International Hepatology Communications最新文献_第7页

Changes of the serum level of IL-1 receptor antagonist (IL-1ra) in the patients with type C chronic hepatitis during interferon therapy 干扰素治疗期间丙型慢性肝炎患者血清IL-1受体拮抗剂(IL-1ra)水平的变化

International Hepatology Communications Pub Date : 1996-09-01 DOI: 10.1016/0928-4346(96)00308-8

Yasuhiko Ohkawa, Hiroki Takahashi, Mikio Zeniya, Yoshio Aizawa, Masami Sakaguchi, Gotaro Toda

{"title":"Changes of the serum level of IL-1 receptor antagonist (IL-1ra) in the patients with type C chronic hepatitis during interferon therapy","authors":"Yasuhiko Ohkawa, Hiroki Takahashi, Mikio Zeniya, Yoshio Aizawa, Masami Sakaguchi, Gotaro Toda","doi":"10.1016/0928-4346(96)00308-8","DOIUrl":"10.1016/0928-4346(96)00308-8","url":null,"abstract":"<div><p>Serum level of interleukin-1 receptor antagonist (IL-1ra) before and during interferon (IFN) therapy was measured by sandwich ELISA. Serum IL-1ra level was significantly increased in chronic hepatitis C (CH-C) patients as compared with healthy control subjects. There was no significant correlation between serum IL-1ra and ALT level. In CHC patients, IFN treatment elevated serum IL-1ra level significantly in 2 weeks after start of the treatment. The alteration of serum IL-1ra level during treatment of IFN was compared between complete responders (CR), in whom hepatitis C virus (HCV) was eradicated, transient responders (TR), whose ALT levels transiently decreased during the treatment, and relapsed and non-responders (NR), in whom the virus was not eradicated. In TR or NR, the level in 2 weeks after the start of treatment is significantly higher than that before the treatment and in 3 months after its start. In CR, however, this transient elevation of IL-1ra level was not observed. These changes of serum IL-1ra during IFN therapy might reflect the immunological or inflammatory changes of IFN-treated CHC patients and influence the efficacy of IFN therapy.</p></div>","PeriodicalId":13746,"journal":{"name":"International Hepatology Communications","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1996-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0928-4346(96)00308-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73045124","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Cellular distribution of glutathione S-transferase-P gene expression during rat hepatocarcinogenesis by diethylnitrosamine 二乙基亚硝胺致大鼠肝癌过程中谷胱甘肽s -转移酶- p基因表达的细胞分布

International Hepatology Communications Pub Date : 1996-09-01 DOI: 10.1016/0928-4346(96)00307-6

Tetsuo Kora , Motonobu Sugimoto , Kinji Ito

引用次数: 5

RP-8 and TRPM-2 gene expression is constitutive in rat liver and increased following galactosamine injury RP-8和TRPM-2基因的表达在半乳糖胺损伤后增加

International Hepatology Communications Pub Date : 1996-09-01 DOI: 10.1016/0928-4346(96)00301-5

Mark J. Czaja , Yang Xu , Teija Oinonen , Kai O. Lindros

{"title":"RP-8 and TRPM-2 gene expression is constitutive in rat liver and increased following galactosamine injury","authors":"Mark J. Czaja , Yang Xu , Teija Oinonen , Kai O. Lindros","doi":"10.1016/0928-4346(96)00301-5","DOIUrl":"10.1016/0928-4346(96)00301-5","url":null,"abstract":"<div><p>The contribution of apoptosis to hepatocyte cell death after rat liver injury from the hepatotoxin galactosamine (GalN) was examined. Histologic evidence of diffuse hepatocyte apoptosis existed after GalN administration. Since apoptosis is an active form of cell death usually requiring new gene expression, it was determined whether genes associated with apoptosis were expressed following GalN liver injury. RP-8 and TRPM-2, two genes associated with apoptosis in other organs, were expressed in normal liver, and their mRNA levels increased after GalN injury. These genes were expressed in hepatocytes without any intralobular zonal gradient in expression. In contrast to GalN liver injury, RP-8 and TRPM-2 levels were unchanged during the purely proliferative response following rat partial hepatectomy. These data suggest that hepatocytes constitutively express the genetic program for apoptosis including the RP-8 and TRPM-2 genes. The ability of toxic liver injury to further stimulate expression of these genes may lead to apoptotic hepatocyte cell death.</p></div>","PeriodicalId":13746,"journal":{"name":"International Hepatology Communications","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1996-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0928-4346(96)00301-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90961470","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lysine 190 is a covalent bilirubin-binding amino acid in human delta bilirubin 赖氨酸190是人三角胆红素中的一种共价胆红素结合氨基酸

