International Journal of Agriculture and Biology最新文献_第6页

Gas Chromatography-Mass Spectroscopy and Histopathological Effects of Methanol Leaf Extract of Uvaria chamae on the Midgut of Sitophilus zeamais 气相色谱-质谱联用技术及其对玉米象中肠组织病理学的影响

International Journal of Agriculture and Biology Pub Date : 2021-12-01 DOI: 10.17957/ijab/15.1884

D. Oboho

{"title":"Gas Chromatography-Mass Spectroscopy and Histopathological Effects of Methanol Leaf Extract of Uvaria chamae on the Midgut of Sitophilus zeamais","authors":"D. Oboho","doi":"10.17957/ijab/15.1884","DOIUrl":"https://doi.org/10.17957/ijab/15.1884","url":null,"abstract":"The present study was carried out to assess the effect of methanol leaf extract of Uvaria chamae using Gas Chromatography-Mass Spectrum (GC-MS) to determine the phytochemicals present and its effect on the histology of midgut of maize weevil, Sitophilus zeamais. Insects were administered with 10 mg/kg of the plant extract using diffusion method where insects were put in a petri dish containing various concentrations and observed to see the stage they begin to die due to toxicity and observed for 5 min. They were collected into foil processing paper and fixed in Bouins fluid for 24 h, repacked after 24 h and folded in fresh foil immersed in buffered formalin for histopathological studies. Result revealed that a severe degeneration de-arrangement of the respiratory tract epithelial lining, secretory lining cells and gastrointestinal layers with the destruction of the muscular layer when compared with the control. The methanol leaf extracts of U. chamae were preliminary screened for the phytochemicals. The extract shows the presence of cardiac glycosides, saponin, steroids/terpenes, flavonoids, alkaloids and phenols. GC-MS analysis of the extract showed the presence of 2-nitrobenzaldehyde (4.00), malic acid (2.04), L-aspartic acid (2.00), 1, 1, dimethylhydrazine (1.86), Cedrandiol (1.75), 2-amino-4-(2-methylpropenyl)-pyrimidin-5-carboxylic acid (1.56), thiirane (1.54), mercaptoethanol (1.11) and some minor compounds. The findings indicated that methanol extract of U. chamae is rich in phyto-compounds having biological activities on the midguts’ histology of S. zeamais. Therefore, it is recommended as an alternative for the synthetic insecticide used by farmers for the preservation of stored grains. © 2021 Friends Science Publishers","PeriodicalId":13769,"journal":{"name":"International Journal of Agriculture and Biology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47489060","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characteristics of Canary Hair Sheep´s Breed Carcass Fed Banana (Musa acuminate) By-Products: Effects on Regional Tissue Composition, pH and Color 香蕉（Musa acuminate）副产品饲养的金丝雀毛羊胴体特性：对区域组织组成、pH值和颜色的影响

International Journal of Agriculture and Biology Pub Date : 2021-09-01 DOI: 10.17957/ijab/15.1853

S. Ahmed

{"title":"Characteristics of Canary Hair Sheep´s Breed Carcass Fed Banana (Musa acuminate) By-Products: Effects on Regional Tissue Composition, pH and Color","authors":"S. Ahmed","doi":"10.17957/ijab/15.1853","DOIUrl":"https://doi.org/10.17957/ijab/15.1853","url":null,"abstract":"The contributions of replacing rye-grass (Lolium spp.) hay with banana (Musa acuminata) by-products on carcass and tissue characteristics, pH and color, in the Canary hair sheep breed were studied. Twenty-two lambs with an initial live weight of 14.8 ± 2.5 kg were individually housed and fed two different diets for 58 days: The first group (experimental diet) received as forage a mixture of fresh banana (Musa acuminata) by-products composed by leaves and pseudo-stem. The other one got commercial rye-grass (Lolium spp.) hay (conventional diet) as a fiber source. The two groups got an additional commercial concentrate food (CON). After the 58 days growing trial (24.3 kg ± 1.0 kg), seven lambs of each group (n=14) were slaughtered in the experimental slaughterhouse. The muscle, the pH, and the color were measured at the time immediately after slaughter and 24 h later, using the muscles Longissimus thoracis et lumborum and semimembranosus. At that time (after 24 h), the remaining semi-carcass was butchered and dissected for analysis of the carcass´s regional and tissue composition. Although there is no significant difference on the tissue composition, carcass regional and the muscle pH, there are significant differences in the color in the Longisimus toracis et lumborum between the two assessed diets. The incorporation of banana by-products in a diet to fatten lambs Canary hair sheep breed would not grossly alter the carcass quality, at least based on the assessed parameters. Banana by-products as a feeding resource can maintain animal productivity and meat quality. © 2021 Friends Science Publishers","PeriodicalId":13769,"journal":{"name":"International Journal of Agriculture and Biology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43812541","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Broccoli Seedling Production in Response to Recognised Organic Inputs 响应公认的有机输入的西兰花幼苗生产

