International Hepatology Communications最新文献_第5页

Hepatitis C virus genotypes and co-infection with GB virus C in patients with anti-HCV-positive chronic liver disease in Jakarta, Indonesia 印度尼西亚雅加达抗hcv阳性慢性肝病患者的丙型肝炎病毒基因型和GB病毒C合并感染

International Hepatology Communications Pub Date : 1996-11-01 DOI: 10.1016/S0928-4346(96)00321-0

Laurentius A. Lesmana , H.Ali Sulaiman , H.M. Sjaifoellah Noer , Fumio Tsuda , Hiroaki Okamoto

引用次数: 3

Changes of depolymerized and polymerized tubulin proteins in the liver after taurocholic acid administration with or without colchicine pretreatment and cholestasis due to cytochalasin B and bile duct ligation in rats 牛牛胆酸加或不加秋水仙碱预处理、细胞松弛素B和胆管结肠炎后大鼠肝脏内解聚和聚合微管蛋白的变化

International Hepatology Communications Pub Date : 1996-11-01 DOI: 10.1016/S0928-4346(96)00329-5

Yohei Fukumoto , Keisuke Hino , Mitsuru Yasunaga , Kiwamu Okita

{"title":"Changes of depolymerized and polymerized tubulin proteins in the liver after taurocholic acid administration with or without colchicine pretreatment and cholestasis due to cytochalasin B and bile duct ligation in rats","authors":"Yohei Fukumoto , Keisuke Hino , Mitsuru Yasunaga , Kiwamu Okita","doi":"10.1016/S0928-4346(96)00329-5","DOIUrl":"10.1016/S0928-4346(96)00329-5","url":null,"abstract":"<div><p>Microtubules, which are components of the cytoskeleton, are the organelles involved in vesicular transport. In the hepatocyte, the microtubular network is presumed to be concerned with bile acid secretion, and there may also be some structural changes in cholestatic conditions. The polymerized tubulin levels were determined as microtubules in the liver by the radiochemical assay in rats after the administration of taurocholic acid, colchicine, taurocholic acid following the treatment of colchicine, cytochalasin B and common bile duct ligation. The polymerized tubulin levels were increased after the high dose administration of taurocholic acid, but were decreased after the treatment with colchicine. In cholestasis models induced by cytochalasin B and common bile duct ligation, the polymerized tubulin levels were markedly increased. These findings suggest the microtubule system plays an important role in the excretion of a large amount of bile acids into the bile, and that growth of the microtubular network occurs in cholestasis in order to promote the vesicular transport and to maintain the cell shape.</p></div>","PeriodicalId":13746,"journal":{"name":"International Hepatology Communications","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1996-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0928-4346(96)00329-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74617424","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Detection of telomerase activity in hepatocellular carcinoma by fluorescence-based TRAP method 基于荧光的TRAP法检测肝细胞癌端粒酶活性

International Hepatology Communications Pub Date : 1996-11-01 DOI: 10.1016/S0928-4346(96)00323-4

Hisashi Hisatomi, Yasuko Kawaoto, Yoshimi Ohmura, Miwako Kuwahara, Toshiyasu Yamauchi, Kazumasa Hikiji

{"title":"Detection of telomerase activity in hepatocellular carcinoma by fluorescence-based TRAP method","authors":"Hisashi Hisatomi, Yasuko Kawaoto, Yoshimi Ohmura, Miwako Kuwahara, Toshiyasu Yamauchi, Kazumasa Hikiji","doi":"10.1016/S0928-4346(96)00323-4","DOIUrl":"10.1016/S0928-4346(96)00323-4","url":null,"abstract":"<div><p>Telomeres are important for the function and stability of eukaryotic chromosomes. Telomeric DNA is shortened every time liver cells divide. Normal human liver cells have a limited proliferative capacity, but immortalized cells prevent the shortening of the end of chromosomes by the expression of telomerase, the enzyme that elongates telomeric DNA. Although many genetic alterations have been reported in hepatocellular carcinoma, whether all hepatocellular carcinoma are immortalized cells is not known. Therefore, the telomerase activity was analyzed in 38 frozen samples from human hepatocellular carcinoma by fluorescence-based TRAP Method. Telomerase activity was detected in 34 (89.47%) of 38 hepatocellular carcinoma tissues regardless of tumor size, but telomerase activity was detected in only 12 (31.58%) of 38 nontumor liver tissues from patients with hepatocellular carcinoma. Diagnosis of hepatocellular carcinoma is sometimes difficult when the hepatomas are small and are of the differentiated type. Our results showed that the expression of telomerase may be of great value in diagnosing hepatocellular carcinoma.</p></div>","PeriodicalId":13746,"journal":{"name":"International Hepatology Communications","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1996-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0928-4346(96)00323-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74531809","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 13