International Hepatology Communications Pub Date : 1996-09-01 DOI: 10.1016/0928-4346(96)00309-X

Yukihiko Adachi , Naomi Murata , Susumu Tsunasawa , Toshinori Kamisako , Toshio Yamamoto

引用次数: 4

Biliary excretion of estrone metabolites in the rat 雌二醇代谢物在大鼠胆道的排泄

International Hepatology Communications Pub Date : 1996-09-01 DOI: 10.1016/0928-4346(96)00305-2

Hajime Takikawa, Naoyo Sano, Kazuko Tadokoro, Masami Yamanaka

{"title":"Biliary excretion of estrone metabolites in the rat","authors":"Hajime Takikawa, Naoyo Sano, Kazuko Tadokoro, Masami Yamanaka","doi":"10.1016/0928-4346(96)00305-2","DOIUrl":"10.1016/0928-4346(96)00305-2","url":null,"abstract":"<div><p>We previously reported that sulfobromophthalein infusion inhibited the biliary excretion of estradiol glucuronides, whereas dibromosulfophthalein had no effect. In the present study, we examined the biliary excretion of estrone metabolites in rats. Biliary excretion of a tracer dose of intravenously injected [<sup>3</sup>H]estrone to control rats or EHBR and effects of the infusion of sulfobromophthalein and dibromosulfophthalein were studied. Biliary excretion of estrone metabolites was delayed in EHBR. Analysis of the biliary estrone metabolites revealed a marked decrease of the glucuronides in EHBR. Sulfobromophthalein and dibromosulfophthalein infusion (0.2 μmol/min/100 g b.wt.) inhibited the biliary excretion of estrone metabolites. However, the decrease in biliary excretion of the glucuronides was observed only with sulfobromophthalein that is excreted mainly as the glutathione conjugate. These findings indicate that glucuronides of estrone and its metabolites are partly excreted into bile by a canalicular organic anion carrier for sulfobromophthalein-glutathione, but not for dibromosulfophthalein.</p></div>","PeriodicalId":13746,"journal":{"name":"International Hepatology Communications","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1996-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0928-4346(96)00305-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83739459","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Transforming growth factor-β (TGF-β)-receptor gene expression in cultured rat hepatocytes 转化生长因子β (TGF-β)受体基因在培养大鼠肝细胞中的表达

International Hepatology Communications Pub Date : 1996-07-01 DOI: 10.1016/0928-4346(96)00293-9

M. Zoremba, A. Gressner

引用次数: 1

Interferon-α plus indomethacin combined therapy in HBeAg positive chronic hepatitis B non-responder to a previous IFNα course : results of a pilot study 干扰素α +吲哚美辛联合治疗对先前IFNα疗程无反应的HBeAg阳性慢性乙型肝炎:一项初步研究的结果

International Hepatology Communications Pub Date : 1996-07-01 DOI: 10.1016/0928-4346(96)00286-1

P. Andreone, C. Cursaro, A. Gramenzi, R. Miniero, M. Bernardi, G. Gasbarrini

引用次数: 7

The grey edges of autoimmune hepatitis 自身免疫性肝炎的灰色边缘

International Hepatology Communications Pub Date : 1996-07-01 DOI: 10.1016/0928-4346(96)00297-6