International Journal of Agriculture and Biology Pub Date : 2021-09-01 DOI: 10.17957/ijab/15.1854

J. Rodríguez-Ortiz

{"title":"Broccoli Seedling Production in Response to Recognised Organic Inputs","authors":"J. Rodríguez-Ortiz","doi":"10.17957/ijab/15.1854","DOIUrl":"https://doi.org/10.17957/ijab/15.1854","url":null,"abstract":"This study evaluated the production of seedlings broccoli (Brassica oleracea var. Italica) with organic inputs. The inputs were as follows; a) growth medium, consisting of Sphagnum peat (Pro Moss TBK®) mixed with poultry manure compost (Vertia® brand) in a) 90:10 and 80:20 ratios; b) biofungicide Trichoderma harzianum Rifai (Natucontrol® brand) at doses of 1.5 and 3 g/L water per 338-cavity polystyrene tray; and c) complementary nutrition applied in irrigation with poultry manure tea at doses of 0.5 and 1 dS/m per tray every two days. Control set was a ‘typical management’ control based on peat (100%) as a growing medium with the application of conventional fertiliser (1 g/L of Tricel® 20 every two days) and conventional fungicide Mancozeb as a damping-off preventative (1 g/L per tray). The seedling growth, relative chlorophyll content, photosystem II quantum yield, and morphological indicators showed that the eight treatments with recognised organic inputs performed significantly better than the control (p<0.05). The use of peat substrate mixed with poultry manure (80:20 ratio) with inoculation of T. harzianum at a dose of 1.5–3 g/L and with application of poultry manure tea at a dose of 1 dS/m yielded the best results. We determined that it is possible to obtain quality broccoli seedlings with the inputs recognised for certified organic agriculture. © 2021 Friends Science Publishers","PeriodicalId":13769,"journal":{"name":"International Journal of Agriculture and Biology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49540092","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Sources and Doses of Nitrogen Associated with Inoculation with Azospirillum brasilense Modulate Growth and Gas Exchange of Corn in the Brazilian Amazon 接种巴西氮螺旋菌相关氮的来源和剂量对巴西亚马逊地区玉米生长和气体交换的调节

International Journal of Agriculture and Biology Pub Date : 2021-08-01 DOI: 10.17957/ijab/15.1843

J. G. Palheta

{"title":"Sources and Doses of Nitrogen Associated with Inoculation with Azospirillum brasilense Modulate Growth and Gas Exchange of Corn in the Brazilian Amazon","authors":"J. G. Palheta","doi":"10.17957/ijab/15.1843","DOIUrl":"https://doi.org/10.17957/ijab/15.1843","url":null,"abstract":"The specific objective of the study was to evaluate effect of inoculation with Azospirillum brasilense and nitrogen (N) doses on vegetative growth and gas exchange in Zea mays L. The experimental design adopted was the completely randomized, in a 4 2 2 factorial scheme, in the following way: four doses of N (0 60 120 and 180 kg ha-1 of N), two sources of N (common urea and urease inhibitor-treated urea) and absence and presence of inoculation with A. brasilense, with four replications. The evaluations were made for vegetative growth of the plant (plant height, stem diameter, leaf area, number of leaves, dry mass of stem, root, leaves and aerial part and total dry mass) and photosynthesis, stomatal conductance, transpiration, internal carbon, relationship between internal and external carbon and content of chloroplast pigments. The application of N provided an improvement in plant growth, and, in general, the dose of 180 kg ha-1 N associated with A. brasilense, promoted an increase in stem diameter, photosynthesis, stomatal conductance, transpiration and internal carbon ratio of the corn. The treatment with urease inhibitor, greatly promoted the stem diameter, transpiration, Ci/Ca ratio and chlorophyll (Chl) a, b, total compared to urea treatment. The inoculation of the corn seeds with the bacteria and the use of N fertilization, regardless of the source, promoted an improvement in the vegetative growth of the hybrid, improving the vegetative growth and the physiological responses of corn when applied to the highest dose of 180 kg/ha N. © 2021 Friends Science Publishers","PeriodicalId":13769,"journal":{"name":"International Journal of Agriculture and Biology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47710580","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Authentication, Micromorphology and Ultrastructure of Pollen Grains and Seeds of Endemic Taxa in Saint Katherine Protectorate, South Sinai, Egypt 埃及南西奈圣凯瑟琳保护区特有红豆杉花粉粒和种子的鉴定、显微形态和超微结构

