International Journal of Agriculture and Biology最新文献

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Comparative Transcriptomic Analyses of Chlorogenic Acid Biosynthesis Pathways in Diploid and Triploid Camellia sinensis 二倍体和三倍体茶树绿原酸生物合成途径的转录组学比较分析
International Journal of Agriculture and Biology Pub Date : 2021-08-01 DOI: 10.17957/ijab/15.1840
Chao Shen
{"title":"Comparative Transcriptomic Analyses of Chlorogenic Acid Biosynthesis Pathways in Diploid and Triploid Camellia sinensis","authors":"Chao Shen","doi":"10.17957/ijab/15.1840","DOIUrl":"https://doi.org/10.17957/ijab/15.1840","url":null,"abstract":"Chlorogenic acid (CGA), as a kind of depside in plants, has a variety of beneficial effects on human health, which also plays an important role in helping plants resist a variety of stresses. Therefore, the biosynthetic pathway of CGA has been studied in many plants, however, the synthesis of CGA has not been well elucidated in Camellia sinensis. In our research, different CGA levels were detected between triploid tea variety „Qianfu 4‟ and diplont tea variety „Qianmei 419‟ using HPLC and the CGA content in triploid Camellia sinensis was greater than that in diploid Camellia sinensis. Transcriptome sequencing for diploid and triploid Camellia sinensis was employed to explore genes associated with CGA biosynthesis. Finally, 154,097 unigenes were obtained in total, of which 891 may be related to the biosynthesis of CGA. Furthermore, differentially expressed genes (DEGs) were screened between diploid and triploid Camellia sinensis, 32 DEGs were discovered to be related to CGA biosynthesis, including sixteen phenylalanine ammonia lyase (PAL) genes, three 4-coumarate coenzyme A ligase (4CL) genes, nine cinnamate 4-Hydroxylase (C4H) genes, four Hydroxycinnamoyl-CoA shikimate/quinate hydroxycinnamoyl transferase (HQT/HCT), and two hundred and twenty-one TFs including eighty-eight ERFs, forty-one bZIPs, forty-two MYBs and fifty WRKYs, which may also play an important role in the biosynthesis of CGA. Our results will lay the foundation for further exploration of the biosynthesis of CGA and revealing the related regulatory network in Camellia sinensis. © 2021 Friends Science Publishers","PeriodicalId":13769,"journal":{"name":"International Journal of Agriculture and Biology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44591437","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Growth and Development of Soybean Plants with the Pat Gene under different Glufosinate Rates 不同草甘膦用量下含Pat基因大豆植株的生长发育
International Journal of Agriculture and Biology Pub Date : 2021-08-01 DOI: 10.17957/ijab/15.1827
T. T. Mundt
{"title":"Growth and Development of Soybean Plants with the Pat Gene under different Glufosinate Rates","authors":"T. T. Mundt","doi":"10.17957/ijab/15.1827","DOIUrl":"https://doi.org/10.17957/ijab/15.1827","url":null,"abstract":"The pat gene confers tolerance to glufosinate in transgenic soybean plants; however, some aspects of the selectivity of this herbicide need to be better elucidated. This study aimed to evaluate the development of soybean plants with the pat gene under the application of different glufosinate rates. The experiment was conducted in a greenhouse and included seven different glufosinate rates of and two soybean cultivars (LL0291 and LL0767) with the pat gene, with four replications in a completely randomized design. Glufosinate was applied at the V4 stage (4 nodes on the main stem with fully developed leaves beginning with the unifoliolate nodes) of soybean, and the variables analyzed were: soybean injury, chlorophyll index, plant height, dry and fresh matter of shoots and roots, wet nodules, dry nodules, and total number of nodules. It was found that for both cultivars, the maximum recommended glufosinate rate of 700 g of active ingredient (ai) ha-1 was safe, rates above 1,250 g ai ha-1 may interfere with development, especially biomass accumulation, and, in general, cultivar LL0291 exhibited more injuries than cultivar LL0767. The glufosinate-tolerant soybean (with pat gene) is a great option for farmers, but care should be taken with respect to rates above the maximum recommended in the package insert, so that there is no damage to soybean. © 2021 Friends Science Publishers","PeriodicalId":13769,"journal":{"name":"International Journal of Agriculture and Biology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41452498","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Impact of Seed Treatments with Fungal Biocontrol Agents on Enzymatic Activities and Phenolic Content of Soybean under Greenhouse and Field Conditions 温室和大田条件下真菌生物防治剂对大豆酶活性和酚类含量的影响
