Fluorescence-labeled-hyaluronan loading test as an index of hepatic sinusoidal endothelial cell function in the rat

International Hepatology Communications Pub Date : 1996-10-01 DOI:10.1016/0928-4346(96)00318-0

Hitomi Nakabayashi, Hiroyuki Tsujii, Yasuyuki Okamoto, Hiroshi Nakano

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引用次数: 4

Abstract

Hyaluronan is an endogenous glycosaminoglycan whose clearance from plasma takes place mainly in the liver sinusoidal endothelial cells. Elevated levels of serum hyaluronan have been reported in various chronic liver diseases. This phenomenon may be caused by the impaired degradation of hyaluronan in damaged liver endothelial cells and also the enhanced production in proliferated connective tissue of the liver. The aim of this investigation was to evaluate the function of uptake of the exogenously administered hyaluronan from blood in the chronically injured liver of rats. We examined plasma clearance of fluorescein amine-labeled hyaluronan and its localization and accumulation in the liver. Plasma clearance of hyaluronan became slower with severity of chronic liver injury. Fluorescence localized in sinusoids was markedly reduced and the accumulation of fluorescence in the liver tissue was significantly decreased in the cirrhotic liver compared with the control liver. These results suggest that, in chronic liver injury, the impaired clearance of hyaluronan from blood is attributed to decreased hyaluronan uptake in the liver.

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荧光标记透明质酸负荷试验作为大鼠肝窦内皮细胞功能的指标

透明质酸是一种内源性糖胺聚糖，其清除主要发生在肝窦内皮细胞。血清透明质酸水平升高在各种慢性肝病中都有报道。这种现象可能是由于受损的肝内皮细胞对透明质酸的降解受损以及肝增殖结缔组织中透明质酸的产生增加所致。本研究的目的是评价外源性给药透明质酸在慢性损伤大鼠肝脏中的吸收功能。我们检测了荧光素胺标记透明质酸的血浆清除率及其在肝脏中的定位和积累。血浆透明质酸清除率随慢性肝损伤的严重程度而减慢。与对照肝相比，肝硬化肝中定位于窦状窦的荧光明显减少，荧光在肝组织中的积累也明显减少。这些结果表明，在慢性肝损伤中，血液中透明质酸清除受损归因于肝脏中透明质酸摄取减少。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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International Hepatology Communications

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