International Journal of Agriculture and Biology Pub Date : 2021-08-01 DOI: 10.17957/ijab/15.1844

M. EL-KHALAFY

{"title":"Authentication, Micromorphology and Ultrastructure of Pollen Grains and Seeds of Endemic Taxa in Saint Katherine Protectorate, South Sinai, Egypt","authors":"M. EL-KHALAFY","doi":"10.17957/ijab/15.1844","DOIUrl":"https://doi.org/10.17957/ijab/15.1844","url":null,"abstract":"The endemic taxa were restricted to a specific geographic region and they are essential for setting conservation priorities. This study aimed to update the endemic taxa list in Saint Katherine Protectorate (SKP) depending on literature reviews, field trips and herbaria consultation. Other characters also recorded viz, sex forms, dispersal types and flowering time. Also, the morphological characters of the pollen grains and seeds were examined and photographed using light microscope (LM) and scanning electron microscope (SEM). In addition, the mineral composition of pollens and seeds was detected using energy dispersive X-ray (EDX). The updated list included 13 taxa belonging to 11 genera and 8 families. All the recorded taxa were bisexual; ballochores were the most represented dispersal type. There was a gradual increase in the endemic taxa from March to August while decreasing from October to February. Pollens were isopolar and medium in size. They possessed colpate, colporate, or porate aperatus, as well as reticulate exine sculpture. Furthermore, operculum and margo were absent in most of the pollens. The seed colour ranged from light brown to black; elliptic; basal hilum; polygonal and irregular-shaped seeds were the most represented. All previous characters were diagnosed at generic and specific levels, which helped in the construction of artificial keys to facilitate the differentiation between the studied taxa. The present study has the priority in describing pollens and seeds of Astragalus fresenii and Micromeria serbaliana, in addition to the description of the seeds of Ballota kaiseri. The presence and percentage of twelve elements detected by EDX differed significantly within the investigated pollen grains and seeds. The present data indicated that pollen grains and seeds of studied taxa had high percentages of carbon, oxygen, phosphorous, magnesium, nitrogen and calcium. This study is the first attempt using EDX technique with these taxa. © 2021 Friends Science Publishers","PeriodicalId":13769,"journal":{"name":"International Journal of Agriculture and Biology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48211649","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Arbuscular Mycorrhizal Fungi Enhance Sorghum Plant Growth under Nitrogen-Deficient Conditions through Activation of Nitrogen and Carbon Metabolism Enzymes 丛枝菌根真菌通过激活氮碳代谢酶促进缺氮条件下高粱的生长

International Journal of Agriculture and Biology Pub Date : 2021-08-01 DOI: 10.17957/ijab/15.1825

A. Kchikich

{"title":"Arbuscular Mycorrhizal Fungi Enhance Sorghum Plant Growth under Nitrogen-Deficient Conditions through Activation of Nitrogen and Carbon Metabolism Enzymes","authors":"A. Kchikich","doi":"10.17957/ijab/15.1825","DOIUrl":"https://doi.org/10.17957/ijab/15.1825","url":null,"abstract":"Nitrogen (N), one of the most important elements for plant growth, is needed by plants in large quantities. However, this nutrient has limited supply in the soil. Arbuscular mycorrhizal fungi (AMF) are known for their ability to form symbiotic association with plants and transfer the mineral nutrients to the host plants. To validate this hypothesis on sorghum plants, three ecotypes of this cereal (3p4, 3p9 and 4p11) were cultivated with and without AMF under low nitrogen concentration (0.5 mM NH4+). Growth parameters were determined and key enzymes responsible for nitrogen and carbon metabolisms such as glutamine synthetase (GS), glutamate dehydrogenase (GDH), phosphoenolpyruvate carboxylase (PEPC), isocitrate dehydrogenase (ICDH), malate dehydrogenase (MDH) and asparate aminotransferase (AAT) were measured. For the three sorghum ecotypes, mycorrhizal plants showed a higher plant growth compared to the control plants. The biochemical parameters revealed a significant increase in the nitrogen assimilatory enzymes; GS and GDH in the leaves and roots of mycorrhizal plants. Furthermore, mycorrhizal fungi also appear to have a significant effect on carbon assimilatory enzymes. These enzymes are known to have a cardinal role in the provision of carbon skeletons essential for the assimilation of ammonium and thus, amino acids synthesis. Our study indicates clearly that AMF can be an efficient way to optimize nitrogen uptake and/or assimilation by plants and thus improve the crop yields with lower amount of nitrogen fertilizers. © 2021 Friends Science Publishers","PeriodicalId":13769,"journal":{"name":"International Journal of Agriculture and Biology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47451421","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Productivity of Phosphorus Fertilization in Cowpea-Maize Strip Intercropping under Rainfed Conditions 旱作条件下大豆-玉米带状间作磷肥产量的研究

