International Hepatology Communications最新文献_第6页

Circulating immune complexes that contain HCV but not GBV-C in co-infected hosts 在共感染宿主中含有丙型肝炎病毒但不含GBV-C的循环免疫复合物

International Hepatology Communications Pub Date : 1996-10-01 DOI: 10.1016/0928-4346(96)00316-7

Minako Hijikata, Shunji Mishiro

引用次数: 13

Genetic background of constitutional unconjugated hyperbilirubinemia 体质性非偶联高胆红素血症的遗传背景

International Hepatology Communications Pub Date : 1996-10-01 DOI: 10.1016/0928-4346(96)00313-1

Yukihiko Adachi , Toshinori Kamisako , Osamu Koiwai , Kazuo Yamamoto , Hiroshi Sato

{"title":"Genetic background of constitutional unconjugated hyperbilirubinemia","authors":"Yukihiko Adachi , Toshinori Kamisako , Osamu Koiwai , Kazuo Yamamoto , Hiroshi Sato","doi":"10.1016/0928-4346(96)00313-1","DOIUrl":"10.1016/0928-4346(96)00313-1","url":null,"abstract":"<div><p>Crigler-Najjar syndrome (types I and II) and Gilbert's syndrome are familial disorders associated with severe to mild unconjugated hyperbilirubinemia. In these conditions, the activity of bilirubin UDP-glucuronosyltransferase (UGT1<sup>∗</sup>1), which is located in the hepatocyte endoplasmic reticulum, is defective, and severely and moderately decreased, respectively. UGT1<sup>∗</sup>1 is derived from one of the UGT1 genes. It has a promoter containing a TATA box and consists of exon 1A (which is one of the individual first exons) and common exons 2–5. UGT1<sup>∗</sup>1 mRNA is formed by differential splicing of these exons. In recent years, gene analysis of these syndromes has been carried out, and genetic abnormalities have been clarified. Homozygous nonsense mutations, mis-sense mutations, and other relevant mutations of exons 1A-5 have been reported in almost all of the patients with Crigler-Najjar syndrome type I, while mainly homozygous mis-sense mutations of exons 1A, 2, and 5 have been reported in type II patients. Almost all patients with this syndrome (types I and II) show autosomal recessive inheritance. On the other hand, some patients with Gilbert's syndrome show heterozygous mis-sense mutations in exons 1A, 4, and 5, while others show homozygous 2-base pair-insertion mutation (TA) into the TATA box in the promoter region [A(TA)<sub>7</sub>TAA; normal: A(TA)<sub>6</sub>TAA]. The pattern of inheritance can be autosomal recessive or autosomal dominant. It has also been clarified that enzyme activity is lowered to about 30% (rather than 50%) by heterozygous mutations of the coding region, because of the occurrence of dominant negative mutation based on subunit-structure of the enzyme.</p></div>","PeriodicalId":13746,"journal":{"name":"International Hepatology Communications","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1996-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0928-4346(96)00313-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91155726","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 16

Changes of chronic hepatitis C virus anti-core and envelope antibodies by interferon therapy 干扰素治疗对慢性丙型肝炎病毒抗核心和包膜抗体的影响

International Hepatology Communications Pub Date : 1996-10-01 DOI: 10.1016/0928-4346(96)00312-X

Fumihiko Komine , Mituhiko Moriyama , Nakanobu Hayashi , Toshikazu Uchida , Toshio Shikata , Yasuyuki Arakawa

{"title":"Changes of chronic hepatitis C virus anti-core and envelope antibodies by interferon therapy","authors":"Fumihiko Komine , Mituhiko Moriyama , Nakanobu Hayashi , Toshikazu Uchida , Toshio Shikata , Yasuyuki Arakawa","doi":"10.1016/0928-4346(96)00312-X","DOIUrl":"10.1016/0928-4346(96)00312-X","url":null,"abstract":"<div><p>We measured the levels of three antibodies against core (JCC-2), E1 (E1–5), and <span><math><mtext>E2</mtext><mtext>NS1</mtext></math></span> (E2-1) hepatitis C virus (HCV) before and after interferon (IFN) therapy in 22 patients with chronic hepatitis C and assessed the relationship between the changes in these antibody titers and the response to IFN. The titers of serum JCC-2 and E2-1 antibodies before the IFN therapy did not show any significant relationship with IFN efficacy. In contrast, the titer of E1–5 antibody was significantly lower in the complete responder (CR) group than in the non-responder (NR) group. The JCC-2 antibody titer of the CR group to IFN therapy showed a significant decrease immediately, at 6 months and 1 year after the completion of IFN administration. However, that of the NR group either did not change or rose again 6 months or 1 year after therapy following an immediate short-term decrease. Thus the E1–5 antibody titer before IFN therapy and the JCC-2 antibody titer during IFN therapy seems to be a good indicator of IFN efficacy. In contrast, the E2-1 antibody did not correlate with IFN efficacy.</p></div>","PeriodicalId":13746,"journal":{"name":"International Hepatology Communications","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1996-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0928-4346(96)00312-X","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73956320","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of hepatic function using 99mTc-galactosyl human serum albumin (GSA) scintigraphy: comparison with antipyrine plasma clearance 用99mtc -半乳糖人血清白蛋白(GSA)显像评价肝功能:与安替比林血浆清除率的比较

