International Journal of Biochemistry最新文献_第7页

Dipeptidyl aminopeptidase activities of guinea-pig brain 豚鼠脑二肽氨基肽酶活性

International Journal of Biochemistry Pub Date : 1994-07-01 DOI: 10.1016/0020-711X(94)90085-X

Maria Smyth , Gerard O'Cuinn

引用次数: 17

Guidebook to the cytoskeletal and motor proteins 细胞骨架蛋白和运动蛋白指南

International Journal of Biochemistry Pub Date : 1994-06-01 DOI: 10.1016/0020-711X(94)90115-5

引用次数: 411

Human sciatic nerve phospholipid profiles from non-diabetes mellitus, non-insulin-dependent diabetes mellitus and insulin-dependent diabetes mellitus individuals. A 31P NMR spectroscopy study 非糖尿病、非胰岛素依赖型糖尿病和胰岛素依赖型糖尿病患者的坐骨神经磷脂谱。31P核磁共振波谱研究

International Journal of Biochemistry Pub Date : 1994-06-01 DOI: 10.1016/0020-711X(94)90105-8

David Driscoll , William Ennis , Patricio Meneses

{"title":"Human sciatic nerve phospholipid profiles from non-diabetes mellitus, non-insulin-dependent diabetes mellitus and insulin-dependent diabetes mellitus individuals. A 31P NMR spectroscopy study","authors":"David Driscoll , William Ennis , Patricio Meneses","doi":"10.1016/0020-711X(94)90105-8","DOIUrl":"10.1016/0020-711X(94)90105-8","url":null,"abstract":"<div><ul><li>1.1. Human sciatic nerve phospholipids obtained from non-diabetes mellitus (NDM), non-insulin-dependent diabetes mellitus (NIDDM), and insulin-dependent diabetes mellitus (IDDM) patients, after lower extremity amputation, were studied by 31P NMR spectrometry.</li><li>2.2. Nine phospholipids resonances in NDM and NIDDM groups were identified as followed: Ethanolamine plasmalogen (Epias, Chemical shift = 0.07δ); phosphatidylethanolamine (PE, 0.03δ); phosphatidylserine (PS, −0.05δ); sphingomyelin (SM, −0.09δ); lysophosphatidylcholine (LPC, −0.28δ); phosphatidylinositol (PI, −0.30δ); alkylacylphosphorylcholine (A1.PC, -0.78δ); phosphatidylcholine (PC −0.84δ), and an unknown resonance (U, 0.13δ).</li><li>3.3. In the IDDM group a resonance of lysophosphatidylinositol (LPI, 0.01δ) was detected in addition to the nine phospholipids listed above.</li><li>4.4. IDDM showed that PI and Al.PC were elevated and U was lower when compared with NDM; also, Eplas was lower when compared with NIDDM. PC was elevated and PS was lower when compared with both NDM and NIDDM.</li><li>5.5. Indices calculated from this data, showed that the choline ratio and choline/ ethanolamine ratio were elevated; while ethanolamine ratio, and myelin ratio were lower in IDDM group, when compared with both NDM and NIDDM groups.</li><li>6.6. Inactivation of the cholineacethyltransferase enzyme (ChAT) and enhancement of the phospholipidmethyltransferase enzyme (PLMT), secondary to an insulin deficiency, are proposed as an interpretation of these findings.</li></ul></div>","PeriodicalId":13733,"journal":{"name":"International Journal of Biochemistry","volume":"26 6","pages":"Pages 759-767"},"PeriodicalIF":0.0,"publicationDate":"1994-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0020-711X(94)90105-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19056806","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 12

Induction of heat shock protein and prevention of caerulein-induced pancreatitis by water-immersion stress in rats 水浸应激诱导大鼠热休克蛋白及预防小蛋白诱导的胰腺炎

International Journal of Biochemistry Pub Date : 1994-06-01 DOI: 10.1016/0020-711X(94)90110-4

Mlchiro Otaka , Hldeaki Itoh , Toshiyuki Kuwabara , Akira Zeniya , Shusei Fujimori , Setsuya Otani , Yohtalou Tashima , Osamu Masamune

引用次数: 55

Oligoclonal β-galactoside-binding immunoglobulins antigenically related to 14kda lectin in human serum and cerebrospinal fluid: Purification and characterization 人血清和脑脊液中与14kda凝集素抗原性相关的寡克隆β-半乳糖苷结合免疫球蛋白:纯化和表征

International Journal of Biochemistry Pub Date : 1994-06-01 DOI: 10.1016/0020-711X(94)90111-2

Raymonde Joubert-Caron , Michel Caron , Pascal Bochet , Ahmed Chadli , Pierre Delaporte , Edmond Schuller , Dominique Bladier

引用次数: 8

Ostrich α-amylase: Purification and characterization of the pancreatic isoenzymes 鸵鸟α-淀粉酶:胰腺同工酶的纯化与鉴定

International Journal of Biochemistry Pub Date : 1994-06-01 DOI: 10.1016/0020-711X(94)90113-9

Vaughan Oosthuizen, Ryno J. Naudé, Willem Oelofsen

引用次数: 3

Complement today 今天补

International Journal of Biochemistry Pub Date : 1994-06-01 DOI: 10.1016/0020-711X(94)90125-2