International Journal of Agriculture and Biology Pub Date : 2021-08-01 DOI: 10.17957/ijab/15.1834

J. Asiwe

{"title":"Productivity of Phosphorus Fertilization in Cowpea-Maize Strip Intercropping under Rainfed Conditions","authors":"J. Asiwe","doi":"10.17957/ijab/15.1834","DOIUrl":"https://doi.org/10.17957/ijab/15.1834","url":null,"abstract":"Crop yields are declined due to low soil fertility, insufficient soil water availability and poorly managed cropping systems in Limpopo province of South Africa. Phosphorus (P) is a major essential nutrient element required by crops for enhanced growth and development. Interactions between different rates of P fertilization and strip intercropping system have not been studied in detail under rainfed conditions in semi-arid region of Limpopo province. Therefore, this study was conducted to assess the performance of four cowpea varieties at four levels (0, 15, 30, 45 kg P ha-1) of P fertilization in a cowpea-maize intercropping system in a split-split plot design during two seasons. Significant interactions were obtained between variety and phosphorus application as well as variety and cropping system for 90% physiological maturity, root mass and grain yield in both seasons. P levels significantly influenced and enhanced grain yield, land equivalent ratio, profit and benefit cost ratio achieved. PAN311 and TVu13464 matured earlier across P levels and they were selected promising cowpea varieties based on their early maturity and high yield. Land equivalent ratio values were greater than 1.0, which indicated performance and advantage of an intercropping system over monocropping system in land utilisation. The optimum P level for cowpea-maize strip intercropping was at 30 kg P ha-1 based on yield and financial return. The results showed that P application enhanced the productivity of the cowpea varieties in cowpea-maize strip intercropping in the semi-arid environment of Limpopo province. © 2021 Friends Science Publishers","PeriodicalId":13769,"journal":{"name":"International Journal of Agriculture and Biology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42193647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Expression Changes of Genes Related to Germination Based on EST Database under Priming Treatment by Gibberellic Acid in Perilla frutescens (Korean Perilla) 基于EST数据库的赤霉素诱导下紫苏萌发相关基因的表达变化

International Journal of Agriculture and Biology Pub Date : 2021-08-01 DOI: 10.17957/ijab/15.1831

E. Seong

{"title":"Expression Changes of Genes Related to Germination Based on EST Database under Priming Treatment by Gibberellic Acid in Perilla frutescens (Korean Perilla)","authors":"E. Seong","doi":"10.17957/ijab/15.1831","DOIUrl":"https://doi.org/10.17957/ijab/15.1831","url":null,"abstract":"It is very important to establish an optimal seed priming process in order to increase the vitality of the seeds and promote the metabolism for the germination of the seeds. The optimum concentrations and species of priming agents to improve seed germination of both medicinal plants were also estimated. To improve the germination rate of Perilla frutescens(Korean perilla) seeds, various seed priming agents were used to analyze seed germination rates in the Saeyeopsil, Okdong and 141 collection Korean perilla cultivars. The agents used for seed priming were CaCl2, Ca(NO3)2, NaCl, K3PO4, polyethylene glycol, and gibberellic acid (GA3). When 0.1 mMGA3was used for seed priming, germination rates of Okdong, and the 141 collection showed a greater than 70% increase compared to the controls. Nine genes were selected for expression analysis by searching for genes related to seed germination and plant development in the EST(Expressed Sequence Tag) database of the Korean perilla cDNA library. GA3priming treatment for 1 d induced higher transcriptional levels of genes related to germination and plant developmentthan controls treated with water only. These genes were identified as protochlorophyllide reductase-like, magnesium-chelatase subunit ChlI, heme-binding protein 2-like, glyceraldehyde 3-phosphate dehydrogenase A, Chlorophyll a-b binding protein 6, B2 protein, 2-Cys peroxiredoxin BAS1, and 21 kDa protein. From these results, we suggest that when priming Korean perilla seeds with GA3, a large number of genes involved in plant development at early stages of seed germination play a role in improving the seed germination rate. Also, these induced genes are ideal candidate biomarkers for seed priming of Korean perilla. Specially, protochlorophyllide reductase-like is thought to be a potential gene for future molecular marker.© 2021 Friends Science Publishers","PeriodicalId":13769,"journal":{"name":"International Journal of Agriculture and Biology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41806589","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhancement of Cellulose Rich Organic Matter Degradation by Inoculation with Streptomyces sp. Strains 链霉菌菌株接种促进富含纤维素的有机物降解