International Hepatology Communications Pub Date : 1996-09-01 DOI: 10.1016/0928-4346(96)00302-7

Katsuyasu Kouda , Sang Kil Ha-Kawa , Yoshimasa Tanaka , Chizu Koreeda , Kyoichi Inoue

{"title":"Evaluation of hepatic function using 99mTc-galactosyl human serum albumin (GSA) scintigraphy: comparison with antipyrine plasma clearance","authors":"Katsuyasu Kouda , Sang Kil Ha-Kawa , Yoshimasa Tanaka , Chizu Koreeda , Kyoichi Inoue","doi":"10.1016/0928-4346(96)00302-7","DOIUrl":"10.1016/0928-4346(96)00302-7","url":null,"abstract":"<div><p><sup>99m</sup>Tc-galactosyl human serum albumin (GSA) is a new scintigraphy agent which binds specifically to asialoglycoprotein receptors on hepatocytes, and can be used to evaluate hepatic function. Indocyanine green (ICG) is hepatic blood flow-dependent, while antipyrine plasma clearance is independent of blood flow and thus antipyrine plasma clearance is an excellent parameter with which to assess liver function. In this study, we investigated the relationship between GSA examination and antipyrine plasma clearance to assess the usefulness of GSA in evaluating metabolic function of the liver. Studies were performed on 22 patients with liver diseases. Antipyrine plasma clearance measurement, ICG testing and biochemical analysis of blood were performed at the same time as GSA examination. GSA HH15 and antipyrine plasma clearance showed a good correlation (<em>r</em> = −0.702, <em>P</em> < 0.0005). On the other hand, ICGR15 showed a poor correlation with antipyrine plasma clearance (<em>r</em> = 0.449, <em>P</em> < 0.05). The results of this study suggest, that hepatic function determined using GSA is more closely related to the metabolic capacity of hepatocytes than to hepatic blood flow.</p></div>","PeriodicalId":13746,"journal":{"name":"International Hepatology Communications","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1996-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0928-4346(96)00302-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84170889","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Enhanced expression of type III γ-glutamyl transpeptidase messenger RNA in alcohol-fed rat liver 酒精喂养大鼠肝脏III型γ-谷氨酰转肽酶信使RNA的表达增强

International Hepatology Communications Pub Date : 1996-09-01 DOI: 10.1016/0928-4346(96)00306-4

Kazuhiko Sakamoto, Masayoshi Yamauchi, Yoshihiko Maezawa, Gotaro Toda

引用次数: 0

Predictive factors regarding the responsiveness to interferon therapy for patients with chronic hepatitis C: an assessment of the hypervariable region 1 quasispecies in genotype 1b of chronic hepatitis C 慢性丙型肝炎患者对干扰素治疗反应性的预测因素:慢性丙型肝炎1b基因型高变区1准种的评估

International Hepatology Communications Pub Date : 1996-09-01 DOI: 10.1016/0928-4346(96)00300-3

Kyoko Harada, Hiroshi Watanabe, Hiroshi Shijo, Makoto Okumura

{"title":"Predictive factors regarding the responsiveness to interferon therapy for patients with chronic hepatitis C: an assessment of the hypervariable region 1 quasispecies in genotype 1b of chronic hepatitis C","authors":"Kyoko Harada, Hiroshi Watanabe, Hiroshi Shijo, Makoto Okumura","doi":"10.1016/0928-4346(96)00300-3","DOIUrl":"10.1016/0928-4346(96)00300-3","url":null,"abstract":"<div><p>It is widely known that the hepatitis C virus (HCV) genotype 1b is generally resistant to interferon (IFN) therapy. Some reports have described various predictive factors regarding the responsiveness to IFN therapy. In this study, we evaluated predictive factors, the age of the patients, the level of HCV-RNA, the total dose of (IFN) and the level of serum alanine aminotransferase (s-ALT), potentially regarding the responsiveness to IFN therapy for patients with HCV genotype 1b. In addition, the hypervariable region 1 (HVR1) quasispecies in association with interferon inefficacy has been reported. We investigated the HVR1 using a polymerase chain reaction (PCR) — single strand conformation polymorphism (SSCP) analysis and thus attempted to determine whether or not an analysis of HVR1 quasispecies can possibly be used as a predictive factor for the responsiveness to IFN therapy. We studied 104 consecutive cases of chronic hepatitis C patients with HCV genotype 1b. Eighty-four patients from 104 could be assayed for the HVR1 quasispecies. Seventeen patients had complete responses (CR) to interferon therapy, 67 patients had non responses (NR). In addition, there was a significant difference between CR and NR groups regarding the level of HCV-RNA; in contrast, there was no differences for the age of the patients, the total dose of IFN and the HVR1 quasispecies. The above findings thus suggested that the level of HCV-RNA may be a predictive factor regarding the responsiveness to IFN therapy. However, the HVR1 quasispecies was not found to be a predictive factor.</p></div>","PeriodicalId":13746,"journal":{"name":"International Hepatology Communications","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1996-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0928-4346(96)00300-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75547721","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Immunohistochemical characterization of hepatic eosinophil cationic protein in primary biliary cirrhosis 原发性胆汁性肝硬化肝嗜酸性阳离子蛋白的免疫组化特征