Detection of matrix metalloproteinase gene expression by reverse transcription-polymerase chain reaction in human hepatocellular carcinoma 逆转录-聚合酶链反应检测肝癌组织基质金属蛋白酶基因表达

International Hepatology Communications Pub Date : 1996-11-01 DOI: 10.1016/S0928-4346(96)00324-6

Iwata Ozaki, Kyosuke Yamamoto, Toshihiko Mizuta, Yoichi Setoguchi, Fumitaka Morito, Takahiro Sakai

引用次数: 7

GB virus C infection in fulminant and acute hepatitis GB病毒C型在暴发性和急性肝炎中的感染

International Hepatology Communications Pub Date : 1996-11-01 DOI: 10.1016/S0928-4346(96)00322-2

Kojiro Michitaka , Norio Horiike , Toshikazu Masumoto , Fazle S.M. Akbar , Naofumi Ohno , Morikazu Onji

{"title":"GB virus C infection in fulminant and acute hepatitis","authors":"Kojiro Michitaka , Norio Horiike , Toshikazu Masumoto , Fazle S.M. Akbar , Naofumi Ohno , Morikazu Onji","doi":"10.1016/S0928-4346(96)00322-2","DOIUrl":"10.1016/S0928-4346(96)00322-2","url":null,"abstract":"<div><p>To assess the presence of GB virus C (GBV-C) in fulminant and acute hepatitis, GBV-C-RNA was screened by reverse transcription-polymerase chain reaction (RT-PCR) in sera from 33 patients with acute and subacute liver diseases (fulminant hepatitis 14, late onset hepatic failure 2, acute hepatitis 17). GBV-C was detected in two of 14 patients with fulminant hepatitis, but not in any patient with acute hepatitis and late onset hepatic failure. Both GBV-C-positive patients were also infected with hepatitis B virus and both of them had a history of blood transfusion. None of the 15 patients with non-A, non-B, non-C (non-A-C) hepatitis were positive for GBV-C. From these data, it is suspected that infection of GBV-C is not very frequent in patients with non-A-C acute and fulminant hepatitis, and that blood transfusion is one of the important routes for GBV-C transfection, which correlates with the relatively high prevalence of patients co-infected with GBV-C and other blood-transmissible viruses such as hepatitis B and C virus.</p></div>","PeriodicalId":13746,"journal":{"name":"International Hepatology Communications","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1996-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0928-4346(96)00322-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88615307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

Fluorescence-labeled-hyaluronan loading test as an index of hepatic sinusoidal endothelial cell function in the rat 荧光标记透明质酸负荷试验作为大鼠肝窦内皮细胞功能的指标

International Hepatology Communications Pub Date : 1996-10-01 DOI: 10.1016/0928-4346(96)00318-0

Hitomi Nakabayashi, Hiroyuki Tsujii, Yasuyuki Okamoto, Hiroshi Nakano

{"title":"Fluorescence-labeled-hyaluronan loading test as an index of hepatic sinusoidal endothelial cell function in the rat","authors":"Hitomi Nakabayashi, Hiroyuki Tsujii, Yasuyuki Okamoto, Hiroshi Nakano","doi":"10.1016/0928-4346(96)00318-0","DOIUrl":"10.1016/0928-4346(96)00318-0","url":null,"abstract":"<div><p>Hyaluronan is an endogenous glycosaminoglycan whose clearance from plasma takes place mainly in the liver sinusoidal endothelial cells. Elevated levels of serum hyaluronan have been reported in various chronic liver diseases. This phenomenon may be caused by the impaired degradation of hyaluronan in damaged liver endothelial cells and also the enhanced production in proliferated connective tissue of the liver. The aim of this investigation was to evaluate the function of uptake of the exogenously administered hyaluronan from blood in the chronically injured liver of rats. We examined plasma clearance of fluorescein amine-labeled hyaluronan and its localization and accumulation in the liver. Plasma clearance of hyaluronan became slower with severity of chronic liver injury. Fluorescence localized in sinusoids was markedly reduced and the accumulation of fluorescence in the liver tissue was significantly decreased in the cirrhotic liver compared with the control liver. These results suggest that, in chronic liver injury, the impaired clearance of hyaluronan from blood is attributed to decreased hyaluronan uptake in the liver.</p></div>","PeriodicalId":13746,"journal":{"name":"International Hepatology Communications","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1996-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0928-4346(96)00318-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77767595","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Immunohistochemical study of expression of p-glycoprotein and mutant p53 protein in hepatocellular carcinoma from the viewpoint of modulation of transcriptional activity of MDR1 gene 从MDR1基因转录活性调控的角度研究肝细胞癌中p糖蛋白和突变型p53蛋白表达的免疫组化研究