I. Mackay

引用次数: 6

The grey edges of autoimmune hepatitis 自身免疫性肝炎的灰色边缘

International Hepatology Communications Pub Date : 1996-07-01 DOI: 10.1016/0928-4346(96)00297-6

Ian R. Mackay

{"title":"The grey edges of autoimmune hepatitis","authors":"Ian R. Mackay","doi":"10.1016/0928-4346(96)00297-6","DOIUrl":"https://doi.org/10.1016/0928-4346(96)00297-6","url":null,"abstract":"<div><p>Evolving knowledge on autoimmune hepatitis (AH) since the 1950s has highlighted its challenge to nosology. Descriptive diagnostic criteria have been provided recently from three sources: (i) the IASL revision of the 1976 Fogarty manual; (ii) the World Congress of Gastroenterology Terminology Working Party; (iii) the International Autoimmune Hepatitis Group (Brighton Report). Problems with definition of AH relate to (i) stage of disease, whether very early or very late, when markers are less evident; (ii) distinction between an ‘autoimmune state’ with minimal disease (‘chronic persistent hepatitis’) and progressive autoimmune hepatitis; (iii) overlaps and/or coexistences among autoimmune diseases affecting the liver or other tissues; (iv) possible subtypes, whether based on serological reactions or HLA <span><math><mtext>DR3</mtext><mtext>DR4</mtext></math></span> phenotypes. The particular problem presented by cases of HCV-associated chronic hepatitis with LKM autoantibodies is unresolved, but such cases are best placed in a virological category. There is a call for standardisation of testing and uniformity in expression of results for autoantibodies relevant to AH. The diagnostic utility of antibody to asialoglycoprotein receptor is promising despite difficulty in production, and insufficient sensitivity and specificity of current assays. Meanwhile the serological diagnosis of AH is necessarily based on carefully titrated reactivity by immunofluorescence for antibodies to nuclei or actin (Type 1), or liver-kidney microsomes (Type 2).</p></div>","PeriodicalId":13746,"journal":{"name":"International Hepatology Communications","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1996-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0928-4346(96)00297-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91611967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Transforming growth factor-β (TGF-β)-receptor gene expression in cultured rat hepatocytes 转化生长因子β (TGF-β)受体基因在培养大鼠肝细胞中的表达

International Hepatology Communications Pub Date : 1996-07-01 DOI: 10.1016/0928-4346(96)00293-9

Martin Zoremba , Axel M. Gressner

{"title":"Transforming growth factor-β (TGF-β)-receptor gene expression in cultured rat hepatocytes","authors":"Martin Zoremba , Axel M. Gressner","doi":"10.1016/0928-4346(96)00293-9","DOIUrl":"https://doi.org/10.1016/0928-4346(96)00293-9","url":null,"abstract":"<div><p>The expression of TGF-β receptor types I, II and III in freshly-isolated and monolayer cultured rat hepatocytes was studied at the RNA and protein level applying semiquantitative RT-PCR and immunocytochemistry (APAAP-, and fluorescence-staining, respectively). Transcripts of receptor types I, II and III were detected in cultured cells, the level of mRNA for the type I and type II receptors were present at each time point of culture and showed no change of expression during cultivation. The transcript of TGF-β type III receptor (betaglycan) was very sparse in freshly-isolated cells but showed a 5–6-fold increase up to 72 h of culture. Southern blotting and enzymatic cleavage of the PCR-amplificates proved the specificity of the PCR products. Immunochemical staining of cultured PC (48 h) with polyclonal anti-type I, -type II and -type III TGF-β receptor antibodies demonstrated the presence of the receptors with almost similar staining intensities. In freshly isolated PC staining for type III receptor was weakly. The results indicate a differential expression of TGF-β signaling receptors (I and II) and betaglycan co-receptor (III), respectively, in cultured PC.</p></div>","PeriodicalId":13746,"journal":{"name":"International Hepatology Communications","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1996-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0928-4346(96)00293-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91656424","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1