International Journal of Agriculture and Biology Pub Date : 2021-08-01 DOI: 10.17957/ijab/15.1832

Simonida Djuri

{"title":"Enhancement of Cellulose Rich Organic Matter Degradation by Inoculation with Streptomyces sp. Strains","authors":"Simonida Djuri","doi":"10.17957/ijab/15.1832","DOIUrl":"https://doi.org/10.17957/ijab/15.1832","url":null,"abstract":"Microbial degradation of organic matter is a vital part of carbon cycle in nature. Actinobacteria play an important role in the decomposition of cellulose rich organic matter (CROM). Streptomyces spp. are abundant in soil, produce various secondary metabolites and secrete extracellular enzymes. The aim of this research was to isolate and select Streptomyces strains with the best cellulose degradation abilities. Out of total 32 actinobacteria isolates, four Streptomyces strains (CA1, CA10, PA2 and PA7) were subjected to morphological, physiological, biochemical characterization and molecular identification. CROM degradation potential of the strains was investigated on straw and beech briquettes as well as on legume based substrate in in vitro condition. Streptomyces strains CA1 and CA10 showed the best cellulose production and starch hydrolysis abilities, followed by strains PA2 and PA7. Strain CA1 was also positive to production of pectinase enzymes. Streptomyces zaomyceticus CA1 and S. tanashiensis CA10 were used as inoculants, which degraded the raw cellulose from 38.38 to 81.69% in the investigated substrates (straw, beech, legume), during a 30-day incubation experiment. CROM inoculation with the selected Streptomyces strains improved and accelerated its degradation in controlled conditions. © 2021 Friends Science Publishers","PeriodicalId":13769,"journal":{"name":"International Journal of Agriculture and Biology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42989103","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impact of Seed Treatments with Fungal Biocontrol Agents on Enzymatic Activities and Phenolic Content of Soybean under Greenhouse and Field Conditions 温室和大田条件下真菌生物防治剂对大豆酶活性和酚类含量的影响

International Journal of Agriculture and Biology Pub Date : 2021-08-01 DOI: 10.17957/ijab/15.1837

A. Intisar

{"title":"Impact of Seed Treatments with Fungal Biocontrol Agents on Enzymatic Activities and Phenolic Content of Soybean under Greenhouse and Field Conditions","authors":"A. Intisar","doi":"10.17957/ijab/15.1837","DOIUrl":"https://doi.org/10.17957/ijab/15.1837","url":null,"abstract":"Fungi in the genus Trichoderma are widely used as biological control agents because they can suppress plant pathogens and activate plant defense systems. In the present study, efficacy of microbial antagonists viz., T. harzianum and T. viride or their combination was evaluated against the pathogenic fungus Macrophomina phaseolina and their effect on enzymatic activities and phenol content of soybean [Glycine max (L.) Merr.] plants. Soybean seeds were inoculated with T. harzianum and T. viride separately or in combination, and sown in pots under green house and under field conditions. Host enzymatic activities and phenol levels were measured at 14, 28 and 42 days after sowing (DAS) in both field and greenhouse experiments. Seed treatments with T. harzianum, T. viride or their combination increased peroxidase, polyphenol oxidase and β-1, 3-glucanase activities, and also the total phenol content in soybean leaves as compared to a non-treated control treatment. Concentration of peroxidase and β-1, 3-glucanasepeaked at 14 DAS and decreased thereafter in all the treatments under greenhouse and field conditions. All the treatments showed the highest levels of total phenols and polyphenol-oxidase at 28 DAS under both greenhouse and field conditions. At 14 DAS in both trials, the combination of T. viride + T. harzianum resulted in the highest level of peroxidase and β-1, 3-glucanase activities. This combination also resulted in the highest levels of total phenols and polyphenol oxidase content at 28 DAS. Our findings demonstrated that application of Trichoderma species as seed treatment has potential to trigger key mechanisms of systemically acquired resistance in soybean, and thereby enhanced efficacy of disease management tactics. © 2021 Friends Science Publishers","PeriodicalId":13769,"journal":{"name":"International Journal of Agriculture and Biology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48466704","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0