{"title":"豚鼠脑二肽氨基肽酶活性","authors":"Maria Smyth , Gerard O'Cuinn","doi":"10.1016/0020-711X(94)90085-X","DOIUrl":null,"url":null,"abstract":"<div><p></p><ul><li><span>1.</span><span><p>1. The subcellular distribution ofdipeptidyl aminopeptidase activities in guinea-pig brain was investigated. Our studies show that DAP I (Gly-Arg-NH-Mec hydrolase) type activity was found to have an acidic optimum and was associated with the nuclear pellet.</p></span></li><li><span>2.</span><span><p>2. No DAP II (Lys-Ala-NH-Mec hydrolase) type activity could be detected. Apparant hydrolysis was mainly due to aminopeptidase activity.</p></span></li><li><span>3.</span><span><p>3. DAP III (Arg-Arg-NH-Mec hydrolase) type activity is largely cytoplasmic, but there was evidence of a membrane form associated with the synaptosomes.</p></span></li><li><span>4.</span><span><p>4. DAP IV (Gly-Pro-NH-Mec hydrolase) type activity is present on the synaptosomal membrane, and also enriched in the microsomes. A soluble form of Gly-Pro-NH-Mec hydrolase activity is also present in the cytoplasm. Whether this activity is a DAP II or IV type activity is still yet to be determined.</p></span></li></ul></div>","PeriodicalId":13733,"journal":{"name":"International Journal of Biochemistry","volume":"26 7","pages":"Pages 913-921"},"PeriodicalIF":0.0000,"publicationDate":"1994-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0020-711X(94)90085-X","citationCount":"17","resultStr":"{\"title\":\"Dipeptidyl aminopeptidase activities of guinea-pig brain\",\"authors\":\"Maria Smyth , Gerard O'Cuinn\",\"doi\":\"10.1016/0020-711X(94)90085-X\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p></p><ul><li><span>1.</span><span><p>1. The subcellular distribution ofdipeptidyl aminopeptidase activities in guinea-pig brain was investigated. Our studies show that DAP I (Gly-Arg-NH-Mec hydrolase) type activity was found to have an acidic optimum and was associated with the nuclear pellet.</p></span></li><li><span>2.</span><span><p>2. No DAP II (Lys-Ala-NH-Mec hydrolase) type activity could be detected. Apparant hydrolysis was mainly due to aminopeptidase activity.</p></span></li><li><span>3.</span><span><p>3. DAP III (Arg-Arg-NH-Mec hydrolase) type activity is largely cytoplasmic, but there was evidence of a membrane form associated with the synaptosomes.</p></span></li><li><span>4.</span><span><p>4. DAP IV (Gly-Pro-NH-Mec hydrolase) type activity is present on the synaptosomal membrane, and also enriched in the microsomes. A soluble form of Gly-Pro-NH-Mec hydrolase activity is also present in the cytoplasm. Whether this activity is a DAP II or IV type activity is still yet to be determined.</p></span></li></ul></div>\",\"PeriodicalId\":13733,\"journal\":{\"name\":\"International Journal of Biochemistry\",\"volume\":\"26 7\",\"pages\":\"Pages 913-921\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1994-07-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/0020-711X(94)90085-X\",\"citationCount\":\"17\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"International Journal of Biochemistry\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/0020711X9490085X\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Journal of Biochemistry","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/0020711X9490085X","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 17
摘要
1.1. 研究了豚鼠脑内二肽基氨基肽酶活性的亚细胞分布。我们的研究表明，DAP I (Gly-Arg-NH-Mec水解酶)型活性具有酸性最佳，并且与核颗粒相关。未检测到DAP II (Lys-Ala-NH-Mec水解酶)型活性。表观水解主要是由于氨基肽酶的活性。DAP III (Arg-Arg-NH-Mec水解酶)型活性主要是细胞质，但有证据表明突触体相关的膜形式。DAP - IV (Gly-Pro-NH-Mec水解酶)型活性存在于突触体膜上，也在微粒体中富集。Gly-Pro-NH-Mec水解酶活性的可溶性形式也存在于细胞质中。该活性是DAP II型还是DAP IV型活性尚不确定。
Dipeptidyl aminopeptidase activities of guinea-pig brain
1.
1. The subcellular distribution ofdipeptidyl aminopeptidase activities in guinea-pig brain was investigated. Our studies show that DAP I (Gly-Arg-NH-Mec hydrolase) type activity was found to have an acidic optimum and was associated with the nuclear pellet.
2.
2. No DAP II (Lys-Ala-NH-Mec hydrolase) type activity could be detected. Apparant hydrolysis was mainly due to aminopeptidase activity.
3.
3. DAP III (Arg-Arg-NH-Mec hydrolase) type activity is largely cytoplasmic, but there was evidence of a membrane form associated with the synaptosomes.
4.
4. DAP IV (Gly-Pro-NH-Mec hydrolase) type activity is present on the synaptosomal membrane, and also enriched in the microsomes. A soluble form of Gly-Pro-NH-Mec hydrolase activity is also present in the cytoplasm. Whether this activity is a DAP II or IV type activity is still yet to be determined.
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