International Journal of Agriculture and Biology Pub Date : 2021-08-01 DOI: 10.17957/ijab/15.1837
A. Intisar
{"title":"Impact of Seed Treatments with Fungal Biocontrol Agents on Enzymatic Activities and Phenolic Content of Soybean under Greenhouse and Field Conditions","authors":"A. Intisar","doi":"10.17957/ijab/15.1837","DOIUrl":"https://doi.org/10.17957/ijab/15.1837","url":null,"abstract":"Fungi in the genus Trichoderma are widely used as biological control agents because they can suppress plant pathogens and activate plant defense systems. In the present study, efficacy of microbial antagonists viz., T. harzianum and T. viride or their combination was evaluated against the pathogenic fungus Macrophomina phaseolina and their effect on enzymatic activities and phenol content of soybean [Glycine max (L.) Merr.] plants. Soybean seeds were inoculated with T. harzianum and T. viride separately or in combination, and sown in pots under green house and under field conditions. Host enzymatic activities and phenol levels were measured at 14, 28 and 42 days after sowing (DAS) in both field and greenhouse experiments. Seed treatments with T. harzianum, T. viride or their combination increased peroxidase, polyphenol oxidase and β-1, 3-glucanase activities, and also the total phenol content in soybean leaves as compared to a non-treated control treatment. Concentration of peroxidase and β-1, 3-glucanasepeaked at 14 DAS and decreased thereafter in all the treatments under greenhouse and field conditions. All the treatments showed the highest levels of total phenols and polyphenol-oxidase at 28 DAS under both greenhouse and field conditions. At 14 DAS in both trials, the combination of T. viride + T. harzianum resulted in the highest level of peroxidase and β-1, 3-glucanase activities. This combination also resulted in the highest levels of total phenols and polyphenol oxidase content at 28 DAS. Our findings demonstrated that application of Trichoderma species as seed treatment has potential to trigger key mechanisms of systemically acquired resistance in soybean, and thereby enhanced efficacy of disease management tactics. © 2021 Friends Science Publishers","PeriodicalId":13769,"journal":{"name":"International Journal of Agriculture and Biology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48466704","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Seed Invigoration with Paclobutrazol Improves Seedling Growth, Physiological, Biochemical Attributes and Fruit Yield in Okra 多效唑补种改善秋葵幼苗生长、生理生化特性和果实产量
International Journal of Agriculture and Biology Pub Date : 2021-08-01 DOI: 10.17957/ijab/15.1835
R. Bashir
{"title":"Seed Invigoration with Paclobutrazol Improves Seedling Growth, Physiological, Biochemical Attributes and Fruit Yield in Okra","authors":"R. Bashir","doi":"10.17957/ijab/15.1835","DOIUrl":"https://doi.org/10.17957/ijab/15.1835","url":null,"abstract":"The production of okra is affected by improper seedling growth and abiotic factors like temperature and soil. This study was aimed to examine the effect of paclobutrazol (PBZ) seed priming on the growth, seedling vigor and yield of okra. A pot experiment was conducted in completely randomized design under natural conditions and seeds of two okra varieties were soaked in 0, 4, 8, 10 and 20 mg L-1 of PBZ solution. Results showed that PBZ seed soaking affected the germination rate and maximum reduction in germination rate was observed at 20 mg L-1 of PBZ. A gradual decrease in plant height was recorded in PBZ treated plants as compared to control. A decrease in plant height of PBZ treated plants was accompanied with an increase in plant biomass and number of branches. PBZ seed soaking resulted in darker green leaves with higher chlorophyll, protein and total free amino acid contents than control plants. The activity of antioxidant enzymes, peroxidase and superoxide dismutase was also higher in PBZ treated plants. The improvement in all vegetative and physiological parameters ultimately had a positive effect on yield. PBZ treated plants showed an increase in the number of pods and pod length while seed weight remained unaffected. Thus, seed priming with optimum doses of PBZ could improve the seedlings and yield of okra. © 2021 Friends Science Publishers","PeriodicalId":13769,"journal":{"name":"International Journal of Agriculture and Biology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43274755","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Assessment of Sugarcane Genotypes for Red Rot Resistance and Antifungal Activity of Rhizosphere Microbiota against Colletotricum falcatum 甘蔗抗红腐病基因型及根际微生物群抗镰状炭疽菌活性评价
International Journal of Agriculture and Biology Pub Date : 2021-08-01 DOI: 10.17957/ijab/15.1830