引用次数: 0

Cytochrome P450 细胞色素P450

International Journal of Biochemistry Pub Date : 1994-06-01 DOI: 10.1016/0020-711X(94)90117-1

引用次数: 0

Stearoyl-CoA desaturase activity in primary culture of chicken hepatocytes. influence of insulin, glucocorticoid, fatty acids and cordycepin 鸡肝细胞原代培养中硬脂酰辅酶a去饱和酶活性的研究。胰岛素、糖皮质激素、脂肪酸和虫草素的影响

International Journal of Biochemistry Pub Date : 1994-06-01 DOI: 10.1016/0020-711X(94)90107-4

Philippe Legrand, Daniel Catheline, Jean-Michel Hannetel, Philippe Lemarchal

{"title":"Stearoyl-CoA desaturase activity in primary culture of chicken hepatocytes. influence of insulin, glucocorticoid, fatty acids and cordycepin","authors":"Philippe Legrand, Daniel Catheline, Jean-Michel Hannetel, Philippe Lemarchal","doi":"10.1016/0020-711X(94)90107-4","DOIUrl":"10.1016/0020-711X(94)90107-4","url":null,"abstract":"<div><ul><li>1.1. Stearoyl-CoA desaturase (Δ9-desaturase) activity was measured in chicken primary hepatocytes, as a function of time in culture.</li><li>2.2. When using fasted donor animals, the desaturase activity was low at the beginning of culture and then increased steadily to a maximum value between 30 and 70 hr of culture. When hepatocyte cultures were prepared from fed animals, enzyme activity was high at the beginning of culture and maintained thereafter at similar values to those obtained in cultured hepatocytes from fasted animals after 30 hr of culture.</li><li>3.3. Insulin significantly enhanced enzyme activity when added to the culture medium at a 10−9M concentration, and a small stimulating effect was also observed with 10−6M dexamethasone.</li><li>4.4. Linoleic acid (0.5 mM) added to the culture medium as albuminic complex partly inhibited Δ9-desaturase activity.</li><li>5.5. Cordycepin (3' deoxyadenosine) decreased enzyme activity when present at a 3 μg/ml concentration in the culture medium.</li><li>6.6. Taken together, the induction of enzyme activity in culture, its impairment by cordycepin and response to insulin and linoleic acid strongly suggest that synthesis and translation of the Δ9-desaturase mRNA occur in chicken hepatocytes in primary culture, and that this cellular model may be a useful tool for further studies on Δ9-desaturase regulatory mechanisms.</li></ul></div>","PeriodicalId":13733,"journal":{"name":"International Journal of Biochemistry","volume":"26 6","pages":"Pages 777-785"},"PeriodicalIF":0.0,"publicationDate":"1994-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0020-711X(94)90107-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18911295","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 21

Initial characterization of human thymocyte sialidase activity: Evidence that this enzymatic system is not altered during the course of T-cell maturation 人胸腺细胞唾液酸酶活性的初步表征:该酶系统在t细胞成熟过程中没有改变的证据

International Journal of Biochemistry Pub Date : 1994-06-01 DOI: 10.1016/0020-711X(94)90106-6

A. Greffard, J.C. Pairon, H. Terzidis-Trabelsi, J.-M. Heslan, J. Bignon, C.R. Lambre, Y. Pilatte

{"title":"Initial characterization of human thymocyte sialidase activity: Evidence that this enzymatic system is not altered during the course of T-cell maturation","authors":"A. Greffard, J.C. Pairon, H. Terzidis-Trabelsi, J.-M. Heslan, J. Bignon, C.R. Lambre, Y. Pilatte","doi":"10.1016/0020-711X(94)90106-6","DOIUrl":"10.1016/0020-711X(94)90106-6","url":null,"abstract":"<div><ul><li>1.1. The sialidase activity of human thymocyte was examined by a fluorogenic assay.</li><li>2.2. These studies revealed that human thymocyte sialidase activity is essentially acid-active and membrane-bound since 59.6% and 33% of the total activity was recovered in the lysosome-enriched and microsomal fractions, respectively.</li><li>3.3. A weak activity was also detected in the cytosolic fraction.</li><li>4.4. However, the acidic optimum pH of this soluble sialidase was at variance with the general concept of mammalian soluble sialidases which are known to be optimally active at more neutral pH.</li><li>5.5. This acidic soluble sialidase seems to be a general characteristic of the human T-cell lineage since examination of mature circulating T-cells revealed that they contain a soluble sialidase activity similar to that observed in thymocytes.</li><li>6.6. Analysis of mature and immature thymocyte subpopulation obtained by differential PNA agglutination indicated that this enzymatic system was not altered during the course of thymic maturation.</li><li>7.7. These results suggest that unlike in T-cell activation where changes in the level of sialidase activity were shown to influence the extent of cell surface sialylation and thereby the cell physiology, this enzymatic system seems not to be involved in the fluctuation of cell surface sialic acid content observed during thymic maturation.</li></ul></div>","PeriodicalId":13733,"journal":{"name":"International Journal of Biochemistry","volume":"26 6","pages":"Pages 769-776"},"PeriodicalIF":0.0,"publicationDate":"1994-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0020-711X(94)90106-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19056807","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8