International Hepatology Communications Pub Date : 1996-10-01 DOI: 10.1016/0928-4346(96)00314-3

Mariko Itsubo, Gotaro Toda

{"title":"Immunohistochemical study of expression of p-glycoprotein and mutant p53 protein in hepatocellular carcinoma from the viewpoint of modulation of transcriptional activity of MDR1 gene","authors":"Mariko Itsubo, Gotaro Toda","doi":"10.1016/0928-4346(96)00314-3","DOIUrl":"10.1016/0928-4346(96)00314-3","url":null,"abstract":"<div><p>Recently, it was reported that the promoter region of the human MDR1 gene was a target for the p53 tumor suppressor gene product; a mutant p53 specifically stimulated the MDR1 gene promoter. To elucidate how both proteins, <em>p</em>-glycoprotein and mutant p53 protein, correlate with each other in a human carcinoma cell of HCC in vivo, the expression of these proteins was investigated immunohistochemically. The results from 42 cases of HCC revealed that 28 cases (66.7%) had <em>p</em>-glycoprotein and 12 cases (28.6%) had mutant p53 protein. Regarding the positivity rate of each protein in each histologic differentiation, that of <em>p</em>-glycoprotein was higher in well or moderately differentiated grade than in poorly differentiated grade, whereas that of mutant p53 protein was lower in well or moderately differentiated grade. The distribution of each protein-positive cell was not always uniform through the tumor sections, and the locations of <em>p</em>-glycoprotein-positive carcinoma cells and mutant p53 protein-positive carcinoma cells were poorly coincident in the serial sections of the same case. It seems that p53 protein may not directly affect the expression of <em>p</em>-glycoprotein, therefore <em>p</em>-glycoprotein overexpression in HCC could not be explained only by the direct correlation between mutational inactivation of p53 and stimulation of transcriptional activity of MDR1 gene.</p></div>","PeriodicalId":13746,"journal":{"name":"International Hepatology Communications","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1996-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0928-4346(96)00314-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91082993","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Change in cerebral blood flow in an acute hepatitis C patient with depressive state while receiving interferon 急性丙型肝炎抑郁患者接受干扰素治疗时脑血流量的变化

International Hepatology Communications Pub Date : 1996-10-01 DOI: 10.1016/0928-4346(96)00311-8

M. Sasaki , M. Sata , H. Suzuki , Y. Uchimura , S. Murashima , K. Tanaka , N. Uchimura , J. Nakamura , M. Ishibashi , K. Tanikawa

引用次数: 3

Involvement of retinoblastoma protein in dibutyryl cyclic AMP-induced growth inhibition of the human hepatoma cells PLC/PRF/5 视网膜母细胞瘤蛋白参与二丁基环amp诱导的人肝癌细胞PLC/PRF/5的生长抑制

International Hepatology Communications Pub Date : 1996-10-01 DOI: 10.1016/0928-4346(96)00315-5

Yasuyuki Okamoto , Masaji Kikukawa , Hiroshi Nakano

引用次数: 0

Hepatocyte canalicular transport of organic anions in bile duct-ligated rats 胆管结扎大鼠肝细胞内有机阴离子的小管运输

International Hepatology Communications Pub Date : 1996-10-01 DOI: 10.1016/0928-4346(96)00317-9

Yukinhiko Adachi , Hirohisa Miya , Yoshifumi Okuyama , Toshinori Kamisako , Toshio Yamamoto

引用次数: 3