,其将78%的愈伤组织再生成植株。向组合培养基中添加560mg L-1脯氨酸使植株再生加倍。基于这些结果,PGRs组合需要改善高粱胚性愈伤组织的诱导和再生。©2022 Friends Science出版社
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来源期刊
International Journal of Agriculture and Biology
International Journal of Agriculture and Biology AGRICULTURE, MULTIDISCIPLINARY-
CiteScore
1.70
自引率
0.00%
发文量
40
审稿时长
5 months
期刊介绍: Information not localized
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