International Hepatology Communications Pub Date : 1996-09-01 DOI: 10.1016/0928-4346(96)00298-8

Shingo Miyaguchi , Masaya Oda , Masaya Mori , Hiroyuki Imaeda , Yoshikazu Tsuzuki , Hidetsugu Saito , Kiyotaka Kamegay , Hiromasa Ishii

{"title":"Immunohistochemical characterization of hepatic eosinophil cationic protein in primary biliary cirrhosis","authors":"Shingo Miyaguchi , Masaya Oda , Masaya Mori , Hiroyuki Imaeda , Yoshikazu Tsuzuki , Hidetsugu Saito , Kiyotaka Kamegay , Hiromasa Ishii","doi":"10.1016/0928-4346(96)00298-8","DOIUrl":"10.1016/0928-4346(96)00298-8","url":null,"abstract":"<div><p>We have previously reported that serum eosinophil cationic protein (ECP_ levels are increased in primary biliary cirrhosis (PBC). However, little is known about the role of eosinophils in the pathogenesis of this disease. In this study, liver biopsy specimens obtained from 27 PBC patients (stage I: 11 cases; stage II: 9 cases; stage III: 5 cases; stage IV: 2 cases) were stained with a monoclonal antibody (EG2) produced against ECP in an attempt to elucidate whether EG2-positive eosinophils are involved in the destruction of bile duct in PBC. Needle liver biopsy specimens obtained from 24 patients with chronic hepatitis C (CHC) were used as controls. In PBC, more than 10 EG2-positive cells were noted per portal tract in 6 cases, less than 10 EG2-positive cells in 10 cases and none in 11 cases. In CHC, more than 10 EG2-positive cells were detected per portal tract in only one case, less than 10 EG2-positive cells in 3 cases and none in 20 cases. According to the semi-morphometric statistical analysis, the hepatic infiltration rate of EG2-positive activated eosinophils into the portal tract was significantly higher in PBC than in CHC. Based on the stages of PBC, the infiltration rate of EG2-positive cells was significantly higher in stages I and II than in stages III and IV. A significant correlation was found between EG2-positive cell infiltration and small round cell infiltration. These findings suggest that EG2-positive, activated eosinophils may be involved in the early stages of PBC when the inflammatory changes are localized in the portal tract. It is tentatively speculated that activated eosinophils may play a role possibly as effector cells in the immunopathogenesis of PBC.</p></div>","PeriodicalId":13746,"journal":{"name":"International Hepatology Communications","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1996-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0928-4346(96)00298-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77232010","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Hepatic apolipoprotein A-I, C-III and E mRNA expressions in human parenchymal liver diseases 肝载脂蛋白A-I、C-III和E mRNA在人实质性肝病中的表达

International Hepatology Communications Pub Date : 1996-09-01 DOI: 10.1016/0928-4346(96)00304-0

Noriko Fukushima, Kyosuke Yamamoto, Iwata Ozaki, Toshihiko Mizuta, Susumu Kajihara, Yoichi Setoguchi, Takahiro Sakai

引用次数: 0

Drug-induced hepatitis with severe cholestasis due to voglibose 药物性肝炎伴伏糖糖所致严重胆汁淤积

International Hepatology Communications Pub Date : 1996-09-01 DOI: 10.1016/0928-4346(96)00310-6

Toshikazu Masumoto, Maki Ishikawa, Yuusuke Yamauchi, Yoichi Hiasa, Kazuhiro Yamamoto, Hideto Iuchi, Keiji Ohkubo, S.M. Fazle Akbar, Kojiro Michitaka, Norio Horiike, Morikazu Onji

引用次数: 7

The effect of ethinyl-estradiol treatment on the lateral mobility of lipids and proteins in hepatocyte plasma membrane of male rats (FRAP studies on liver smears) 乙炔雌二醇对雄性大鼠肝细胞质膜脂质和蛋白质横向迁移的影响(肝涂片FRAP研究)

International Hepatology Communications Pub Date : 1996-09-01 DOI: 10.1016/0928-4346(96)00303-9

Kenichi Kitani, Imre Zs.-Nagy

引用次数: 7