S. Aslam
{"title":"Assessment of Sugarcane Genotypes for Red Rot Resistance and Antifungal Activity of Rhizosphere Microbiota against Colletotricum falcatum","authors":"S. Aslam","doi":"10.17957/ijab/15.1830","DOIUrl":"https://doi.org/10.17957/ijab/15.1830","url":null,"abstract":"The aim of this study was to screen the potential of locally grown sugarcane genotypes for red rot resistance and activity of native microbial strains against the pathogen. Field trials were conducted with 70 genotypes for consecutive years and results showed that only two genotypes viz., SSRI-1 and CO-0238 showed resistant behavior towards red rot of sugarcane. The red rot pathogen Colletotrichum falcatum Went was characterized and pathogenicity tests on two susceptible genotypes (NSG-59 and CPSG-2923) showed high virulence of SUCF04 isolate to develop severe disease lesions. The native rhizospheric microbiota was screened for microbial consortia exhibiting fine antifungal activity against the highly virulent pathogenic strain. The antagonism assay exposed that 10 bacterial isolates out of 46 showed great potential for antifungal activity. The selected bacterial isolates revealed 68–99% pathogen inhibition during the assay. The fungal strains with biological control potential inhibited the pathogen growth by 20–80% and a group of three strains with more than 50% antifungal activity were characterized. The molecular characterization of these microbes revealed that the isolates were belonging to Bacillus subtilus, Pseudomonas putida, Pseudomonas fluorescence, Trichoderma harzianum and several other important taxa. This study revealed that only two sugarcane genotypes were found as resistant against red rot pathogen, while most of the genotypes showed susceptible to moderately susceptible response. Moreover, the native residential microbiota associated with sugarcane exhibited great antifungal potential and can be utilized for disease protection and improved crop productivity. © 2021 Friends Science Publishers","PeriodicalId":13769,"journal":{"name":"International Journal of Agriculture and Biology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47318737","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Antidiabetic, Antibacterial and Antioxidant Potential of Bioactive Peptides Extracted from the Germinated Seeds of Poncirus trifoliata 三叶Poncirus trifoliata发芽种子生物活性肽的抗糖尿病、抗菌和抗氧化潜力
International Journal of Agriculture and Biology Pub Date : 2021-08-01 DOI: 10.17957/ijab/15.1838
T. Mehmood
{"title":"Antidiabetic, Antibacterial and Antioxidant Potential of Bioactive Peptides Extracted from the Germinated Seeds of Poncirus trifoliata","authors":"T. Mehmood","doi":"10.17957/ijab/15.1838","DOIUrl":"https://doi.org/10.17957/ijab/15.1838","url":null,"abstract":"The seeds proteins are converted into the peptides during the germination through peptidases. These bioactive peptides are fractionated through the enzymes. The bioactive peptides are reported with the number of therapeutic effects like antioxidant, antibacterial, antidiabetic, anti-cancerous and antihypertensive etc. Synthetic drugs have number of adverse effects along with the therapeutic effects, but natural sources are safer and free from this problem. In the present research, Poncirus trifoliata seedling peptides extracts were used to determine antibacterial, antioxidant and anti-diabetic activities. The peptides extract showed substantial growth of inhibition against pathogenic strains including Escherichia coli, Staphylococcus aureus and Bacillus subtilis having the superior effect as compared to amoxicillin antibiotic as a standard drug. The IC50 value of P. trifoliata seeds peptides extract was 32.91 μg mL-1 as compared with standard (IC50) value 25.52 μg mL-1 of ascorbic acid showed good potential as antioxidant activity. The extracted germinated seeds peptides showed considerable antidiabetic activity in controlling the blood glucose level of alloxan induced diabetic rabbits to compare with standard drugs like glibenclamide. Thus, this study confirmed the antibacterial, antioxidant and antidiabetic effects of P. trifoliata seeds peptides is significantly higher as compared to pure drugs. Therefore, further complete separation and detailed investigation study is needed to isolate target peptides and revealed mechanism of action of bioactive peptides and formulation as a drug to replace the synthetics drugs with safe one. © 2021 Friends Science Publishers","PeriodicalId":13769,"journal":{"name":"International Journal of Agriculture and Biology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48735799","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Evaluation of Antagonistic Bacillus and Biochar against Soybean Root Rot Caused by Fusarium oxysporum 拮抗芽孢杆菌和生物炭对大豆尖孢镰刀菌根腐病的防治效果评价
International Journal of Agriculture and Biology Pub Date : 2021-08-01 DOI: 10.17957/ijab/15.1836
Fan Yang
{"title":"Evaluation of Antagonistic Bacillus and Biochar against Soybean Root Rot Caused by Fusarium oxysporum","authors":"Fan Yang","doi":"10.17957/ijab/15.1836","DOIUrl":"https://doi.org/10.17957/ijab/15.1836","url":null,"abstract":"Soybean root rot is a worldwide soil-borne fungal disease threatening soybean production, causing huge losses in yield and quality of soybean. Fusarium species are well recognized as the important causal agent of Fusarium root rot. To screen the beneficial Bacillus strains with capability of suppressing soybean root rot and evaluate the impact of Bacillus combined with biochar against soybean root rot, a pot experiment was conducted with different treatments. In this study, as potential biological control measures, antagonistic Bacillus isolates and different types of biochar were added to soil separately and excellent antagonistic strains mixed with bamboo biochar were applied to the soil. The results showed that seven Bacillus strains promoted the growth of soybean seedlings and reduced root rot severity by 33 to 61%. Bacillus amylolique faciens NH2 was associated with the lowest incidence of soybean root rot, indicating its bio-control potential. The value of plant height, root length and plant dry weight of soybean in the sterilized soil mixed with biochar separately treatment were superior to those of soybean in the inoculated with pathogen treatment, especially the bamboo biochar treatment reduced soybean root rot caused by Fusarium significantly and which bio-control efficacy was 77.41%. The soybean plants shoot and root dry weights in the biochar mixed B. amylolique faciens NH2 or B. subtilis DBK treatments were increased by17.1, 10.7% and 19.51, 19.64%, respectively, which were significantly higher than those of the inoculated pathogen treatment. Compared to antagonistic strain or biochar individual treatments, the disease control efficiency on soybean root rot was up to 64.86% in NH2 strain mixed with bamboo biochar treatment, which reduced root rot severity significantly and showed a synergistic effect. These results suggest that antagonistic Bacillus strains mixed with biochar can be used as an effective alternative in managing soybean root rot. © 2021 Friends Science Publishers","PeriodicalId":13769,"journal":{"name":"International Journal of Agriculture and Biology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41632334","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Antifungal Effects against Phaeoisariopsis personata under Greenhouse Conditions and Phytochemical Analysis of Jatropha curcas Leaf Extracts 温室条件下对风油草的抗菌作用及麻疯树叶提取物的植物化学分析
International Journal of Agriculture and Biology Pub Date : 2021-08-01 DOI: 10.17957/ijab/15.1829
Magreth Francis
{"title":"Antifungal Effects against Phaeoisariopsis personata under Greenhouse Conditions and Phytochemical Analysis of Jatropha curcas Leaf Extracts","authors":"Magreth Francis","doi":"10.17957/ijab/15.1829","DOIUrl":"https://doi.org/10.17957/ijab/15.1829","url":null,"abstract":"The study was conducted to test the antifungal efficacy of J. curcas leaf extracts against Phaeoisariopsis personata (causal pathogen for groundnut late leaf spot disease) under in vivo conditions, and to identify important phytochemical constituents exhibiting antifungal properties. The results showed that the greatest reduction of late leaf spot disease incidence was achieved by all the Jatropha curcas leaf extracts at the highest concentration (0.5 mg mL-1) as 36.89, 36.59 and 24.67% for chloroform, ethyl acetate and methanolic extracts, respectively. Subsequently, J. curcas leaf extracts treatments enhanced the growth and yield of groundnut compared with the control (untreated). The antifungal effects of J. curcas were supported by the presence of phytochemical constituents identified by GC-MS. Hexadecane; n-hexadecanoic acid; phenol, 2, 4 bis (-dimethylethyl); phytol and hexadecanoic methyl ester were detected as major phytocompounds in J. curcas leaf extracts that were possibly responsible for the antifungal activity. © 2021 Friends Science Publishers","PeriodicalId":13769,"journal":{"name":"International Journal of Agriculture and Biology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45805851","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Screening of Bacteria for Biosurfactants, Exopolysaccharides and Biofilms and their Impact on Growth Stimulation of Zea mays Grown under Petrol Stress 石油胁迫下玉米表面活性剂、胞外多糖和生物膜细菌的筛选及其对玉米生长的影响
International Journal of Agriculture and Biology Pub Date : 2021-08-01 DOI: 10.17957/ijab/15.1839
S. Habib
{"title":"Screening of Bacteria for Biosurfactants, Exopolysaccharides and Biofilms and their Impact on Growth Stimulation of Zea mays Grown under Petrol Stress","authors":"S. Habib","doi":"10.17957/ijab/15.1839","DOIUrl":"https://doi.org/10.17957/ijab/15.1839","url":null,"abstract":"Environmental stress imposed by petroleum hydrocarbons can compensate by use of auxin-producing bacteria having potential for biosurfactants production, to assist improved plants’ growth in petrol contaminated areas. In the present work, four auxin-producing bacteria were screened for biosurfactants, exopolysaccharides (EPS) and biofilms production capability. We hypothesized that Enterobacter sp. (A5C) was the most efficient strain with respect to biosurfactant production and can accumulate EPS as well as biofilms. This strain was attributed to exhibit emulsification index, percentage of hydrophobicity and percentage of hydrocarbon degradation more than 50%. Also, it produced 9.27 mg of EPS per 100 mL of culture while Fourier transform infrared spectroscopy (FTIR) confirmed the presence of alcoholic and carboxylic groups, ketone and sugars in it. Results of in vitro plant microbe interaction assay revealed its potential to stimulate the growth of Zea mays L. plants under 1 and 2% of petrol stress by improving physio-chemical attributes of treated plants, over control. Thus, it is concluded that the test organism i.e., Enterobacter sp. (A5C) might be involved in developing bacterial community (EPS and biofilms) that helped to colonize the bacteria to the plant roots and soil particles that ultimately encouraged the more access to nutrients and protection of plant roots from toxins in soil ecosystem. © 2021 Friends Science Publishers","PeriodicalId":13769,"journal":{"name":"International Journal of Agriculture and Biology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41677492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Molecular and Phenotypic Analysis of Bread Wheat Varieties in Relation to Durable Rust Resistance 面包小麦品种持久抗锈性的分子与表型分析
International Journal of Agriculture and Biology Pub Date : 2021-08-01 DOI: 10.17957/ijab/15.1841
Anisa Intikhab
{"title":"Molecular and Phenotypic Analysis of Bread Wheat Varieties in Relation to Durable Rust Resistance","authors":"Anisa Intikhab","doi":"10.17957/ijab/15.1841","DOIUrl":"https://doi.org/10.17957/ijab/15.1841","url":null,"abstract":"Global wheat production is constantly threatened by rust diseases. Identifying resistant genes is a useful tactic to control wheat rust pathogen. Twenty-six wheat varieties were screened with twelve Simple Sequence Repeats (SSR) markers to detect rust resistant genes and the efficacy of genes was validated through field testing. The alleles Lr32, Lr39, Lr50, SrCad and SrWeb were not amplified in the varieties included in this study. The SSR markers indicated that the varieties viz., Chakwal-97, Bakhar-2002 and Lasani-2008 had a combination of 02 slow rusting alleles (Lr46/Yr29 and Yr18/Lr34). The adult plant resistance (APR) allele Yr17 was less prevalent and found only in BWL-97. However, Noshera-96 had a slow rusting combination of Lr67/Yr46 and Lr46/Yr46 alleles. The Lr46/Yr29 identified in 50% of the varieties, Yr18/Lr34 in 19.23%, Lr32 in 11.54%, and multiple APR alleles in 19.32%. Their resistance was validated through a field trap nursery for 3 consecutive seasons. The slow rusting combination of Lr46/Yr29 and Yr18/Lr34 was comparatively more effective than Lr67/Yr46 and Lr46/Yr29 alleles under field conditions. The varieties Yecora-70, Lylpure-73 and Tandojam-83 showed highly susceptible phenotype. The varieties Chakwal-86, Pirsabak-2005, Fareed-2006, and Sehar-2006 showed resistant to moderately resistant phenotype at high-temperature adult-plant stage. The cluster diagram divided the varieties into two distinct clades. The clade II depicted the abundance of APR allele Lr46/Yr29. The varieties contain valuable sources of durable rust resistant alleles that can be exploited to deploy rust resistance in future wheat cultivars. It has been observed that the varieties approved for commercial cultivation after 1990s and onwards contain APR alleles. © 2021 Friends Science Publishers","PeriodicalId":13769,"journal":{"name":"International Journal of Agriculture and Biology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46756254","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
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